园艺学报 ›› 2022, Vol. 49 ›› Issue (10): 2273-2284.doi: 10.16420/j.issn.0513-353x.2021-0869
赵立民, 李嘉伟, 张飞, 苏江硕, 房伟民, 王海滨, 蒋甲福, 陈素梅, 陈发棣, 管志勇()
收稿日期:
2021-12-10
修回日期:
2022-07-31
出版日期:
2022-10-25
发布日期:
2022-10-31
通讯作者:
管志勇
E-mail:guanzhy@njau.edu.cn
基金资助:
ZHAO Limin, LI Jiawei, ZHANG Fei, SU Jiangshuo, FANG Weimin, WANG Haibin, JIANG Jiafu, CHEN Sumei, CHEN Fadi, and GUAN Zhiyong()
Received:
2021-12-10
Revised:
2022-07-31
Online:
2022-10-25
Published:
2022-10-31
Contact:
and GUAN Zhiyong
E-mail:guanzhy@njau.edu.cn
摘要:
以“中国菊花种质资源保存中心”保存的950个切花小菊(Chrysanthemum morifolium)品种的13个表型性状为基本数据,依据舌状花花色分为8组,采用系统聚类法进行聚类分析。基于2种遗传距离、4种聚类方法和5种总体取样规模构建了40个候选切花小菊核心种质,并利用4个评价指标比较构建效果。利用筛选得到的最佳构建方案构建初级核心种质。以6个特征值和5个评价参数综合评价所构建核心种质,以检验核心种质有效性和代表性。结果表明,采用标准化欧氏距离、离差平方和法、10%总体取样比例的备选核心种质综合表现最佳。最终构建获得包含95个切花小菊品种的初级核心种质,占原种质10%。核心种质评价表明初级核心种质构建有效且质量较高,能够在保证冗余较少的情况下充分代表原种质遗传多样性。
中图分类号:
赵立民, 李嘉伟, 张飞, 苏江硕, 房伟民, 王海滨, 蒋甲福, 陈素梅, 陈发棣, 管志勇. 基于表型数据构建切花小菊核心种质[J]. 园艺学报, 2022, 49(10): 2273-2284.
ZHAO Limin, LI Jiawei, ZHANG Fei, SU Jiangshuo, FANG Weimin, WANG Haibin, JIANG Jiafu, CHEN Sumei, CHEN Fadi, and GUAN Zhiyong. Construction of a Core Collection of Spray Cut Chrysanthemum Based on Phenotypic Data[J]. Acta Horticulturae Sinica, 2022, 49(10): 2273-2284.
性状Trait | 赋值Assigning value |
---|---|
瓣型Petal type | 平瓣 = 1,内曲 = 2, 匙瓣 = 3,管瓣 = 4,漏斗状 = 5 Flat petal = 1,Involute petal = 2,Spoon petal = 3,Tube petal = 4,Funnel petal = 5 |
舌状花色 Color of ligulate flower | 黄 = 1,白 = 2,粉 = 3,红 = 4,紫 = 5,橙 = 6,绿 = 7,双色 = 8 Yellow = 1,White = 2,Pink = 3,Red = 4,Purple = 5,Orange = 6,Green = 7,Two colors = 8 |
叶型Leaf type | 正叶 = 1,深刻正叶 = 2,长叶 = 3,深刻长叶 = 4,圆叶 = 5,蓬叶 = 6,葵叶 = 7,反转叶 = 8 Positive leaf = 1,Deep positive leaf = 2,Long leaf = 3,Deep long leaf = 4,Round leaf = 5,Fluffy leaf = 6,Sunflower leaf = 7,Reverse leaf = 8 |
花期Florescence | 上(10.20以前花瓣显色)= 1,中(10.20至11.10之间花瓣显色)= 2,下(11.10以后花瓣显色)= 3 Petal color development before October 20 = 1,petal color development from October 20 to November 10 = 2,petal color development after November 10 = 3 |
花型Flower type | 单瓣 = 1,复瓣 = 2,托桂 = 3,风车 = 4,蜂窝 = 5,管盘 = 6,单管 = 7,莲座 = 8,芍药 = 9 Single petal = 1,Double petal = 2,Laurel petal = 3,Windmill petal = 4,Honeycomb petal = 5,Tubesheet petal = 6,Tube petal = 7,Lotus petal = 8,Peony petal = 9 |
表1 菊花 5 个质量性状分级与赋值
Table 1 Coden designation for 5 qualitative traits in Chrysanthemum morifolium
性状Trait | 赋值Assigning value |
---|---|
