西州密系列甜瓜SSR指纹图谱构建及聚类分析

doi:10.16420/j.issn.0513-353x.2021-0267

摘要/Abstract

摘要：

为利用SSR标记技术建立西州密系列甜瓜品种的DNA指纹图谱,实现快速准确地鉴定品种。利用18份西州密系列甜瓜品种（系）对73对甜瓜SSR引物进行筛选,获得9对扩增稳定、呈共显性、条带清晰、多态性好的引物,其中每对引物可检测到3 ~ 6个等位变异,平均4.33个;多态性信息含量（PIC）平均值为0.45,变化范围为0.20 ~ 0.78。利用C30、MU5499、MU5554-1、SSR04219、SSR12083等5对SSR核心引物有效区分所有供试材料,并为每份材料建立了一份独特的指纹图谱,其中‘西州密25号’母本与‘西州密17号’母本为同一材料。同时对18份材料进行了聚类分析,其相似系数为0.70 ~ 1.00。在相似性系数0.74处,可将供试材料分为三大类群。SSR引物适用于构建西州密系列甜瓜品种（系）的DNA指纹图谱,可为其品种鉴定提供科学依据。

关键词: 厚皮甜瓜, SSR, 指纹图谱, 聚类分析

Abstract:

In order to identify the cultivars quickly and accurately,DNA fingerprinting of 18 muskmelon cultivars of Xizhoumi series was constructed by using SSR marker technology. Using 18 cultivars（lines）,highly informative and polymorphic simple sequence repeat（SSR）primers were screened. Out of the 73 SSR primer pairs,nine pairs including SSR12083,MU5554-1,TJ10,PM3,MU5499,SSR04219,SSR04632,SSR22874 and C30 were selected,and they could generate stable,clearly and codominant bands. Each primer pair could generate 3-6 polymorphic bands with an average of 4.33. The average polymorphism information content（PIC）was 0.45 with a range from 0.20 to 0.78. Five selected primer pairs（C30,MU5499,MU5554-1,SSR04219 and SSR12083）had established the fingerprinting of the 18 accessions and their unique fingerprint. We found that the female parent of ‘Xizhoumi 25’was the same as the female parent of‘Xizhoumi 17’. Clustering analysis showed that the similarity coefficient of the 18 accessions ranged from 0.70 to 1.00. They can be divided into three groups at 0.74. Above results indicated that the DNA fingerprinting could provide scientific basis for identification of Xizhoumi muskmelon.

Key words: muskmelon, SSR, fingerprinting, clustering analysis

中图分类号:

S652

李超, 杨英, 陈伟, 郑贺云, 廖新福, 孙玉萍. 西州密系列甜瓜SSR指纹图谱构建及聚类分析[J]. 园艺学报, 2022, 49(3): 622-632.

LI Chao, YANG Ying, CHEN Wei, ZHENG Heyun, LIAO Xinfu, SUN Yuping. Construction of DNA Fingerprinting and Clustering Analysis with SSR Markers for the Muskmelon of Xizhoumi Series[J]. Acta Horticulturae Sinica, 2022, 49(3): 622-632.

