铁十字秋海棠GASA家族全基因组鉴定及叶斑形成的初步探索

doi:10.16420/j.issn.0513-353x.2022-0472

园艺学报 ›› 2023, Vol. 50 ›› Issue (7): 1482-1494.doi: 10.16420/j.issn.0513-353x.2022-0472

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

铁十字秋海棠GASA家族全基因组鉴定及叶斑形成的初步探索

薛珍珍¹, 关鸿发³, 李娜², 李凌飞²^,^*(), 钟春梅¹^,^*()

¹ 华南农业大学生物质工程研究院，农业农村部能源植物资源与利用重点实验室，广东省农林生物质工程技术研究中心，广州 510642
² 深圳市中国科学院仙湖植物园，深圳市南亚热带植物多样性重点实验室，广东深圳 518004
³ 华南农业大学生命科学学院，广州 510642

收稿日期:2023-02-26 修回日期:2023-05-15 出版日期:2023-07-25 发布日期:2023-07-26
通讯作者:
^*（E-mail：zhongchunmei@scau.edu.cn，
lingfei_li@szbg.ac.cn）
基金资助:
广东省基础与应用基础研究基金项目(2021A1515011315); 深圳市城管科研项目(202205); 仙湖植物园科研基金项目(FLSF-2021-01)

Genome-wide Identification of GASA Family and Preliminary Exploration of Leaf Variegation Formation in Begonia masoniana

XUE Zhenzhen¹, GUAN Hongfa³, LI Na², LI Lingfei²^,^*(), ZHONG Chunmei¹^,^*()

¹ Key Laboratory of Energy Plants Resource and Utilization，Ministry of Agriculture and Rural Affairs，Guangdong Engineering Technology Research Center of Agricultural and Forestry Biomass，Institute of Biomass Engineering，South China Agricultural University，Guangzhou 510642，China
² Key Laboratory of Southern Subtropical Plant Diversity，Fairy Lake Botanical Garden，Shenzhen & Chinese Academy of Sciences，Shenzhen，Guangdong 518004，China
³ College of Life and Science，South China Agricultural University，Guangzhou 510642，China

Received:2023-02-26 Revised:2023-05-15 Published:2023-07-25 Online:2023-07-26

摘要/Abstract

摘要：

为了解铁十字秋海棠（Begonia masoniana）中Gibberellic Acid-stimulated Arabidopsis（GASA）基因家族的功能，采用生物信息的方法鉴定铁十字秋海棠GASA家族成员，并分析其在不同组织器官以及赤霉素（GA）、脱落酸（ABA）、水杨酸（SA）、茉莉酸甲酯（MeJA）、生长素（auxin）等处理后的基因表达模式。结果显示，铁十字秋海棠含10个GASA家族成员，可分为4个亚家族，其蛋白结构特征是含2 ~ 3个保守motif，1个GASA保守结构域；其基因结构特征是含0 ~ 10个内含子，不同成员之间内含子数差异明显。该家族基因启动子含有大量光响应元件和激素响应元件。基因家族成员在各组织器官中广泛表达，但差异较大；对外源GA、ABA、SA、MeJA以及auxin等响应差异明显，可能参与了不同组织器官生长发育以及各种生物或非生物胁迫响应过程，其中BmaGASA7可能在叶斑形成过程中发挥重要作用。

关键词: 铁十字秋海棠, GASA, 激素响应, 表达分析, 叶斑

Abstract:

In order to understand the potential functions of Gibberellic Acid-stimulated Arabidopsis （GASA）in Begonia masoniana（BmaGASAs），the bioinformatics analysis was carried out to identify the members of BmaGASA gene family，and then their expression patterns were analyzed. The results showed that ten members of the GASA family were distributed in four subgroups. The structural characteristics of BmaGASAs include 2-3 motifs，one GASA conserved domain，0-10 introns that varies among different members. The cis-acting elements distribution analysis showed that the BmaGASAs promoters contained abundant light and hormone response elements. BmaGASAs were widely expressed in various tissues and organs. In addition，BmaGASAs had significant differences in response to GA，ABA，SA，MeJA and auxin. This study indicates that members of the BmaGASA gene family are highly conserved and have diverse expression patterns. They may be involved in the growth and development of various tissues and organs，as well as the response processes of various biological or abiotic stress. Among them，BmaGASA7 may play an important role in leaf variegation formation.

