番茄果实叶绿素代谢转录调控网络研究进展

doi:10.16420/j.issn.0513-353x.2022-0110

摘要/Abstract

摘要：

番茄（Solanum lycopersicum）果实中的叶绿素含量影响包括糖代谢在内的众多代谢过程，进而影响番茄成熟后的品质。叶绿素含量在很大程度上受转录水平调控。在介绍叶绿素合成与降解过程基础上，重点综述了BEL1-LIKE HOMEODOMAIN、MYB和ARF等转录因子家族对番茄果实叶绿素代谢相关靶基因（比如POR、SGR和CAB）的调控机制，同时也对不同转录因子的上下游互作关系及果实叶绿素代谢调控网络的研究进行了展望。

关键词: 番茄, 果实, 叶绿素代谢, 转录因子, 调控机制, 靶基因, 调控网络

Abstract:

The chlorophyll content of tomato（Solanum lycopersicum）fruit modulates many metabolic processes including sugar metabolism，and therefore affects the quality of ripe fruit. Chlorophyll content is largely regulated at the transcriptional level. In the current review，the synthesis and degradation process of chlorophyll was first introduced. The regulation mechanism of transcription factor families including BEL1-LIKE HOMEODOMAIN，MYB and ARF on genes related to chlorophyll metabolism（such as POR，SGR and CAB）in tomato fruits was reviewed. The interactions between different transcription factors and the regulatory network of chlorophyll metabolism in toamto fruits were also prospected.

Key words: tomato, fruit, chlorophyll metabolism, transcriptional factor, target gene, regulatory network

张巧丽, 陈笛, 宋艳萍, 朱鸿亮, 罗云波, 曲桂芹. 番茄果实叶绿素代谢转录调控网络研究进展[J]. 园艺学报, 2023, 50(9): 2031-2047.

ZHANG Qiaoli, CHEN Di, SONG Yanping, ZHU Hongliang, LUO Yunbo, QU Guiqin. Review on Transcriptional Regulation of Chlorophyll Metabolism Network in Tomato Fruits[J]. Acta Horticulturae Sinica, 2023, 50(9): 2031-2047.

图/表 4

图1 高等植物中叶绿素合成与降解途径

Fig. 1 Chlorophyll synthesis and degradation pathway in higher plant Barry et al.，2008；Tanaka et al.，2011；Lin et al.，2016；Tian et al.，2021.

图2 不同发育阶段番茄果实中叶绿素合成与降解酶基因的转录丰度热图测序数据来自Sato et al.，2012。1 cm、2 cm、3 cm表示果实直径；MG：绿熟期；B：破色期；B+10：破色后10 d。PPHL：脱镁叶绿素酶类似物。

Fig. 2 Heatmap of the Ttranscript abundance of genes involved in chlorophyll synthesis and degradation in tomato fruits at different developmental stages RNA-squencing data from Sato et al.，2012. 1 cm，2 cm，3 cm represent diameter of tomato fruits. MG：Mature green stage；B：Breaker stage；B+10：10 days after the breaker stage；PPHL：PPH-like.

表1 调控番茄果实叶绿素代谢转录因子转基因果实表型及其靶基因

Table 1 Transgenic fruit phenotypes and target genes of transcription factors regulating chlorophyll metabolism in tomato fruits

转录因子（基因号） Transcription factor（Gene ID）	野生型 Wild type	已报道的靶基因 The reported target genes	结合序列 Binding sequence	参考文献 Reference
BEL2（Solyc06g074120）		GLK2	串联TTGAC 基序 Tandem TTGAC motif	Niu et al.,2022
BL4（Solyc08g065420）		CHLD，PPO，TKN2， CAB	（G/A）GCCCA（A/T/C）	Yan et al.,2020
BEL11（Solyc11g068950）		POR1，TKN2，CAB	串联TGAC基序 Tandem TTGAC motif	Meng et al.,2018
ZHD17（Solyc04g080490）		TKN2，POR1，SGR1	ATTA/TAAT	Shi et al.,2021
MYB72（Solyc07g055000）		CHLH，POR1，TKN2	ACCAAC/ACCAAA	Wu et al.,2020
TAGL1（Solyc07g055920）	—	MPEC/MgCY，CAB， PsbQ	—	Liu et al.,2020
ARF6A（Solyc12g006340）		CAB，RbcS，GLK1	TGTCTC	Yuan et al.,2019
ARF10（Solyc11g069500）		POR2，CAB，GLK1	TGTCTC	Yuan et al.,2018
ARF4（Solyc11g069190）		—	TGTCTC	Sagar et al.,2013
GLK2（Solyc10g008160）		LHCBII	CCAATC	Waters et al.,2009；Powell et al.,2012；Nguyen et al.,2014；Niu et al.,2022
APRR2-like（Solyc08g077230）		—	—	Pan et al.,2013

图3 番茄果实叶绿素代谢与叶绿体发育转录调控网络

Fig. 3 Transcriptional regulation network of chlorophyll metabolism and chloroplast development in tomato fruits

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