柿树乙醇脱氢酶活性与可溶性单宁含量的关系

园艺学报 ›› 2008, Vol. 35 ›› Issue (5): 741-746.

柿树乙醇脱氢酶活性与可溶性单宁含量的关系

刘朝蓬¹;郑仲明²;梁银娜¹;李宝^1*

(¹中国农业大学农学与生物技术学院, 北京100094；²北京市房山区林业局林果站, 北京102400 )

收稿日期:2008-03-24 修回日期:2008-04-29 出版日期:2008-05-25 发布日期:2008-05-25
通讯作者: 李宝

Relationship Between Activity of Alcohol Dehydrogenase and Soluble Tannin in Persimmon

LIU Chao-peng¹, ZHENG Zhong-ming², LIANG Yin-na¹, and LI Bao^1*

(¹ College of Agriculture and Biotechnology, China Agricultural University, Beijing 100094, China；²Forestry & Fruit Station of Forestry Bureau, Fangshan District of Beijing Municipality, Beijing 102400, China )

Received:2008-03-24 Revised:2008-04-29 Online:2008-05-25 Published:2008-05-25
Contact: LI Bao

摘要/Abstract

摘要：

研究了4种不同脱涩类型柿(Diospyros kaki Thunb)果实和叶片中的可溶性单宁含量、乙醇脱氢酶（alcohol dehydrogenase, ADH）活性和ADH最适反应温度，以了解ADH在脱涩过程中的作用。结果表明：不完全甜柿和完全甜柿果实发育前期，可溶性单宁含量逐渐降低，ADH活性逐渐增高，到果实基本完成脱涩后，ADH活性又开始降低，在不完全甜柿中变化较大；涩柿和不完全涩柿在果实近熟期，可溶性单宁含量有所下降，但不能完成脱涩，且ADH活性较低。叶片发育过程中，可溶性单宁含量的变化与ADH没有相关性。乙醇脱氢酶的最适反应温度在25℃左右。ADH与柿果实脱涩有一定关系。

关键词: 柿, 可溶性单宁, 乙醇脱氢酶, 脱涩

Abstract:

Content of soluble tannin and activity of alcohol dehydrogenase and the optimum temperature of alcohol dehydrogenase (ADH) in 4 different type fruits of persimmon (Diospyros kaki Thunb) were measured, in order to assess the effects of ADH on loss of astringency of persimmon fruits. In the early stage of fruits development, the pollination constant non-astringent (PCNA) and the pollination variant non-astringent type (PVNA) soluble tannin content decreased, and the activity of ADH increased, but the activity of ADH decreased when the fruits become non-astringent, especially in PVNA-type fruits. The content of soluble tannin of pollination constant astringent (PCA) and pollination variant astringent (PVA) -type fruits decreased, and the activity of ADH was also in the lower level in the mature stage. During the leaf development, there is no significant relationship between ADH and soluble tannin. The optimum temperature of ADH is at 25℃. ADH plays a role to some extent in loss of astringency of persimmon fruits.

Key words: Diospyros kaki Thunb, soluble tannin, alcohol dehydrogenase, deastringency

中图分类号:

S 665.2

刘朝蓬;郑仲明;梁银娜;李宝. 柿树乙醇脱氢酶活性与可溶性单宁含量的关系[J]. 园艺学报, 2008, 35(5): 741-746.

LIU Chao-peng;ZHENG Zhong-ming;LIANG Yin-na;and LI Bao. Relationship Between Activity of Alcohol Dehydrogenase and Soluble Tannin in Persimmon[J]. ACTA HORTICULTURAE SINICA, 2008, 35(5): 741-746.

[1]	张悦, 索玉静, 孙鹏, 韩卫娟, 刁松锋, 李华威, 张嘉嘉, 傅建敏, 李芳东. 柿种质资源果实形态多样性分析[J]. 园艺学报, 2022, 49(7): 1473-1490.
[2]	杨思潮, 张萌, 张青林, 罗正荣. 中国甜柿自然脱涩特点及其机理研究进展[J]. 园艺学报, 2022, 49(12): 2659-2668.
[3]	李华威1，王丽媛2，孙鹏2，索玉静2，韩卫娟2，买旖旎2，刁松锋2，袁德义1,，傅建敏2,. ‘龙岩野柿1号’雄花和两性花雌雄蕊发育的细胞形态学观察[J]. 园艺学报, 2019, 46(10): 1897-1906.
[4]	刁松锋，韩卫娟，李芳东，孙鹏，索玉静，傅建敏*. 柿新品种‘中柿2号’[J]. 园艺学报, 2018, 45(5): 1009-1010.
[5]	王丽媛，孙鹏，张嘉嘉，傅建敏*，刁松锋，韩卫娟，索玉静，张悦. 柿野生雄性资源调查及其遗传多样性研究[J]. 园艺学报, 2018, 45(2): 261-278.
[6]	刁松锋1，李芳东1，梁臣2，孙鹏1，韩卫娟1，索玉静1，傅建敏1,*. 柿新品种‘中柿1号’[J]. 园艺学报, 2017, 44(s2): 2661-2662.
[7]	徐君驰1，张青林1,2，徐莉清1,2，郭大勇2，罗正荣2,*. 柿果脱涩机理研究新进展[J]. 园艺学报, 2016, 43(9): 1653-1664.
[8]	张永芳，胡超琼，杨勇*，朱仁胜，郭静，王仁梓 . 柿属8种植物花粉形态观察[J]. 园艺学报, 2016, 43(6): 1167-1174.
[9]	李加茹，孙鹏，韩卫娟，李芳东，傅建敏*，刁松锋. 不完全甜柿‘禅寺丸’花性别分化形态学关键时期的研究[J]. 园艺学报, 2016, 43(3): 451-461.
[10]	张娜1，莫荣利1,2，张青林1,3，罗正荣1,3,*. 完全雄性柿种质‘雄株8 号’作为中国甜柿育种亲本的可能性研究[J]. 园艺学报, 2016, 43(11): 2133-2140.
[11]	张平贤1，何欢1，罗正荣1,3，杨勇2，王仁梓2，张青林1,3,*. DlSx-AF4S标记在柿及其杂交后代性别鉴定中的有效性研究[J]. 园艺学报, 2016, 43(1): 47-54.
[12]	孙振营韩叶李秀芳马秋诗饶景萍. 柿采后丙烯和1–甲基环丙烯处理对两个扩展蛋白基因表达的影响[J]. 园艺学报, 2014, 41(6): 1089-1095.
[13]	梁晋军, 梁玉琴, 傅建敏. 基于叶绿体DNA PCR-RFLP分析柿及其近缘种亲缘关系[J]. 园艺学报, 2014, 41(12): 2474-2480.
[14]	陈小云, 张仕杰, 屠煦童, 李宁宁, 章镇, 渠慎春. ‘南通小方柿’乙醇脱氢酶基因DkADH1的克隆及表达分析[J]. 园艺学报, 2014, 41(10): 2001-2011.
[15]	祝庆刚, 饶景萍, 田红炎, 韩叶. 丙烯和1–甲基环丙烯处理对采后柿果实XTH基因表达的影响[J]. 园艺学报, 2012, 39(7): 1278-.