园艺学报 ›› 2023, Vol. 50 ›› Issue (5): 1063-1072.doi: 10.16420/j.issn.0513-353x.2022-0155
高美娜, 孙明飞, 朱杰, 井俊丽, 李佳, 周莎莎, 梁博文, 徐继忠, 李中勇()
收稿日期:
2022-12-31
修回日期:
2023-03-23
出版日期:
2023-05-25
发布日期:
2023-05-31
通讯作者:
*(E-mail:yylzy2014@163.com)基金资助:
GAO Meina, SUN Mingfei, ZHU Jie, JING Junli, LI Jia, ZHOU Shasha, LIANG Bowen, XU Jizhong, LI Zhongyong()
Received:
2022-12-31
Revised:
2023-03-23
Published:
2023-05-25
Online:
2023-05-31
摘要:
以苹果矮化砧木‘冀砧2号’为试材,研究绞缢、环割压条生根效果和IAA含量及相关指标变化,结果表明:绞缢处理的压条生根率为78.92%,显著高于环割和对照;压条生根过程中,绞缢处理的IAA含量显著高于环割和对照,35 d时出现最大值,为39.93 ng · g-1;不定根诱导期和形成期,绞缢处理的POD、PPO活性显著高于环割和对照,IAAO活性均低于环割和对照;不定根诱导期,绞缢处理的MdPAT1、MdPIN1相对表达量逐渐上升,35 d时相对表达量最高,显著高于对照;25 d时MdYUCCA4相对表达量显著高于环割和对照,MdARF17的相对表达量则显著低于环割和对照。综上,绞缢可显著提高‘冀砧2号’砧木压条生根率和生根指数,生长素IAA对‘冀砧2号’不定根根原基的诱导和促进不定根伸长具有重要作用。
中图分类号:
高美娜, 孙明飞, 朱杰, 井俊丽, 李佳, 周莎莎, 梁博文, 徐继忠, 李中勇. 苹果砧木‘冀砧2号’绞缢、环割压条生根效果及过程中IAA含量的变化[J]. 园艺学报, 2023, 50(5): 1063-1072.
GAO Meina, SUN Mingfei, ZHU Jie, JING Junli, LI Jia, ZHOU Shasha, LIANG Bowen, XU Jizhong, LI Zhongyong. The Rooting Effect and Changes of IAA Content in Constriction,Girdling Treatment During Shoot Layering of Apple Rootstock‘Jizhen 2’[J]. Acta Horticulturae Sinica, 2023, 50(5): 1063-1072.
基因名称 | 基因ID | 引物序列 | |
---|---|---|---|
Gene name | Gene ID | Primer sequence | |
β-Actin | LOC103453508 | F:5′-GGATTTGCTGGTGATGATGCT-3′ | R:5′-AGTTGCTCACTATGCCGTGCT-3′ |
PIN1 | LOC103455629 | F:5′-TGCTACTGGAAACAAGCCGA-3′ | R:5′-GCATTAGCATTAGCGATGACGG-3′ |
PAT1 | LOC103442977 | F:5′-GCGTTGAGAGGGTGGAAAGA-3′ | R:5′-GATAGAGTGCCCCATCTCGC-3′ |
ARF17 | LOC103401869 | F:5′-GCCCAATGGCGACAAACTC-3′ | R:5′-GGAACAGAGAATCCACCCCC-3′ |
YUCCA4 | LOC103456153 | F:5′-CCTCCCACTTCTCAATCCACC-3′ | R:5′-GTGGCAACAATCAGCCACTT-3′ |
表1 实时荧光定量PCR基因引物序列
Table 1 Primer sequences for the quantification by real-time PCR
基因名称 | 基因ID | 引物序列 | |
---|---|---|---|
Gene name | Gene ID | Primer sequence | |
β-Actin | LOC103453508 | F:5′-GGATTTGCTGGTGATGATGCT-3′ | R:5′-AGTTGCTCACTATGCCGTGCT-3′ |
PIN1 | LOC103455629 | F:5′-TGCTACTGGAAACAAGCCGA-3′ | R:5′-GCATTAGCATTAGCGATGACGG-3′ |
PAT1 | LOC103442977 | F:5′-GCGTTGAGAGGGTGGAAAGA-3′ | R:5′-GATAGAGTGCCCCATCTCGC-3′ |
ARF17 | LOC103401869 | F:5′-GCCCAATGGCGACAAACTC-3′ | R:5′-GGAACAGAGAATCCACCCCC-3′ |
YUCCA4 | LOC103456153 | F:5′-CCTCCCACTTCTCAATCCACC-3′ | R:5′-GTGGCAACAATCAGCCACTT-3′ |
