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园艺学报 ›› 2006, Vol. 33 ›› Issue (5): 953-956.

• 研究论文 • 上一篇    下一篇

矮化蟠桃的育种实践与连锁遗传分析

牛良;王志强;刘淑娥;宋银花;宗学普   

  1. (中国农业科学院郑州果树研究所, 河南郑州450009)
  • 收稿日期:2005-11-13 修回日期:2006-03-13 出版日期:2006-10-25 发布日期:2006-10-25

Linkage Analysis between Genes for Dwarf Tree and Round Fruit Shape inPeach ( Prunus persica L. )

Niu Liang; Wang Zhiqiang; Liu Shupe; Song Yinhua;Zong Xuepu   

  1. ( Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan 450009, China)
  • Received:2005-11-13 Revised:2006-03-13 Online:2006-10-25 Published:2006-10-25

摘要: 利用乔化异质型蟠桃(DwdwSs) 与矮化型圆桃( dwdwss) 或乔化异质型圆桃(Dwdwss) 进行有性杂交, 培育杂种实生苗, 对子代相关性状的分离情况进行了分析。结果显示, 乔化对矮化为一对等位基因, 且乔化对矮化为完全显性, 蟠桃与圆桃为一对等位基因, 蟠桃对圆桃为完全显性。在乔化异质型蟠桃(DwdwSs) 与矮化型圆桃( dwdwss) 的正反交后代中, 没有出现矮化型蟠桃和乔化型圆桃, 乔化蟠桃与矮化圆桃的比例为1∶1; 在乔化异质型蟠桃(DwdwSs) 与乔化异质型圆桃(Dwdwss) 的杂交后代中, 也同样没有出现矮化型蟠桃, 乔化蟠桃、乔化圆桃与矮化圆桃的分离比例为2∶1∶1, 据此得出以下推论, 果实扁平性状与树体乔化性状完全连锁, 树体矮化性状与果实非扁平性状完全连锁, 即矮化突变与扁平果形突变分别发生在不同的同源染色体上, 两者之间没有发生过交换, 不产生dwS配子。

关键词: 蟠桃, 矮化, 育种, 连锁, 遗传

Abstract: Using 83 seedlings of 4 crosses between genotypes of DwdwSs and dwdwss, 18 seedlings of 1 cross between genotypes of Dwdwss and DwdwSs, we analyzed the segregation of the two peach traits based on the progenies. The results showed dwarf growth habit and flat fruit shape are qualitative inheritance. Dwarf growth habit is a recessive characterwhile flat fruit shape is dominant. In p rogenies of the cross combinations of DwdwSs ×dwdwss or dwdwss ×DwdwSs, there were no dwarf flat peach and non-dwarf round peach observed. The segregation ratio of Dw_Ss and dwdwss was 1∶1. The two pairs of allelic gene were isolated with Mendelian ratio independently. We also failed getting dwarf flat peach in the cross combination of DwdwSs ×
Dwdwss, in progenies of which the segregation ratio of Dw _ SsDw _ ssdwdwss was 2∶1∶1. Based on above results, we supposed that dwarf tree ( dw ) was completely linked with round peach fruit (s) , while flat peach fruit (S) was linked with non-dwarf tree (Dw). The dwarf gene and the gene controlled the flat fruit shape was located in different homologous chromosomes and there was no crossover happened. As a result, there was no dwS gamete produced.

Key words: Flat peach, Dwarf, Breeding, Complete linkage, Inherent