芥蓝游离小孢子培养初报

园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 239-240.

芥蓝游离小孢子培养初报

何杭军;王晓武;汪炳良

( 中国农业科学院蔬菜花卉研究所，北京100081；浙江大学农业与生物技术学院园艺系，杭州310029)

收稿日期:2003-04-08 修回日期:2003-06-12 出版日期:2004-04-25 发布日期:2004-04-25

Embryogenesis and Plant Regeneration of Chinese Kale via Isolated Microspore Culture

He Hangjun;Wang Xiaowu;Wang Bingliang

( Institute of Vegetables and Flowers，Chinese Academy of Agricuhural Science，Beijing 100081，China； Department of Horticulture，Zhejiang University，Hangzhou 310029，China )

Received:2003-04-08 Revised:2003-06-12 Online:2004-04-25 Published:2004-04-25

摘要/Abstract

摘要： 以芥蓝的5个基因型为试材，对游离小孢子培养诱导胚状体发生及植株再生进行了初步探讨。
结果表明：供体基因型对成功诱导胚状体发生至关重要；振荡培养明显有利于胚状体的发育；改良MS和B培养基对诱导植株再生没有差别。

关键词: 芥蓝, 游离小孢子, 胚状体, 振荡培养, 再生植株

Abstract: Isolated microspores of 5 Chinese kale genotypes were cultured． It was found that only those late-uninucleate and binucleate microspores could grow into division． The results also showed that genotypes had important impact on embryoid induction． Effect of shaking culture on embryoids development was examined． The results demonstrated that slow shaking could promote development of embryoids and increase the proportion of cotyledonary embryoids．MS and B5 media showed no difference on plant
regeneration．

Key words: Chinese kale, Isolated mierospore, Embryoid, Shaking culture, Plant regeneration

中图分类号:

S 635

何杭军;王晓武;汪炳良. 芥蓝游离小孢子培养初报[J]. 园艺学报, 2004, 31(2): 239-240.

He Hangjun;Wang Xiaowu;Wang Bingliang. Embryogenesis and Plant Regeneration of Chinese Kale via Isolated Microspore Culture[J]. ACTA HORTICULTURAE SINICA, 2004, 31(2): 239-240.

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