关键词: 地菍, 离体培养, 植株再生, 栽培, 观赏植物

Abstract: In vitro culture and plant regeneration of Melastoma dodecandrum by tissue culture using its
blade were studied. The results showed that the op timum medium for callus induction isMS supp lemented with
2, 4-D 1 mg·L ^-1 , 6-BA 0.2 mg·L^-1 , 5% sucrose and 0.6% agar , or MS with 2, 4-D 2 mg·L^-1 , 6-
BA 0.2 mg·L ^-1 , 5% sucrose and 0.6% agar. The optimum medium for the formation and growth of cluster
buds is MS with 6-BA 2 mg·L^-1 , NAA 0.3 mg·L^-1 , 5% sucrose and 0.6% agar. The optimum medium
for rooting is 1/2MS with NAA 1.5 mg·L^-1 , 2, 4-D 0.5 mg·L^-1 , 5% sucrose and 0.6% agar, or MS
with 6-BA 0.5 mg·L^-1 , NAA 2 mg·L^-1 , 5% sucrose and 0.6% agar. The cultivation for ornamental of
test-tube plantlets was also studied. The optimum temperature is 28 - 32℃. The optimum medium is 2/3 peat
soil and 1/3 vermiculite, or 2/3 humus and 1/3 vermiculite for pot cultivation. The best p roportion of N, P
and K is 114 ∶112 ∶1. The optimum soil is humus. The test2tube p lantlets can be cultivated as a pot flower
and ornamental lawn with high ornamental value.

Key words: Melastoma dodecandrum, In vitro culture, Regeneration, Cultivation, Ornamental