桃漆酶家族基因鉴定及PpLAC21功能分析

doi:10.16420/j.issn.0513-353x.2021-0767

园艺学报 ›› 2022, Vol. 49 ›› Issue (6): 1351-1362.doi: 10.16420/j.issn.0513-353x.2021-0767

桃漆酶家族基因鉴定及PpLAC21功能分析

邱可立¹^,², 王玉民³, 何金铃², 俞红¹^,⁴, 潘海发¹, 盛玉¹, 谢庆梅¹, 陈红莉¹, 周晖¹^,^**(), 张金云¹^,^**()

¹安徽省农业科学院园艺研究所,园艺作物种质创制及生理生态安徽省重点实验室,合肥 230031
²安徽农业大学生命科学学院,合肥 230036
³亳州学院生物与食品工程系,安徽亳州 236800
⁴安徽农业大学林学与园林学院,合肥 230036

收稿日期:2021-10-29 修回日期:2021-12-14 出版日期:2022-06-25 发布日期:2022-07-05
通讯作者: 周晖,张金云 E-mail:huichou1987@126.com;zjy600@aaas.org.cn
基金资助:
国家现代农业产业技术体系建设专项资金项目(CARS-30-Z-05);安徽省果树产业技术体系项目(AHNYCYTX-10);安徽省重点研究和开发计划项目(202104a06020023);安徽省重点研究和开发计划项目(201904a06020047)

Identification of Peach Laccase Family Genes and Function Analysis of PpLAC21

QIU Keli¹^,², WANG Yumin³, HE Jinling², YU Hong¹^,⁴, PAN Haifa¹, SHENG Yu¹, XIE Qingmei¹, CHEN Hongli¹, ZHOU Hui¹^,^**(), ZHANG Jinyun¹^,^**()

¹Key Laboratory of Anhui Genetic Improvement and Ecophysiology of Horticultural Crop,Instisute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei 230031,China
²School of Life Science,Anhui Agricultural University,Hefei 230036,China
³School of Biological and Food Engineering,Bozhou University,Bozhou,Anhui 236800,China
⁴School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036,China

Received:2021-10-29 Revised:2021-12-14 Online:2022-06-25 Published:2022-07-05
Contact: ZHOU Hui,ZHANG Jinyun E-mail:huichou1987@126.com;zjy600@aaas.org.cn

摘要/Abstract

摘要：

利用生物信息技术鉴定了桃中漆酶（LAC）家族成员,分析其进化关系分析、基因结构、启动子区顺式作用元件以及表达模式。结果表明,在桃基因组中共鉴定出48个漆酶基因,通过在桃子叶愈伤组织中的表达模式分析,发现1个表达量很高的关键成员PpLAC21,在该基因沉默的桃愈伤组织中,木质素的含量下降,推测该基因可能参与桃愈伤组织的木质素合成。

关键词: 桃, 漆酶, 愈伤组织, 病毒介导的基因沉默（VIGS）

Abstract:

The members of laccase（LAC）family in peach[Prunus persica L.（Batsch）] were identified by bioinformatics technology,and their evolutionary relationship,gene structure,cis-elements in promoter region and expression pattern were analyzed. The results showed that a total of 48 laccase genes were identified in peach genome. Through the analysis of expression pattern in peach cotyledon callus,it was found that a key member with high expression,PpLAC21,decreased the content of lignin in peach callus silenced by this gene. It is speculated that this gene may be involved in lignin synthesis in peach callus.

Key words: peach, laccase, callus, virus-induced gene silencing

中图分类号:

S662.1

邱可立, 王玉民, 何金铃, 俞红, 潘海发, 盛玉, 谢庆梅, 陈红莉, 周晖, 张金云. 桃漆酶家族基因鉴定及PpLAC21功能分析[J]. 园艺学报, 2022, 49(6): 1351-1362.