瓣型Petal type | 平瓣 = 1,内曲 = 2, 匙瓣 = 3,管瓣 = 4,漏斗状 = 5 Flat petal = 1,Involute petal = 2,Spoon petal = 3,Tube petal = 4,Funnel petal = 5 |
舌状花色 Color of ligulate flower | 黄 = 1,白 = 2,粉 = 3,红 = 4,紫 = 5,橙 = 6,绿 = 7,双色 = 8 Yellow = 1,White = 2,Pink = 3,Red = 4,Purple = 5,Orange = 6,Green = 7,Two colors = 8 |
叶型Leaf type | 正叶 = 1,深刻正叶 = 2,长叶 = 3,深刻长叶 = 4,圆叶 = 5,蓬叶 = 6,葵叶 = 7,反转叶 = 8 Positive leaf = 1,Deep positive leaf = 2,Long leaf = 3,Deep long leaf = 4,Round leaf = 5,Fluffy leaf = 6,Sunflower leaf = 7,Reverse leaf = 8 |
花期Florescence | 上(10.20以前花瓣显色)= 1,中(10.20至11.10之间花瓣显色)= 2,下(11.10以后花瓣显色)= 3 Petal color development before October 20 = 1,petal color development from October 20 to November 10 = 2,petal color development after November 10 = 3 |
花型Flower type | 单瓣 = 1,复瓣 = 2,托桂 = 3,风车 = 4,蜂窝 = 5,管盘 = 6,单管 = 7,莲座 = 8,芍药 = 9 Single petal = 1,Double petal = 2,Laurel petal = 3,Windmill petal = 4,Honeycomb petal = 5,Tubesheet petal = 6,Tube petal = 7,Lotus petal = 8,Peony petal = 9 |
种质 Collection | 总体取样规模/% Overall sampling size | 组内比例/% Ratio in group | |||||||
---|---|---|---|---|---|---|---|---|---|
Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | Ⅵ | Ⅶ | Ⅷ | ||
核心种质 Core collection | 5 | 14.89 | 12.77 | 12.77 | 12.77 | 12.77 | 12.77 | 8.51 | 12.77 |
10 | 13.68 | 13.68 | 13.68 | 12.63 | 12.63 | 11.58 | 9.47 | 12.63 | |
15 | 14.08 | 13.38 | 13.38 | 11.97 | 12.68 | 11.97 | 9.15 | 13.38 | |
20 | 13.90 | 13.37 | 13.37 | 12.30 | 12.83 | 11.76 | 9.09 | 13.37 | |
25 | 13.87 | 13.45 | 13.45 | 12.18 | 13.03 | 11.76 | 9.24 | 13.03 | |
原始种质 Original collection | 19.68 | 15.16 | 16.63 | 10.21 | 12.84 | 8.11 | 3.16 | 14.21 |
表2 对数比例策略在不同总体取样规模下的各组取样比例
Table 2 Sampling proportion of each group in different overall sampling size of logarithmic proportion strategy
种质 Collection | 总体取样规模/% Overall sampling size | 组内比例/% Ratio in group | |||||||
---|---|---|---|---|---|---|---|---|---|
Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | Ⅵ | Ⅶ | Ⅷ | ||
核心种质 Core collection | 5 | 14.89 | 12.77 | 12.77 | 12.77 | 12.77 | 12.77 | 8.51 | 12.77 |
10 | 13.68 | 13.68 | 13.68 | 12.63 | 12.63 | 11.58 | 9.47 | 12.63 | |
15 | 14.08 | 13.38 | 13.38 | 11.97 | 12.68 | 11.97 | 9.15 | 13.38 | |
20 | 13.90 | 13.37 | 13.37 | 12.30 | 12.83 | 11.76 | 9.09 | 13.37 | |
25 | 13.87 | 13.45 | 13.45 | 12.18 | 13.03 | 11.76 | 9.24 | 13.03 | |
原始种质 Original collection | 19.68 | 15.16 | 16.63 | 10.21 | 12.84 | 8.11 | 3.16 | 14.21 |
