黄瓜抗炭疽病相关基因AFLP标记的SCAR转换

园艺学报 ›› 2008, Vol. 35 ›› Issue (1): 123-126.

黄瓜抗炭疽病相关基因AFLP标记的SCAR转换

李淑菊;王惠哲^*;霍振荣;管炜

(天津科润黄瓜研究所，天津 300192)

收稿日期:2007-09-07 修回日期:2007-11-20 出版日期:2008-01-25 发布日期:2008-01-25
通讯作者: 王惠哲

Development of SCAR Marker Linked to Cucumber Anthracnose Resistance-related Gene from an AFLP Marker

LI Shu-ju, WANG Hui-zhe^*, HUO Zhen-rong, and GUAN Wei

(Tianjin Kernel Cucumber Research Institute, Tianjin 300192,China)

Received:2007-09-07 Revised:2007-11-20 Online:2008-01-25 Published:2008-01-25

摘要/Abstract

摘要： 将黄瓜抗炭疽病相关基因的一个共显性AFLP标记成功地转换成了简单实用的SCAR标记。对AFLP标记片段进行序列测定，根据序列特点设计了特异的SCAR引物，引物长18～21 bp。PCR结果表明，引物对（1）可以扩增出131 bp和125 bp两条带，引物对（2）可以扩增出178 bp和172 bp两条带，分别为抗炭疽病的特征带和感炭疽病的特征带。将该两个标记命名为SCEM131/125和SCEM178/172，两对引物在F2单株和抗感病材料验证中符合率高达97.27%，可以用于黄瓜炭疽病的分子鉴定。

关键词: 黄瓜, 炭疽病抗性相关基因, AFLP标记, SCAR标记

Abstract: The co-dominant AFLP marker of Anthracnose Resistance-related Gene of Cucumber was converted into co-dominant SCAR marker successfully. After sequencing of the AFLP marker fragment, two pairs of primers were designed according to the nucleotide sequence. PCR results from cucumber genomic DNA showed the two primer pairs, (1) and (2), could amplify two band types with the size of 131bp/125 bp and178bp /172 bp respectively. Both 131 bp band and 178 bp band were the character band of anthracnose resistance phenotype. These SCAR markers were designated as SCEM131/125、SCEM178/172。Anthracnose resistance of F2 and some known cucumber lines were tested by SCAR markers, and the results indicated that the resistance tested was consisitent with their field performance by 97.27%, which further confirmed the feasibility of the SCAR markers in MAS for cucumber anthracnose.

Key words: cucumber, anthracnose resistance-related gene, AFLP marker, SCAR marker

中图分类号:

S 642.2

李淑菊;王惠哲;霍振荣;管炜. 黄瓜抗炭疽病相关基因AFLP标记的SCAR转换[J]. 园艺学报, 2008, 35(1): 123-126.

LI Shu-ju;WANG Hui-zhe;HUO Zhen-rong;and GUAN Wei . Development of SCAR Marker Linked to Cucumber Anthracnose Resistance-related Gene from an AFLP Marker[J]. ACTA HORTICULTURAE SINICA, 2008, 35(1): 123-126.

[1]	罗天宽, 吴海涛, 张圣美, 黄宗安, 孙继, 水德聚, 陈先知. 黄瓜新品种‘瓯翠1号’[J]. 园艺学报, 2022, 49(S2): 125-126.
[2]	王鹤冰, 向华丰, 陈新中, 张生, 张洪成. 华南型黄瓜新品种‘新燕095’[J]. 园艺学报, 2022, 49(S1): 79-80.
[3]	许春梅, 张作标, 柳景兰, 王昕, 杨龙, 赵丹, 刘思宇, 贾云鹤, 孟雪娇, 崔嵩岑. 黄瓜新品种‘绿春2号’[J]. 园艺学报, 2022, 49(S1): 81-82.
[4]	张利东, 黄洪宇, 孔维良, 李加旺, 李愚鹤, . 华北型黄瓜新品种‘津优355’[J]. 园艺学报, 2022, 49(S1): 83-84.
[5]	王惠哲, 杨瑞环, 邓强, 曹明明, 李淑菊, . 抗黑星病黄瓜新品种‘津冬369’[J]. 园艺学报, 2022, 49(S1): 85-86.
[6]	聂鑫淼, 栾恒, 冯改利, 王超, 李岩, 魏珉. 硅营养和嫁接砧木对黄瓜幼苗耐冷性的影响[J]. 园艺学报, 2022, 49(8): 1795-1804.
[7]	韩鲁杰, 冯一清, 杨秀华, 张宁, 毕焕改, 艾希珍. 有机肥化肥配施对大棚黄瓜根区土壤与根系特征的影响[J]. 园艺学报, 2022, 49(5): 1047-1059.
[8]	权建华, 段誉, 罗天, 袁强, 齐鑫, 王勤礼. 黄瓜新品种‘裕研9号’[J]. 园艺学报, 2022, 49(3): 703-704.
[9]	宋蒙飞, 查高辉, 陈劲枫, 娄群峰. 黄瓜株型性状分子基础研究进展[J]. 园艺学报, 2022, 49(12): 2683-2702.
[10]	王团团, 缑晨星, 夏磊, 朱拼玉, 李季, 陈劲枫. 黄瓜10个基因型材料外植体内源激素水平及比例对其离体再生的影响[J]. 园艺学报, 2021, 48(9): 1731-1742.
[11]	赵昌博, 郑世伟, 边婷, 王爽, 张小兰, 富宏丹, 孙周平, 李天来. 日光温室黄瓜不同连作茬次土壤的中量与微量元素含量变化[J]. 园艺学报, 2021, 48(9): 1805-1814.
[12]	胡伟, 刘昱希, 赵勤政, 陈劲枫, 娄群峰. 黄瓜—酸黄瓜异附加系的创制与鉴定[J]. 园艺学报, 2021, 48(7): 1349-1358.
[13]	程凤, 宋蒙飞, 曹蕾, 张孟茹, 杨志歌, 陈劲枫, 娄群峰. 黄瓜的一个中短果突变体基因的初步定位[J]. 园艺学报, 2021, 48(7): 1359-1370.
[14]	贾会霞, 李锡香, 宋江萍, 林毓娥, 张晓辉, 邱杨, 阳文龙, 娄群峰, 王海平. 黄瓜核心种质白粉病抗性的全基因组关联分析[J]. 园艺学报, 2021, 48(7): 1371-1385.
[15]	林毓娥, 梁肇均, 何晓明, 王瑞, 刘文睿, 吴廷全, 彭庆务, 江彪, 王敏, 谢大森. 华南型黄瓜新品种‘力丰2号’[J]. 园艺学报, 2021, 48(7): 1431-1432.