Key words: Begonia masoniana, GASA, hormonal response, expression analysis, leaf variegation

薛珍珍, 关鸿发, 李娜, 李凌飞, 钟春梅. 铁十字秋海棠GASA家族全基因组鉴定及叶斑形成的初步探索[J]. 园艺学报, 2023, 50(7): 1482-1494.

XUE Zhenzhen, GUAN Hongfa, LI Na, LI Lingfei, ZHONG Chunmei. Genome-wide Identification of GASA Family and Preliminary Exploration of Leaf Variegation Formation in Begonia masoniana[J]. Acta Horticulturae Sinica, 2023, 50(7): 1482-1494.

图/表 7

表2 BmaGASA家族基本理化性质分析

Table 2 Basic physicochemical property of BmGASA family

基因 Gene	CDS/bp	氨基酸数 Amino acid number	等电点 pI	分子量/kD Molecular weight	染色体位置 Chr.	注释到拟南芥中 Annotated members of Arabidopsis thaliana	糖基化位点 N-glycosyl sites	亚细胞定位 Subcellular location
BmaGASA1	255	84	8.42	9.28	Chr01	GASA10	0	细胞壁 Cytoderm
BmaGASA2	432	143	9.40	15.13	Chr01	GASA14	0	细胞核 Nucleus
BmaGASA3	330	109	9.46	12.20	Chr01	GASA4	1	线粒体 Mitochondria
BmaGASA4	201	66	8.95	7.55	Chr02	GASA4	0	细胞核 Nucleus
BmaGASA5	309	102	9.18	10.74	Chr03	GASA1	0	细胞壁 Cytoderm
BmaGASA6	240	79	9.66	6.73	Chr04	GASA1	0	细胞核 Nucleus
BmaGASA7	183	60	9.20	6.50	Chr06	GASA1	1	线粒体 Mitochondria
BmaGASA8	270	89	9.26	10.12	Chr08	GASA7	0	细胞壁 Cytoderm
BmaGASA9	195	64	9.05	7.27	Chr13	GASA5	0	线粒体 Mitochondria
BmaGASA10	183	60	9.14	6.65	Chr14	GASA6	0	线粒体 Mitochondria

图 1 BmaGASA在染色体上的位置图示

Fig. 1 Chromosome location of BmaGASA family genes

图2 铁十字秋海棠（Bma）和拟南芥（At）、大豆（Gm）、苹果（Md）GASA家族成员系统发育树

Fig. 2 Phylogenetic tree of GASA family members of Arabidopsis thaliana（At），Glycine max（Gm），Malus × domestica（Md）and Begonia masoniana（Bma）

图3 铁十字秋海棠GASA家族成员基因结构（A）、motif分布（B）和保守结构域（C）分布图

Fig. 3 Genetic structure（A），conserved motif distribution（B），and conserved domain distribution（C）of BmaGASA family

图4 铁十字秋海棠GASA家族启动子序列顺式作用元件数量及种类

Fig. 4 Number and type of cis-acting elements in GASA family promoter sequence of Begonia masoniana

图5 铁十字秋海棠GASA家族基因在不同组织器官中的相对表达量内参基因为ACT7，相对基因表达为平均值 ± 标准差。叶片红色部分即为叶斑部分。BamGASA在不同组织中最小表达量视为“1”。

Fig. 5 Relative expression levels of GASA gene family in different tissues and organs of Begonia masoniana The reference gene was ACT7，and the relative gene expression was shown as the mean ± SD. The red part of the leaf is the leaf variegation part. The minimum expression of BamGASAs in different tissues was regarded as“1”.

图 6 铁十字秋海棠GASA家族基因在GA、ABA、SA、MeJA、NAA处理（6 h）下的相对表达量将对照的相对表达量视为“1”。*、**、***分别表示在0.05、0.01、0.001水平上差异显著（t检验）。

Fig. 6 Relative expression levels of GASA gene family in Begonia masoniana under GA, ABA, SA, MeJA and NAA treatments（6 h） The relative expression of control is regarded as“1”. *，** and *** represent significant differences at the levels of 0.05，0.01 and 0.001 respectively（t-test）.

