番茄转ER-sHSP基因植株构建及其抗冷性研究

园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 989-994.

番茄转ER-sHSP基因植株构建及其抗冷性研究

赵春梅;王丽;伊淑莹;刘箭^*

(山东师范大学生命科学学院, 山东济南250014)

收稿日期:2006-04-14 修回日期:2006-09-20 出版日期:2006-10-25 发布日期:2006-10-25

The Construction and the Chilling-Resistance Ability of Endoplasmic Reticulum Small Heat Shock Protein ( ER-sHSP) Transgenic Tomato Plants

Zhao Chunmei;Wang Li;Yi Shuying;Liu Jian^*

(College of Life Sciences, Shandong Normal University, Ji'nan, Shandong 250014, China)

Received:2006-04-14 Revised:2006-09-20 Online:2006-10-25 Published:2006-10-25

摘要/Abstract

摘要： 将CaMV 35S启动子驱动的内质网小分子热激蛋白基因导入番茄, 比较转基因、未转基因和转空载体番茄的抗冷能力。冷胁迫下, 同对照相比, 转基因番茄的冷害症状轻, 叶绿素含量的损失少, 体内积累的丙二醛(MDA) 含量少, 电解质外渗程度低, 具有较高的净光合速率和最大光化学效率, 并易于恢复由低温所引起的光抑制, 表明转基因番茄具有较强的冷胁迫耐性, 说明内质网小分子热激蛋白在植物抗冷过程中发挥重要作用。

关键词: 番茄, 内质网, 小分子热激蛋白, 转基因, 冷胁迫

Abstract: The full-length endoplasmic reticulum small heat shock protein ( ER-sHSP) cDNA using CaMV 35S p romoterwas transformed into the genome of tomato plants. The chilling tolerance of transgenic tomato plants, non2transgenic tomato plants and pROKⅡ-transformed tomato plants were studied. Under chilling stress treatment, compared with non-transgenic and pROKⅡ-transformed tomato plants, transgenic tomato plants exhibited slighter cold-injured symptoms, suffered less destruction of chlorophyll and electrolyte leakage. The content of MDA of transgenic tomato plants was lower than that of non-transgenic and pROKⅡ-transformed tomato plants, and transgenic tomato plants could keep up higher value of net photosynthetic rate, Fv/Fm and could recover quickly from chilling-induced photosynthetic inhibition. The results showed that ERsHSP played a key role in enhancing the chilling-resistance ability of plants.

Key words: Tomato, Endoplasmic reticulum, Small heat shock protein, Transgenic, Chilling stress

赵春梅;王丽;伊淑莹;刘箭. 番茄转ER-sHSP基因植株构建及其抗冷性研究[J]. 园艺学报, 2006, 33(5): 989-994.

Zhao Chunmei;Wang Li;Yi Shuying;Liu Jian. The Construction and the Chilling-Resistance Ability of Endoplasmic Reticulum Small Heat Shock Protein ( ER-sHSP) Transgenic Tomato Plants[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 989-994.

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