球根海棠离体培养的形态发生研究

园艺学报 ›› 2004, Vol. 31 ›› Issue (5): 694-696.

球根海棠离体培养的形态发生研究

刘福平;苏明华;章宁;林清洪

(福建省亚热带植物研究所, 厦门361006)

收稿日期:2003-12-17 修回日期:2004-07-23 出版日期:2004-10-25 发布日期:2004-10-25

In Vitro Morphogenesis of Begonia tuberhybrida

Liu Fuping;Su Minghua;Zhang Ning;Lin Qinghong

( Fujian Institute of Subtropical Botany , Xiamen 361006 , China)

Received:2003-12-17 Revised:2004-07-23 Online:2004-10-25 Published:2004-10-25

摘要/Abstract

摘要： 球根海棠外植体可在同一条件下同时诱导不定芽和胚状体, 经间接途径和直接途径的体细胞胚胎发生频率均很高, 体细胞胚为单细胞起源或多细胞起源。以再生嫩叶为继代中间体, 在较高温度(28 ℃) 下诱导, 可建立高效扩繁体系。纵切芽丛继代形成大量愈伤组织, 愈伤组织继代半年后几乎完全丧失分化能力, 发生激素驯化作用, 而且愈伤组织细胞染色体数目已发生广泛变异。

关键词: 球根海棠, 体细胞胚胎发生, 再生体系, 继代愈伤组织

Abstract: Organogenesis and somatic embryogenesis of Begonia tuberhybrida could carry out on the same culture conditions. Somatic embryos occurred from explant directly or indirectly and originated f rom single-cell or multi-cell. At higher induce temperature (28 ℃) , subculturing regenerated young leaf could establish high efficient regenerate system. Through subculturing cluster of buds , a large amount of callus formed , and the callus subcultured for half a year lost almostly differential ability. Observation showed callus was habituated by growth substance and there were wide-ranging variation in the number of chromosome in cells of the callus.

Key words: Begonia tuberhybrida, Somatic embryogenesis, Regenerate system, Subculture callus

中图分类号:

S 68

刘福平;苏明华;章宁;林清洪. 球根海棠离体培养的形态发生研究[J]. 园艺学报, 2004, 31(5): 694-696.

Liu Fuping;Su Minghua;Zhang Ning;Lin Qinghong. In Vitro Morphogenesis of Begonia tuberhybrida[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 694-696.

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