车轮梅茎段高效再生体系的建立

园艺学报 ›› 2008, Vol. 35 ›› Issue (6): 895-898.

车轮梅茎段高效再生体系的建立

刘娟旭^1,2;刘玲¹;余义勋^1*;王静¹;黄现宝¹

（¹华南农业大学园艺学院，广州，510642； ²University of Florida，Institute of Food and Agricultural Sciences， Apopka, FL 32703-8504，U.S.A.）

收稿日期:2007-12-17 修回日期:2008-02-27 出版日期:2008-06-25 发布日期:2008-06-25
通讯作者: 余义勋

Establishment of A Highly Efficient System of Shoot Regeneration from Stem Segment Explants of Rhaphiolepis indica L.

LIU Juan-Xu^1,2, LIU Ling¹, YU Yi-Xun^1*, WANG Jing¹ ,and HUANG Xian-Bao¹

（¹College of Horticulture, South China Agricultural University, Guangzhou, 510642； ²University of Florida, Institute of Food and Agricultural Sciences, Apopka, FL 32703-8504，America.）

Received:2007-12-17 Revised:2008-02-27 Online:2008-06-25 Published:2008-06-25
Contact: YU Yi-Xun

摘要/Abstract

摘要： 以车轮梅 (Rhaphiolepis indica L.)茎段为外植体。探讨基本培养基种类、植物生长调节剂组合、蔗糖浓度和AgNO3等因素对茎段器官发生和植株再生的影响。结果表明：MS+6-BA 2.0 mg·L^-1 +NAA 0.5 mg·L^-1 + AgNO3 1.0 mg·L^-1+蔗糖40 g·L^-1培养基最适合不定芽的分化和增殖，不定芽分化率达90%以上，平均每外植体分化不定芽数达5.38个。不定芽可在1/2MS培养基中有效伸长，适宜生根培养基为1/2MS+NAA 1.0 mg·L^-1，生根率达到100%。

关键词: 车轮梅, 茎段, 植物生长调节剂, 不定芽再生

Abstract: An efficient plant regeneration protocol for Rhaphiolepis indica L. was achieved via organogenesis direct formation on stem segment explants in vitro. Several factors affecting in vitro regeneration of R. indica, such as basic medium, different hormones combination, concentrations of sucrose and AgNO3, were examined. The optimum medium for shoot regeneration was MS medium containing 2.0 mg·L^-1 6-BA, 0.5 mg·L^-1 NAA, 1.0 mg·L^-1 AgNO₃and 40 g·L^-1 sucrose. 90% of adventitious shoot regeneration frequency with 5.38 shoots per explants was obtained. Excised shoots were effectively elongated in 1/2 MS medium. Elongated shoots of 3.0 cm rooted when they were transferred to 1/2MS medium containing1.0 mg·L^-1 NAA after 40 days and developed into healthy plantlets, which resulted in a rooting rate of 100%.

Key words: Rhaphiolepis indica L., stem segment, plant growth regulator, shoot regeneration

中图分类号:

S 685.12

刘娟旭;刘玲;余义勋;王静;黄现宝. 车轮梅茎段高效再生体系的建立[J]. 园艺学报, 2008, 35(6): 895-898.

LIU Juan-Xu;LIU Ling;YU Yi-Xun;WANG Jing;and HUANG Xian-Bao. Establishment of A Highly Efficient System of Shoot Regeneration from Stem Segment Explants of Rhaphiolepis indica L.[J]. ACTA HORTICULTURAE SINICA, 2008, 35(6): 895-898.

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