秋水仙素离体诱导同源四倍体青花菜

园艺学报 ›› 2009, Vol. 36 ›› Issue (11): 1681-1684.

秋水仙素离体诱导同源四倍体青花菜

张蜀宁*; 张丽丽;唐君;孔艳娥;侯喜林

(南京农业大学园艺学院, 作物遗传与种质创新国家重点实验室, 南京210095)

收稿日期:2009-06-15 修回日期:2009-09-07 出版日期:2009-11-25 发布日期:2009-11-25
通讯作者: 张蜀宁

In Vitro Induction of Autotetraploid Broccoli with Colchicine

ZHANG Shu-ning*;ZHANG Li-li;TANG Jun;KONG Yan-e;HOU Xi-lin

(College Horticulture State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agriculture University, Nanjing 210095, China)

Received:2009-06-15 Revised:2009-09-07 Online:2009-11-25 Published:2009-11-25
Contact: ZHANG Shu-ning

摘要/Abstract

摘要： 以青花菜品种‘海兹’高频再生试管苗为外植体, 利用秋水仙素离体诱导四倍体。结果表明: 用含秋水仙素200 mg·L ^{- 1}的MS + 0.1 mg·L ^-1NAA + 6.0 mg·L ^{- 1} 6-BA液体培养基处理2 cm长的再生苗48 h, 每个外植体平均可产芽2.1个, 再生植株变异率为83.33% , 四倍体诱导率达79.17%。与二倍体相比, 四倍体植株叶片、花冠、气孔等均表现巨大性; 流式细胞仪倍性鉴定显示, 对照DNA相对含量为200, 四倍体再生植株为400。四倍体根尖染色体2n = 4x = 36。

关键词: 青花菜, 离体培养, 秋水仙素, 四倍体

Abstract: In this study, the tube plantlets of broccoliwere cultured on Murashige and Skoog fluid medium supplemented 6-BA and NAA in combinationswith different concentrations of colchicine and treatment time to induce tetraploid. The results showed that the treatmentwith 200 mg· L - 1 colchicines inMS + 011 mg·L ^{- 1} NAA + 6.0 mg·L ^{- 1} 6-BA for 48 hours yielded doubled chromosomes at highest rates, the number of shoots per exp lant was 2.1, the variation efficiency was 83.33% , and the variational rate of tetrap loid was 79.17 %. Compared with the dip loid, the tetrap loid plants possessed gigantism in leaves, floral, stomata of plantlets. The flow cytometry was used to identify the ploidy of induced plants. The result suggested that the relative DNA content of controlwas 200 and the number of chromosome was 2n = 2x = 18, and tetrap loid relative DNA contentwas 400 and the number of chromosome was 2n = 4x = 36. The identification of the chromosome analysis indicated the regeneration plantletswere tetraploid.

Key words: Brassica oleracea L. var. italica Planch., in vitro culture, colchicines, tetraploid

中图分类号:

S 635

张蜀宁;张丽丽;唐君;孔艳娥;侯喜林. 秋水仙素离体诱导同源四倍体青花菜[J]. 园艺学报, 2009, 36(11): 1681-1684.

ZHANG Shu-ning;ZHANG Li-li;TANG Jun;KONG Yan-e;HOU Xi-lin. In Vitro Induction of Autotetraploid Broccoli with Colchicine[J]. ACTA HORTICULTURAE SINICA, 2009, 36(11): 1681-1684.

[1]	张爱玲, 涂红艳, 肖望, 钟晓晴, 陆秋婵, 成丽萍, 林晓萍, 麦钰玲. 白姜花二倍体与四倍体切花形态与显微结构变化观察[J]. 园艺学报, 2023, 50(2): 345-358.
[2]	黄静静, 刘伟, 刘玉梅, 韩风庆, 方智远, 杨丽梅, 庄木, 张扬勇, 吕红豪, 王勇, 季家磊, 李占省. Ogura CMS青花菜育性恢复材料创制及其遗传背景研究[J]. 园艺学报, 2022, 49(3): 533-547.
[3]	唐征, 徐谦, 张小玲, 刘庆, 朱世杨, 钟伟杰. 青花菜新品种‘瓯绿75’[J]. 园艺学报, 2021, 48(S2): 2835-2836.
[4]	党江波, 党江波, 韩国辉, 李彩, 向素琼, 郭启高, 梁国鲁. 柑橘倍性操作技术与育种现状、难点及展望[J]. 园艺学报, 2021, 48(4): 791-810.
[5]	童俊, 叶要妹, 陈法志, 周媛, 许林, 毛静, 董艳芳, 徐冬云. 四倍体紫薇新品种‘紫馨’和‘银蝶’[J]. 园艺学报, 2020, 47(S2): 3111-3112.
[6]	吕学思，张栋，闵芮涵，李世琦，李铮，任梓铭，夏宜平. 外源蔗糖对离体条件下换锦花小鳞茎发生的影响[J]. 园艺学报, 2020, 47(8): 1475-1489.
[7]	王春夏，张梦迪，王锦霞，王志平，孙红梅. 朱顶红无菌苗叶片高效再生体系[J]. 园艺学报, 2020, 47(2): 301-309.
[8]	王晓庆1，骆军1,*，施春晖1，张学英1，董建忠2. 四倍体梨新品种‘众翠’[J]. 园艺学报, 2019, 46(S2): 2735-2736.
[9]	宋鑫，谭丰全，张苗，蔡元康，郭大勇，解凯东，伍小萌，郭文武*. ‘纽荷尔’脐橙与‘尤力克’柠檬种间体细胞杂种的代谢特征分析[J]. 园艺学报, 2019, 46(1): 37-46.
[10]	刘莉莉1，江汉民1，单晓政1，姚星伟1，文正华1，牛国保1，张小丽1，马云生2，孙德岭1,*. 青花菜新品种‘领秀3号’[J]. 园艺学报, 2018, 45(S2): 2747-2748.
[11]	刘平1,2，王玖瑞2,3，刘志国1,2，王利虎1,2，刘嘉彬4，徐娟2，石庆华1，刘孟军1,2,*. 四倍体早熟鲜食枣新品种‘虹光’[J]. 园艺学报, 2018, 45(7): 1423-1424.
[12]	王宇，蒋炜，闫凯，周雯文，王志敏，魏大勇，汤青林. 青花菜AGL18及HDA9与开花信号整合子互作研究[J]. 园艺学报, 2018, 45(12): 2383-2394.
[13]	梁森林，党江波，梁国鲁，郭启高*. 天然四倍体枇杷‘B431’减数分裂观察及育性分析[J]. 园艺学报, 2018, 45(10): 1895-1904.
[14]	李占省，张黎黎，张小丽，舒金帅，苏彦宾，孙继峰，刘玉梅*，方智远，杨丽梅，庄木，张扬勇，吕红豪. 转Cry1Ac青花菜回交后代鉴定方法研究[J]. 园艺学报, 2018, 45(1): 97-108.
[15]	张勇，马建祥，李好，魏春华，杨建强，张显*. 甜瓜四倍体新品种‘多甜1号’[J]. 园艺学报, 2017, 44(2): 403-404.