银条茎尖培养快繁及离体根状茎的诱导

园艺学报 ›› 2004, Vol. 31 ›› Issue (1): 106-108.

银条茎尖培养快繁及离体根状茎的诱导

张国裕¹;程智慧^1*;李娟¹;周文安²;王新华³

(¹ 西北农林科技大学园艺学院, 杨凌712100 ; ² 陕西省武功烟叶复烤厂, 武功712200 ; ³ 西安大鹏生物科技股份有限公司, 西安710075)

收稿日期:2003-03-24 修回日期:2003-06-06 出版日期:2004-02-25 发布日期:2004-02-25
通讯作者: 程智慧

Studies on Tip Meristem Culture Rapid Propagation and Induction of in VitroStolon of Manyflower Betony ( Stachys floridana Schuttl . ex Benth. )

Zhang Guoyu¹;Cheng Zhihui¹;Li Juan¹;Zhou Wenpan²;Wang Xinhua³

(¹ College of Horticulture , North-West Sci-Tech University of Agricultural and Forestry , Yangling 712100 , China ; ² Wugong Tobacco Re-baking Factory , Wugong 712200 , China , ³ Xi-an Dapeng Biological Sci-Tech Stock Sharing Company , Ltd. Xipan , 710075 ,China)

Received:2003-03-24 Revised:2003-06-06 Online:2004-02-25 Published:2004-02-25
Contact: Cheng Zhihui

摘要/Abstract

摘要： 以银条‘二细一粗’品种为试材, 研究了蔗糖浓度、激素组合对茎尖培养和快速繁殖的影响
及温度、光照、蔗糖浓度等因素对根状茎离体诱导的影响。结果表明: 茎尖培养较理想的培养基为MS + 蔗糖4 % + 6-BA 0.5 mg·L^{- 1} + NAA 0.1 mg·L ^{- 1} , 成苗率可达74.0 %。茎尖增殖较适培养基为MS + 6-BA 3.0 mg·L^{- 1} + NAA 0.5 mg·L ^{- 1} , 每个外植体平均可产生5.6 个芽。根状茎诱导以MS + 蔗糖10 % + 6-BA 510 mg·L^{- 1}+ CCC 500 mg·L^{- 1}培养基, 20 ℃全黑暗培养效果最好。

关键词: 银条, 茎尖培养, 快速繁殖, 根状茎诱导

Abstract: The factors which affect tip meristem culture , rapid propagation and the induction of in vitro stolon of manyflower Betony ( Stachys floridana Schuttl . ex Benth. ) were studied. The results showed that the optimum medium for tip meristem culture were MS + sucrose 4 % + 6-BA 0.5mg·L^{- 1} + NAA 0.1 mg·L ^{- 1} with 74.0 %plant let ratio. MS supplemented with 6-BA 3.0 mg·L ^{- 1} and NAA 0.5 mg·L ^{- 1} was successful for propagation , each explant produced up to 5.6 seedlings. Total darkness , 20 ℃and MS medium with 6-BA 5.0 mg·L^{- 1} + CCC 500 mg·L^{- 1} were better for the induction of in vitro stolon.

Key words: Manyflower Betony ( Stachys floridana Schuttl . ex Benth. ), Tip meristem culture, Propagation, Stolon induction

中图分类号:

S 632.9

张国裕;程智慧;李娟;周文安;王新华. 银条茎尖培养快繁及离体根状茎的诱导[J]. 园艺学报, 2004, 31(1): 106-108.

Zhang Guoyu;Cheng Zhihui;Li Juan;Zhou Wenpan;Wang Xinhua. Studies on Tip Meristem Culture Rapid Propagation and Induction of in VitroStolon of Manyflower Betony ( Stachys floridana Schuttl . ex Benth. )[J]. ACTA HORTICULTURAE SINICA, 2004, 31(1): 106-108.

[1]	刘剑锋;程云清;陈智文;端爱萍. 平欧杂交榛组织培养与快速繁殖技术研究[J]. 园艺学报, 2009, 36(3): 409-414.
[2]	宋尚伟;苗红霞;胡青霞;王娟. 银条茎尖玻璃化法超低温保存及其植株再生[J]. 园艺学报, 2009, 36(12): 1810-1815.
[3]	李海云;王中伟;马瑞;邢少辰;刘艳芝;董英山. 山楂海棠的离体培养与快速繁殖[J]. 园艺学报, 2008, 35(5): 660-660.
[4]	其其格;;李双福;张启昌;张英楠. 西伯利亚白刺的丛生芽诱导及快速繁殖[J]. 园艺学报, 2007, 34(3): 791-792.
[5]	顾福根;万志刚;孙丙耀;赵林川. 夏蜡梅的离体培养和快速繁殖[J]. 园艺学报, 2007, 34(2): 324-324.
[6]	郭东伟;;马有志;周一龙;闵东红;陈耀锋. 银条根茎离体诱导[J]. 园艺学报, 2006, 33(4): 873-875.
[7]	魏琪;李凤兰;胡国富;胡宝忠. 蝴蝶兰快速繁殖研究进展[J]. 园艺学报, 2006, 33(4): 915-920.
[8]	宋会访;葛　红;周　媛;王彩云. 桂花离体培养与快速繁殖技术的初步研究[J]. 园艺学报, 2005, 32(4): 738-740.
[9]	刘奕清　王大平　熊运海. 尾巨桉的离体培养和快速繁殖[J]. 园艺学报, 2005, 32(4): 672-672.
[10]	张明丽;李　青. 金叶红瑞木的组织培养与快速繁殖[J]. 园艺学报, 2005, 32(4): 728-728.
[11]	王艳军;;李锡香;向长萍;沈　镝;宋江萍. 大蒜茎尖玻璃化法超低温保存技术研究[J]. 园艺学报, 2005, 32(3): 507-509.
[12]	江洪如;余发新;刘腾云. 红千层的离体培养及快速繁殖[J]. 园艺学报, 2005, 32(3): 541-543.
[13]	钱剑林;朱旭东;;田松青;尤伟忠;毛安元 . 东方百合‘Sorbonne’试管成球和低温处理的初步探讨[J]. 园艺学报, 2004, 31(6): 828-828.
[14]	陈典;黄晓梅;姜玉东;梁艳. 大葱茎尖脱毒苗培养及增殖技术的研究[J]. 园艺学报, 2004, 31(5): 673-675.
[15]	姜秀芳;张改英;田炜;王凤真. 芋茎尖分生组织培养的研究[J]. 园艺学报, 2004, 31(4): 466-466.