石榴HAK/KUP/KT家族基因鉴定及钾转运功能分析

doi:10.16420/j.issn.0513-353x.2021-0202

园艺学报 ›› 2022, Vol. 49 ›› Issue (4): 758-768.doi: 10.16420/j.issn.0513-353x.2021-0202

石榴HAK/KUP/KT家族基因鉴定及钾转运功能分析

赵建荣¹, 杨圆¹^,², 秦改花²^,^*(), 刘春燕², 于晴²^,³, 贾波涛², 苏颖²^,³, 曹榛², 黎积誉²^,^*()

1.安徽科技学院资源与环境学院安徽凤阳 233100
2.安徽省农业科学院园艺研究所,园艺作物种质创制与生理生态安徽省重点实验室,果树果实发育与品质生物学重点实验室,合肥 230001
3.南京农业大学园艺学院,南京 210095

收稿日期:2021-11-23 修回日期:2022-01-12 出版日期:2022-04-25 发布日期:2022-04-24
通讯作者: 秦改花,黎积誉 E-mail:qghahstu@163.com;lijiyugx@163.com
基金资助:
安徽省科技重大专项(201903b06020017);安徽省科技重大专项(18030701214);淮北市科技重大专项(HK2021014)

Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate

ZHAO Jianrong¹, YANG Yuan¹^,², QIN Gaihua²^,^*(), LIU Chunyan², YU Qing²^,³, JIA Botao², SU Ying²^,³, CAO Zhen², LI Jiyu²^,^*()

1. College of Resource and Environment,Anhui Science and Technology University,Fengyang,Anhui 233100,China
2. Key Laboratory of Horticultural Crop Genetic Improvement and Eco-physiology of Anhui Province,Key Laboratory of Fruit Quality and Developmental Biology,Institute of Horticulture Research,Anhui Academy of Agricultural Sciences,Hefei 230001,China
3. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China

Received:2021-11-23 Revised:2022-01-12 Online:2022-04-25 Published:2022-04-24
Contact: QIN Gaihua,LI Jiyu E-mail:qghahstu@163.com;lijiyugx@163.com

摘要/Abstract

摘要：

从石榴基因组中鉴定出18个编码689 ~ 837个氨基酸的HAK/KUP/KT家族基因;启动子区含有多个与逆境胁迫相关的调控元件,编码蛋白中均含有一段K⁺转运结构域（GVVYGDLGTSPLY）;系统进化分析表明,石榴、拟南芥、葡萄和梨的HAK/KUP/KT家族基因均可分为4个簇（Ⅰ ~ Ⅳ）。RNA-seq分析发现簇Ⅱ和簇Ⅲ的多数基因在石榴不同组织中均有表达,簇Ⅰ中PgrHAK5仅在根中特异性表达。qRT-PCR分析发现,PgrHAK5在石榴根中有特异性高表达,其表达受缺钾胁迫的诱导。亚细胞定位表明PgrHAK5定位于细胞质膜上。酵母功能互补试验表明,PgrHAK5具有转运K⁺的功能,推测定位于质膜上的PgrHAK5参与石榴根系K⁺的跨质膜转运。

关键词: 石榴, 钾转运蛋白, PgrHAK5, 功能分析

Abstract:

In order to fully understand the mechanism of K⁺ uptake,bioinformatics analysis and function study of related genes of HAK/KUP/KT family in pomegranate were carried out in this study. Eighteen of HAK/KUP/KT family genes each encoded 689-837 amino acids were identified from the pomegranate genome. The promoter region contains several regulatory elements related to stress,and the gene-coding proteins contain a K⁺ transport domain（GVVYGDLGTSPLY）. Phylogenetic analysis showed that the HAK/KUP/KT family genes of pomegranate,Arabidopsis thaliana,grape and pear could be divided into four clusters（Ⅰ-Ⅳ）. RNA-seq analysis showed that most genes of cluster Ⅱ and cluster Ⅲ were expressed in different tissues of pomegranate,while PgrHAK5 in clusterⅠwas only specifically expressed in roots. qRT-PCR analysis showed that PgrHAK5 was highly expressed in roots,and its expression was induced by potassium deficiency stress. Subcellular localization indicated that the protein encoded by PgrHAK5 was localized on the plasma membrane,and PgrHAK5 can complement the defect of the yeast K⁺ transport mutant CY162,which suggest that PgrHAK5 play an important role in K⁺ transport in pomegranate roots.