遗传距离 Genetic distance | 聚类方法 Cluster method | 总体取样比例/% Sampling proportion | 备选核心种质 Core collection | I | VR/% | RPR/% | CR/% |
---|---|---|---|---|---|---|---|
标准化欧氏距离(D1) Standard Euclidean | 离差平方和法(C1) Ward's method | 5 | D1C1-5 | 1.798 | 127.98 | 95.37 | 99.71 |
10 | D1C1-10 | 1.817 | 122.37 | 100.00 | 99.96 | ||
15 | D1C1-15 | 1.796 | 117.05 | 100.00 | 99.96 | ||
20 | D1C1-20 | 1.786 | 114.70 | 100.00 | 99.96 | ||
25 | D1C1-25 | 1.772 | 112.95 | 100.00 | 99.96 | ||
最长距离法(C2) Complete linkage | 5 | D1C2-5 | 1.807 | 123.68 | 96.30 | 96.37 | |
10 | D1C2-10 | 1.808 | 120.11 | 99.07 | 99.96 | ||
15 | D1C2-15 | 1.800 | 116.53 | 100.00 | 99.96 | ||
20 | D1C2-20 | 1.787 | 115.76 | 100.00 | 99.96 | ||
25 | D1C2-25 | 1.768 | 112.58 | 100.00 | 99.96 | ||
最短距离法(C3) Single linkage | 5 | D1C3-5 | 1.803 | 127.49 | 94.44 | 98.50 | |
10 | D1C3-10 | 1.813 | 121.41 | 99.07 | 99.71 | ||
15 | D1C3-15 | 1.815 | 118.75 | 100.00 | 99.96 | ||
20 | D1C3-20 | 1.799 | 115.08 | 100.00 | 99.96 | ||
25 | D1C3-25 | 1.793 | 113.06 | 100.00 | 99.96 | ||
非加权组平均法(C4) UPGMA | 5 | D1C4-5 | 1.813 | 126.06 | 96.30 | 99.69 | |
10 | D1C4-10 | 1.808 | 120.77 | 100.00 | 99.96 | ||
15 | D1C4-15 | 1.806 | 116.52 | 100.00 | 99.95 | ||
20 | D1C4-20 | 1.789 | 114.27 | 100.00 | 99.95 | ||
25 | D1C4-25 | 1.785 | 114.35 | 100.00 | 100.00 | ||
马氏距离(D2) Mahalanobis | 离差平方和法(C1) Ward's method | 5 | D2C1-5 | 1.779 | 122.63 | 95.37 | 95.95 |
10 | D2C1-10 | 1.805 | 115.58 | 98.15 | 97.41 | ||
15 | D2C1-15 | 1.799 | 114.42 | 100.00 | 99.61 | ||
20 | D2C1-20 | 1.784 | 112.64 | 100.00 | 99.61 | ||
25 | D2C1-25 | 1.763 | 110.84 | 100.00 | 99.61 | ||
最长距离法(C2) Complete linkage | 5 | D2C2-5 | 1.765 | 119.40 | 95.37 | 95.42 | |
10 | D2C2-10 | 1.797 | 118.81 | 100.00 | 99.61 | ||
15 | D2C2-15 | 1.802 | 115.75 | 100.00 | 99.61 | ||
20 | D2C2-20 | 1.790 | 114.29 | 100.00 | 99.94 | ||
25 | D2C2-25 | 1.768 | 111.81 | 100.00 | 99.94 | ||
最短距离法(C3) Single linkage | 5 | D2C3-5 | 1.770 | 121.99 | 95.37 | 94.17 | |
10 | D2C3-10 | 1.800 | 120.02 | 100.00 | 99.68 | ||
15 | D2C3-15 | 1.801 | 116.42 | 100.00 | 99.96 | ||
20 | D2C3-20 | 1.793 | 114.28 | 100.00 | 99.96 | ||
25 | D2C3-25 | 1.786 | 112.51 | 100.00 | 100.00 | ||
非加权组平均法(C4) UPGMA | 5 | D2C4-5 | 1.772 | 123.64 | 95.37 | 93.59 | |
10 | D2C4-10 | 1.810 | 119.68 | 100.00 | 99.96 | ||
15 | D2C4-15 | 1.800 | 116.14 | 100.00 | 99.96 | ||
20 | D2C4-20 | 1.797 | 113.88 | 100.00 | 99.96 | ||
