反义ACO 基因导入获得瓶插寿命长的香石竹植株

园艺学报 ›› 2004, Vol. 31 ›› Issue (5): 633-636.

反义ACO 基因导入获得瓶插寿命长的香石竹植株

余义勋;包满珠^*

(华中农业大学园艺林学学院, 园艺植物生物学教育部重点实验室, 武汉430070)

收稿日期:2003-10-25 修回日期:2004-02-18 出版日期:2004-10-25 发布日期:2004-10-25
通讯作者: 包满珠

Carnation Flower Vase Life Prolonged by Transformation with Antisense ACCOxidase Gene

Yu Yixun;Bao Manzhu^*

( Key Laboratory of Horticultural Plant Biology , Ministry of Education , College of Horticulture and Forestry Sciences ,Huaz hong Agricultural University , Wuhan 430070 ,China)

Received:2003-10-25 Revised:2004-02-18 Online:2004-10-25 Published:2004-10-25
Contact: Bao Manzhu

摘要/Abstract

摘要： 通过农杆菌( Agrobacterium tumefaciens) 介导法, 利用香石竹叶片作外植体, 将反义香石竹ACC 氧化酶基因导入香石竹‘黄星’品种, 通过PCR 扩增、Southern blot 检测, 证明反义ACC 氧化酶基因已整合到香石竹基因组。研究发现, 叶龄、乙酰丁香酮等对转化率有着较大影响。通过叶片的两度选择分化, 大大减少了转化嵌合体比例。转基因植株在隔离条件下栽培, 开花正常, 切花在25 ℃条件下瓶插寿命较对照延长了4 d。

关键词: 香石竹, ACC 氧化酶(ACO) 基因, 遗传转化, 瓶插寿命

Abstract: Taking young leaf as explants , the carnation ( Dianthus caryophyllus L. ) cultivar‘Yellow Star’was transformed with antisense genomic DNA of ACC oxidase , the key enzyme in ethylene synthesis ,driven by the CaMV 35S promoter mediated by A grobacterium tumefacien. PCR analysis and Southern blotting detection showed that foreign gene was integrated into the carnation genome. It is demonst rated that both leaf age and the acetosyringone are effective factors that influences the differentiation fequency on selection medium. The chimera occurring rate was effectively decreased by two times regeneration of the leaf explants under selection. The transgenic plants were t ransplanted to soil and flowering normally in isolated greenhouse. The vase life of t ransgenic plant is 4 days longer than that of cont rol plants under 25 ℃.

Key words: Carnation, ACC oxidase (ACO) gene, Genetic transformation, Vase life

中图分类号:

S 68

余义勋;包满珠. 反义ACO 基因导入获得瓶插寿命长的香石竹植株[J]. 园艺学报, 2004, 31(5): 633-636.

Yu Yixun;Bao Manzhu. Carnation Flower Vase Life Prolonged by Transformation with Antisense ACCOxidase Gene[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 633-636.

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