脱毒骏枣幼树叶片活性氧代谢的研究

园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 969-973.

脱毒骏枣幼树叶片活性氧代谢的研究

刘和;郭艳^*;宋金龙

(山西农业大学园艺学院, 山西太谷030801)

收稿日期:2006-02-28 修回日期:2006-07-06 出版日期:2006-10-25 发布日期:2006-10-25

Studies on Metabolism of Active Oxygen Spec ies in the Leaves of YoungVirus-free Trees of Ziziphus jujuba‘Junzao’

Liu He;Guo Yan;Song Jinlong

(College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi 030801, China)

Received:2006-02-28 Revised:2006-07-06 Online:2006-10-25 Published:2006-10-25

摘要/Abstract

摘要： 大田自然生长条件下脱毒骏枣幼树叶片的超氧阴离子(O₂·) 生成速率、过氧化氢(H₂O₂ ) 、
丙二醛(MDA) 、还原型谷胱甘肽(GSH) 、抗坏血酸(AsA) 含量以及超氧化物歧化酶( SOD) 、过氧化物酶( POD) 、过氧化氢酶(CAT) 、谷胱甘肽还原酶(GR) 、抗坏血酸过氧化物酶(APX) 活性的变化规律与对照未脱毒骏枣幼树叶片相应各指标的变化规律基本一致。生长前期脱毒骏枣幼树叶片的O₂·生成速率、H₂O₂、MDA含量以及SOD、POD、CAT、GR、APX活性都低于对照, 并且随着树体的不断生长, 差异越来越明显。后期除O₂· 生成速率、H₂O₂、MDA含量仍显著低于对照外, 其它各指标均显著高于对照。脱毒幼树叶片的AsA含量在整个生长期都高于对照, 前期差异不显著, 中后期差异显著。试验结果表明,脱毒骏枣与未脱毒骏枣相比具有较低的活性氧水平和较强的活性氧清除能力, 在生长后期表现出较强的抗性。

关键词: 枣, 脱毒, 活性氧代谢, 保护酶

Abstract: The dynamics of the generation rate of O₂·, the contents of H₂O₂, MDA, GSH, AsA and the activities of SOD, POD, CAT, GR, APX in the leaves of virus-free Ziziphus jujuba Mill. ‘Junzao’trees were similiar with those in leaves of common‘Junzao’trees. The generation rate of O₂·, the contents of H₂O₂, MDA, GSH, AsA and activities of SOD, POD, CAT, GR, APX in the leaves of virus-free‘Junzao’trees were lower than those of common‘Junzao’trees in the early period of growth. The differences became significant with the growth of trees. In the later period of growth, most indexs in the leaves of virus-free
‘Junzao’treeswere higher than those of common‘Junzao’trees except the generation rate of O₂·, the concents of H₂O₂and MDA. However, the content of AsA in the leaves of virus-free‘Junzao’trees was higher than that of common‘Junzao’trees throughout the growth period, and the difference was significant only in later period of growth. The results showed that there were lower reactive oxygen species (ROS) content and stronger ability to clean out the ROS in virus-free‘Junzao’trees than those in common 'Junzao' tree.

Key words: Ziziphus jujuba, Virus-free, ROS metabolism, Protective enzyme

刘和;郭艳;宋金龙. 脱毒骏枣幼树叶片活性氧代谢的研究[J]. 园艺学报, 2006, 33(5): 969-973.

Liu He;Guo Yan;Song Jinlong. Studies on Metabolism of Active Oxygen Spec ies in the Leaves of YoungVirus-free Trees of Ziziphus jujuba‘Junzao’[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 969-973.

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