25 | D2C4-25 | 1.783 | 112.12 | 100.00 | 100.00 |
表3 不同构建策略的比较
Table 3 The comparison of different construction strategy
遗传距离 Genetic distance | 聚类方法 Cluster method | 总体取样比例/% Sampling proportion | 备选核心种质 Core collection | I | VR/% | RPR/% | CR/% |
---|---|---|---|---|---|---|---|
标准化欧氏距离(D1) Standard Euclidean | 离差平方和法(C1) Ward's method | 5 | D1C1-5 | 1.798 | 127.98 | 95.37 | 99.71 |
10 | D1C1-10 | 1.817 | 122.37 | 100.00 | 99.96 | ||
15 | D1C1-15 | 1.796 | 117.05 | 100.00 | 99.96 | ||
20 | D1C1-20 | 1.786 | 114.70 | 100.00 | 99.96 | ||
25 | D1C1-25 | 1.772 | 112.95 | 100.00 | 99.96 | ||
最长距离法(C2) Complete linkage | 5 | D1C2-5 | 1.807 | 123.68 | 96.30 | 96.37 | |
10 | D1C2-10 | 1.808 | 120.11 | 99.07 | 99.96 | ||
15 | D1C2-15 | 1.800 | 116.53 | 100.00 | 99.96 | ||
20 | D1C2-20 | 1.787 | 115.76 | 100.00 | 99.96 | ||
25 | D1C2-25 | 1.768 | 112.58 | 100.00 | 99.96 | ||
最短距离法(C3) Single linkage | 5 | D1C3-5 | 1.803 | 127.49 | 94.44 | 98.50 | |
10 | D1C3-10 | 1.813 | 121.41 | 99.07 | 99.71 | ||
15 | D1C3-15 | 1.815 | 118.75 | 100.00 | 99.96 | ||
20 | D1C3-20 | 1.799 | 115.08 | 100.00 | 99.96 | ||
25 | D1C3-25 | 1.793 | 113.06 | 100.00 | 99.96 | ||
非加权组平均法(C4) UPGMA | 5 | D1C4-5 | 1.813 | 126.06 | 96.30 | 99.69 | |
10 | D1C4-10 | 1.808 | 120.77 | 100.00 | 99.96 | ||
15 | D1C4-15 | 1.806 | 116.52 | 100.00 | 99.95 | ||
20 | D1C4-20 | 1.789 | 114.27 | 100.00 | 99.95 | ||
25 | D1C4-25 | 1.785 | 114.35 | 100.00 | 100.00 | ||
马氏距离(D2) Mahalanobis | 离差平方和法(C1) Ward's method | 5 | D2C1-5 | 1.779 | 122.63 | 95.37 | 95.95 |
10 | D2C1-10 | 1.805 | 115.58 | 98.15 | 97.41 | ||
15 | D2C1-15 | 1.799 | 114.42 | 100.00 | 99.61 | ||
20 | D2C1-20 | 1.784 | 112.64 | 100.00 | 99.61 | ||
25 | D2C1-25 | 1.763 | 110.84 | 100.00 | 99.61 | ||
最长距离法(C2) Complete linkage | 5 | D2C2-5 | 1.765 | 119.40 | 95.37 | 95.42 | |
10 | D2C2-10 | 1.797 | 118.81 | 100.00 | 99.61 | ||
15 | D2C2-15 | 1.802 | 115.75 | 100.00 | 99.61 | ||
20 | D2C2-20 | 1.790 | 114.29 | 100.00 | 99.94 | ||
25 | D2C2-25 | 1.768 | 111.81 | 100.00 | 99.94 | ||
最短距离法(C3) Single linkage | 5 | D2C3-5 | 1.770 | 121.99 | 95.37 | 94.17 | |
10 | D2C3-10 | 1.800 | 120.02 | 100.00 | 99.68 | ||
15 | D2C3-15 | 1.801 | 116.42 | 100.00 | 99.96 | ||
20 | D2C3-20 | 1.793 | 114.28 | 100.00 | 99.96 | ||
25 | D2C3-25 | 1.786 | 112.51 | 100.00 | 100.00 | ||
非加权组平均法(C4) UPGMA | 5 | D2C4-5 | 1.772 | 123.64 | 95.37 | 93.59 | |
10 | D2C4-10 | 1.810 | 119.68 | 100.00 | 99.96 | ||
