小金海棠抗缺铁相关基因-Nramp基因片段的克隆

园艺学报 ›› 2004, Vol. 31 ›› Issue (3): 360-362.

小金海棠抗缺铁相关基因-Nramp基因片段的克隆

戚金亮^1,2;韩振海^1*;印莉萍^2*;许雪峰¹;张连忠³

(¹中国农业大学农学与生物技术学院果树分子生物学实验室，北京100094； ²首都师范大学生物系遗传与生物工程重点实验室，北京100037； ³山东农业大学园艺学院，泰安271018)

收稿日期:2004-09-23 修回日期:2004-02-23 出版日期:2004-06-25 发布日期:2004-06-25
通讯作者: 韩振海

Ooning of Nramp Gene Fragment Being Relating to Related to Resistance of Iron Deficiency Stress from Malus xiaojinensis

Qi Jinliang^1,2;Han Zhenhai^1*;Yin Liping^2* ;Xu Xuefeng¹;Zhang Lianzhong³

(¹ Molecular Biology Laboratory of Fruit Tree,College ofAgronomy and Biotechnology，China agric~ural University，Beijing 100094，China；²The Key Laboratory ofGenetics and Biotechnology，Biology Department ofCapital Normal University，Beijing 100037，China；³Department ofHorticulture，Shandong Agricultural University，Tai’an 271018，China)

Received:2004-09-23 Revised:2004-02-23 Online:2004-06-25 Published:2004-06-25

摘要/Abstract

摘要： 以高效抗缺铁苹果种小金海棠(Malus xiaojinensis Cheng et Jiang)为试材，以异源的小麦
Nramp基因为探针进行杂交分析。Southem杂交结果表明：小金海棠基因组中存在该家族基因。Northern杂交结果表明：在根和叶中，Nramp基因在正常供铁和缺铁胁迫下均能得以转录，且叶中的转录受缺铁胁迫的诱导而加强。通过PCR方法已从基因组DNA中扩增出了752 bp的特异性产物，该片段与水稻的OsNramp3具有83％的氨基酸序列同源性。

关键词: 小金海棠, 铁, Nramp, 杂交

Abstract: To initially study the role of Nramp gene in resisfng iron deficiency stress，we analyzed this gene in Malus xiaojinensis Cheng et Jiang by using blot hybridization technique with Nramp gene fragment of wheat as probe．Southern blot result suggested that there was Nramp gene in the genome of Malus xiaojinensis.Northern blot confirmed that transcription ofthe Nramp gene is induced in both roots and leaves and strengthened in leaves by iron stress.A 752 bp fragment of Nramp gene was obtained by PCR from the genomic DNA，
which has 83％ identities to OsNramp3 of rice in amino acid leve1．The results lead to the prediction that Nramp gene of Malus xiaojinensis may play a crucial role in resisting iron stress．

Key words: Malus xiaojinensis Cheng et Jiang, Iron, Nramp, Blot

中图分类号:

s661

戚金亮;韩振海;印莉萍;许雪峰;张连忠. 小金海棠抗缺铁相关基因-Nramp基因片段的克隆[J]. 园艺学报, 2004, 31(3): 360-362.

Qi Jinliang;Han Zhenhai;Yin Liping;Xu Xuefeng;Zhang Lianzhong. Ooning of Nramp Gene Fragment Being Relating to Related to Resistance of Iron Deficiency Stress from Malus xiaojinensis[J]. ACTA HORTICULTURAE SINICA, 2004, 31(3): 360-362.

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