醉蝶花离体培养植株再生研究

园艺学报 ›› 2004, Vol. 31 ›› Issue (1): 115-116.

醉蝶花离体培养植株再生研究

王健美;高辉;刘志斌;吴俊;李旭锋^*

(四川大学生命科学学院, 成都610064)

收稿日期:2003-04-14 修回日期:2003-07-01 出版日期:2004-02-25 发布日期:2004-02-25
通讯作者: 李旭锋

Studies on Plant Regeneration of Cleome spinosa Jacq. in Vitro

Wang Jianmei ;Gao Hui;Liu Zhibin;Wu Jun;Li Xufeng^*

( College of life science , Sichuan University , Chengdu 610064 , China)

Received:2003-04-14 Revised:2003-07-01 Online:2004-02-25 Published:2004-02-25
Contact: Li Xufeng

摘要/Abstract

摘要： 诱导醉蝶花下胚轴和子叶形成愈伤组织, 以MS + 6-BA 0.5 mg/L + NAA 0.5 mg/L + KT 0.5 mg/L培养基中子叶的出愈率最高, 达到100 % , 下胚轴出愈率最高只有93 %。MS + 6-BA 2 mg/L + NAA 0.05 mg/L最适合于芽的诱导, 诱导率达到100 %。再生苗在MS + NAA 0.1 mg/L 培养基中生根完成植株再生。带单个侧芽的茎作为外植体接种在MS + 6-BA 1 mg/L + NAA 0.05 mg/L 培养基中, 一个月以后形成无数丛生芽。

关键词: 醉蝶花, 离体培养, 植株再生

Abstract: Taking MS as basic media , adopting 6-BA , NAA , KT of different concentration compoundings to induce callus from cotyledons and hypocotyls of Cleome spinosa Jacq. , the result showed that the mediumof MS +6-BA 0.5 mg/ L + NAA 0.5 mg/ L + KT 0.5 mg/ L was the best to induce callus from cotyledons with a rate of 100 %frequency , and the highest rate of hypocotyl callus inducement was 93 %. And the differentiation medium MS + 6-BA 2 mg/ L + NAA 0.05 mg/ L was the best to induce buds and had a 100 % plant regeneration. And it was for rooting media , MS + NAA 0.1 mg/ L ,to produce roots from seedings. Taking the stems with single bud as explants on MS + 6-BA 1 mg/ L + NAA 0.05 mg/ L , it was found that there are lots of buds after a month.

Key words: Cleome spinosa Jacq., Culture in vitro, Plant regeneration

中图分类号:

S 68

王健美;高辉;刘志斌;吴俊;李旭锋. 醉蝶花离体培养植株再生研究[J]. 园艺学报, 2004, 31(1): 115-116.

Wang Jianmei;Gao Hui;Liu Zhibin;Wu Jun;Li Xufeng. Studies on Plant Regeneration of Cleome spinosa Jacq. in Vitro[J]. ACTA HORTICULTURAE SINICA, 2004, 31(1): 115-116.

[1]	吕学思，张栋，闵芮涵，李世琦，李铮，任梓铭，夏宜平. 外源蔗糖对离体条件下换锦花小鳞茎发生的影响[J]. 园艺学报, 2020, 47(8): 1475-1489.
[2]	雅蓉1，徐伟荣2,3,4,5,*，张莹1，夏思琪2，张宁波2,3,5. 褪黑素对‘无核白’葡萄体细胞胚的诱导作用[J]. 园艺学报, 2020, 47(5): 953-962.
[3]	王春夏，张梦迪，王锦霞，王志平，孙红梅. 朱顶红无菌苗叶片高效再生体系[J]. 园艺学报, 2020, 47(2): 301-309.
[4]	刘星月, 朱强龙, 李慧英, 孙静宇, 李必聪, 张宏玉, 黄英金, 方加军, 周庆红, . 红芽芋脱毒试管芋诱导及植株再生[J]. 园艺学报, 2020, 47(12): 2427-2438.
[5]	肖望，涂红艳，张爱玲. 白姜花胚性愈伤组织的诱导与植株再生体系的建立[J]. 园艺学报, 2016, 43(8): 1605-1612.
[6]	雷颖, 任一杰, 沈晓燕. 松潘乌头嫩茎高效再生体系的建立[J]. 园艺学报, 2015, 42(7): 1393-1399.
[7]	涂红艳, 肖望, 邓崇会. 红姜花体细胞胚胎发生及植株再生的研究[J]. 园艺学报, 2014, 41(10): 2139-2146.
[8]	孙华彩, 赵兰勇, 张玲, 丁一鸣. 玫瑰杂交花柱半离体培养研究[J]. 园艺学报, 2012, 39(6): 1183-1190.
[9]	王文和;吴禄平;赵玉萍;王超. 草莓未受精子房离体雌核发育的研究[J]. 园艺学报, 2011, 38(8): 1455-1461.
[10]	张启香;胡恒康;;王正加;袁佳;万俊丽;黄坚钦;. 山核桃间接体细胞胚发生和植株再生 [J]. 园艺学报, 2011, 38(6): 1063-1070.
[11]	姜新超;刘春;明军;穆鼎;袁素霞. 岷江百合悬浮细胞系的建立及植株再生[J]. 园艺学报, 2011, 38(2): 327-327–334.
[12]	廉玉姬;林光哲;赵小梅. 大白菜、青花菜和叶用芥菜体细胞杂交种形态学与细胞学特性鉴定[J]. 园艺学报, 2011, 38(11): 2099-2110.
[13]	李艳娜;尉义明;胡桂兵;陈厚彬;徐春香. 香蕉胚性细胞悬浮系玻璃化法超低温保存及再生植株遗传稳定性分析[J]. 园艺学报, 2010, 37(6): 899-905.
[14]	周索;杜丽;褚学英;包满珠;. 翠菊‘花束绯红’叶片的植株再生体系建立[J]. 园艺学报, 2010, 37(10): 1667-1672.
[15]	宁国贵;吕海燕;张俊卫;包满珠. 梅花不同外植体离体培养及体细胞胚诱导植株再生[J]. 园艺学报, 2010, 37(1): 114-120.