关键词: 花烛, 愈伤组织, 再分化, 生理生化特性, 内源激素

Abstract: Calli of Anthurium andraeanum‘Jolanba’derived from leaves of in vitro plantlets were cultured with three different treatments to observe their redifferentiation behaviors , and their physiological and biochemical features are discussed. The results went as followed. Decrease of base medium concentration , reduction of sub-culture frequency , or prolongation of sub-culture period favored differentiation and growth of regenerated plantlets.High content of soluble protein favored shoot differentiation , and high content of soluble sugar enhanced shoot growth. There was obvious positive correlation between activities of POD and SOD and number of differentiated shoots. So was between GA_{1 + 3}/ ABA , as well as ZR_s/ ABA , and shoot height . The IAA/ ABA ratio or IAA was also related to rhizogenesis. One of the physiological and biochemical causes for the enhancement growth vigor of aseptic plantlet by renewing in vitro culture system and prolonging culture period was maintaining high content of endogenous soluble sugar and favorable balance of endogenous phytohormes.

Key words: Anthurium andraeanum, Redifferentiation, Physiological and biochemical features, Endogenous phytohormones