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园艺学报 ›› 2004, Vol. 31 ›› Issue (1): 78-80.

• 研究报告 • 上一篇    下一篇

枣的胚败育及幼胚培养研究

祁业凤;刘孟军*

  

  1. (河北农业大学中国枣研究中心, 保定071001)
  • 收稿日期:2003-01-28 修回日期:2003-04-30 出版日期:2004-02-25 发布日期:2004-02-25
  • 通讯作者: 刘孟军

Embryo Abortion and Young Embryo Culture of Chinese Jujube

Qi Yefeng ; Liu Mengjun*   

  1. ( Research Center of Chinese Jujube , Agricultural University of Hebei , Baoding 071001 , China)
  • Received:2003-01-28 Revised:2003-04-30 Online:2004-02-25 Published:2004-02-25
  • Contact: Liu Mengjun

摘要: 对30 个枣优良品种坐果后30 d 的胚败育情况进行了调查, 结果表明, 败育率高于75 %的高度
败育品种占53.4 % , 败育率介于25 %至75 %的中度败育品种占3.3 % , 败育率低于25 %的轻度败育品种占43.3 %。以优良品种‘冬枣’、‘金丝丰’等为试材, 对坐果后10~70 d 幼胚进行培养的结果表明, 枣离体胚接种后主要有3 种生长方式, 即形成愈伤组织、子叶间小叶增生和胚直接萌发成苗。本文还提出了提高枣胚培养及胚培养苗移栽成功的技术要点。

关键词: 枣, 胚, 败育, 组织培养

Abstract: The embryo abortion rate of 30 excellent cultivars of Chinese jujube ( Zizyphus jujuba Mill . ) were investigated 30 days after fruit set . The results showed that , the high abortion cultivars with more than 75 % aborted embryo , the middle abortion cultivars with 25 % - 75 % aborted embryo and the light abortion cultivars with less than 25 % aborted embryo made up 53.4 % , 3.3 % and 43.3 %of the cultivars studied respectively. 10 - 70 days old embryos of Z. jujuba‘Dongzao’and‘Jinsifeng’etc were employed to study embryo culture system. There were 3 growth-styles of the young embryos after inoculated , i . e. , forming callus , multiplicating small leaf in the center of cotyledons and forming seedling directly. The measures improving embryo culture and transplant of seedling were also studied.

Key words: Chinese jujube, Embryo, Abortion, Tissue culture

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