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园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 233-235.

• 研究报告 • 上一篇    下一篇

茄子体细胞杂种游离小孢子培养获得再生植株

连 勇;刘富中;陈钰辉;宋 燕;张松林;Darasinh Sihachakr
  

  1. ( 中国农业科学院蔬菜花卉研究所,北京100081; 法国巴黎11大学植物形态与发育实验室,法国ORSAY CEDEXBAT.360,91405)
  • 收稿日期:2003-09-12 修回日期:2003-12-24 出版日期:2004-04-25 发布日期:2004-04-25

Plantlet Regeneration by Isolated Microspore Culture of Somatic Hybrid ofEggplant

Lian Yong ;Liu Fuzhong;Chen Yuhui;Song Yan;Zhang Songlin ; Darasinh Sihachakr
  

  1. ( Institute o fVegetabales and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China; Morphogenese Vegetable Experimentale,Universite Paris Sud,BAT.360,91405 ORSAY CEDEX,France)
  • Received:2003-09-12 Revised:2003-12-24 Online:2004-04-25 Published:2004-04-25

摘要:

应用游离小孢子培养技术,培养茄子体细胞融合杂种DSa不同株系的小孢子。结果表明:对
花药进行36 ℃高温6 d预处理,能显著提高小孢子脱分化能力;小孢子离体培养的最佳发育时期是单核期;小孢子培养的适宜培养基为KM附加PEG 250 mg/L、2,4-D 0.2 mg/L、ZT 0.5 mg/L、NAA 1 mg/L及6.5%的葡萄糖;再生植株适宜培养基为Ms附加ZT 2 mg/L、IAA 0.1 mg/L、2%蔗糖和7 g/L琼脂。

关键词: 茄子, 体细胞杂种, 小孢子培养, 植株再生

Abstract: Somatic fusion hybrid DSa(S.torvum Sw×S.melongena L.‘Dourga’)microspore were cultured by using isolated microspore culture. The result show that microspore dediferentiation ability was markedly improved by using the treatment of anther under the high temperature conditions of 36 ℃ for
continuous 6 days. The best development period for microspore culture is monokaryotie stage;The suitable medium for microspore culture is KM with PEG 250 mg/L,2,4-D 0.2 mg/L,ZT 0.5 mg/L,NAA 1 mg/L and glucose 6.5%;The suitable medium for regenerated plantlet is MS with ZT 2 mg/L,IAA 0.1 mg/L,sugar 2% and agar 7 g/L.

Key words: Eggplant, Somatic hybrid, Isolated microspore culture, Plantlet regeneration

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