图1 苹果砧木‘冀砧2号’不定根形成过程解剖学结构 a:新梢基部的横切面;b:起源形成层处的根原基原始细胞;c:形成层处的根原基原始细胞聚集形成细胞团;d:根原基形成;e:不定根形成;f:不定根伸出表皮,不断伸长;g:愈伤组织中形成的根原基原始细胞;h:起源于髓射线的根原基。Ep:表皮;Ph:韧皮部;Xy:木质部;Xr:髓射线;Pi:髓;Vc:维管形成层;RPic:根原基原始细胞;Rp:根原基;Ar:不定根。
Fig. 1 Anatomic structure of adventitious root formation a:Transverse section of new shoot base;b:Primordial root cells originating from cambium;c:The primordial root cells originating from cambium aggregate to form a cell mass;d:Root primordium formation;e:Adventitious root formation;f:Adventitious root out of the epidermis,continuous elongation;g:Root primordium primitive cells formed in callus;h:Root primordia originating from medullary rays. Ep:Epidermis;Ph:Phloem;Xy:Xylem;Xr:Pith ray;Pi:Pulp;Vc:Vascular cambium;RPic:Root primordia primitive cell;Rp:Root primordia;Ar:Adventitious roots.
不同处理 | 生根率/% | 平均根长/cm | 单株平均根数 | 平均根粗/mm | 最长根长/mm | 生根指数 |
---|---|---|---|---|---|---|
Different treatments | Rooting rate | Length of roots | Roots number per plant | Width of root | The longest root | Rooting index |
对照 Control | 16.54 ± 3.38 c | 10.04 ± 2.73 b | 6.67 ± 1.15 b | 1.66 ± 0.27 c | 15.33 ± 3.51 b | 10.73 ± 1.46 c |
环割 Girdling | 24.85 ± 5.08 b | 18.72 ± 3.00 a | 6.75 ± 0.96 b | 2.75 ± 0.63 b | 32.00 ± 3.56 a | 31.28 ± 5.53 b |
绞缢 Constriction | 78.92 ± 9.46 a | 19.59 ± 3.80 a | 8.75 ± 1.26 a | 3.64 ± 0.29 a | 36.67 ± 5.13 a | 132.92 ± 13.52 a |
表2 绞缢和环割处理对苹果砧木‘冀砧2号’压条生根的影响
Table 2 Effects of constriction and girdling treatment on rooting of apple rootstock‘Jizhen 2’
不同处理 | 生根率/% | 平均根长/cm | 单株平均根数 | 平均根粗/mm | 最长根长/mm | 生根指数 |
---|---|---|---|---|---|---|
Different treatments | Rooting rate | Length of roots | Roots number per plant | Width of root | The longest root | Rooting index |
对照 Control | 16.54 ± 3.38 c | 10.04 ± 2.73 b | 6.67 ± 1.15 b | 1.66 ± 0.27 c | 15.33 ± 3.51 b | 10.73 ± 1.46 c |
环割 Girdling | 24.85 ± 5.08 b | 18.72 ± 3.00 a | 6.75 ± 0.96 b | 2.75 ± 0.63 b | 32.00 ± 3.56 a | 31.28 ± 5.53 b |
绞缢 Constriction | 78.92 ± 9.46 a | 19.59 ± 3.80 a | 8.75 ± 1.26 a | 3.64 ± 0.29 a | 36.67 ± 5.13 a | 132.92 ± 13.52 a |
图3 苹果砧木‘冀砧2号’压条生根过程中IAA含量和POD、IAAO、PPO活性变化 不同字母表示相同时期不同处理间差异显著(P < 0.05)。
Fig. 3 Changes in IAA content and POD,IAAO,PPO activities during rooting of apple rootstock‘Jizhen 2’ Different letters indicate significant differences between treatments in the same period(P < 0.05).
