抗小西葫芦黄花叶病毒的西瓜自交系抗性遗传特征研究

doi:10.16420/j.issn.0513-353x.2021-0067

摘要/Abstract

摘要：

小西葫芦黄花叶病毒（zucchini yellow mosaic virus,ZYMV）严重危害西瓜等葫芦科作物。西瓜938-16-B是对ZYMV产生过敏性坏死反应（HR）的抗性自交系。为研究其对ZYMV的抗性遗传特征,以其为父本与感病亲本H1杂交,构建了包含310个单株的F₂群体及BC₁群体,通过对亲本、F₁、BC₁和F₂的抗病性鉴定,分析938-16-B的抗性遗传规律。结果发现：温度对西瓜幼苗感染ZYMV后HR的形成有一定影响,低温（< 28 ℃）下比高温（> 30 ℃）更易产生HR反应。在严格控制温度（23 ~ 28 ℃）的条件下对杂交后代群体进行接种抗病性鉴定,结果显示,F₂群体的抗、感分离比符合3︰1（χ²= 0.520,P = 0.470）;BC₁的抗、感比例符合1︰1（χ²= 0.040,P = 0.841）,而亲本938-16-B和F₁全部抗病,亲本H1全部感病,表明西瓜938-16-B抗ZYMV为单基因显性遗传。

关键词: 西瓜, 小西葫芦黄花叶病毒, 抗性, 遗传规律

Abstract:

Zucchini yellow mosaic virus is a serious emerging virus that has threatened the economically important cucurbits industry in major production areas worldwide. Watermelon inbred line 938-16-B had been identified as resistance to ZYMV in our previous work. It is characterized by hypersensitive response（HR）after infection by ZYMV. Here,we investigated the inheritance of resistance to ZYMV in 938-16-B. In this study,F₂ generation with 310 individuals and BC₁ generation were generated through crossing 938-16-B with ZYMV-susceptible inbred line H1. According to the segregation ratio of resistance in BC₁ and F₂ generation,ZYMV resistance in 938-16-B would be established. During the process of inoculation,we found hypersensitive response（HR）would not be induced when the temperature was over 30 ℃,which suggested the resistant gene was temperature-sensitive. Therefore,identification of resistance in the F₂ and BC₁ population was carried out under the condition of 23-28 ℃. Results showed that,the segregation ratios of resistance to susceptibility in F₂ and BC₁ were 3︰1 and 1︰1,respectively,which revealed the resistance to ZYMV in watermelon is controlled by a single dominant gene.

Key words: watermelon, zucchini yellow mosaic virus, hypersensitive response, genetic law

中图分类号:

S651

白二俊, 刘莉铭, 郝小苑, 彭斌, 吴会杰, 古勤生, 康保珊. 抗小西葫芦黄花叶病毒的西瓜自交系抗性遗传特征研究[J]. 园艺学报, 2022, 49(3): 633-640.

BAI Erjun, LIU Liming, Hao Xiaoyuan, PENG Bin, WU Huijie, GU Qinsheng, KANG Baoshan. Analysis on the Resistance to Zucchini Yellow Mosaic Virus in Watermelon Inbred Line 938-16-B[J]. Acta Horticulturae Sinica, 2022, 49(3): 633-640.

图/表 5

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群体 Population	光照强度/% Light intensity	总株数 Total	抗病株 Resistant plant
F₂-9	60 ~ 100	9	7
	20 ~ 60	8	7
	13 ~ 20	10	影响生长 Growth being limited
F₂-10	60 ~ 100	7	6
	20 ~ 60	8	7
	13 ~ 20	10	影响生长 Growth being limited

群体 Population	光照强度/% Light intensity	总株数 Total	抗病株 Resistant plant
F₂-9	60 ~ 100	9	7
	20 ~ 60	8	7
	13 ~ 20	10	影响生长 Growth being limited
F₂-10	60 ~ 100	7	6
	20 ~ 60	8	7
	13 ~ 20	10	影响生长 Growth being limited

群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plant	群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plant
F₂-1	30 ~ 40	9	2	F₂-6	30 ~ 40	9	4
	23 ~ 28	5	5		23 ~ 28	8	8
F₂-2	30 ~ 40	9	2	F₂-7	30 ~ 40	9	2
	23 ~ 28	8	5		23 ~ 28	9	7
F₂-3	30 ~ 40	9	4	F₂-8	30 ~ 40	8	1
	23 ~ 28	6	6		23 ~ 28	7	4
F₂-4	30 ~ 40	8	3	F₂-9	30 ~ 40	10	0
	23 ~ 28	8	7		23 ~ 28	9	7
F₂-5	30 ~ 40	9	1	F₂-10	30 ~ 40	10	0
	23 ~ 28	8	7		23 ~ 28	7	6

群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plant	群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plant
F₂-1	30 ~ 40	9	2	F₂-6	30 ~ 40	9	4
	23 ~ 28	5	5		23 ~ 28	8	8
F₂-2	30 ~ 40	9	2	F₂-7	30 ~ 40	9	2
	23 ~ 28	8	5		23 ~ 28	9	7
F₂-3	30 ~ 40	9	4	F₂-8	30 ~ 40	8	1
	23 ~ 28	6	6		23 ~ 28	7	4
F₂-4	30 ~ 40	8	3	F₂-9	30 ~ 40	10	0
	23 ~ 28	8	7		23 ~ 28	9	7
F₂-5	30 ~ 40	9	1	F₂-10	30 ~ 40	10	0
	23 ~ 28	8	7		23 ~ 28	7	6

群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plants	群体 Population	温度/℃ Temperature	总株数 Total	抗病株数 Resistant plants
P₁(938-16-B)	30 ~ 35	9	7	F₂-9	30 ~ 35	14	1
	23 ~ 28	10	10		23 ~ 28	9	7
	18 ~ 23	11	11		18 ~ 23	10	9
F₁	30 ~ 35	9	2	F₂-10	30 ~ 35	19	0
	23 ~ 28	5	5		23 ~ 28	7	6
	18 ~ 23	2	2		18 ~ 23	6	6