15 | D2C4-15 | 1.800 | 116.14 | 100.00 | 99.96 | ||
20 | D2C4-20 | 1.797 | 113.88 | 100.00 | 99.96 | ||
25 | D2C4-25 | 1.783 | 112.12 | 100.00 | 100.00 |
性状Trait | 原始种质Original collection | 核心种质 Core collection | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
最小值 Min | 最大值 Max | 均值 Mean | 标准偏差 SD | 变异系数/%CV | 遗传多样性指数I | 最小值 Min | 最大值 Max | 均值 Mean | 标准偏差SD | 变异系数/%CV | 遗传多样性指数I | ||
花期Florescence | 1 | 3 | 2.13 | 0.45 | 0.21 | 0.63 | 1 | 3 | 2.17 | 0.60 | 0.27 | 0.89 | |
叶型Leaf type | 1 | 8 | 2.98 | 0.95 | 0.32 | 0.85 | 1 | 8 | 3.29 | 1.52 | 0.46 | 1.34 | |
瓣型Petal type | 1 | 4 | 1.70 | 1.08 | 0.63 | 0.90 | 1 | 4 | 1.92 | 1.20 | 0.63 | 1.13 | |
花型Flower type | 1 | 9 | 2.02 | 1.86 | 0.92 | 1.22 | 1 | 9 | 2.93 | 2.46 | 0.84 | 1.64 | |
花色Color of ligulate flower | 1 | 8 | 3.89 | 2.36 | 0.61 | 1.99 | 1 | 8 | 4.33 | 2.31 | 0.53 | 2.07 | |
舌状花数 Number of ligulate flowers | 11 | 608 | 76.53 | 96.91 | 1.27 | 1.40 | 11 | 608 | 122.33 | 131.30 | 1.07 | 1.65 | |
株高/cm Plant height | 15 | 189 | 93.70 | 24.45 | 0.26 | 2.09 | 15 | 189 | 90.69 | 29.44 | 0.32 | 2.14 | |
叶宽/cm Leaf width(LW) | 2.70 | 11.33 | 5.88 | 1.24 | 0.21 | 2.07 | 2.75 | 11.33 | 5.92 | 1.61 | 0.27 | 2.19 | |
叶长/cm Leaf length(LL) | 4.50 | 18.70 | 10.80 | 2.37 | 0.22 | 2.06 | 4.50 | 18.70 | 10.81 | 2.79 | 0.26 | 2.15 | |
叶长宽比LL/LW | 1.16 | 2.55 | 1.78 | 0.21 | 0.12 | 2.06 | 1.16 | 2.55 | 1.77 | 0.28 | 0.16 | 2.19 | |
外层瓣长/cm Outer petal length | 0.54 | 7.79 | 2.57 | 0.91 | 0.35 | 2.06 | 0.54 | 7.79 | 2.66 | 1.34 | 0.50 | 2.12 | |
花心直径/cm Flower center diamete | 0 | 5.1 | 1.36 | 0.72 | 0.53 | 1.86 | 0 | 5.1 | 1.23 | 1.07 | 0.87 | 1.93 | |
花序直径/cm Inflorescence diameter | 1.56 | 17.24 | 5.08 | 1.61 | 0.32 | 2.07 | 1.56 | 17.24 | 5.40 | 2.42 | 0.45 | 2.18 |
表4 切花小菊原始种质与核心种质性状特征值比较
Table 4 Comparison of agronomic characters between Original collection and Core collection of cut Chrysanthemum morifolium
性状Trait | 原始种质Original collection | 核心种质 Core collection | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
最小值 Min | 最大值 Max | 均值 Mean | 标准偏差 SD | 变异系数/%CV | 遗传多样性指数I | 最小值 Min | 最大值 Max | 均值 Mean | 标准偏差SD | 变异系数/%CV | 遗传多样性指数I | ||
花期Florescence | 1 | 3 | 2.13 | 0.45 | 0.21 | 0.63 | 1 | 3 | 2.17 | 0.60 | 0.27 | 0.89 | |
叶型Leaf type | 1 | 8 | 2.98 | 0.95 | 0.32 | 0.85 | 1 | 8 | 3.29 | 1.52 | 0.46 | 1.34 | |
瓣型Petal type | 1 | 4 | 1.70 | 1.08 | 0.63 | 0.90 | 1 | 4 | 1.92 | 1.20 | 0.63 | 1.13 | |