参考文献 46

[1]	Abdullah, Faraji S, Mehmood F, Malik H M T, Ahmed I, Heidari P, Poczai P. 2021. The GASA gene family in cacao(Theobroma cacao, Malvaceae):Genome wide identification and expression analysis. Agronomy-Basel, 11 (7):1425.
[2]	Ahmad B, Yao J, Zhang S, Li X, Zhang X, Yadav V, Wang X. 2020. Genome-wide characterization and expression profiling of GASA genes during different stages of seed development in grapevine(Vitis vinifera L.) predict their involvement in seed development. International Journal of Molecular Sciences, 21 (3):1088. doi: 10.3390/ijms21031088 URL
[3]	Ahmad M Z, Sana A, Jamil A, Nasir J A, Ahmed S, Hameed M U, Abdullah. 2019. A genome-wide approach to the comprehensive analysis of GASA gene family in Glycine max. Plant Molecular Biology, 100 (6):607-620.
[4]	Aubert D, Chevillard M, Dorne A M, Arlaud G, Herzog M. 1998. Expression patterns of GASA genes in Arabidopsis thaliana: the GASA4 gene is up-regulated by gibberellins in meristematic regions. Plant Molecular Biology, 36 (6):871-883. doi: 10.1023/a:1005938624418 pmid: 9520278
[5]	Ben-Nissan G, Lee J Y, Borohov A, Weiss D. 2004. GIP,a Petunia hybrida GA-induced cysteine-rich protein:a possible role in shoot elongation and transition to flowering. Plant Journal, 37 (2):229-238. doi: 10.1046/j.1365-313x.2003.01950.x pmid: 14690507
[6]	Ben-Nissan G, Weiss D. 1996. The petunia homologue of tomato gast1:Transcript accumulation coincides with gibberellin-induced corolla cell elongation. Plant Molecular Biology, 32 (6):1067-1074. doi: 10.1007/BF00041390 pmid: 9002605
[7]	Chen C, Chen H, Zhang Y, Thomas H R, Frank M H, He Y, Xia R. 2020. TBtools:an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13 (8):1194-1202. doi: 10.1016/j.molp.2020.06.009 URL
[8]	Chen Junjie, Mei Song, Hu Yanru. 2020. Abscisic acid induces anthocyanin synthesis in Arabidopsis thaliana seedlings. Guihaia, 40 (8):1169-1180. (in Chinese)
	陈俊洁, 梅松, 胡彦如. 2020. 脱落酸激素诱导拟南芥幼苗中花青素的合成. 广西植物, 40 (8):1169-1180.
[9]	Cui Weihua, Guan Kaiyun. 2013. Diversity of leaf variegation in Chinese Begonias. Plant Diversity and Resources, 35 (2):119-127. (in Chinese)
	崔卫华, 管开云. 2013. 中国秋海棠属植物叶片斑纹多样性研究. 植物分类与资源学报, 35 (2):119-127.
[10]	Chen Yazhou. 2008. Glucosinolate accumulation response to exogenous methyl jasmonate in Arabidopsis thaliana[M. D. Dissertation]. Harbin:Northeast Forestry University. (in Chinese)
	陈亚州. 2008. 拟南芥芥子油苷积累对外源茉莉酸甲酯的响应[硕士论文]. 哈尔滨: 东北林业大学.
[11]	Cremer T, Cremer M, Dietzel S, Mueller S, Solovei I, Fakan S. 2006. Chromosome territories-a functional nuclear landscape. Current Opinion in Cell Biology, 18 (3):307-316. doi: 10.1016/j.ceb.2006.04.007 URL
[12]	de la Fuente J I, Amaya I, Castillejo C, Sanchez-Sevilla J F, Quesada M A, Botella M A, Valpuest V. 2006. The strawberry gene FaGAST affects plant growth through inhibition of cell elongation. Journal of Experimental Botany, 57 (10):2401-2411. pmid: 16804055
