红叶石楠生根培养与根系活力的研究

园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 1129-1132.

红叶石楠生根培养与根系活力的研究

李际红¹;韩小娇²;卢胜西¹;孙仲序²

(¹ 山东农业大学科技学院, 山东泰安271000; ²山东农业大学园艺科学与工程学院, 山东泰安271018)

收稿日期:2005-11-01 修回日期:2006-03-21 出版日期:2006-10-25 发布日期:2006-10-25

The Study of Root Induction and Root Vigor in Photinia fraseri in Vitro

Li Jihong¹;Han Xiaojiao²;Lu Shengxi¹;Sun Zhongxu²

(1College of Science and Technology, Shandong Agricultural University, Taipan, Shandong 271000, China; 2College of Horti2culture Science and Engineering, Shandong Agricultural University, Taipan, Shandong 271018, China)

Received:2005-11-01 Revised:2006-03-21 Online:2006-10-25 Published:2006-10-25

摘要/Abstract

摘要： 以红叶石楠继代组培苗为试材, 研究了不同生长素( IAA、NAA和IBA) 、间苯三酚、暗培养、多效唑(MET) 以及培养基支持体对红叶石楠生根过程的影响。结果表明: NAA 015 mg·L^{- 1}和暗培养7 d促进红叶石楠的生根; 培养基1/2MS +NAA 0.5 mg·L ^{- 1} + IBA 0.3 mg·L^{- 1} +MET 0.5 mg·L ^{- 1}的处理使平均每株生根数达615条, 生根率达到90%; 以琼脂、蛭石、珍珠岩为培养基支持体诱导生根, 其中以珍珠岩为培养基支持体诱导效果最佳, 平均每株生根数达2318条, 生根率达96.6% , 移栽成活率达95.9% , 根系活力高达576.159μg·g^{- 1} ·h^{- 1} , 过氧化物酶( POD) 活性达到27.08 △A₄₇₀ ·min^{- 1} ·g^{- 1}。

关键词: 红叶石楠, 生根培养, 培养基支持体, 根系活力, POD活性

Abstract: The effects of different auxin ( IAA, NAA and IBA) , phloroglucinol, culture in dark, paclobutrazol (MET) and supportingmaterials on Photinia fraseri rooting were studied. The results showed that NAA 0.5 mg·L^{- 1} and culture in dark for 7 days promoted rooting through orthogonal experiment; and when the 1 /2MS +NAA 0.5 mg·L^{- 1} + IBA 0.3 mg·L ^{- 1} +MET 0.5 mg·L^{- 1} medium induced rooting, mean number of roots was 6.5, rooting rate was 90%. Besides, the effects of agar, veimiculite and pearlite as supporting materials were studied. Pearlite was the best supporting material; mean number of roots was 23.8; rooting rate was 9616%; the survive rate of transplant seedlings reached 95.9%; root vigor reached 576.159μg·g^{- 1} ·h^{- 1} ; POD activity reached 27.08 △A₄₇₀ ·min^{- 1} ·g^{- 1}.

Key words: Photinia fraseri, Rooting culture, Supporting material, Root vigor, POD activity

李际红;韩小娇;卢胜西;孙仲序. 红叶石楠生根培养与根系活力的研究[J]. 园艺学报, 2006, 33(5): 1129-1132.

Li Jihong;Han Xiaojiao;Lu Shengxi;Sun Zhongxu. The Study of Root Induction and Root Vigor in Photinia fraseri in Vitro[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 1129-1132.

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