图4 苹果砧木‘冀砧2号’不定根诱导期生根相关基因表达量 不同字母表示相同时期不同处理间差异显著(P < 0.05)。
Fig. 4 Expression of rooting related genes during adventitious root induction of apple rootstock‘Jizhen 2’ Different letters indicate significant differences between treatments in the same period(P < 0.05).
[1] |
Chen Y, Fan X, Song W, Zhang Y, Xu G. 2012. Over-expression of OsPIN2 leads to increased tiller numbers,angle and shorter plant height through suppression of OsLAZY1. Plant Biotechnology Journal, 10 (2):139-149.
doi: 10.1111/pbi.2011.10.issue-2 URL |
[2] | Costa C, Almeida M D, Ruedell C M, Schwambach J, Maraschin F S, Fett-Neton A G. 2013. When stress and development go hand in hand:main hormonal controls of adventitious rooting in cuttings. Frontiers in Plant Science, 4:133. |
[3] |
Dawood T, Yang X, Visser E J, Te Beek T A, Kensche P R, Cristescu S M, Lee S, Floková K, Rieu I. 2016. A co-opted hormonal cascade activates dormant adventitious root primordia upon flooding in Solanum dulcamara. Plant Physiology, 170 (4):2351-2364.
doi: 10.1104/pp.15.00773 URL |
[4] |
Feng Yi, Xu Xuefeng, Zhang Xinzhong, Wu Ting, Wang Yi, Han Zhenhai. 2018. Progress of dwarfing mechanism of apple rootstock. Acta Horticulturae Sinica, 45 (9):1633-1641. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2018-0384 |
冯轶, 许雪峰, 张新忠, 吴婷, 王忆, 韩振海. 2018. 苹果矮化砧木致矮机理的研究进展. 园艺学报, 45 (9):1633-1641.
doi: 10.16420/j.issn.0513-353x.2018-0384 |
|
[5] | Gao Jun-feng. 2006. Experimental guidance of plant physiology. Beijing: Higher Education Press. (in Chinese) |
高俊凤. 2006. 植物生理学实验指导. 北京: 高等教育出版社. | |
[6] |
Guan L, Murphy A S, Peer W A, Gan L, Li Y, Cheng Z M. 2015. Physiological and molecular regulation of adventitious root formation. Critical Reviews in Plant Sciences, 34 (5):506-521.
doi: 10.1080/07352689.2015.1090831 URL |
[7] |
Gutierrez L, Bussell J D, Pacurar D I, Schwambach J, Pacurar M, Bellini C. 2009. Phenotypic plasticity of adventitious rooting in Arabidopsis is controlled by complex regulation of auxin response factor transcripts and microrna abundance. Plant Cell, 21 (10):3119-3132.
doi: 10.1105/tpc.108.064758 URL |
[8] | Huang Zhuo-lie, Li Ming, Tan Shao-man, Wu Guang-hong, Zhan Fu-jian, Long Teng. 2003. Effects of indolebutyric acid on polyphenol oxidase and its relationship with rooting in eucalyptus cuttings. Guangxi Plants,(1):77-82. (in Chinese) |
黄卓烈, 李明, 谭绍满, 巫光宏, 詹福建, 龙腾. 2003. 吲哚丁酸对桉树插条多酚氧化酶的影响及其与生根的关系. 广西植物,(1):77-82. | |
[9] |
Horton P, Park K J, Obayashi T, Fujita N, Harada H, Adams-Collier C J, Nakai K. 2007. WoLF PSORT:protein localization predictor. Nucleic Acids Research, 35 (Suppl_2):W585-W587.
doi: 10.1093/nar/gkm259 URL |
[10] | Jiang Zhong-guan. 2001. Experiments on propagation techniques of hazelnut in Europe. Northern Fruit Trees,(4):17-18. (in Chinese) |
姜忠官. 2001. 欧洲榛子繁殖技术试验. 北方果树,(4):17-18. | |
[11] |
Jung K, Hull A K, Kowalczyk M, Marchant A, Celenza J, Cohen J D, Sandberg G. 2002. Biosynthesis,conjugation,catabolism and homeostasis of indole-3-acetic acid in Arabidopsis thaliana. Plant Molecular Biology, 49 (3-4):249-272.
