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园艺学报 ›› 2004, Vol. 31 ›› Issue (1): 99-100.

• 研究报告 • 上一篇    下一篇

高温胁迫对菠菜叶片保护酶活性和膜透性的影响

李敏;王维华;王然;刘润进   

  1. (莱阳农学院菌根实验室, 莱阳265200)
  • 收稿日期:2003-01-27 修回日期:2003-04-07 出版日期:2004-02-25 发布日期:2004-02-25

Influences of High Temperature Stress on Activity of Cell Defense Enzymes andMembrane Permeability in Leaves of Spinach

Li Min;Wang Weihuan;Wang Ran;Liu Runjin   

  1. ( Mycorrhiza Laboratory , Laiyang Agricultural University , Laiyang 265200 , China)
  • Received:2003-01-27 Revised:2003-04-07 Online:2004-02-25 Published:2004-02-25

摘要: 对两个耐热性不同的菠菜( Spinacia oleracea L. ) 品种进行了高温(昼35 ℃/ 夜25 ℃) 处理, 以20 ℃/ 15 ℃为对照, 测定了高温胁迫对菠菜叶片中保护性酶类活性和膜透性的影响。结果表明, 高温处理后, 耐热品种叶片中超氧化物歧化酶(SOD) 、过氧化氢酶(CAT) 和过氧化物酶(POD) 活性的增幅大于不耐热品种, 而不耐热品种叶片中丙二醛(MDA) 含量和膜透性的增幅大于耐热品种。

关键词: 菠菜, 高温胁迫, 保护酶, 丙二醛, 膜透性

Abstract: Seedlings of two spinach ( Spinacia oleracea L. ) cultivars were treated with high temperature (day 35 ℃/ night 25 ℃) . The activity of defense enzymes and the cell membrane permeability in leaves of spinach were determined. The results showed that after being treated with high temperature , the increasing range of the superoxide (SOD) , catalase (CAT) and peroxidase (POD) activity in cultivar tolerant to high temperature was greater than that of cultivar non2tolerant to high temperature. The increasing scope of malondialdehyde (MDA) contents and the cell membrane permeability in cultivar non-tolerant to high temperature was greater than in cultivar tolerant to high temperature.

Key words: Spinacia oleracea L., High temperature stress, Defense enzymes, Malondialdehyde, Membrane permeability

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