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园艺学报 ›› 2004, Vol. 31 ›› Issue (5): 589-592.

• 研究论文 • 上一篇    下一篇

‘罗田甜柿’胚乳培养获得十二倍体再生植株

陈绪中;罗正荣*   

  1. (华中农业大学园艺林学学院, 武汉430070)
  • 收稿日期:2003-10-14 修回日期:2004-02-20 出版日期:2004-10-25 发布日期:2004-10-25

Dodecaploid Plantlets Regenerated from Endosperm Culture of ‘Luotiantianshi’Persimmon

Chen Xuzhong;Luo Zhengrong*   

  1. ( College of Horticulture and Forestry , Huazhong Agricultural University , Wuhan 430070 , China)
  • Received:2003-10-14 Revised:2004-02-20 Online:2004-10-25 Published:2004-10-25

摘要: 取‘罗田甜柿’自然授粉后约70 d 的胚乳, 采用不同细胞分裂素和生长素组合进行离体培
养。结果表明: 以1/ 2MS (1/ 2N ) + ZT (玉米素) 1.1 mg/ L + 2,4-D 1.0 mg/ L + CH (水解酪蛋白) 1000mg/ L 诱导产生的愈伤组织易获得再生植株; 形态学和细胞学检测确认再生植株中存在2n=12x=180 的个体。该十二倍体植株对中国原产甜柿的遗传改良可能具有潜在的应用价值。

关键词: 柿, 甜柿, 胚乳培养, 多倍体, 十二倍体, 再生植株

Abstract: Endosperms of‘Luotiantianshi’persimmon ( Diospyros kaki Thunb. ) were cultured with after different auxin and cytokinin concent ration combination open pollination 70 days. Result s showed that the 1/ 2MS (1/ 2N) medium contained 2,4-D 1.0 mg/ L + ZT 1.1 mg/ L + CH 1000 mg/ L was better for
inducing callus to from regenerated plantlets , Stomatal characteristics、flow cytometric analysis and chromosome counting proved dodecaploid (2n=12x= 180) presence in those regenerated plantlets. The regenerated dodecaploid plants could be used as parental material in breeding program.

Key words: Persimmon, Nonast ringent persimmon, Endosperm culture, Polyploid, Dodecaploid, Regenerated plantlet

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