Key words: pomegranate, potassium transporters, PgrHAK5, functional

中图分类号:

S665.4

赵建荣, 杨圆, 秦改花, 刘春燕, 于晴, 贾波涛, 苏颖, 曹榛, 黎积誉. 石榴HAK/KUP/KT家族基因鉴定及钾转运功能分析[J]. 园艺学报, 2022, 49(4): 758-768.

ZHAO Jianrong, YANG Yuan, QIN Gaihua, LIU Chunyan, YU Qing, JIA Botao, SU Ying, CAO Zhen, LI Jiyu. Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate[J]. Acta Horticulturae Sinica, 2022, 49(4): 758-768.

图/表 6

参考文献 43

[1]	Ahn S J, Shin R, Schachtman D P. 1992. 2004. Expression of KT/KUP genes in Arabidopsis and the role of root hairs in K⁺uptake. Plant Physiology, 134 (3):1135-1145. doi: 10.1104/pp.103.034660 URL
[2]	Alexander G. 2007. Plant KT/KUP/HAK potassium transporter:single family-multiple functions. Annals of Botany,(6):1035-1041. pmid: 17495982
[3]	Anderson J A, Huprikar S S, Kochian L V, Lucas W J, Gaber R F. 1992. Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae. Proc Natl Acad Sci USA, 89 (9):3736-3740. doi: 10.1073/pnas.89.9.3736 URL
[4]	Bañuelos M A, Garciadeblas B, Cubero B. 2002. Inventory and functional characterization of the HAK potassium transporters of rice. Plant Physiology, 130 (2):784-795. pmid: 12376644
[5]	Chai W W, Wang W Y, Cui Y N, Wang S M. 2019. Research progress of function on KUP/HAK/KT family in plants. Plant Physiology Journal, 55 (12):1747-1761.
[6]	Chen G, Hu Q D, Luo L, Yang T Y, Zhang S. 2015. Rice potassium transporter OsHAK 1 is essential for maintaining potassium-mediated growth and functions in salt tolerance over low and high potassium concentration ranges. Plant,Cell & Environment, 38 (12):2747-2765.
[7]	Chen G, Liu C, Gao Z Y, Yu Z, Jiang H, Zhu J, Ren D, Yu L, Xu G, Qian Q. 2017. OsHAK1,a high-affinity potassium transporter,positively regulates responses to drought stress in rice. Frontiers in Plant Science, 8:1885. doi: 10.3389/fpls.2017.01885 URL
[8]	Chen X Y, Liu X D, Mao W W, Zhang X R, Chen S L, Zhan K H, Bi H H, Xu H X. 2018. Genome-wide identification and analysis of HAK/KUP/KT potassium transporters gene family in wheat(Triticum aestivum L.). International Journal of Molecular Sciences, 19:12. doi: 10.3390/ijms19010012 URL
[9]	Davies C, Shin R, Liu W, Thomas M R, Schachtman, 2006. Transporters expressed during grape berry(Vitis vinifera L.)development are associated with an increase in berry size and berry potassium accumulation. Journal of Experimental Botany, 57 (12):3209-3216. doi: 10.1093/jxb/erl091 URL
[10]	Elumalai R P, Nagpal P, Reed J W. 2002. A mutation in the Arabidopsis KT2/KUP 2 potassium transporter gene affects shoot cell expansion. The Plant Cell Online, 14 (1):119-131. doi: 10.1105/tpc.010322 URL
[11]	Epstein E, Rains D W, Elzam O E. 1963. Resolution of dual mechanisms of potassium absorption by barley roots. Proc Natl Acad USA, 499 (5):684-692.
[12]	Gierth M, Schroeder P M I. 2005. The potassium transporter AtHAK5 functions in K⁺ deprivation-induced high-affinity K⁺ uptake and AKT1 K⁺ channel con to K⁺ uptake kontributiinetics in Arabidopsis roots. Plant Physiology, 137 (3):1105-1114. doi: 10.1104/pp.104.057216 URL
[13]	Gomez-Porras J L, Riaño-Pachón Diego Mauricio, Benito Begoña, Haro Rosario, Sklodowski Kamil, Rodríguez-Navarro Alonso, Dreyer Ingo. 2012. Phylogenetic analysis of K⁺ transporters in bryophytes,lycophytes,and flowering plants indicates a specialization of vascular plants. Frontiers in Plant Science, 3:167. doi: 10.3389/fpls.2012.00167 pmid: 22876252