花型Flower type | 1 | 9 | 2.02 | 1.86 | 0.92 | 1.22 | 1 | 9 | 2.93 | 2.46 | 0.84 | 1.64 | |
花色Color of ligulate flower | 1 | 8 | 3.89 | 2.36 | 0.61 | 1.99 | 1 | 8 | 4.33 | 2.31 | 0.53 | 2.07 | |
舌状花数 Number of ligulate flowers | 11 | 608 | 76.53 | 96.91 | 1.27 | 1.40 | 11 | 608 | 122.33 | 131.30 | 1.07 | 1.65 | |
株高/cm Plant height | 15 | 189 | 93.70 | 24.45 | 0.26 | 2.09 | 15 | 189 | 90.69 | 29.44 | 0.32 | 2.14 | |
叶宽/cm Leaf width(LW) | 2.70 | 11.33 | 5.88 | 1.24 | 0.21 | 2.07 | 2.75 | 11.33 | 5.92 | 1.61 | 0.27 | 2.19 | |
叶长/cm Leaf length(LL) | 4.50 | 18.70 | 10.80 | 2.37 | 0.22 | 2.06 | 4.50 | 18.70 | 10.81 | 2.79 | 0.26 | 2.15 | |
叶长宽比LL/LW | 1.16 | 2.55 | 1.78 | 0.21 | 0.12 | 2.06 | 1.16 | 2.55 | 1.77 | 0.28 | 0.16 | 2.19 | |
外层瓣长/cm Outer petal length | 0.54 | 7.79 | 2.57 | 0.91 | 0.35 | 2.06 | 0.54 | 7.79 | 2.66 | 1.34 | 0.50 | 2.12 | |
花心直径/cm Flower center diamete | 0 | 5.1 | 1.36 | 0.72 | 0.53 | 1.86 | 0 | 5.1 | 1.23 | 1.07 | 0.87 | 1.93 | |
花序直径/cm Inflorescence diameter | 1.56 | 17.24 | 5.08 | 1.61 | 0.32 | 2.07 | 1.56 | 17.24 | 5.40 | 2.42 | 0.45 | 2.18 |
性状Trait | 极差符合率CR/% | 变异系数变化率VR/% | 表型保留比例RPR/% | Pt | PF |
---|---|---|---|---|---|
花期Florescence | 100.00 | 131.08 | 100.00 | 0.570 | 0.472 |
叶型Leaf type | 100.00 | 144.45 | 100.00 | 0.053 | 0.005* |
瓣型Petal type | 100.00 | 98.64 | 100.00 | 0.096 | 0.068 |
花型Flower type | 100.00 | 91.10 | 100.00 | 0.001* | 0.000* |
花色Color of ligulate flower | 100.00 | 88.19 | 100.00 | 0.085 | 0.088 |
舌状花数Number of ligulate flowers | 100.00 | 84.75 | 100.00 | 0.001* | 0.000* |
株高/cm Plant height | 100.00 | 124.39 | 100.00 | 0.338 | 0.263 |
叶宽/cm Leaf width(LW) | 99.42 | 129.12 | 100.00 | 0.844 | 0.807 |
叶长/cm Leaf length(LL) | 100.00 | 117.56 | 100.00 | 0.967 | 0.962 |
叶长宽比LL/LW | 100.00 | 133.54 | 100.00 | 0.702 | 0.629 |
外层瓣长/cm Outer petal length | 100.00 | 142.64 | 100.00 | 0.542 | 0.403 |
花心直径/cm Flower center diamete | 100.00 | 163.87 | 100.00 | 0.248 | 0.111 |
花序直径/cm Inflorescence diameter | 100.00 | 141.34 | 100.00 | 0.216 | 0.084 |
表5 核心种质与原始种质各性状评价参数和t检验、F检验
Table 5 The evaluation parameters,t-test and F-test of traits of core collection and original collection
性状Trait | 极差符合率CR/% | 变异系数变化率VR/% | 表型保留比例RPR/% | Pt | PF |
---|---|---|---|---|---|
花期Florescence | 100.00 | 131.08 | 100.00 | 0.570 | 0.472 |
叶型Leaf type | 100.00 | 144.45 | 100.00 | 0.053 | 0.005* |
瓣型Petal type | 100.00 | 98.64 | 100.00 | 0.096 | 0.068 |
