侵染凤仙花的菜豆金色花叶病毒的鉴定及基因组结构分析

doi:10.16420/j.issn.0513-353x.2021-0221

摘要/Abstract

摘要：

在对云南省西双版纳州进行病毒病害调查时发现1株疑似菜豆金色花叶病毒属病毒侵染的凤仙花植株,其症状表现为叶片皱缩和花叶。利用菜豆金色花叶病毒属病毒简并引物进行PCR扩增、克隆和分析,获得YN6198-28和YN6198-31两条菜豆金色花叶病毒属病毒的DNA-A全长序列,长度均为2 763 bp,编码6个开放阅读框,其中YN6198-28核苷酸序列与越南丁香黄脉病毒（Ludwigia yellow vein Vietnam virus,LuYVVNV）相似性为94.90%,表明该分离物是LuYVVNV1个分离物。重组分析发现,YN6198-28是种间重组病毒,其主要亲本为丁香黄脉病毒（Ludwigia yellow vein virus,LuYVV）,次要亲本为中国胜红蓟黄脉病毒（Ageratum yellow vein China virus,AYVCNV）。

关键词: 凤仙花, 菜豆金色花叶病毒属, 重组病毒

Abstract:

An investigation about diseases in Xishuangbanna Autonomous Prefecture,Yunnan Province identified an Impatiens balsamina plant with leaf shrinking and mosaic symptoms typical of begomovirus infections. To identify the possible virus associated with the disease,the sample was collected and detected by PCR with begomovirus degenerate primers. The amplicons were cloned and sequenced,and two sequences（YN6198-28 and YN6198-31）of 2 763 bp corresponding to begomovirus DNA-A genome with six open reading frames were identified from this sample. The YN6198-28 DNA-A sequence shares the highest identity（94.90%）with Ludwigia yellow vein Vietnam virus（LuYVVNV）,which confirmed that the virus was an isolate of LuYVVNV. Recombination analysis revealed that the YN6198-28 isolate is a recombinant begomovirus derived from Ludwigia yellow vein virus and Ageratum yellow vein China virus.

Key words: Impatiens balsamina, Begomoviruses, recombination virus

中图分类号:

S681.1

钟静, 赵丽玲, 李婷婷, 张水英, 陈越, 丁铭. 侵染凤仙花的菜豆金色花叶病毒的鉴定及基因组结构分析[J]. 园艺学报, 2022, 49(5): 1136-1144.

ZHONG Jing, ZHAO Liling, LI Tingting, ZHANG Shuiying, CHEN Yue, DING Ming. Molecular Identification and Sequence Analysis of Begomoviruses Infecting Impatiens balsamina[J]. Acta Horticulturae Sinica, 2022, 49(5): 1136-1144.