[14]	Gupta M, Qiu X, Wang L, Xie W, Zhang C, Xiong L, Lian X, Zhang Q. 2008. KT/HAK/KUP potassium transporters gene family and their whole-life cycle expression profile in rice(Oryza sativa). Molecular Genetics & Genomics, 280 (5):437-452.
[15]	Hasanuzzaman M, Bhuyan M H M B, Nahar K, Hossain M S, Fujita M. 2018. Potassium:a vital regulator of plant responses and tolerance to abiotic stresses. Agronomy, 8:31. doi: 10.3390/agronomy8030031 URL
[16]	Hu B, Jin J, Guo A Y, He Z, Ge G. 2014. GSDS 2.0:an upgraded gene feature visualization server. Bioinformatics, 31(8):1296. doi: 10.1093/bioinformatics/btu817 URL
[17]	Lacombe B. 2000. A shaker-like K⁺ channel with weak rectification is expressed in both source and sink phloem tissues of Arabidopsis. The Plant Call Online, 12 (6):837-851.
[18]	Li W, Xu G, Alli A, Abdel L. 2018. Plant HAK/KUP/KT K⁺ transporters:function and regulation. Seminars in Cell & Developmental Biology, 74:133-141.
[19]	Ling Qiuping, Zeng Qiaoying, Hu Fei, Wu Jiayun, Fan Lina, Li Qiwei, Qi Yongwen. 2017. Cloning and expression analysis of potassium transporter SsHAK2 in sugarcane(Saccharum species hybrid). Journal of Agricultural Biotechnology, 25 (3):378-385. (in Chinese)
	凌秋平, 曾巧英, 胡斐, 吴嘉云, 樊丽娜, 李奇伟, 齐永文. 2017. 甘蔗钾转运蛋白基因SsHAK2的克隆及表达特性分析. 农业生物技术学报, 25 (3):378-385.
[20]	Ma Li-juan, Ma Yu, He Hong-hong, Liang Guo-ping, Wang Peng, Chen Bai-hong, Mao Juan. 2019. Identification and expression analysis of grape HAK gene family. Acta Agriculturae Boreali-Occidentalis Sinica, 28 (6):922-934. (in Chinese)
	马丽娟, 马钰, 何红红, 梁国平, 万鹏, 陈佰鸿, 毛娟. 2019. 葡萄HAK基因家族的鉴定与表达分析. 西北农业学报, 28 (6):922-934.
[21]	Ma Li-ying. 2011. Comparative analysis of HAK/KUP/KT gene family in Arabidopsis thaliana and PaHAK1 in Phytolacca acinosa[M. D. Dissertation]. Changsha: Hunan Agricultural University. (in Chinese)
	马立英. 2011. 商陆高亲和性K⁺转运体基因PaHAK1的克隆及其功能的初步分析[博士论文]. 长沙: 湖南农业大学.
[22]	Maathuis F J M. 2006. The role of monovalent cation transporters in plant responses to salinity. Journal of Experimental Botany, 57 (5):1137-1147. pmid: 16263900
[23]	Magali L. 2002. PlantCARE,a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Research, 30 (1):325-327.
[24]	Mäser P, Thomine S, Schroeder J I, Ward J M, Guerinot M L. 2001. Phylogenetic relationships within cation transporter families of arabidopsis1. Plant Physiology, 126 (4):1646-1667. pmid: 11500563
[25]	Nieves-Cordones M, Alemán F, Martínez V, Rubio F. 2010. The arabidopsis thaliana HAK 5 K⁺ transporter is required for plant growth and K⁺ acquisition from low K⁺ solutions under saline conditions. Molecular Plant, 3 (2):326-333. doi: 10.1093/mp/ssp102 pmid: 20028724
[26]	Osakabe Y, Arinaga N, Umezawa T, KatsuraS, Nagamachi K, Tanaka H, Ohiraki H, Yamada K, Seo SU, Abo M. 2013. Osmotic stress responses and plant growth controlled by potassium transporters in Arabidopsis. Plant Cell, 25 (2):609-624. doi: 10.1105/tpc.112.105700 URL
[27]	Qi J G, Sun S M, Yang L, Li M J, Ma F W, Zou Y J. 2019. Potassium uptake and transport in apple roots under drought stress. Horticultural Plant Journal, 5 (1):10-16. doi: 10.1016/j.hpj.2018.10.001 URL
[28]	Qi Z, Hampton C R, Ryoung S, Barkla B J, White P J, Schachtman D P. 2008. The high affinity K⁺ transporter AtHAK 5 plays a physiological role in planta at very low K⁺ concentrations and provides a caesium uptake pathway in Arabidopsis. Journal of Experimental Botany, 59 (3):595-607. doi: 10.1093/jxb/erm330 URL