花型Flower type | 100.00 | 91.10 | 100.00 | 0.001* | 0.000* |
花色Color of ligulate flower | 100.00 | 88.19 | 100.00 | 0.085 | 0.088 |
舌状花数Number of ligulate flowers | 100.00 | 84.75 | 100.00 | 0.001* | 0.000* |
株高/cm Plant height | 100.00 | 124.39 | 100.00 | 0.338 | 0.263 |
叶宽/cm Leaf width(LW) | 99.42 | 129.12 | 100.00 | 0.844 | 0.807 |
叶长/cm Leaf length(LL) | 100.00 | 117.56 | 100.00 | 0.967 | 0.962 |
叶长宽比LL/LW | 100.00 | 133.54 | 100.00 | 0.702 | 0.629 |
外层瓣长/cm Outer petal length | 100.00 | 142.64 | 100.00 | 0.542 | 0.403 |
花心直径/cm Flower center diamete | 100.00 | 163.87 | 100.00 | 0.248 | 0.111 |
花序直径/cm Inflorescence diameter | 100.00 | 141.34 | 100.00 | 0.216 | 0.084 |
序号No. | 品种名称 Variety denomination | 序号 No. | 品种名称 Variety denomination | 序号No. | 品种名称 Variety denomination |
---|---|---|---|---|---|
1 | Q12-47-3 | 33 | QD3-101 | 65 | Q12-36-3 |
2 | 南农月光Nannong Yueguang | 34 | Q12-18-3 | 66 | 南农花妆Nannong Huazhuang |
3 | Q11-1-18 | 35 | 南农红羽Nannong Hongyu | 67 | 南农锦风车Nannong Jinfengche |
4 | 南农金太阳Nannong Jintaiyang | 36 | Qx-066 | 68 | 南农紫唇Nannong Zichun |
5 | 南农玉泉Nannong Yuquan | 37 | Q12-41-1 | 69 | 憩风车Qifengche |
6 | 南农草莓Nannong Caomei | 38 | Q12-49-1 | 70 | 南农紫云间Nannong Ziyunjian |
7 | Q05-6 | 39 | 南农红桂Nannong Honggui | 71 | Q05-8 |
8 | 南农粉乒乓Nannong Fenpingpang | 40 | 南农艳秋Nannong Yanqiu | 72 | 南农金盏Nannong Jinzhan |
9 | 南农赤风车Nannong Chifengche | 41 | Qx-150 | 73 | Qx-073 |
10 | 南农橙乒Nannong Chengpingpang | 42 | Q04-52 | 74 | 南农晨霞Nannong Chenxia |
11 | Q01-101 | 43 | Q06-13 | 75 | 南农紫珠Nannong Zizhu |
12 | 南农丽月Nannong Liyue | 44 | 南农玫丽Nannong Meili | 76 | 南农桃柳Nannong Taoliu |
13 | Q04-32 | 45 | Q11-45-8 | 77 | Q04-38 |
14 | Qx-017 | 46 | Q11-13-3 | 78 | Qx-020 |
15 | Q12-43-1 | 47 | Q10-2-2 | 79 | 南农裂帛Nannong Liebo |
16 | Q02-4 | 48 | Q12-21-1 | 80 | 南农红妆Nannong Hongzhuang |
17 | Q04-1 | 49 | Q04-36 | 81 | 金松月Jinsongyue |
18 | Q05-7 | 50 | 南农紫芍药Nannong Zishaoyao | 82 | 南农一点莓Nannong Yidianmei |
19 | 南农香金针Nannong Xiangjinzhen | 51 | Q12-38-7 | 83 | Qx-013 |
20 | Q12-17-3 | 52 | Q11-24-56 | 84 | Qx-038 |
21 | Q06-8 | 53 | Q01-7 | 85 | 南农小草莓Nannong Xiaocaomei |
22 | Q16-8 | 54 | 南农香橙点Nannong Xiangchengdian | 86 | 南农紫星Nannong Zixing |
23 | Q11-11-5 | 55 | Q12-6-3 | 87 | Q04-56 |
24 | Q11-11-4 | 56 | Q01-51 | 88 | 球早Qiuzao |
25 | Q03-9 | 57 | 绿安娜Lüanna | 89 | 南农粉丽Nannong Fenli |
26 | 南农白蝴蝶Nannong Baihudie | 58 | QD3-170 | 90 | Qx-161 |
27 | 南农庐月Nannong Luyue | 59 | Q03-7 | 91 | 南农旋绿Nannong Xuanlü |