QIU Keli, WANG Yumin, HE Jinling, YU Hong, PAN Haifa, SHENG Yu, XIE Qingmei, CHEN Hongli, ZHOU Hui, ZHANG Jinyun. Identification of Peach Laccase Family Genes and Function Analysis of PpLAC21[J]. Acta Horticulturae Sinica, 2022, 49(6): 1351-1362.

图/表 10

参考文献 30

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基因 Gene	引物序列（5′-3′） Primer sequence
TEF2	F：GGTGTGACGATGAAGAGTGATG：R：TGAAGGAGAGGGAAGGTGAAAG
PpLAC7	F：AGGGCAGATAACCCAGGCA：R：AGGTGGTCGCAGAGTCTCATT
PpLAC15	F：CCTAAAACTATCGACAAGCGAGTT：R：TGACAAGGAGGGACGGACAA
PpLAC19	F：GGGCTGATAATCCAGGAGTTTG：R：CCTTTGCCATTGTCCACCA
PpLAC20	F：GGGTGGCTATCCGATTTCTG：R：AGCTTCCCATCCTGGACTACC
PpLAC21	F：CGACCCTGTTGAAAGAAATACC：R：CCATACACCGGGATTATCTGCT
PpLAC22	F：GCCCAAATGTCTCTGATGCTTA：R：CTCATCATTCAGTGCTGCGTT
PpLAC24	F：CACCAAACTCCTCGGATGC：R：TCCCTTGCTCAGCCTCCAT
PpLAC27	F：GCTGGCGGTTGGATTGC：R：GATCCACGCCATCCTTAGC
PpLAC28	F：CCACCCACTTCACCTCCAT：R：CGCCAACGGTGTTCCTCT
PpLAC30	F：GCTATCCGATTTCAAGCAGATAA：R：TTGATTCATTCGGTCCTTTCC
PpLAC33	F：TGGCACCAAACACCTCTGAC：R：CCCTTGCTCAACCTCCATAACA
PpLAC43	F：GCACTACTCACTGCCAACCTT：R：TGAAGTTCCCAGTTCCATCCA
PpLAC44	F：TCTCATCCCTTCCACCTTCAT：R：ATACTTTGCCGGGTCCTGTT
PpLAC45	F：GCTGCAAGACACCAATCTTCTC：R：GGCAACCCAACCACCTGTA

基因 Gene	引物序列（5′-3′） Primer sequence
TEF2	F：GGTGTGACGATGAAGAGTGATG：R：TGAAGGAGAGGGAAGGTGAAAG
PpLAC7	F：AGGGCAGATAACCCAGGCA：R：AGGTGGTCGCAGAGTCTCATT
PpLAC15	F：CCTAAAACTATCGACAAGCGAGTT：R：TGACAAGGAGGGACGGACAA
PpLAC19	F：GGGCTGATAATCCAGGAGTTTG：R：CCTTTGCCATTGTCCACCA
PpLAC20	F：GGGTGGCTATCCGATTTCTG：R：AGCTTCCCATCCTGGACTACC
PpLAC21	F：CGACCCTGTTGAAAGAAATACC：R：CCATACACCGGGATTATCTGCT
PpLAC22	F：GCCCAAATGTCTCTGATGCTTA：R：CTCATCATTCAGTGCTGCGTT
PpLAC24	F：CACCAAACTCCTCGGATGC：R：TCCCTTGCTCAGCCTCCAT
PpLAC27	F：GCTGGCGGTTGGATTGC：R：GATCCACGCCATCCTTAGC
PpLAC28	F：CCACCCACTTCACCTCCAT：R：CGCCAACGGTGTTCCTCT
PpLAC30	F：GCTATCCGATTTCAAGCAGATAA：R：TTGATTCATTCGGTCCTTTCC
PpLAC33	F：TGGCACCAAACACCTCTGAC：R：CCCTTGCTCAACCTCCATAACA
PpLAC43	F：GCACTACTCACTGCCAACCTT：R：TGAAGTTCCCAGTTCCATCCA
PpLAC44	F：TCTCATCCCTTCCACCTTCAT：R：ATACTTTGCCGGGTCCTGTT
PpLAC45	F：GCTGCAAGACACCAATCTTCTC：R：GGCAACCCAACCACCTGTA

