源于大蒜花苞的生长素对蒜薹伸长的促进作用

园艺学报 ›› 2006, Vol. 33 ›› Issue (6): 1241-1245.

源于大蒜花苞的生长素对蒜薹伸长的促进作用

叶梅荣^{1, 2};甘立军¹;夏凯¹

(¹ 南京农业大学生命科学学院, 江苏南京210095; ² 安徽科技学院生命科学学院, 安徽凤阳233100)

收稿日期:2005-12-05 修回日期:2006-04-11 出版日期:2006-12-25 发布日期:2006-12-25
通讯作者: 夏凯

Auxin Derived from Inflorescence Dominates Garlic Bolt Elongation in Allium sativum

YeMeirong^{1, 2};Gan Lijun¹;Xia Kai¹

(¹College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; ²College of Life Science, Anhui Science and Technology University, Fengyang, Anhui 233100, China )

Received:2005-12-05 Revised:2006-04-11 Online:2006-12-25 Published:2006-12-25
Contact: Xia Kai

摘要/Abstract

摘要： 研究源于大蒜花苞的生长素对蒜薹伸长生长的影响。结果表明: 切去大蒜花苞(去顶) 明显抑制蒜薹的伸长生长, 说明蒜薹的伸长受其上部的花苞控制。去顶后, 在切口位置分别施用吲哚乙酸( IAA)和赤霉素(GA₃ ) , 均能提高去顶蒜薹的伸长率; 外源IAA和GA₃ 也能促进离体蒜薹切段的伸长; 在上述过程中IAA的促进作用高于GA₃。在不去顶的花苞基部施用生长素极性运输抑制剂2, 3, 5 - 三碘苯甲酸(2,3, 5-triiodobenzoic acid, TIBA) , 降低了下部蒜薹的伸长率; TIBA也能抑制离体蒜薹切段的伸长。内源激素分析表明, 去顶后蒜薹中IAA和GA_{1 +3}含量急剧下降, 为正常留花苞蒜薹的14.00%和13.76%, 表明花苞是维持蒜薹中高水平的IAA和活性GA所必需的。外源IAA处理显著增加去顶蒜薹和离体蒜薹切段GA₁₊₃的含量。说明来自花苞的IAA能维持蒜薹中活性GA的水平, 由此共同发挥出调控蒜薹伸长的作用。

关键词: 大蒜, 吲哚乙酸, 赤霉素, 蒜薹

Abstract: We studied the effect of auxin derived from inflorescence on garlic bolt elongation in Allium sativum L. The decapitation (removing inflorescence) induced the decrease of garlic bolt elongation, indicating that garlic bolt elongation was controlled by the inflorescence. After decap itation, application of exogenous either IAA or GA₃ at the excised site increased garlic bolt elongation rate significantly. Again, both exogenous IAA and GA₃ promoted elongation of excised garlic bolt segments. IAA showed a stronger p romotion effect than GA₃ in these processes. Application of 2, 3, 5-triiodobenzoic acid ( TIBA) , a polar auxin transport inhibitor, at the base of inflorescence decreased garlic bolt elongation rate. TIBA also inhibited elongation of excised
garlic bolt segments. Assay of plant hormones revealed that decapitation reduced both endogenous IAA and GA_{1+ 3} content in garlic bolt to a level of 14.00% and 13.76% of the undecapitated control, respectively,which indicated that inflorescence should be important to maintain high level of IAA and active GA in garlic bolt. Applied IAA increased GA₁₊₃ content in both decapitated garlic bolt and excised garlic bolt segments dramatically. All together, these data suggest that IAA derived from inflorescence dominate garlic bolt elongation by maintaining content of active GA in garlic bolt.

Key words: Allium sativum L., Indole-3-acetic acid ( IAA), Gibberellin (GA₃), Garlic bolt

中图分类号:

S633.4

叶梅荣;;甘立军;夏凯. 源于大蒜花苞的生长素对蒜薹伸长的促进作用[J]. 园艺学报, 2006, 33(6): 1241-1245.

YeMeirong;;Gan Lijun;Xia Kai. Auxin Derived from Inflorescence Dominates Garlic Bolt Elongation in Allium sativum[J]. ACTA HORTICULTURAE SINICA, 2006, 33(6): 1241-1245.

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