doi: 10.1023/A:1015298812300 URL |
[12] | Koo Y B, Hyun J O. 1996. Initial root development of Larix leptolepis gordon cuttings as related to organic substances and cutting date. Journal of Korean Forestry Society, 85 (2):300-308. |
[13] | Kose C, Erdal S, Kaya O, Atici O. 2011. Comparative evaluation of oxidative enzyme activities during adventitious rooting in the cuttings of grapevine rootstocks. Journal of the Science of Food & Agriculture, 91 (4):738-741. |
[14] |
Legué V, Rigal A, Bhalerao R P. 2014. Adventitious root formation in tree species:involvement of transcription factors. Physiologia Plantarum, 151 (2):192-198.
doi: 10.1111/ppl.12197 URL |
[15] | Li Da-wei. 2008. Study on cutting propagation technology and adventative root formation mechanism of hazelnut[M. D. Dissertation]. Beijing: Beijing Forestry University. (in Chinese) |
李大威. 2008. 榛子扦插繁殖技术及不定根发生机理研究[硕士论文]. 北京: 北京林业大学. | |
[16] | Megre D, Dokane K, Kondratovics U. 2011. Can changes in starch content and peroxidase activity be used as rooting phase markers for rhododendron leaf bud cuttings. Acta Biologica Cracoviensia, 53 (1):74-79. |
[17] | Mu Hong-xiang. 2003. Rapid propagation of triploid Populus tomentosa clones[M. D. Dissertation]. Tai’an: Shandong Agricultural University. (in Chinese) |
牟洪香. 2003. 三倍体毛白杨优良无性系微体快速繁殖技术研究[硕士论文]. 泰安: 山东农业大学. | |
[18] |
Naija S, Elloumi N, Jbir N, Ammar S, Kevers C. 2008. Anatomical and biochemical changes during adventitious rooting of apple rootstocks MM 106 cultured in vitro. Comptes Rendus Biologies, 331 (7):518.
doi: 10.1016/j.crvi.2008.04.002 URL |
[19] | Negishi N, Oishi M, Kawaoka A. 2011. Chemical screening for promotion of adventitious root formation in Eucalyptus globulus. BMC Proceedings, 5 (7):1. |
[20] |
Pacurar D I, Perrone B. 2014. Auxin is a central player in the hormone cross-talks that control adventitious rooting. Physiologia Plantarum, 151 (1):83-96.
doi: 10.1111/ppl.12171 URL |
[21] |
Rout G R. 2006. Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis(L.) Kuntze and associated biochemical changes. Plant Growth Regulation, 48 (2):111-117.
doi: 10.1007/s10725-005-5665-1 URL |
[22] | Satisha J, Raveendran P, Rokade N D. 2008. Changes in polyphenol oxidase activity during rooting of hardwood cuttings in three grape rootstocks under indian conditions. South African Journal of Enology & Viticulture, 29 (2):94-97. |
[23] | Sha Guang-li. 2020. Discussion on several problems of dwarfing stock and dwarfing cultivation of apple. China Fruit Trees,(3):6-10. (in Chinese) |
沙广利. 2020. 关于苹果矮化砧与矮化栽培几个问题的探讨. 中国果树,(3):6-10. | |
[24] | Song Peng-fei, Chen Hua-jiang, Jiang Yan-qin, Yu Hong, Qi Xing-jiang. 2014. Study on the morphology and anatomy of cutting roots of pink blue shoots of rabbit eye blue berry. Journal of Zhejiang Agricultural Sciences,(4):505-508. (in Chinese) |
宋鹏飞, 陈华江, 姜燕琴, 於虹, 戚行江. 2014. 兔眼蓝浆果粉蓝嫩枝扦插生根的形态和解剖研究. 浙江农业科学,(4):505-508. | |
[25] |
Sorin C. 2005. Auxin and light control of adventitious rooting in Arabidopsis require Argonaute. The Plant Cell Online, 17 (5):1343-1359.
doi: 10.1105/tpc.105.031625 URL |
[26] |
Sorin C, Negroni L, Balliau T, Corti H, Jacquemot M P, Davanture M, Sandberg G, Bellini Z C. 2006. Proteomic analysis of different mutant genotypes of Arabidopsis led to the identification of 11 proteins correlating with adventitious root development. Plant Physiology, 140 (1):349-364.
