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园艺学报 ›› 2004, Vol. 31 ›› Issue (5): 673-675.

• 研究报告 • 上一篇    下一篇

大葱茎尖脱毒苗培养及增殖技术的研究

陈典;黄晓梅;姜玉东;梁艳   

  1. (东北农业大学园艺学院, 哈尔滨150030)
  • 收稿日期:2004-01-02 修回日期:2004-05-25 出版日期:2004-10-25 发布日期:2004-10-25

Studies on Culture and Propagation of Virus Elimination Seedings from WelshOnion Shoot-tip

Chen Dian;Huang Xiaomei;Jiang Yudong;Liang Yan   

  1. ( Department of Horticulture , Northeast Agriculture Universit , Harbin 150030 , China)
  • Received:2004-01-02 Revised:2004-05-25 Online:2004-10-25 Published:2004-10-25

摘要: 章丘大葱茎尖外植体启动培养基以MS + NAA 0.1 mg·L-1 + 2iP 0.4 mg·L-1最佳, 诱导率为100 % , 生根壮苗最佳培养基为1/ 2 MS + NAA 0.01 mg·L-1 + IBA 1.5 mg·L-1, 生根率100 %。驯化移栽成活率100 %。分化培养基以MS + NAA 0.1 mg·L-1 + 2iP 0.6 mg·L-1最佳, 丛生率100 % , 平均分化苗数6.8 。根尖染色体观察, 遗传表现稳定。

关键词: 大葱, 茎尖培养, 增殖, 驯化

Abstract: The results of virus elimination by shoot-tip culture of welsh onion commonly grown in Zhangqiu showed that the optimum medium for starting culture of shoot-tip was MS + NAA 0.1 mg·L-1 + 2iP 0.4 mg·L-1 , the rate of shoot induction was 100 %; the optimum medium for root induction was 1/ 2 MS + NAA 0.01 mg·L-1 + IBA 1.5 mg·L-1 , both rates of rooting were 100 % , and survival rate after habituation were 100 %。The optimum medium for shoot-tip to differentiate rank seedlings was MS + NAA 0.1 mg·L-1+ 2iP 0.6 mg·L-1 , and the average divided seedings number of each shoot-tip was 6.8 。The observation of cytology showed that this approach could maintain the stability of hereditary.

Key words: Welsh onion, Shoot-tip culture, Propagation, Habituation

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