[13]	Fan S, Zhang D, Zhang L, Gao C, Xin M, Tahir M M, Li Y, Ma J, Han M. 2017. Comprehensive analysis of GASA family members in the Malus domestica genome:Identification,characterization,and their expressions in response to apple flower induction. BMC Genomics, 18 (1):827. doi: 10.1186/s12864-017-4213-5 URL
[14]	Han S, Jiao Z, Niu M X, Yu X, Huang M, Liu C, Wang H L, Zhou Y, Mao W, Wang X, Yin W, Xia X. 2021. Genome-wide comprehensive analysis of the GASA gene family in Populus. International Journal of Molecular Sciences, 22 (22):12336. doi: 10.3390/ijms222212336 URL
[15]	He H, Yang X, Xun H, Lou X, Li S, Zhang Z, Jiang L, Dong Y, Wang S, Pang J, Liu B. 2017. Over-expression of GmSN1 enhances virus resistance in Arabidopsis and soybean. Plant Cell Reports, 36 (9):1441-1455. doi: 10.1007/s00299-017-2167-3 URL
[16]	Hong X, Scofield D G, Lynch M. 2006. Intron size,abundance,and distribution within untranslated regions of genes. Molecular Biology and Evolution, 23 (12):2392-2404. pmid: 16980575
[17]	Huang Yali, Zhang Jun, Fan Yingli, Liu Yichao, Yang Minsheng. 2019. Effects of shading treatmentson leaf color and related physiological indexes of Ulmus pumila‘Jinye’and Koelreuteria paniculata‘Xinye’. Scientia Silvae Sinicae, 55 (10):171-180. (in Chinese)
	黄亚丽, 张军, 樊英利, 刘易超, 杨敏生. 2019. 遮荫对中华金叶榆和鑫叶栾叶片呈色及相关生理指标的影响. 林业科学, 55 (10):171-180.
[18]	Kazem G, Ayda H, Salar F. 2020. Chlorophyll a fluorescence of safflower affected by salt stress and hormonal treatments. SN Applied Sciences, 2 (7):2523-3963.
[19]	Li L, Chen X, Fang D, Dong S, Guo X, Li N, Campos-Dominguez L, Wang W, Liu Y, Lang X, Peng Y, Tian D, Thomas D C, Mu W, Liu M, Wu C, Yang T, Zhang S, Yang L, Yang J, Liu Z, Zhang L, Zhang X, Chen F, Jiao Y, Guo Y, Hughes M, Wang W, Liu X, Zhong C, Li A, Sahu S K, Yang H, Wu E, Sharbrough J, Lisby M, Liu X, Xu X, Soltis D E, van de Peer Y, Kidner C, Zhang S, Liu H. 2022. Genomes shed light on the evolution of Begonia,a mega-diverse genus. New Phytologist, 234 (1):295-310. doi: 10.1111/nph.v234.1 URL
[20]	Li Yueya, He Dong, Chen Jiancun, Li Bo, Bai Meng, Luo Le, Zhang Qixiang. 2022. Identification and analysis of the type of leaf variegation of Primulina pungentisepala. Acta Horticulturae Sinica, 49 (11):2388-2394. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-1038
	李悦雅, 何栋, 陈简村, 李博, 白梦, 罗乐, 张启翔. 2022. 尖萼报春苣苔叶斑的观察与分析. 园艺学报, 49 (11):2388-2394. doi: 10.16420/j.issn.0513-353x.2021-1038
[21]	Lü Liangjie, Chen Xiyong, Zhang Yelun, Liu Qian, Wang Limei, Ma Le, Li Hui. 2018. Bioinformatics identification of GASA gene family expression profiles in wheat. Crops,(6):58-67. (in Chinese)
	吕亮杰, 陈希勇, 张业伦, 刘茜, 王莉梅, 马乐, 李辉. 2018. 小麦GASA基因家族生物信息学分析. 作物杂志,(6):58-67.
[22]	Li Kunlun, Bo Xi, Lu Shan, Yuan Yue, Li Yong, Ji Wei, Cai Hua, Ji Zuojun, Zhu Yanming. 2012. Expression analysis of two alkali stress related genes GsGASA1 and GsGASA2. Journal of Northeast Agricultural University, 43 (1):143-148. (in Chinese)
	李昆仑, 柏锡, 卢姗, 袁悦, 李勇, 纪巍, 才华, 季佐军, 朱延明. 2012. 碱胁迫应答GsGASA1及GsGASA2基因表达特性研究. 东北农业大学学报, 43 (1):143-148.
[23]	Liechti R, Farmer E E. 2006. Jasmonate biochemical pathway. Science’s STKE,(322):cm3.