doi: 10.1104/pp.105.067868 URL |
[27] | Sun B, Chen L, Liu J, Zhang X, Yang Z, Liu W, Xu L. 2016. TAA family contributes to auxin production during de novo regeneration of adventitious roots from Arabidopsis leaf explants. Chinese Science Bulletin:English Version, 61 (22):4. |
[28] | Sun Yu. 2020. Effects of loading on the growth of shoot and root of Fuji apple[M. D. Dissertation]. Baoding: Hebei Agricultural University. (in Chinese) |
孙宇. 2020. 负载量对富士苹果地上部及根系生长的影响[硕士论文]. 保定: 河北农业大学. | |
[29] | Wang Qing-min, Peng Wei-xiu, Zhang Jun-pei, Pei Dong. 2006. Study on the morphological structure and hormone regulation of walnut shoots in vitro. Acta Horticulturae Sinica, 33 (2):255-259. (in Chinese) |
王清民, 彭伟秀, 张俊佩, 裴东. 2006. 核桃试管嫩茎生根的形态结构及激素调控研究. 园艺学报, 33 (2):255-259. | |
[30] | Wang Xiao-ling. 2011. Physiological and biochemical basis of adventitious roots in tetraploid locust cuttings[Ph. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese) |
王小玲. 2011. 四倍体刺槐插条不定根发生的生理生化基础研究[博士论文]. 杨凌: 西北农林科技大学. | |
[31] | Xu Nan. 2019. Factors of rooting and physiological and biochemical changes during rooting of panicle[M. D. Dissertation]. Tai’an: Shandong Agricultural University. (in Chinese) |
许楠. 2019. 影响银杏插穗生根的因子及生根过程中生理生化变化[硕士论文]. 泰安: 山东农业大学. | |
[32] | Xu Yin. 2017. Study on cutting propagation technology and rooting physiological basis of Runnan breviflora[M. D. Dissertation]. Guangzhou: South China Agricultural University. (in Chinese) |
徐茵. 2017. 短序润楠扦插繁殖技术与生根生理基础研究[硕士论文]. 广州: 华南农业大学. | |
[33] | Yan Shao-peng. 2011. Physicochemical and molecular mechanism of cutting rooting of Populus euramerican hybrids[Ph. D. Dissertation]. Harbin:Northeast Forestry University. (in Chinese) |
闫绍鹏. 2011. 欧美山杨杂种扦插生根的理化与分子机理研究[博士论文]. 哈尔滨: 东北林业大学. | |
[34] | Yu Sheng-nan, Cui Ji-zhe. 2009. The role of PIN protein in polar auxin transport. Biotechnology Bulletin,(3):20-24. (in Chinese) |
于胜楠, 崔继哲. 2009. PIN蛋白在生长素极性运输中的作用. 生物技术通报,(3):20-24. | |
[35] | Zhang Shuai. 2013. Research on propagation technology of high altitude layering of snake skin fruit[M. D. Dissertation]. Beijing: Chinese Academy of Forestry. (in Chinese) |
张帅. 2013. 蛇皮果高空压条繁殖技术的研究[硕士论文]. 北京: 中国林业科学研究院. | |
[36] |
Zhang Xueying, Li Zhongyong, Shao Jianzhu, Chen Haijiang, Xu Jizhong. 2020. A new variety of apple dwarfing rootstock‘Jizhen 2’. Acta Horticulturae Sinica, 47 (8):1625-1626. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2019-0194 |
张学英, 李中勇, 邵建柱, 陈海江, 徐继忠. 2020. 苹果矮化砧木新品种‘冀砧2号’. 园艺学报, 47 (8):1625-1626.
doi: 10.16420/j.issn.0513-353x.2019-0194 |
|
[37] |
Zheng Qingbo, Bao Zeyang, Lan Qingqing, Zhou Yuwen, Zhou Yufei, Zheng Caixia, Li Xu. 2023. Advances in studies on adventitious root formation by juvenile- and auxin-determined. Acta Horticulturae Sinica, 50 (2):441-450. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2021-1226 |
郑清波, 鲍泽洋, 蓝青青, 周钰雯, 周雨菲, 郑彩霞, 李旭. 2023. 童性与生长素对不定根发生的影响研究进展. 园艺学报, 50 (2):441-450.
