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园艺学报 ›› 2004, Vol. 31 ›› Issue (3): 378-380.

• 研究报告 • 上一篇    下一篇

不同镉水平对白菜生长及抗氧化酶活性的影响

孙光闻;朱祝军*;方学智   

  1. (浙江大学园艺系,杭州31002)
  • 收稿日期:2003-08-18 修回日期:2003-10-15 出版日期:2004-06-25 发布日期:2004-06-25
  • 通讯作者: 朱祝军

Efects of Diferent Cadmium Levels on the Growth and Antioxidant Enzymes in Brassica campestris L.ssp.chinensis (L.)Maidno

Sun Guangwen; Zhu Zhujun*; Fang Xuezhi   

  1. (Department of Horticulture,Zhejiang University,Hangzhou 310029,China)
  • Received:2003-08-18 Revised:2003-10-15 Online:2004-06-25 Published:2004-06-25

摘要: 不同镉(cd)水平对白菜[Brassica campestris L.ssp.chinensis (L.)Makino]两个品种生长及抗氧化酶活性研究结果表明:cd 1 mg·L-1 对两品种生长(除‘杭州油冬儿’地上部干质量)及叶绿素 含量无显著影响,cd 10 mg·L-1显著抑制植株生长(除‘沪青1号’根干质量),减少叶绿素含量。cd可诱导叶片中活性氧02-. 、H2O2的产生,引起膜脂过氧化产物丙二醛(MDA)的累积。Cd 1 mg’L 显著增加‘沪青1号’过氧化物酶(POD)、过氧化氢酶(CAT)及‘杭州油冬儿’抗坏血酸过氧化物酶(APx) 活性。cd 10 mg·L 显著降低两品种叶片超氧化物歧化酶( SOD ) 、CAT、APX及‘沪青1号’POD活性, 显著增加脱氢抗坏血酸还原酶(DR)和谷胱甘肽还原酶(GR)活性。

关键词: 白菜, 镉, 活性氧, 生长, 抗氧化酶

Abstract: A hydroponic experiment was carried out to study the effects of different Cd levels on the growth and the activities of antioxidant enzymes in two cultivars of Brassica campestris L.ssp.chinensis(L.) Makino.Cd 1 mg·L-1 didn’t significantly afect the growth and chlorophyll content of both cultivars (except the shoot dry mass of‘ 'Hangzhou Youdonger’),Cd 10 mg·L-1 significantly inhibited the growth and leaf chlorophyll content of both cuhivars(except the root dry mass of‘ 'Huqing 1’).,nle generation of 02-. and H2O2,content increased under Cd exposure,and mal0ndialdehyde(MDA)accumulated.The activities of catalase (CAT) and peroxidase (POD) of‘ Huqing 1’ and ascorbate peroxidase (APX) of ‘Hangzhou Youdonger’were significantly increased at Cd 1 mg·L-1.Cd 10 mg·L-1 significantly decreased the activities of superoxide dismutase (SOD),CAT and APX,and significantly enhanced t}Ie activities of dehydroascorbate reductase (DR) and glutathione reductase (GR) in both cuhivars.

Key words: Brassica campestris L. ssp.chinensis (L.) Makino, Cadmium, Active oxygen, Growth, Antioxidant enzyme

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