基因 Gene	ID	蛋白质长度/aa Protein length	等电点 pI	分子量/D Relative molecular mass	亚细胞定位 Subcellular localization
PpLAC1	Prupe.1G323200	578	9.18	65 744.34	过氧化物酶体 Peroxisome
PpLAC2	Prupe.1G357100	544	9.18	60 477.29	细胞外基质 Extracellular matrix
PpLAC3	Prupe.1G559300	568	8.98	64 123.39	溶酶体 Lysosome
PpLAC4	Prupe.2G191900	574	8.38	63 604.68	细胞外基质 Extracellular matrix
PpLAC5	Prupe.2G205500	584	5.40	64 495.92	溶酶体 Lysosome
PpLAC6	Prupe.2G205600	609	6.74	67 760.86	溶酶体 Lysosome
PpLAC7	Prupe.2G245800	558	9.02	61 240.19	细胞外基质 Extracellular matrix
PpLAC8	Prupe.2G257400	583	8.53	64 070.85	细胞外基质 Extracellular matrix
PpLAC9	Prupe.2G278000	592	6.54	66 358.74	细胞外基质 Extracellular matrix
PpLAC10	Prupe.2G325200	567	6.82	62 943.79	溶酶体 Lysosome
PpLAC11	Prupe.3G216000	542	8.39	60 174.81	细胞外基质 Extracellular matrix
PpLAC12	Prupe.4G018800	543	9.72	61 649.96	细胞外基质 Extracellular matrix
PpLAC13	Prupe.4G131900	597	7.99	67 085.54	细胞外基质 Extracellular matrix
PpLAC14	Prupe.5G075700	591	8.40	65 813.66	质膜 Plasma membrane
PpLAC15	Prupe.5G231100	582	7.06	65 497.57	溶酶体 Lysosome
PpLAC16	Prupe.6G000400	552	9.01	61 928.85	细胞外基质 Extracellular matrix
PpLAC17	Prupe.6G000500	554	8.84	62 137.61	细胞外基质 Extracellular matrix
PpLAC18	Prupe.6G048400	1 559	6.47	175 147.62	质膜 Plasma membrane
PpLAC19	Prupe.6G061300	558	9.43	61 628.12	细胞外基质 Extracellular matrix
PpLAC20	Prupe.6G072100	587	9.77	64 558.17	细胞外基质 Extracellular matrix
PpLAC21	Prupe.6G089100	583	9.33	64 487.37	溶酶体 Lysosome
PpLAC22	Prupe.6G089300	581	9.30	64 283.15	细胞外基质 Extracellular matrix
PpLAC23	Prupe.6G155100	577	6.81	64 268.78	细胞外基质 Extracellular matrix
PpLAC24	Prupe.6G177700	564	8.41	62 267.07	溶酶体 Lysosome
PpLAC25	Prupe.6G179500	580	7.09	65 306.02	细胞外基质 Extracellular matrix
基因 Gene	ID	蛋白质长度/aa Protein length	等电点 pI	分子量/D Relative molecular mass	亚细胞定位 Subcellular localization
PpLAC26	Prupe.6G242000	567	6.64	63 053.79	溶酶体 Lysosome
PpLAC27	Prupe.6G257700	585	9.02	64 453.73	质膜 Plasma membrane
PpLAC28	Prupe.6G258000	586	9.85	64 986.03	细胞外基质 Extracellular matrix
PpLAC29	Prupe.6G267700	563	5.95	61 867.26	细胞外基质 Extracellular matrix
PpLAC30	Prupe.6G271500	559	9.31	60 774.70	细胞外基质 Extracellular matrix
PpLAC31	Prupe.7G140500	539	9.29	60 306.38	细胞外基质 Extracellular matrix
PpLAC32	Prupe.7G140600	537	9.46	59 617.38	溶酶体 Lysosome
PpLAC33	Prupe.7G156500	587	8.65	65 072.51	细胞外基质 Extracellular matrix
PpLAC34	Prupe.8G046800	568	6.76	64 097.74	细胞外基质 Extracellular matrix
PpLAC35	Prupe.8G046900	578	9.08	65 169.63	细胞外基质 Extracellular matrix
PpLAC36	Prupe.8G047000	584	4.95	65 244.50	细胞外基质 Extracellular matrix
PpLAC37	Prupe.8G047100	570	5.52	62 952.86	质膜 Plasma membrane
PpLAC38	Prupe.8G047200	569	5.29	62 540.13	质膜 Plasma membrane
PpLAC39	Prupe.8G047300	569	5.11	62 344.97	质膜 Plasma membrane
PpLAC40	Prupe.8G047500	558	5.52	61 255.84	质膜 Plasma membrane
PpLAC41	Prupe.8G047900	564	5.58	62 057.08	质膜 Plasma membrane
PpLAC42	Prupe.8G048100	564	5.93	62 274.45	细胞外基质 Extracellular matrix
PpLAC43	Prupe.8G094800	367	9.69	40 256.36	质膜 Plasma membrane
PpLAC44	Prupe.8G095000	514	8.63	57 389.05	溶酶体 Lysosome
PpLAC45	Prupe.8G095400	563	8.78	62 198.39	溶酶体 Lysosome
PpLAC46	Prupe.8G097000	563	8.78	62 158.28	溶酶体 Lysosome
PpLAC47	Prupe.8G172900	536	9.26	59 889.78	细胞外基质 Extracellular matrix
PpLAC48	Prupe.8G189700	588	5.94	65 150.69	溶酶体 Lysosome

