白菜耐光氧化能力鉴定及其与活性氧代谢关系的研究

园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 979-984.

白菜耐光氧化能力鉴定及其与活性氧代谢关系的研究

黄俊;吴震^*;郭世荣

(南京农业大学园艺学院, 江苏南京 210095)

收稿日期:2005-11-24 修回日期:2006-05-15 出版日期:2006-10-25 发布日期:2006-10-25

Identification of Photooxidation Tolerance and Its relationship with Metabolism of Reactive Oxygen Species in Pak-choi

Huang Jun;Wu Zhen^*;Guo Shirong

(College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China)

Received:2005-11-24 Revised:2006-05-15 Online:2006-10-25 Published:2006-10-25

摘要/Abstract

摘要： 采用人工光氧化技术对30个常见白菜栽培品种进行光氧化处理, 以总叶绿素存留率作为评价指标, 利用系统聚类分析将供试品种划分为5个级别(1～5级) 。其中耐光氧化能力强的品种有青优4号、暑绿、抗热605、绿扬青、矮抗6号、华王; 不耐光氧化的品种有四倍体矮脚黄、绿星。在此基础上, 以耐光氧化品种抗热605与不耐光氧化品种绿星为材料, 研究了光氧化过程中活性氧代谢的特征。结果表明:在光氧化条件下, 白菜叶片内的抗氧化酶SOD、POD、CAT、APX活性受光氧化诱导升高, 但光氧化敏感性品种绿星的酶活性很快下降, 耐光氧化品种抗热605的酶活持续保持在较高的水平。因此, 绿星的O₂·生成速率、H₂O₂含量及MDA的积累量均高于抗热605, 最终导致品种绿星在光氧化下总叶绿素存留率低。表明抗热605植株内较高的抗氧化酶活性对提高耐光氧化能力起了重要作用。

关键词: 白菜, 光氧化, 活性氧代谢, 抗氧化酶

Abstract: Thirty cultivars of pak-choi (Brassica campestris ssp. chinensis Makino) were divided into five grades according to their photooxidation tolerance by the residual content of total chlorophylls under artificial photooxidation condition, where the tolerant ones included Qingyou 4, Shulü, Kangre 605, Lüyang Qing, Aikang 6 and Huawang, and the suscep tible oneswere Lüxing and Aijiao Huang. In order to study the characteristics of reactive oxygen metabolism between pak-chio varietieswith different photooxidation tolerance, Kangre 605
and Lüxingwere used to compare the differences in the paper. The results showed that the activities of antioxidant enzymes including superoxide dismutase (SOD) , peroxidase (POD) , catalase (CAT) and ascorbate peroxidase (APX) in the two cultivarswere induced at the beginning of photooxidation stress, and then decreased in the sensitive Lüxing, but that of the tolerant Kangre 605 continued to keep at a higher levels. Additionally, the O₂·production rate and the content of H₂O₂ and malondialdehyde (MDA) in Lüxing were higher than that in Kangre 605, whichmight be the cause leading to a lower level of chlorophylls. These results suggested that higher antioxidant enzyme activities p layed an important role in Kangre 605 against photooxidation stress.

Key words: Pak-choi, Photooxidation, Reactive oxygen species, Antioxidant enzymes

黄俊;吴震;郭世荣. 白菜耐光氧化能力鉴定及其与活性氧代谢关系的研究[J]. 园艺学报, 2006, 33(5): 979-984.

Huang Jun;Wu Zhen;Guo Shirong. Identification of Photooxidation Tolerance and Its relationship with Metabolism of Reactive Oxygen Species in Pak-choi[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 979-984.

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