图/表 5

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病毒 Viruses	分离物 Isolate	登录号 Accession No.	Total Nt	IR	V1		V2		C1		C2		C3		C4
病毒 Viruses	分离物 Isolate	登录号 Accession No.	Total Nt	IR	Nt	AA	Nt	AA	Nt	AA	Nt	AA	Nt	AA	Nt	AA
AlYVV	P-663	MH538342.1	73.33	65.82	80.16	92.96	73.40	46.77	73.66	72.85	76.29	60.66	73.58	67.91	72.50	46.87
AlYVV	YN598	FJ015062.1	73.62	67.03	80.00	92.46	75.00	50.00	72.55	73.40	76.54	60.66	75.55	70.89	70.10	42.70
AYVCNV	BS1	KU954377.1	79.22	75.88	73.74	80.40	84.81	69.84	83.74	87.56	80.39	71.85	80.89	76.86	88.65	76.04
AYVCNV	BS3	KU954379.1	78.51	74.50	74.41	80.40	78.01	55.55	83.83	87.01	79.65	71.11	79.50	75.37	89.00	77.08
AYVV	Thai3	MK238277.1	78.04	76.40	75.25	81.40	82.72	71.42	80.38	86.46	81.86	73.33	80.74	77.61	72.91	43.75
AYVV	YN2528-3	MN233600.1	79.36	78.71	73.07	80.90	83.24	66.66	83.47	88.12	80.63	72.59	80.64	75.37	83.84	63.54
CoLCV	Lucknow	GU440580.1	79.67	80.24	74.74	80.90	77.12	59.67	83.37	86.31	82.59	73.33	84.44	82.83	84.19	72.91
LuYVV		DQ641708.1	75.97	82.53	96.83	98.99	99.47	92.06	84.29	80.66	99.50	100.00	97.03	97.01	54.60	47.05
LuYVV	G37	AJ965539.2	75.88	87.30	97.50	96.55	98.95	92.06	81.34	77.17	98.03	98.51	96.79	95.52	73.15	47.05
LuYVVNV		DQ641699.1	94.90	86.64	97.16	99.49	99.47	93.65	94.18	94.19	98.77	97.77	95.80	97.01	92.43	86.45
LuYVVNV	YN6154	MN210349.1	94.61	80.00	97.33	100.00	98.42	92.06	94.03	95.85	99.26	97.77	97.53	98.50	96..21	93.75
SaLCV	AFSP5e	NC_038455.1	89.26	88.03	95.83	96.98	82.72	66.66	85.85	87.29	96.56	97.03	98.02	97.01	96..21	55.29
SiYVVNV		NC_009547.1	79.94	75.19	75.16	81.40	85.34	66.66	82.82	85.08	83.08	77.77	86.66	81.34	84.87	69.79
TbLCPatV	INBD	KM383746.1	80.15	75.49	73.95	80.90	77.48	58.73	85.04	89.44	85.29	77.77	86.17	83.58	84.53	69.79
ToLCMaV	MT1-5	NC_004648.1	80.02	75.30	74.74	80.40	80.20	61.90	84.94	88.12	82.35	76.29	85.18	77.61	86.94	71.87
ToLCPatV	Ballia	MK122994.1	79.42	76.19	74.74	80.40	77.48	58.73	83.62	86.11	82.10	74.07	84.19	80.59	84.53	72.91
TYLCYnV	YN2013-10	NC_021341.1	78.34	77.82	74.50	81.90	79.58	60.31	83.51	87.81	79.50	70.14	78.10	71.64	83.84	66.66
TYLCYnV	YN4265-4	MN233598.1	78.11	76.21	74.50	81.90	78.23	50.79	83.05	86.14	80.24	71.64	78.90	73.13	83.84	64.58

