降解沟叶结缕草枯草层的真菌菌株的初选

园艺学报 ›› 2004, Vol. 31 ›› Issue (5): 682-684.

降解沟叶结缕草枯草层的真菌菌株的初选

袁康培;徐礼根;冯明光

(浙江大学生命科学学院, 杭州310029)

收稿日期:2004-03-24 修回日期:2004-07-20 出版日期:2004-10-25 发布日期:2004-10-25

Prel iminary Screening of Fungal Strain Decomposing the Thatch of TurfgrassZoysia matrella

Yuan Kangpei;Xu Ligen ;Feng Mingguang

( College of Life Sciences , Zhejiang University , Hangzhou 310029 ,China)

Received:2004-03-24 Revised:2004-07-20 Online:2004-10-25 Published:2004-10-25

摘要/Abstract

摘要： 从沟叶结缕草枯草层、土壤和腐烂木头上共分离到45 株真菌菌株, 经刚果红透明圈法初筛和纤维素酶活力测定法复筛后共选出6 株可用于降解沟叶结缕草枯草层的菌株。它们对沟叶结缕草植株无致病作用, 其中HU43 ( Aspergillus awamori) 生长速度最快, 降解羧甲基纤维素钠的能力最强, 经3 d 固体发酵后纤维素酶活力达35.7 U·g^-1 , 经25 ℃恒温保湿7 d 后对沟叶结缕草枯草的降解率达24.34 %。

关键词: 沟叶结缕草, 真菌菌株, 筛选, 纤维素酶, 降解, 草坪, 枯草层

Abstract: Forty-five fungal strains were isolated from turf thatch of Zoysia matrella , soil and rotten wood , and six among them were selected out for decomposing the thatch of Zoysia matrella efficiently by preliminary screening with Congo-red-clear-zone method and rescreening with the assay of cellulase activities. Results showed that all of them had no pathogenicity to live turfgrass of Zoysia matrella , and the HU43 strain identified as Aspergillus awamori expressed the highest growth rate , as well as the greatest efficiency to decompose sodium carboxymethyl cellulose. The cellulase activity of HU43 reached 35.7 U·g^-1 after 3 days by solid state fermentation. After being incubated at 25 ℃under high moist condition for 7 days , 24.34 % of withered grass of Zoysia matrella had been decomposed by this strain.

Key words: Zoysia matrella, Fungal strain, Screening, Cellulase, Decompose, Turf thatch

中图分类号:

S 68

袁康培;徐礼根;冯明光. 降解沟叶结缕草枯草层的真菌菌株的初选[J]. 园艺学报, 2004, 31(5): 682-684.

Yuan Kangpei;Xu Ligen;Feng Mingguang. Prel iminary Screening of Fungal Strain Decomposing the Thatch of TurfgrassZoysia matrella[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 682-684.

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