甜樱桃砧木吉塞拉(Gisela)叶片再生体系研究

园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 221-223.

甜樱桃砧木吉塞拉(Gisela)叶片再生体系研究

黄文江;刘庆忠;樊圣华;赵红军;马锋旺

( 山东省果树研究所，泰安 271000；西北农林科技大学园艺学院，杨凌 712100)

收稿日期:2003-06-30 修回日期:2003-08-12 出版日期:2004-04-25 发布日期:2004-04-25

Studies on System of Plant Regeneration from Leaf Explant of Cherry Rootstocks

Huang Wenjiang ;Liu Qingzhong ;Fan Shenghua ;Zhao Hongjun; Ma Fengwang

( Shandong Institute of Pomology，Tai'an 271000，China； College of Horticulture，Northwestern Science and Technology University of Agriculture and Forestry，Yangling 712100，China)

Received:2003-06-30 Revised:2003-08-12 Online:2004-04-25 Published:2004-04-25

摘要/Abstract

摘要： 以甜樱桃矮化砧木‘Gisela 5’和‘Gisela 6’的组培苗为试材，研究建立叶片的离体再生体系。结果表明，Gisela 6的再生能力高于Gisela 5；叶片正面接触培养基比背面接触培养基更有利于分化再生。用新梢顶端1~3节新展开的叶片制备外植体，分别接种在WPM+BA 7 mg/L+IBA 0.3 mg/L和WPM+BA 5 mg/L+IBA 0.5 mg/L的培养基上，黑暗处理15 d，Gisela 5的最高分化频率为75.1%，Gisela 6为100%。再生芽苗在三角瓶中生根容易，移人大田后生长正常。

关键词: 樱桃, 砧木, 矮化, 离体叶片, 再生体系, 组织培养

Abstract: An efficient and simple method for shoots regeneration from in vitro leaves of ‘Gisela 5’ and ‘Gisela 6’ (Pruns cerasus×P．canescens)was developed．Factors influencing in vitro leaf regeneration were studied．The ability to regenerate shoot from in vitro leaves of‘Gisela 6’ was much greater than that of ‘Gisela 5’：Optimal results were obtained by culturing leaves adaxial side down on WPM medium containing BA 5 mg/L+IBA 0.5 mg/L and BA 7 mg/L+IBA 0.3 mg/L respectively in the dark for 15 days．Shoot regeneration rate of ‘Gisela 6’ reached up to 10o％ and that of ‘Gisela 5’ was 75.1% ．The regeneration shoot was easy to rooting and to transplant to the field．

Key words: Cherry, Rootstock, Dwarf, In vitro leaf, Regeneration, Tissue culture

中图分类号:

S663．1

黄文江;刘庆忠;樊圣华;赵红军;马锋旺. 甜樱桃砧木吉塞拉(Gisela)叶片再生体系研究[J]. 园艺学报, 2004, 31(2): 221-223.

Huang Wenjiang;Liu Qingzhong;Fan Shenghua;Zhao Hongjun;Ma Fengwang. Studies on System of Plant Regeneration from Leaf Explant of Cherry Rootstocks[J]. ACTA HORTICULTURAE SINICA, 2004, 31(2): 221-223.

[1]	吴延军, 刘庆忠, 陈鸿才, 戚行江, 朱东姿, 郑家祥, 曹学敏, 方丹燕. 甜樱桃新品种‘江南锦’[J]. 园艺学报, 2022, 49(S2): 29-30.
[2]	张晓明, 闫国华, 周宇, 王晶, 段续伟, 吴传宝, 张开春. 甜樱桃砧木新品种‘京春2号’[J]. 园艺学报, 2022, 49(S2): 31-32.
[3]	郑积荣, 王同林. 番茄新品种‘杭杂601’[J]. 园艺学报, 2022, 49(S2): 105-106.
[4]	郑积荣, 王同林. 樱桃番茄新品种‘杭杂503’[J]. 园艺学报, 2022, 49(S2): 107-108.
[5]	田红梅, 刘娟, 张长坤, 陶珍, 张建, 王朋成, . 甜瓜砧木用南瓜新品种‘皖砧6号’[J]. 园艺学报, 2022, 49(S2): 127-128.
[6]	柏文富, 禹霖, 聂东伶, 李建挥, 熊颖, 严佳文, 李柏海, 吴思政. 樱花新品种‘湘韵’[J]. 园艺学报, 2022, 49(S2): 247-248.
[7]	梁荣, 李振权, 叶小玲, 胡晓敏, 朱军, 李园凤. 樱花新品种‘醉红颜’[J]. 园艺学报, 2022, 49(S2): 251-252.
[8]	张伟, 胡晓敏, 叶小玲, 朱军, 高珊, 陈端妮. 樱花新品种‘合家欢’[J]. 园艺学报, 2022, 49(S2): 255-256.
[9]	张伟, 胡晓敏, 叶小玲, 朱军, 冯钦钊, 杨梓滨. 樱花新品种‘小桃红’[J]. 园艺学报, 2022, 49(S2): 257-258.
[10]	王燕, 刘针杉, 张静, 杨鹏飞, 马蓝, 王旨意, 涂红霞, 杨绍凤, 王浩, 陈涛, 王小蓉. 中国樱桃杂交F₁代花和果实若干性状遗传倾向分析[J]. 园艺学报, 2022, 49(9): 1853-1865.
[11]	聂鑫淼, 栾恒, 冯改利, 王超, 李岩, 魏珉. 硅营养和嫁接砧木对黄瓜幼苗耐冷性的影响[J]. 园艺学报, 2022, 49(8): 1795-1804.
[12]	郑晓东, 袭祥利, 李玉琪, 孙志娟, 马长青, 韩明三, 李少旋, 田义轲, 王彩虹. 油菜素内酯对盐碱胁迫下平邑甜茶幼苗生长的影响及调控机理研究[J]. 园艺学报, 2022, 49(7): 1401-1414.
[13]	冯琛, 黄学旺, 李兴亮, 周佳, 李天红. 不同苹果矮化砧穗组合的抗旱性比较研究[J]. 园艺学报, 2022, 49(5): 945-957.
[14]	刘众杰, 郑婷, 赵方贵, 傅伟红, 诸葛雅贤, 张志昌, 房经贵. 葡萄砧木对渗透胁迫的抗性差异及生理响应机理[J]. 园艺学报, 2022, 49(5): 984-994.
[15]	李丽仙, 王烁, 陈莹, 邬滢涛, 王雅倩, 房月, 陈学森, 田长平, 冯守千. 甜樱桃PavMYB10.1促进PavRiant表达和花青苷积累[J]. 园艺学报, 2022, 49(5): 1023-1030.