水杨酸对根际低氧胁迫八棱海棠幼苗活性氧代谢的影响

园艺学报 ›› 2008, Vol. 35 ›› Issue (2): 163-168.

水杨酸对根际低氧胁迫八棱海棠幼苗活性氧代谢的影响

白团辉¹;马锋旺^{1,2<SPAN lang=EN-US style=}

(¹西北农林科技大学园艺学院，陕西杨凌 712100；²山东农业大学园艺科学与工程学院，山东泰安 271000)

收稿日期:2007-09-28 修回日期:2008-01-10 出版日期:2008-02-25 发布日期:2008-02-25
通讯作者: 马锋旺

Effects of Salicylic Acid on Reactive Oxygen Species Metabolism in Malus robusta Rehd. under Root-zone Hypoxia Stress

BAI Tuan-hui¹,MA Feng-wang^{1,2<SPAN lang=EN-US style=}

(¹College of Horticulture, Northwest A ＆ F University, Yangling, Shaanxi 712100, China; ²College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271000, China)

Received:2007-09-28 Revised:2008-01-10 Online:2008-02-25 Published:2008-02-25
Contact: MA Feng-wang

摘要/Abstract

摘要： 采用营养液水培系统，以苹果砧木八棱海棠(Malus robusta Rehd.)为试材，用叶面喷施的方法，研究了外源水杨酸(salicylic acid ,SA)对根际低氧胁迫下八棱海棠叶片活性氧(ROS)代谢的影响。结果表明：低氧胁迫下八棱海棠叶片丙二醛(MDA)、超氧阴离子 (O₂)、过氧化氢(H₂O₂)、谷胱甘肽(GSH)含量和抗氧化酶活性均高于对照，而抗坏血酸(AsA)含量低于对照；外源SA明显抑制MDA和O₂的积累，显著增强超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)的活性。说明SA作为化学诱抗剂，可抑制低氧胁迫下苹果幼苗体内活ROS的产生，提高抗氧化酶的活性，降低膜脂过氧化水平，从而减轻低氧胁迫对植株的伤害。

关键词: 水杨酸, 八棱海棠, 低氧胁迫, 抗氧化酶, 活性氧

Abstract: By means of a system of nutrient solution culture, an apple rootstock, Malus robusta Rehd. was used to study effects of foliar application of exogenous salicylic acid (SA) on reactive oxygen species metabolism under root-zone hypoxia stress. The results showed that the content of malonaldehyde(MDA), superoxide radical(O2), hydrogen peroxide(H2O2)and glutathione (GSH) and activities of antioxidant enzymes under root-zone hypoxia stress were higher than the control, however, the level of ascorbic acid (AsA) were decreased. Exogenous SA significantly decreased the content of O2, MDA and GSH, increased the activities of superoxide dismutase(SOD), peroxidase(POD), ascorbate peroxidase(APX)and glutathione reductase (GR)under hypoxia stress. It was proposed that exogenous SA as a chemical activator could induce resistance, reduce production of reactive oxygen species, raise the activities of anti-oxidative enzymes, and decrease the membrane lipid peroxidation, therefore it alleviated the damage degree of hypoxia stress of Malus robusta Rehd.

Key words: salicylic acid, Malus robusta Rehd., hypoxia stress, antioxidant enzymes

中图分类号:

S 661

白团辉;马锋旺;<SPAN lang=EN-US style=. 水杨酸对根际低氧胁迫八棱海棠幼苗活性氧代谢的影响[J]. 园艺学报, 2008, 35(2): 163-168.

BAI Tuan-hui;MA Feng-wang;<SPAN lang=EN-US style=. Effects of Salicylic Acid on Reactive Oxygen Species Metabolism in Malus robusta Rehd. under Root-zone Hypoxia Stress[J]. ACTA HORTICULTURAE SINICA, 2008, 35(2): 163-168.

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