油菜素内酯对盐碱胁迫下平邑甜茶幼苗生长的影响及调控机理研究

doi:10.16420/j.issn.0513-353x.2021-0499

园艺学报 ›› 2022, Vol. 49 ›› Issue (7): 1401-1414.doi: 10.16420/j.issn.0513-353x.2021-0499

• 研究论文 • 下一篇

油菜素内酯对盐碱胁迫下平邑甜茶幼苗生长的影响及调控机理研究

郑晓东¹, 袭祥利¹, 李玉琪¹, 孙志娟², 马长青¹, 韩明三³, 李少旋³, 田义轲¹, 王彩虹¹^,^**()

¹青岛农业大学园艺学院,青岛农业大学果树遗传改良与生物技术实验室,山东青岛 266109
²青岛农业大学生命科学学院,山东青岛 266109
³青岛市农业科学研究院,山东青岛 266000

收稿日期:2022-01-17 修回日期:2022-03-29 出版日期:2022-07-25 发布日期:2022-07-29
通讯作者: 王彩虹 E-mail:chw6068@126.com
基金资助:
国家自然科学基金项目(31901992);山东省现代农业产业技术体系项目(SDAIT-06-06);山东省现代农业产业技术体系项目(SDAIT-06-09);2019年山东省高等学校青创人才引育计划项目(果树分子生物学研究创新团队)

Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress

ZHENG Xiaodong¹, XI Xiangli¹, LI Yuqi¹, SUN Zhijuan², MA Changqing¹, HAN Mingsan³, LI Shaoxuan³, TIAN Yike¹, WANG Caihong¹^,^**()

¹Laboratory of Fruit Tree Genetic Improvement and Biotechnology,College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China
²College of Life Science,Qingdao Agricultural University,Qingdao, Shandong 266109,China
³Qingdao Academy of Agricultural Sciences,Qingdao,Shandong 266000,China

Received:2022-01-17 Revised:2022-03-29 Online:2022-07-25 Published:2022-07-29
Contact: WANG Caihong E-mail:chw6068@126.com

摘要/Abstract

摘要：

为探究油菜素内酯对盐碱胁迫下苹果植株生长的影响,以具有无融合生殖特性的苹果砧木平邑甜茶（Malus hupehensis）为试材,研究外源2,4-表油菜素内酯（EBL,油菜素内酯类似物）对盐碱胁迫下植株体内离子稳态、渗透调节、抗氧化系统以及pH平衡的影响。结果表明:外源EBL具有显著增强平邑甜茶植株对盐碱胁迫抗性的效应。其主要作用机制为:通过调节钠钾离子转运基因的表达,提高细胞中的钾钠比例以维持离子稳态;通过积累脯氨酸和可溶性糖调节渗透胁迫,并通过提高POD和CAT活性减少盐碱胁迫下的氧化损伤。另外,外源EBL可通过增强根系分泌有机酸和提高MhAHA2、MhAHA8基因的表达,降低根系pH。外源EBL还可以通过与生长素、赤霉素和二氢玉米素协同作用共同应答盐碱胁迫。

关键词: 苹果, 平邑甜茶, 砧木, 盐碱胁迫, 油菜素内酯, 离子稳态, 氧化损伤

Abstract:

In order to explore the effect of brassinosteroids on the growth of apple plants under saline-alkali stress,apple rootstock Malus hupehensis with apomixis characteristics were used as experimental materials. The study investigated the effects of exogenous EBL（brassinosteroids analogs）on ion homeostasis,osmotic regulation,antioxidant system and pH balance of M. hupehensis under saline-alkali stress. The results showed that application of 0.2 mg · L^-1 exogenous EBL could significantly enhance the tolerance of M. hupehensis to saline-alkali stress. The main mechanisms were as follows:exogenous EBL could regulate the expression of Na⁺/K⁺transporter genes and enhance the ratio of potassium to sodium to maintain ion homeostasis. Moreover,exogenous EBL could accumulate proline and soluble sugar to modulate osmotic stress,and enhance the activities of POD and CAT to alleviate oxidative damage under saline-alkali stress. Furthermore,exogenous EBL could decrease root pH by improving root secretion of organic acids and enhancing the expression of MhAHA2 and MhAHA8 genes. Exogenous EBL could also cooperate with auxin,gibberellin and dihydrozeatin responding to saline-alkali stress.

Key words: apple, Malus hupehensis, rootstock, saline-alkali stress, brassinosteroids, ion homeostasis, oxidative damage

中图分类号:

S661.1

郑晓东, 袭祥利, 李玉琪, 孙志娟, 马长青, 韩明三, 李少旋, 田义轲, 王彩虹. 油菜素内酯对盐碱胁迫下平邑甜茶幼苗生长的影响及调控机理研究[J]. 园艺学报, 2022, 49(7): 1401-1414.

ZHENG Xiaodong, XI Xiangli, LI Yuqi, SUN Zhijuan, MA Changqing, HAN Mingsan, LI Shaoxuan, TIAN Yike, WANG Caihong. Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress[J]. Acta Horticulturae Sinica, 2022, 49(7): 1401-1414.