基因 Gene	ID	蛋白质长度/aa Protein length	等电点 pI	分子量/D Relative molecular mass	亚细胞定位 Subcellular localization
PpLAC1	Prupe.1G323200	578	9.18	65 744.34	过氧化物酶体 Peroxisome
PpLAC2	Prupe.1G357100	544	9.18	60 477.29	细胞外基质 Extracellular matrix
PpLAC3	Prupe.1G559300	568	8.98	64 123.39	溶酶体 Lysosome
PpLAC4	Prupe.2G191900	574	8.38	63 604.68	细胞外基质 Extracellular matrix
PpLAC5	Prupe.2G205500	584	5.40	64 495.92	溶酶体 Lysosome
PpLAC6	Prupe.2G205600	609	6.74	67 760.86	溶酶体 Lysosome
PpLAC7	Prupe.2G245800	558	9.02	61 240.19	细胞外基质 Extracellular matrix
PpLAC8	Prupe.2G257400	583	8.53	64 070.85	细胞外基质 Extracellular matrix
PpLAC9	Prupe.2G278000	592	6.54	66 358.74	细胞外基质 Extracellular matrix
PpLAC10	Prupe.2G325200	567	6.82	62 943.79	溶酶体 Lysosome
PpLAC11	Prupe.3G216000	542	8.39	60 174.81	细胞外基质 Extracellular matrix
PpLAC12	Prupe.4G018800	543	9.72	61 649.96	细胞外基质 Extracellular matrix
PpLAC13	Prupe.4G131900	597	7.99	67 085.54	细胞外基质 Extracellular matrix
PpLAC14	Prupe.5G075700	591	8.40	65 813.66	质膜 Plasma membrane
PpLAC15	Prupe.5G231100	582	7.06	65 497.57	溶酶体 Lysosome
PpLAC16	Prupe.6G000400	552	9.01	61 928.85	细胞外基质 Extracellular matrix
PpLAC17	Prupe.6G000500	554	8.84	62 137.61	细胞外基质 Extracellular matrix
PpLAC18	Prupe.6G048400	1 559	6.47	175 147.62	质膜 Plasma membrane
PpLAC19	Prupe.6G061300	558	9.43	61 628.12	细胞外基质 Extracellular matrix
PpLAC20	Prupe.6G072100	587	9.77	64 558.17	细胞外基质 Extracellular matrix
PpLAC21	Prupe.6G089100	583	9.33	64 487.37	溶酶体 Lysosome
PpLAC22	Prupe.6G089300	581	9.30	64 283.15	细胞外基质 Extracellular matrix
PpLAC23	Prupe.6G155100	577	6.81	64 268.78	细胞外基质 Extracellular matrix
PpLAC24	Prupe.6G177700	564	8.41	62 267.07	溶酶体 Lysosome
PpLAC25	Prupe.6G179500	580	7.09	65 306.02	细胞外基质 Extracellular matrix
基因 Gene	ID	蛋白质长度/aa Protein length	等电点 pI	分子量/D Relative molecular mass	亚细胞定位 Subcellular localization
