白菜病程相关蛋白1基因cDNA全长的克隆与分析

园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 985-988.

白菜病程相关蛋白1基因cDNA全长的克隆与分析

王彦华^{1, 2};侯喜林^1*;申书兴²

(¹南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京210095;² 河北农业大学园艺学院, 河北保定071001)

收稿日期:2006-02-21 修回日期:2006-08-25 出版日期:2006-10-25 发布日期:2006-10-25

Cloning and Characterization of a Full-length cDNA of Pathogenesis-relatedProtein 1 in Brassica rapa ssp. chinensis

Wang Yanhua^{1, 2};Hou Xilin^1*;Shen Shuxing²

(¹National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;²College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071001, China)

Received:2006-02-21 Revised:2006-08-25 Online:2006-10-25 Published:2006-10-25

摘要/Abstract

摘要： 以水杨酸处理后的‘苏州青’白菜为材料, 通过RT2PCR和RACE技术, 获得了白菜病程相关蛋白PR-1基因的cDNA全长序列。该基因与甘蓝型油菜PR-1基因氨基酸序列的同源性为91% , 与拟南芥的同源性为78% , 与其它植物的同源性为57%～60%。Southern杂交表明该基因在白菜基因组中的拷贝数至少为2个。半定量RT2PCR分析表明, 2 mmol/L的水杨酸处理后12 h, 该基因的表达量达到高峰。

关键词: 白菜, 病程相关蛋白1, 水杨酸, RT-PCR

Abstract: In this study, according to the consensus domain of the Brassica napus pathogenesis-related protein PR-1 gene ( accession number U21849) , using RT-PCR and RACE technology, a full-length cDNA of pathogenesis-related protein 1 named BcPR-1 was obtained from Brassica rapa ssp. chinensis cultivar
Suzhouqing, which displayed resistance to downy mildew. Sequence analysis indicated that cloned BcPR-1 gene consisted of 646 nucleotides (nt) containing 161 amino acids. Further comparison to B. napus PR-1 gene and Arabidopsis thaliana PR-1 gene showed that its identitywas 91% and 78% , respectively. Its similarity to other PR-1 genes was 57% to 60%. Southern-blot analysis indicated that there were at least two copies of BcPR-1 gene in B. rapa ssp. chinensis genome. Semi-quantitative RT-PCR analysis revealed that expression of BcPR-1 gene was induced by SA treatment in Suzhouqing, and the corresponding mRNA was accumulated most abundantly 12 h after treatment upon 2 mmol/L SA.

Key words: Brassica rapa ssp. chinensis, Pathogenesis-related protein 1, SA, RT-PCR

王彦华;;侯喜林;申书兴. 白菜病程相关蛋白1基因cDNA全长的克隆与分析[J]. 园艺学报, 2006, 33(5): 985-988.

Wang Yanhua;;Hou Xilin;Shen Shuxing. Cloning and Characterization of a Full-length cDNA of Pathogenesis-relatedProtein 1 in Brassica rapa ssp. chinensis[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 985-988.

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