图/表 12

表1 qRT-PCR所用的引物序列

Table 1 Primer sequences for qRT-PCR

基因名称 Gene name	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
MhCAX5	ACCAGTCTCACTCTTTGTGGCG	GAATATAATTGGTGGGAGGAGATAT
MhCHX15	CCTCTTGGTACAGCATTGATAAAAA	GTTTGAACTTAATTTTGCAGCACA
MhSOS1	TACATCATTTCTGGTATATCTTGTG	CAAGATGAAAATTAAGGTATTAGCA
MhALT1	GTTGTTTTTTTGCTCTGACGACT	GTTGAACCACAAAACCCTGCT
MhSKOR	CATCCTGACAACTGGTGGTATCG	AAGTACCTCAGAGCAATCCGTTT
MhNHX4	ACGAAACTCCTTTACTATACAGCCT	TGATACCACAGATAAGTGAGCATAG
MhAHA2	AAAGTGTGAAGAAAGAGAAAATGC	AGTTCATTTGCTTGACATTCTTT
MhAHA8	GGTCAAAAGTGTGTTGGTAAGCA	AAACCAAAATCCTCCAACAGC
MhMAPKKKa	GCTACATGGGACATGACATTACTAG	CCAAGATGTTCCTGCCTTTTAT
MhLECPK91	TTCACCACTTATGCCAATGATAC	GGAACTGGAGATTCTTGCTTTTT
MhERF109	ATGCCCTTCCATGCGAATAG	CTAGTTCATGGACAACCATGCC
MhNAC56	ATGGAGTGCACCGACTCGTC	CTATCCCAAATTGGACTCAGAATAC
MhActin	CTTCAATGTGCCTGCCATGTAT	AATTTCCCGTTCAGCAGTAGTG

图1 盐碱胁迫和外源EBL处理0和15 d时平邑甜茶幼苗表型

Fig. 1 The phenotype of Malus hupehensis under saline-alkali stress and exogenous EBL treatment at 0 and 15 d

图2 盐碱胁迫和外源EBL处理15 d时平邑甜茶植株的萎蔫率、鲜质量和干质量不同小写字母表示0.05水平下差异显著。

Fig. 2 Effects of saline-alkali stress and exogenous EBL treatment on wilting rate,fresh weight and dry weight of Malus hupehensis seedlingsDifferent lowercase letters indicate significant difference at the 0.05 probability level.

图3 盐碱胁迫和外源EBL处理对平邑甜茶植株的叶绿素含量和光合速率的影响

Fig. 3 Effects of saline-alkali stress and exogenous EBL treatment on chlorophyll content and photosynthetic rate of Malus hupehensis

图4 盐碱胁迫和外源EBL处理对平邑甜茶植株体内离子含量的影响

Fig. 4 Effects of saline-alkali stress and exogenous EBL treatment on ion content of Malus hupehensis

图5 盐碱胁迫和外源EBL处理对平邑甜茶脯氨酸、可溶性糖、可溶性蛋白含量的影响

Fig. 5 Effects of saline-alkali stress and exogenous EBL treatment on the content of proline,soluble sugar,and soluble protein in Malus hupehensis

图6 盐碱胁迫和外源EBL处理对平邑甜茶MDA、超氧阴离子、H2O2含量和抗氧化酶活性的影响

Fig. 6 Effects of saline-alkali stress and exogenous EBL treatment on the content of MDA,superoxide anion,H2O2 and the activities of autioxidant enzyme in Malus hupehensis

图7 盐碱胁迫和外源EBL处理对平邑甜茶根系TTC染色

Fig. 7 Effects of saline-alkali stress and exogenous EBL treatment on root of Malus hupehensis by dyeing of TTC

图8 盐碱胁迫和外源EBL处理对平邑甜茶叶片苹果酸含量和柠檬酸含量的影响

Fig. 8 Effects of saline-alkali stress and exogenous EBL treatment on malic acid and citric acid content in Malus hupehensis leaves

图9 盐碱胁迫和外源EBL处理对平邑甜茶叶片中内源激素的影响

Fig. 9 Effects of saline-alkali stress and exogenous EBL treatment on the contents of endogenous hormones in Malus hupehensis leaves

图10 盐碱胁迫和外源EBL处理对平邑甜茶盐碱胁迫应答基因表达的影响

Fig. 10 Effects of saline-alkali stress and exogenous EBL treatment on the expression of saline-alkali stress response genes in Malus hupehensis

图11 外源EBL增强平邑甜茶植株耐盐碱性模式图

Fig. 11 Pattern diagram of exogenous EBL enhancing salt-alkali tolerance of Malus hupehensis

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油菜素内酯对盐碱胁迫下平邑甜茶幼苗生长的影响及调控机理研究

Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress

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油菜素内酯对盐碱胁迫下平邑甜茶幼苗生长的影响及调控机理研究

Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress

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