PpLAC26	Prupe.6G242000	567	6.64	63 053.79	溶酶体 Lysosome
PpLAC27	Prupe.6G257700	585	9.02	64 453.73	质膜 Plasma membrane
PpLAC28	Prupe.6G258000	586	9.85	64 986.03	细胞外基质 Extracellular matrix
PpLAC29	Prupe.6G267700	563	5.95	61 867.26	细胞外基质 Extracellular matrix
PpLAC30	Prupe.6G271500	559	9.31	60 774.70	细胞外基质 Extracellular matrix
PpLAC31	Prupe.7G140500	539	9.29	60 306.38	细胞外基质 Extracellular matrix
PpLAC32	Prupe.7G140600	537	9.46	59 617.38	溶酶体 Lysosome
PpLAC33	Prupe.7G156500	587	8.65	65 072.51	细胞外基质 Extracellular matrix
PpLAC34	Prupe.8G046800	568	6.76	64 097.74	细胞外基质 Extracellular matrix
PpLAC35	Prupe.8G046900	578	9.08	65 169.63	细胞外基质 Extracellular matrix
PpLAC36	Prupe.8G047000	584	4.95	65 244.50	细胞外基质 Extracellular matrix
PpLAC37	Prupe.8G047100	570	5.52	62 952.86	质膜 Plasma membrane
PpLAC38	Prupe.8G047200	569	5.29	62 540.13	质膜 Plasma membrane
PpLAC39	Prupe.8G047300	569	5.11	62 344.97	质膜 Plasma membrane
PpLAC40	Prupe.8G047500	558	5.52	61 255.84	质膜 Plasma membrane
PpLAC41	Prupe.8G047900	564	5.58	62 057.08	质膜 Plasma membrane
PpLAC42	Prupe.8G048100	564	5.93	62 274.45	细胞外基质 Extracellular matrix
PpLAC43	Prupe.8G094800	367	9.69	40 256.36	质膜 Plasma membrane
PpLAC44	Prupe.8G095000	514	8.63	57 389.05	溶酶体 Lysosome
PpLAC45	Prupe.8G095400	563	8.78	62 198.39	溶酶体 Lysosome
PpLAC46	Prupe.8G097000	563	8.78	62 158.28	溶酶体 Lysosome
PpLAC47	Prupe.8G172900	536	9.26	59 889.78	细胞外基质 Extracellular matrix
PpLAC48	Prupe.8G189700	588	5.94	65 150.69	溶酶体 Lysosome

桃漆酶家族基因鉴定及PpLAC21功能分析

Identification of Peach Laccase Family Genes and Function Analysis of PpLAC21

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桃漆酶家族基因鉴定及PpLAC21功能分析

Identification of Peach Laccase Family Genes and Function Analysis of PpLAC21

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摘要/Abstract

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