doi: 10.16420/j.issn.0513-353x.2021-1226 |
[1] | 李少旋, 王芝云, 胡大刚, 朱 波, 韩明三, . 晚熟苹果新品种‘琴富 1 号’[J]. 园艺学报, 2023, 50(S1): 1-2. |
[2] | 孙燕霞, 唐 岩, 刘大亮, 赵玲玲, 张学勇, 刘学卿, Dorota Ewa Kruczynska, 程志娟, Sylwia Keller-Przybylkowicz, 宋来庆, . 早熟苹果新品种‘烟青玉’[J]. 园艺学报, 2023, 50(S1): 3-4. |
[3] | 赵国栋, 贾林光, 陈东玫, 赵同生, 张新生, 张朝红, 李春敏, 付 友. 苹果新品种‘映红’ [J]. 园艺学报, 2023, 50(S1): 5-6. |
[4] | 蔚 露, 牛自勉, 郭文龙, 林 琭, 李 全, 李志强, 王红宁, 李鸿雁 . 早熟苹果新品种‘夏露’[J]. 园艺学报, 2023, 50(S1): 7-8. |
[5] | 秦嗣军, 张阔, 齐边斌, 于波, 吕德国. 外源碳对苹果根区土壤活性有机碳及植株生长的影响[J]. 园艺学报, 2023, 50(6): 1295-1304. |
[6] | 坎智勇, 张德辉, 李中兴, 余思思, 钱谦, 樊天乐, 李雪薇, 马锋旺, 管清美. 90个苹果品种耐寒性评价和全基因组关联分析[J]. 园艺学报, 2023, 50(5): 921-932. |
[7] | 张琨, 思彬彬, 周军, 任玉锋, 张欣, 徐文娣, 王佳伟, 乔帅, 王惠冉. 苹果砧木‘青砧1号’叶片cDNA文库构建及MdMLO上游调控因子的筛选[J]. 园艺学报, 2023, 50(5): 933-946. |
[8] | 刘柚藓, 李国防, 檀鸣, 杨志昌, 周世伟, 霍文静, 张鹤, 孙建设, 邵建柱. 苹果MdTOPP13/28在腋芽萌发中的表达分析[J]. 园艺学报, 2023, 50(4): 697-712. |
[9] | 宁源生, 李欢, 宋建飞, 于婷婷, 韩梦圆, 彭璐琳, 贾竣淇, 张玮玮, 杨洪强. 苹果NCL家族基因与根系细胞钙离子浓度变化的关系[J]. 园艺学报, 2023, 50(3): 475-484. |
[10] | 于婷婷, 李欢, 宁源生, 宋建飞, 彭璐琳, 贾竣淇, 张玮玮, 杨洪强. 苹果GRAS全基因组鉴定及其对生长素的响应分析[J]. 园艺学报, 2023, 50(2): 397-409. |
[11] | 袁馨, 徐云鹤, 张雨培, 单楠, 陈楚英, 万春鹏, 开文斌, 翟夏琬, 陈金印, 甘增宇. 猕猴桃后熟过程中ABA响应结合因子AcAREB1调控AcGH3.1的表达[J]. 园艺学报, 2023, 50(1): 53-64. |
[12] | 韩晓蕾, 张彩霞, 刘 锴, 杨 安, 严家帝, 李武兴, 康立群, 丛佩华. 中熟苹果新品种‘中苹优蕾’[J]. 园艺学报, 2022, 49(S2): 1-2. |
[13] | 张晓明, 闫国华, 周 宇, 王 晶, 段续伟, 吴传宝, 张开春. 甜樱桃砧木新品种‘京春2号’[J]. 园艺学报, 2022, 49(S2): 31-32. |
[14] | 田红梅, 刘 娟, 张长坤, 陶 珍, 张 建, 王朋成, . 甜瓜砧木用南瓜新品种‘皖砧6号’[J]. 园艺学报, 2022, 49(S2): 127-128. |
[15] | 韩晓蕾, 张彩霞, 刘 锴, 严家帝, 李武兴, 康立群, 丛佩华. 中熟苹果新品种‘苹优2号’[J]. 园艺学报, 2022, 49(S1): 1-2. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||
版权所有 © 2012 《园艺学报》编辑部 京ICP备10030308号-2 国际联网备案号 11010802023439
编辑部地址: 北京市海淀区中关村南大街12号中国农业科学院蔬菜花卉研究所 邮编: 100081
电话: 010-82109523 E-Mail: yuanyixuebao@126.com
技术支持:北京玛格泰克科技发展有限公司