[29]	Qin G H, Ming C Y, Tang H B, Guyot, 2017. The pomegranate(Punica granatum L.)genome and the genomics of punicalagin biosynthesis. Plant Journal, 91 (6):1108-1128. doi: 10.1111/tpj.13625 URL
[30]	Quintero F J, Garciadeblás B, Rodríguez-Navarro A. 1996. The SAL 1 gene of Arabidopsis,encoding an enzyme with 3'(2'),5'-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities,increases salt tolerance in yeast. Plant Cell, 8 (3):529-537. pmid: 8721754
[31]	Rodríguez-Navarro A. 2000. Potassium transport in fungi and plants. Biochimica Et Biophysica Acta, 1469 (1):1-30. pmid: 10692635
[32]	Rubio F, Guillermo E Santa-aría, Alonso Rodríguez-Navarro. 2010. Cloning of Arabidopsis and barley cDNAs encoding HAK potassium transporters in root and shoot cells. Physiologia Plantarum, 109 (1):34-43. doi: 10.1034/j.1399-3054.2000.100106.x URL
[33]	Santa-Maria G E, Rubio F, Dubcovsky J, Rodriguez-Navarro A. 1998. The HAK 1 gene of barley is a member of a large gene family and encodes a high-affinity potassium transporter. The Plant Cell, 9 (12):2281-2289.
[34]	Shen Y, Shen L, Shen Z, Jing W, Ge H, Zhao J, Zhang W. 2015. The potassium transporter OsHAK 21 functions in the maintenance of ion homeostasis and tolerance to salt stress in rice. Plant Cell & Environment, 38 (12):2766-2799.
[35]	Shen Yue. 2015. Functional analyses ofpotassium transporter OsHAK21 and channel OsKs in response to salt stress in rice[Ph. D. Dissertation]. Nanjing:Nanjing Agricultural University. (in Chinese)
	沈悦. 2015. 水稻钾转运蛋白OsHAK21和通道蛋白OsKx响应盐胁迫的功能研究[博士论文]. 南京: 南京农业大学.
[36]	Rigas S, Ditengou F A, Ljung K, Daras G, Tietz O, Palme K, Hatzopoulos P. 2012. Root gravitropism and root hair development constitute coupled developmental responses regulated by auxin homeostasis in the Arabidopsis root apex. New Phytologist, 197 (4):1130-1141. doi: 10.1111/nph.12092 URL
[37]	Véry A-A, Nieves-Cordones M, Daly M, Khan I, Fizames C, Sentenac H. 2014. Molecular biology of K⁺ transport across the plant cell membrane:what do we learn from comparison between plant species? Journal of Plant Physiology, 171 (9):748-769.
[38]	Vicente-Agullo F, Rigas S, Desbrosses G, Dolan L, Grabov A. 2004. Potassium carrier TRH 1 is required for auxin transport in Arabidopsis roots. Plant Journal for Cell & Molecular Biology, 40 (4):523-535.
[39]	Wang Y, Wu W H. 2013. Potassium transport and signaling in higher plants. Annual Review of Plant Biology, 64 (1):451-476. doi: 10.1146/annurev-arplant-050312-120153 URL
[40]	Wang Y Z, Lv J H, Chen D, Zhang J, Qi K J, Cheng R, Zhang H P, Zhang S L. 2018. Genome-wide identification,evolution,and expression analysis of the KT/HAK/KUP family in pear. Genome,61: gen-2017-0254.
[41]	Yang T, Zhang S, Hu Y, Wu F, Hu Q, Chen G, Cai J, Wu T, Moran N, Yu L. 2014. The role of a potassium transporter OsHAK 5 in potassium acquisition and transport from roots to shoots in rice at low potassium supply levels. Plant Physiology, 166 (2):945-959. doi: 10.1104/pp.114.246520 URL
[42]	Youn-Jeong N, Phan L S, Mikiko K, Hitoshi S, Rie N, Ryoung S, Ivan B. 2012. Regulatory roles of cytokinins and cytokinin signaling in response to potassium deficiency in Arabidopsis. PLoS One, 7 (10):e47797. doi: 10.1371/journal.pone.0047797 URL
[43]	Zhang Z, Zhang J, Chen Y, Li R, Wei J. 2012. Genome-wide analysis and identification of HAK potassium transporter gene family in maize(Zea mays L.). Molecular Biology Reports, 39 (8):8465-8473. doi: 10.1007/s11033-012-1700-2 URL