[24]	Liu Qiuhua, Luo Man, Peng Jianzong, Wang XiaoJing. 2015. Isolation and analysis of rice OsGASR4 gene and its promoter. Journal of South China Normal University(Natural Science Edition), 47 (1):81-86. (in Chinese)
	刘秋华, 罗曼, 彭建宗, 王小菁. 2015. 水稻OsGASR4基因及其启动子的克隆与表达分析. 华南师范大学学报(自然科学版), 47 (1):81-86.
[25]	Moyano-Canete E, Bellido M L, Garcia-Caparros N, Medina-Puche L, Amil-Ruiz F, Gonzalez-Reyes J A, Caballero J L, Munoz-Blanco J, Blanco-Portales R. 2013. FaGAST2,a strawberry ripening-related gene,acts together with FaGAST1 to determine cell size of the fruit receptacle. Plant and Cell Physiology, 54 (2):218-236. doi: 10.1093/pcp/pcs167 pmid: 23231876
[26]	Nahirnak V, Almasia N I, Hopp H E, Vazquez-Rovere C. 2012. Snakin/GASA proteins:involvement in hormone crosstalk and redox homeostasis. Plant Signaling & Behavior, 7 (8):1004-1008.
[27]	Parra G, Bradnam K, Rose A B, Korf I. 2011. Comparative and functional analysis of intron-mediated enhancement signals reveals conserved features among plants. Nucleic Acids Research, 39 (13):5328-5337. doi: 10.1093/nar/gkr043 pmid: 21427088
[28]	Qu J, Kang S G, Hah C, Jang J C. 2016. Molecular and cellular characterization of GA-stimulated transcripts GASA4 and GASA6 in Arabidopsis thaliana. Plant Science, 246:1-10. doi: 10.1016/j.plantsci.2016.01.009 URL
[29]	Reymond P, Farmer E E. 1998. Jasmonate and salicylate as global signals for defense gene expression. Current Opinion in Plant Biology, 1 (5):404-411. doi: 10.1016/s1369-5266(98)80264-1 pmid: 10066616
[30]	Roxrud I, Lid S E, Fletcher J C, Schmidt E D L, Opsahl-Sorteberg H. 2007. GASA4,one of the 14-member Arabidopsis GASA family of small polypeptides,regulates flowering and seed development. Plant and Cell Physiology, 48 (3):471-483. doi: 10.1093/pcp/pcm016 URL
[31]	Rubinovich L, Ruthstein S, Weiss D. 2014. The Arabidopsis cysteine-rich GASA 5 is a redox-active metalloprotein that suppresses gibberellin responses. Molecular Plant, 7 (1):244-247. doi: 10.1093/mp/sst141 pmid: 24157610
[32]	Silverstein K A T Jr. Moskal W A, Wu H C, Underwood B A, Graham M A, Town C D, VandenBosch K A. 2007. Small cysteine-rich peptides resembling antimicrobial peptides have been under-predicted in plants. Plant Journal, 51 (2):262-280. doi: 10.1111/j.1365-313X.2007.03136.x pmid: 17565583
[33]	Sun S, Wang H, Yu H, Zhong C, Zhang X, Peng J, Wang X. 2013. GASA14regulates leaf expansion and abiotic stress resistance by modulating reactive oxygen species accumulation. Journal of Experimental Botany, 64 (6):1637-1647. doi: 10.1093/jxb/ert021 URL
[34]	Wang Hongfei, Shang Qingmao. 2019. Genome-wide identification and expression analysis of the SAUR gene family in Cucumis sativus. Acta Horticulturae Sinica, 46 (6):1093-1111. (in Chinese)
	王红飞, 尚庆茂. 2019. 黄瓜SAUR基因家族的鉴定与表达分析. 园艺学报, 46 (6):1093-1111.
[35]	Wang H, Wei T, Wang X, Zhang L, Yang M, Chen L, Song W, Wang C, Chen C. 2018. Transcriptome analyses from mutant Salvia miltiorrhiza reveals important roles for SmGASA4 during plant development. International Journal of Molecular Sciences, 19 (7):2088. doi: 10.3390/ijms19072088 URL
