蝴蝶兰组培中pH和温度对外植体褐化的影响

园艺学报 ›› 2006, Vol. 33 ›› Issue (6): 1373-1376.

蝴蝶兰组培中pH和温度对外植体褐化的影响

赵伶俐^{1, 2};葛红¹;范崇辉²;印芳³;李秋香¹;周玉杰¹

(¹ 中国农业科学院蔬菜花卉研究所, 北京100081; ²西北农林科技大学园艺学院, 陕西杨凌712100; ³ 湖南农业大学植物激素重点实验室, 湖南长沙410128)

收稿日期:2006-02-17 修回日期:2006-04-22 出版日期:2006-12-25 发布日期:2006-12-25
通讯作者: 葛红

Effects of pH and Tempera ture on Brown ing of Pha laenopsis Explants Cultured in Vitro

Zhao Lingli^{1, 2};Ge Hong¹;Fan Chonghui2 , Yin Fang3 , Li Qiuxiang1 , and Zhou Yujie1

( ¹ Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081, China; 2College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China; 3 Key Laboratory of Phytohormones of Hunan Agricultural University, Changsha, Hunan 410128, China)

Received:2006-02-17 Revised:2006-04-22 Online:2006-12-25 Published:2006-12-25
Contact: Ge Hong

摘要/Abstract

摘要： 以蝴蝶兰R4品种叶片为外植体, 研究不同pH 培养基、培养温度对褐化率、多酚氧化酶( PPO) 活性和总酚含量的影响以及褐化率与PPO、总酚之间的相关性。结果表明: 培养基pH 6.5或培养温度20℃时外植体褐化率最低。在相同温度(25℃) 下, PPO活性、总酚含量高低并不与pH值大小成正比, 且褐化率与PPO无显著相关性, pH 5.0时, 褐化率与总酚含量显著相关; 在相同pH (6.0) 下, 温度越高, 褐化率越高, PPO活性越强, 总酚含量越高, 且20℃下, 褐化率与PPO、总酚含量分别达到极显著、显著相关, 30℃下, 褐化率与总酚含量显著相关。

关键词: 蝴蝶兰, 褐化, pH, 温度

Abstract: The variety‘R4’of Phalaenopsis was used for the study of pH and temperature effect on polyphenol oxidase ( PPO) activity, total phenolic content , browning rate of the exp lants, correlation of browning rate and PPO activity with total phenolic content. The browning rate was the lowest at pH6.5 or 20℃. Under the same temperature (25℃) , the PPO activity and total phenolic content didnpt have direct ratio with the bulk of pH, and there was no significant correlation between PPO activity and total phenolic content, but there was significant correlation between browning rate and total phenolic content at pH 5.0; Under the same pH (6.0) , PPO activity, total phenolic content and browning rate increased while the temperature rose, and there were the best significant correlation between browning rate and PPO activity at 20℃, significant correlation between browning rate and total phenolic content at 20℃ and 30℃.

Key words: Phalaenopsis, Browning, pH, Temperature

中图分类号:

S682.31

赵伶俐;;葛红;范崇辉;印芳;李秋香;周玉杰. 蝴蝶兰组培中pH和温度对外植体褐化的影响[J]. 园艺学报, 2006, 33(6): 1373-1376.

Zhao Lingli;;Ge Hong;Fan Chonghui;Yin Fang;Li Qiuxiang;and Zhou Yujie. Effects of pH and Tempera ture on Brown ing of Pha laenopsis Explants Cultured in Vitro[J]. ACTA HORTICULTURAE SINICA, 2006, 33(6): 1373-1376.

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