病毒 Viruses	分离物 Isolate	登录号 Accession No.	Total Nt	IR	V1		V2		C1		C2		C3		C4
病毒 Viruses	分离物 Isolate	登录号 Accession No.	Total Nt	IR	Nt	AA	Nt	AA	Nt	AA	Nt	AA	Nt	AA	Nt	AA
AlYVV	P-663	MH538342.1	73.33	65.82	80.16	92.96	73.40	46.77	73.66	72.85	76.29	60.66	73.58	67.91	72.50	46.87
AlYVV	YN598	FJ015062.1	73.62	67.03	80.00	92.46	75.00	50.00	72.55	73.40	76.54	60.66	75.55	70.89	70.10	42.70
AYVCNV	BS1	KU954377.1	79.22	75.88	73.74	80.40	84.81	69.84	83.74	87.56	80.39	71.85	80.89	76.86	88.65	76.04
AYVCNV	BS3	KU954379.1	78.51	74.50	74.41	80.40	78.01	55.55	83.83	87.01	79.65	71.11	79.50	75.37	89.00	77.08
AYVV	Thai3	MK238277.1	78.04	76.40	75.25	81.40	82.72	71.42	80.38	86.46	81.86	73.33	80.74	77.61	72.91	43.75
AYVV	YN2528-3	MN233600.1	79.36	78.71	73.07	80.90	83.24	66.66	83.47	88.12	80.63	72.59	80.64	75.37	83.84	63.54
CoLCV	Lucknow	GU440580.1	79.67	80.24	74.74	80.90	77.12	59.67	83.37	86.31	82.59	73.33	84.44	82.83	84.19	72.91
LuYVV		DQ641708.1	75.97	82.53	96.83	98.99	99.47	92.06	84.29	80.66	99.50	100.00	97.03	97.01	54.60	47.05
LuYVV	G37	AJ965539.2	75.88	87.30	97.50	96.55	98.95	92.06	81.34	77.17	98.03	98.51	96.79	95.52	73.15	47.05
LuYVVNV		DQ641699.1	94.90	86.64	97.16	99.49	99.47	93.65	94.18	94.19	98.77	97.77	95.80	97.01	92.43	86.45
LuYVVNV	YN6154	MN210349.1	94.61	80.00	97.33	100.00	98.42	92.06	94.03	95.85	99.26	97.77	97.53	98.50	96..21	93.75
SaLCV	AFSP5e	NC_038455.1	89.26	88.03	95.83	96.98	82.72	66.66	85.85	87.29	96.56	97.03	98.02	97.01	96..21	55.29
SiYVVNV		NC_009547.1	79.94	75.19	75.16	81.40	85.34	66.66	82.82	85.08	83.08	77.77	86.66	81.34	84.87	69.79
TbLCPatV	INBD	KM383746.1	80.15	75.49	73.95	80.90	77.48	58.73	85.04	89.44	85.29	77.77	86.17	83.58	84.53	69.79
ToLCMaV	MT1-5	NC_004648.1	80.02	75.30	74.74	80.40	80.20	61.90	84.94	88.12	82.35	76.29	85.18	77.61	86.94	71.87
ToLCPatV	Ballia	MK122994.1	79.42	76.19	74.74	80.40	77.48	58.73	83.62	86.11	82.10	74.07	84.19	80.59	84.53	72.91
TYLCYnV	YN2013-10	NC_021341.1	78.34	77.82	74.50	81.90	79.58	60.31	83.51	87.81	79.50	70.14	78.10	71.64	83.84	66.66
TYLCYnV	YN4265-4	MN233598.1	78.11	76.21	74.50	81.90	78.23	50.79	83.05	86.14	80.24	71.64	78.90	73.13	83.84	64.58

重组分离物 Recombinant isolate	主要亲本 Major parent	次要亲本 Mino parent	重组发生位置/nt Break point	重组检测 Detected of recombination events
重组分离物 Recombinant isolate	主要亲本 Major parent	次要亲本 Mino parent	重组发生位置/nt Break point	方法Method	P
LuYVVNV（MN210346）	LuYVV	AYVCNV-BS3	2 082 ~ 2 640	RDP	1.146 × 10^-38
LuYVVNV（MN210349）	LuYVV	AYVCNV-BS3	2 094 ~ 2 607	GENECONV	6.965 × 10^-18
LuYVVNV（NC_038451）	LuYVV	AYVCNV-BS3	2 094 ~ 2 576	BootScan	3.351 × 10^-27
LuYVVNV（DQ641699）	AYVCNV-BS3	LuYVV	2 094 ~ 2 578	MaxChi	5.266 × 10^-21
LuYVVNV（MN210345）	AYVCNV-BS3	LuYVV	2 094 ~ 2 607	Chimaera	1.086 × 10^-17
				SiScan	2.001 × 10^-16
				3Seq	5.476 × 10^-48

重组分离物 Recombinant isolate	主要亲本 Major parent	次要亲本 Mino parent	重组发生位置/nt Break point	重组检测 Detected of recombination events
重组分离物 Recombinant isolate	主要亲本 Major parent	次要亲本 Mino parent	重组发生位置/nt Break point	方法Method	P
LuYVVNV（MN210346）	LuYVV	AYVCNV-BS3	2 082 ~ 2 640	RDP	1.146 × 10^-38
LuYVVNV（MN210349）	LuYVV	AYVCNV-BS3	2 094 ~ 2 607	GENECONV	6.965 × 10^-18
LuYVVNV（NC_038451）	LuYVV	AYVCNV-BS3	2 094 ~ 2 576	BootScan	3.351 × 10^-27
LuYVVNV（DQ641699）	AYVCNV-BS3	LuYVV	2 094 ~ 2 578	MaxChi	5.266 × 10^-21
LuYVVNV（MN210345）	AYVCNV-BS3	LuYVV	2 094 ~ 2 607	Chimaera	1.086 × 10^-17
				SiScan	2.001 × 10^-16
				3Seq	5.476 × 10^-48