[36]	Yang Ting, Xue Zhenhen, Li Na, Lang Xiaoan, Li Lingfei, Zhong Chunmei. 2021. Reference genes selection and validation in Begonia masoniana leaves of different developmental stages. Acta Horticulturae Sinica, 48 (11):2251-2261. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-0397
	杨婷, 薛珍珍, 李娜, 郎校安, 李凌飞, 钟春梅. 2021. 铁十字秋海棠斑叶发育过程内参基因筛选及验证. 园艺学报, 48 (11):2251-2261. doi: 10.16420/j.issn.0513-353x.2021-0397
[37]	Yang Q, Niu Q, Tang Y, Ma Y, Yan X, Li J, Tian J, Bai S, Teng Y. 2019. PpyGAST1 is potentially involved in bud dormancy release by integrating the GA biosynthesis and ABA signaling in‘Suli’pear(Pyrus pyrifolia white pear group). Environmental and Experimental Botany, 162:302-312. doi: 10.1016/j.envexpbot.2019.03.008 URL
[38]	Yu Zhiman. 1987. Begonia masoniea. Life Word,( 3):19-20. (in Chinese)
	余志满. 1987. 铁十字秋海棠. 植物杂志,(3):19-20.
[39]	Zhang S, Wang X. 2017. One new kind of phytohormonal signaling integrator:up-and-coming GASA family genes. Plant Signaling & Behavior, 12 (2):1559-2316.
[40]	Zhang Shuang, Li Ruiyi, Ji Chunyan, Zhao Yahui. 2016. The cultivation and maintenance of the Begonia masoniana and its application in garden. Forest By-Product and Speciality in China,(6):45-46. (in Chinese)
	张爽, 李睿怡, 纪春艳, 赵雅卉. 2016. 铁十字秋海棠的栽培养护及园林应用. 中国林副特产,(6):45-46.
[41]	Zhong Chunmei, Wang Xiaojing. 2016. Progress in cysteine-rich Gibberellic Acid-stimulated Arabidopsis protein. Chinese Bulletin of Botany, 51 (1):1-8. (in Chinese)
	钟春梅, 王小菁. 2016. 富含半胱氨酸的GASA小分子蛋白研究进展. 植物学报, 51 (1):1-8. doi: 10.11983/CBB15118
[42]	Zhang S, Wang X. 2008. Expression pattern of GASA,downstream genes of DELLA,in Arabidopsis. Chinese Science Bulletin, 53 (24):3839-3846.
[43]	Zhang S, Yang C, Peng J, Sun S, Wang X. 2009. GASA5,a regulator of flowering time and stem growth in Arabidopsis thaliana. Plant Molecular Biology, 69 (6):745-759. doi: 10.1007/s11103-009-9452-7 URL
[44]	Zhong C, Xu H, Ye S, Wang S, Li L, Zhang S, Wang X. 2015. Gibberellic acid-stimulated Arabidopsis 6 serves as an integrator of gibberellin,abscisic acid,and glucose signaling during seed germination in Arabidopsis. Plant Physiology, 169 (3):2288-2303.
[45]	Zimmermann R, Sakai H, Hochholdinger F. 2010. The Gibberellic Acid Stimulated-Like gene family in maize and its role in lateral root development. Plant Physiology, 152 (1):356-365. doi: 10.1104/pp.109.149054 pmid: 19926801
[46]	Zhang Shengchun. 2008. Analysis of GASA gene family expression and function of GASA5 in Arabidopsis thaliana[Ph. D. Dissertation]. South China Normal University. (in Chinese)
	张盛春. 2008. 拟南芥GASA基因家族表达分析及GASA5功能研究[博士论文]. 广州: 华南师范大学.

铁十字秋海棠GASA家族全基因组鉴定及叶斑形成的初步探索

Genome-wide Identification of GASA Family and Preliminary Exploration of Leaf Variegation Formation in Begonia masoniana

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铁十字秋海棠GASA家族全基因组鉴定及叶斑形成的初步探索

Genome-wide Identification of GASA Family and Preliminary Exploration of Leaf Variegation Formation in Begonia masoniana

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