部分杧果品种亲缘关系的ISSR分析

园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 87-92.

部分杧果品种亲缘关系的ISSR分析

王家保^1*
;王令霞² ;杜中军¹ ; 雷新涛³ ;陈业渊⁴ ; 徐碧玉¹

(¹ 中国热带农业科学院热带生物技术研究所, 热带作物生物技术国家重点实验室, 海口571101; ² 华南热带农业大学园艺学院, 海南儋州571737; ³ 中国热带农业科学院南亚热带作物研究所, 广东湛江524091; ⁴ 中国热带农业科学院热带作物品种资源研究所, 海南儋州571737)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Analysis on the Genetic Relationship of Some Mango (Mangifera indica L. )Germplasms by ISSRMarkers

WANG J ia-bao^1*
;WANG Ling-xia² ; DU Zhong-jun¹ ;LE IXin-tao³ ; CHEN Ye-yuan⁴; XU B i-yu¹

(¹Institute of Tropical Biotechnology, State Key Laboratory of Biotechnology for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; ² College of Horticulture, South China University of Tropical Agricultural, Danzhou,Hainan 571737, China; ³ Institute of Southern Subtropical Crops, Chinese Academ y of Tropical Agricultural Sciences, Zhanjiang,Guangdong 524091, China; ⁴ Institute of Tropical Crop Germplasm, Chinese Academ y of Tropical Agricultural Sciences, Danzhou,Hainan 571737, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要/Abstract

摘要： ISSR技术分析了38个杧果(Mangifera indica L. ) 品种的亲缘关系。从60条ISSR引物中
筛选出10条重复性好、扩增带型清晰的引物, 共扩增出79条可重复性带, 多态性带比例为86.7%。扩增
结果可区分38个品种中的37个(含红金龙或贵妃之一) 。各品种间遗传距离在0～0.682之间, 平均为
0.393。UPGMA聚类将供试品种分成了5类。对分类结果进行了讨论。

关键词: 杧果, 亲缘关系, ISSR, 聚类分析

Abstract: The genetic relationship s of 38 mango (Mangifera indica L. ) accessions were examined by
ISSR markers. Of the 60 ISSR p rimers screened, only 10 p rimers gave rep roducible, polymorphic DNA ampli
fication patternswith a total 79 of amplified bands. Their percentage of polymorphic band was 86.7%. By
banding patterns obtained from these 10 primers, each accession could be distinguished from the others excep t
either Hongjinlong or Guifei, since the two accessions had the same amplified band patterns. Genetic distances
among the tested accessions ranged from 0 to 0.682 with an average of 0.393. Five group s were formed by
UPGMA clustering and the classification results were discussed in this paper.

Key words: Mango, Mangifera indica L., Genetic relationship, ISSR, Clustering analysis

王家保;王令霞;杜中军;雷新涛;陈业渊;徐碧玉. 部分杧果品种亲缘关系的ISSR分析[J]. 园艺学报, 2007, 34(1): 87-92.

WANG J ia-bao;WANG Ling-xia;DU Zhong-jun;LE IXin-tao;CHEN Ye-yuan;XU B i-yu. Analysis on the Genetic Relationship of Some Mango (Mangifera indica L. )Germplasms by ISSRMarkers[J]. ACTA HORTICULTURAE SINICA, 2007, 34(1): 87-92.

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