关键词: 草莓, 果实, 衰老, 微粒体膜, Ca²⁺-ATPase, 膜脂过氧化

Abstract: Changes in activities of Ca²⁺-ATPase and SOD , O-·2 production rate and MDA content in microsomal membrane of strawberry fruit stored at normal temperature (25 ℃) and cold temperature (4 ℃) were investigated. The results showed that the activities of total microsomal membrane Ca²⁺-ATPase, plasmolemma Ca²⁺-ATPase , mitochondria-Ca²⁺-ATPase and tonoplast-Ca²⁺-ATPase , and of SOD , and O^-·₂ production rate all increased at first and then decreased , and MDA content changed in the contrary trend during senescence in fruit stored at 4 ℃. The changing trends in Ca²⁺-ATPase activities and O^-·₂ production rate of microsomal membrane in fruit stored at 25 ℃were consistent with the fruit stored at 4 ℃. However , a gradual decrease in SOD activity and increase in MDA content were found during room temperature storage. It is suggested that the fruit senescence was closely correlated with the damage of cytoplasmic Ca²⁺ homeostasis and the enhancement of membrane lipid
peroxidation in strawberry fruit

Key words: Strawberry, Fruit, Senescence, Microsomal membrane, Ca²⁺-ATPase, Lipid peroxidation