28 | Q06-42 | 60 | 南农瑗绿Nannong Ailü | 92 | 南农极点Nannong Jidian |
29 | Q01-70 | 61 | Qx-036 | 93 | Q01-38 |
30 | Q12-9-1 | 62 | Q06-3 | 94 | Q01-58 |
31 | 南农粉樱Nannong Fenying | 63 | 南农彩霞Nannong Caixia | 95 | 南农茶语Nannong Chayu |
32 | 南农佳丽Nannong Jiali | 64 | Q12-5-4 |
表6 切花小菊初级核心种质品种名录
Table 6 A list of primary core collection of cut chrysanthemum
序号No. | 品种名称 Variety denomination | 序号 No. | 品种名称 Variety denomination | 序号No. | 品种名称 Variety denomination |
---|---|---|---|---|---|
1 | Q12-47-3 | 33 | QD3-101 | 65 | Q12-36-3 |
2 | 南农月光Nannong Yueguang | 34 | Q12-18-3 | 66 | 南农花妆Nannong Huazhuang |
3 | Q11-1-18 | 35 | 南农红羽Nannong Hongyu | 67 | 南农锦风车Nannong Jinfengche |
4 | 南农金太阳Nannong Jintaiyang | 36 | Qx-066 | 68 | 南农紫唇Nannong Zichun |
5 | 南农玉泉Nannong Yuquan | 37 | Q12-41-1 | 69 | 憩风车Qifengche |
6 | 南农草莓Nannong Caomei | 38 | Q12-49-1 | 70 | 南农紫云间Nannong Ziyunjian |
7 | Q05-6 | 39 | 南农红桂Nannong Honggui | 71 | Q05-8 |
8 | 南农粉乒乓Nannong Fenpingpang | 40 | 南农艳秋Nannong Yanqiu | 72 | 南农金盏Nannong Jinzhan |
9 | 南农赤风车Nannong Chifengche | 41 | Qx-150 | 73 | Qx-073 |
10 | 南农橙乒Nannong Chengpingpang | 42 | Q04-52 | 74 | 南农晨霞Nannong Chenxia |
11 | Q01-101 | 43 | Q06-13 | 75 | 南农紫珠Nannong Zizhu |
12 | 南农丽月Nannong Liyue | 44 | 南农玫丽Nannong Meili | 76 | 南农桃柳Nannong Taoliu |
13 | Q04-32 | 45 | Q11-45-8 | 77 | Q04-38 |
14 | Qx-017 | 46 | Q11-13-3 | 78 | Qx-020 |
15 | Q12-43-1 | 47 | Q10-2-2 | 79 | 南农裂帛Nannong Liebo |
16 | Q02-4 | 48 | Q12-21-1 | 80 | 南农红妆Nannong Hongzhuang |
17 | Q04-1 | 49 | Q04-36 | 81 | 金松月Jinsongyue |
18 | Q05-7 | 50 | 南农紫芍药Nannong Zishaoyao | 82 | 南农一点莓Nannong Yidianmei |
19 | 南农香金针Nannong Xiangjinzhen | 51 | Q12-38-7 | 83 | Qx-013 |
20 | Q12-17-3 | 52 | Q11-24-56 | 84 | Qx-038 |
21 | Q06-8 | 53 | Q01-7 | 85 | 南农小草莓Nannong Xiaocaomei |
22 | Q16-8 | 54 | 南农香橙点Nannong Xiangchengdian | 86 | 南农紫星Nannong Zixing |
23 | Q11-11-5 | 55 | Q12-6-3 | 87 | Q04-56 |
24 | Q11-11-4 | 56 | Q01-51 | 88 | 球早Qiuzao |
25 | Q03-9 | 57 | 绿安娜Lüanna | 89 | 南农粉丽Nannong Fenli |
26 | 南农白蝴蝶Nannong Baihudie | 58 | QD3-170 | 90 | Qx-161 |
27 | 南农庐月Nannong Luyue | 59 | Q03-7 | 91 | 南农旋绿Nannong Xuanlü |
28 | Q06-42 | 60 | 南农瑗绿Nannong Ailü | 92 | 南农极点Nannong Jidian |
29 | Q01-70 | 61 | Qx-036 | 93 | Q01-38 |
30 | Q12-9-1 | 62 | Q06-3 | 94 | Q01-58 |
31 | 南农粉樱Nannong Fenying | 63 | 南农彩霞Nannong Caixia | 95 | 南农茶语Nannong Chayu |
32 | 南农佳丽Nannong Jiali | 64 | Q12-5-4 |
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