名称 Name	基因号 ID	染色体号 Chr.	氨基酸 aa	蛋白质分子量/kD MW	等电点 pI	不稳定系数II Coefficient of instability	跨膜区 Transmem- brane domain	亚细胞定位 Subcellular localization
PgrHAK1	Pgr022478.1	Chr02	788	88.31	7.58	40.47	13	液泡膜 Vacuolar
PgrHAK2	Pgr025731.1	Chr09	798	88.95	7.87	40.61	13	液泡膜 Vacuolar
PgrHAK3	Pgr028419.1	Chr03	786	87.46	8.84	44.52	12	液泡膜 Vacuolar
PgrHAK4	Pgr017987.1	Chr05	745	83.65	9.15	40.44	12	质膜 Plasma membrane
PgrHAK5	Pgr009913.1	scaffold21	810	90.23	8.63	34.14	12	质膜 Plasma membrane
PgrHAK6	Pgr024378.1	Chr01	783	87.91	8.49	41.77	12	液泡膜 Vacuolar
PgrHAK7	Pgr016451.1	Chr06	757	84.37	5.12	41.43	10	质膜 Plasma membrane
PgrHAK8	Pgr014342.1	Chr07	779	87.06	8.00	37.49	10	液泡膜 Vacuolar
PgrHAK9	Pgr008140.1	Chr09	808	90.09	5.66	41.94	12	液泡膜 Vacuolar
PgrHAK10	Pgr027044.1	Chr03	794	89.00	7.31	42.90	13	液泡膜 Vacuolar
PgrHAK11	Pgr029120.1	Chr07	800	89.40	8.62	35.17	13	质膜 Plasma membrane
PgrHAK12	Pgr009693.1	scaffold21	837	92.45	5.46	43.09	12	液泡膜 Vacuolar
PgrHAK13	Pgr009692.3	scaffold21	697	78.48	8.56	41.39	10	质膜 Plasma membrane
PgrHAK14	Pgr018572.1	Chr08	772	87.36	6.55	36.03	11	液泡膜 Vacuolar
PgrHAK15	Pgr023110.1	Chr03	689	77.41	8.75	32.10	12	质膜 Plasma membrane
PgrHAK16	Pgr011385.2	Chr05	757	84.26	8.86	34.05	12	质膜 Plasma membrane
PgrHAK17	Pgr011384.1	Chr05	799	89.40	8.92	38.17	12	质膜 Plasma membrane
PgrHAK18	Pgr026837.2	Chr01	695	77.11	8.12	41.49	12	质膜 Plasma membrane

