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园艺学报 ›› 2009, Vol. 36 ›› Issue (10): 1498-1503.

• 观赏植物 • 上一篇    下一篇

利用早园竹叶绿体基因序列分析其在禾本科植物中的分类地位

李潞滨1;唐征2;庄彩云1,2;胡陶1,2;姚娜1,2;杨凯2*   

  1. (1中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京100091; 2北京农学院农业应用新技术北京市重点实验室, 北京102206)
  • 收稿日期:2009-12-24 修回日期:2009-09-07 出版日期:2009-10-25 发布日期:2009-10-25
  • 通讯作者: 杨凯

Taxonomic Status of Phyllostachys propinqua McClure in Poaceae Basing onSequence Analysis of Chloropla st Genes

LI Lu-bin1;TANG Zheng2;ZHUANG Cai-yun1,2;HU Tao1,2;YAO Na1,2;YANG Kai2*   

  1. (1 Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry,Beijing 100091, China; 2 Key Laboratory of Agricultural New Technology and Application in Beijing, Beijing Agricultural College, Beijing 102206, China)
  • Received:2009-12-24 Revised:2009-09-07 Online:2009-10-25 Published:2009-10-25
  • Contact: YANG Kai

摘要: 从构建的早园竹( Phyllostachys propinqua McClure) 叶绿体DNA基因组文库中克隆了早园竹叶绿体atpA (EU64861) 、psaB ( EU64863) 、rpoA ( EU64862) 和rpoC1 EU64864) 4个基因, 并将其序列分别与GenBank中部分禾本科植物的基因序列进行了比对分析, 构建核苷酸序列的Neighbor-joining系统进化树, 分析探讨其在禾本科中的分类地位。结果表明, 禾本科植物在系统分类上呈现出两大类群。第一大类包括两个亚类, 水稻与竹类植物聚为一亚类; 玉米、高粱和甘蔗聚为另一亚类。另一大类为小麦、大麦、匍茎剪股颖和黑麦草。表明早园竹与水稻分类地位最为接近; 比水稻和玉米、高粱与甘蔗更为较近, 与禾本科中麦类及其近缘植物关系较远。

关键词: 禾本科, 早园竹, 叶绿体, DNA序列, 系统分类

Abstract: Following of Phyllostachys propinqua McClure chlorop last genomic DNA library constructed,the atpA (EU64861) , psaB ( EU64863) , rpoA ( EU64862) and rpoC1 ( EU64864) genes were cloned and analyzed basing on sequence alignment with other chlorop last genes of Poaceae species in GenBank. In
order to analyze the taxonomic status of Phyllostachys propinqua in Poaceae, four phylogenetic treeswere constructed by neighbor-joining method. The results indicated that the gramineous plants can be classified as two classes in the phylogenetic systematics, the first class contained two subclasses, with Phyllostachys propinqua and rice together as one of the subclass and maize, sorghum, saccharum composing another; The second class included wheat, barley, ryegrass and creep ing bentgrass. It showed that Phyllostachys propinqua had the closest taxonomic status with rice, and it was much closer than that between rice and maize or sorghum and saccharum and the relation between Phyllostachys propinqua and wheat as well as itps kindred plant was
far

Key words: Poaceae, Phyllostachys propinqua McClure, chlorop last, DNA sequence, phylogenetic systematics

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