名称 Name	基因号 ID	染色体号 Chr.	氨基酸 aa	蛋白质分子量/kD MW	等电点 pI	不稳定系数II Coefficient of instability	跨膜区 Transmem- brane domain	亚细胞定位 Subcellular localization
PgrHAK1	Pgr022478.1	Chr02	788	88.31	7.58	40.47	13	液泡膜 Vacuolar
PgrHAK2	Pgr025731.1	Chr09	798	88.95	7.87	40.61	13	液泡膜 Vacuolar
PgrHAK3	Pgr028419.1	Chr03	786	87.46	8.84	44.52	12	液泡膜 Vacuolar
PgrHAK4	Pgr017987.1	Chr05	745	83.65	9.15	40.44	12	质膜 Plasma membrane
PgrHAK5	Pgr009913.1	scaffold21	810	90.23	8.63	34.14	12	质膜 Plasma membrane
PgrHAK6	Pgr024378.1	Chr01	783	87.91	8.49	41.77	12	液泡膜 Vacuolar
PgrHAK7	Pgr016451.1	Chr06	757	84.37	5.12	41.43	10	质膜 Plasma membrane
PgrHAK8	Pgr014342.1	Chr07	779	87.06	8.00	37.49	10	液泡膜 Vacuolar
PgrHAK9	Pgr008140.1	Chr09	808	90.09	5.66	41.94	12	液泡膜 Vacuolar
PgrHAK10	Pgr027044.1	Chr03	794	89.00	7.31	42.90	13	液泡膜 Vacuolar
PgrHAK11	Pgr029120.1	Chr07	800	89.40	8.62	35.17	13	质膜 Plasma membrane
PgrHAK12	Pgr009693.1	scaffold21	837	92.45	5.46	43.09	12	液泡膜 Vacuolar
PgrHAK13	Pgr009692.3	scaffold21	697	78.48	8.56	41.39	10	质膜 Plasma membrane
PgrHAK14	Pgr018572.1	Chr08	772	87.36	6.55	36.03	11	液泡膜 Vacuolar
PgrHAK15	Pgr023110.1	Chr03	689	77.41	8.75	32.10	12	质膜 Plasma membrane
PgrHAK16	Pgr011385.2	Chr05	757	84.26	8.86	34.05	12	质膜 Plasma membrane
PgrHAK17	Pgr011384.1	Chr05	799	89.40	8.92	38.17	12	质膜 Plasma membrane
PgrHAK18	Pgr026837.2	Chr01	695	77.11	8.12	41.49	12	质膜 Plasma membrane

石榴HAK/KUP/KT家族基因鉴定及钾转运功能分析

Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate

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石榴HAK/KUP/KT家族基因鉴定及钾转运功能分析

Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate

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