图/表 7

参考文献 34

[1]	Ai Cheng-xiang, Yu Xian-mei, Ma Guo-bin, Liu Qing-zhong. 2006. Establishment of fingerprinting for melon hybrids by SSR markers. Journal of Fruit Science, 23(3):415-419. (in Chinese)
	艾呈祥, 余贤美, 马国斌, 刘庆忠. 2006. 甜瓜杂交种指纹图谱的构建. 果树学报, 23(3):415-419.
[2]	Chen Liang, Zheng Yu-hong, Fan Xu-hong, Meng Fan-Fan, Sun Xing-miao, Zhang Yun-feng, Wang Ming-liang, Wang Shu-yue. 2016. Establishment of SSR fingerprint ID for new soybean varieties in Jilin. Soybean Science, 35(6):896-901. (in Chinese)
	陈亮, 郑宇宏, 范旭红, 孟凡凡, 孙星邈, 张云峰, 王明亮, 王曙月. 2016. 吉林省新育成大豆品种SSR指纹图谱身份证的构建. 大豆科学, 35(6):896-901.
[3]	Cheng Meng-jie, Yan Shuang-yong, Shi Li-li, Sun Ning, Zhang Xin, Ding De-liang, Bian Jia-bin, Wang Song-wen. 2018. Evaluation of rice variety polymorphisms using KASP markers. Journal of Tianjin Agricultural University, 25(4):13-23. (in Chinese)
	程萌杰, 闫双勇, 施利利, 孙宁, 张欣, 丁得亮, 边嘉宾, 王松文. 2018. 利用KASP标记评价水稻品种多态性. 天津农学院学报, 25(4):13-23.
[4]	Cheng Xunhui, Xie Zhao, Yang Zhongzhou, Dai Wei, Dai Zuyun, Song Jianghua. 2021. A new melon cultivar‘Jianghuaimi 7’. Acta Horticulturae Sinica, 48(9):1841-1842. (in Chinese)
	程勋辉, 谢钊, 杨中周, 代伟, 戴祖云, 宋江华. 2021. 甜瓜新品种‘江淮蜜7号’. 园艺学报, 48(9):1841-1842.
[5]	Duan Yan-feng, Liu Jie, Bian Chun-song, Duan Shao-guang, Xu Jian-fei, Jin Li-ping. 2009. Construction of fingerprinting and analysis of genetic diversity with SSR marker for eighty-eight approved potato cultivars(Solanum tuberosum L.). Acta Agronomica Sinica, 35(8):1451-1457. (in Chinese) doi: 10.3724/SP.J.1006.2009.01451 URL
	段艳凤, 刘杰, 卞春松, 段绍光, 徐建飞, 金黎平. 2009. 中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析. 作物学报, 35(8):1451-1457.
[6]	Fan Jian-guang, Zhang Hai-ying, Gong Guo-yi, Xiao Jing, Guo Shao-gui, Ren Yi, Zhang Jie, Wang Xiu-feng, Ma Yan-ming, Xu Yong. 2013. Construction and application of SSR fingerprint database of the example varieties in watermelon DUS testing. Journal of Plant Genetic Resources, 14(5):892-899. (in Chinese)
	范建光, 张海英, 宫国义, 肖菁, 郭绍贵, 任毅, 张洁, 王秀峰, 马艳明, 许勇. 2013. 西瓜DUS测试标准品种SSR指纹图谱构建及应用. 植物遗传资源学报, 14(5):892-899.
[7]	Gao Peng, Liu Shi, Cui Haonan, Zhang Taifeng, Wang Xuezheng, Liu Hongyu, Zhu Zicheng, Luan Feishi. 2020. Research progress of melon genomics,functional gene mapping and genetic engineering. Acta Horticulturae Sinica, 47(9):1827-1844. (in Chinese)
	高鹏, 刘识, 崔浩楠, 张泰峰, 王学征, 刘宏宇, 朱子成, 栾非时. 2020. 甜瓜基因组学、功能基因定位及基因工程育种研究进展. 园艺学报, 47(9):1827-1844.
[8]	Gao Song, Liu Hong-wei, He Huan, Lü Qing-xue, Zhou De-long, Xing Yue-xian, Zhao Xing-yan, Zhang Zhi-jun, Zhang Jian-xin, Zhong Yi, Xia Yuan-feng, Song Guang-shu. 2021. Analysis on maize genetic diversity and population genetic structure by SNP chip & breeding of new maize varieties. Journal of Maize Sciences, 29(1):39-45. (in Chinese)
	高嵩, 刘宏伟, 何欢, 吕庆雪, 周德龙, 邢跃先, 赵兴彦, 张志军, 张建新, 仲义, 夏远峰, 宋广树. 2021. 利用SNP芯片进行玉米遗传多样性和群体遗传结构分析及新品种选育. 玉米科学, 29(1):39-45.
[9]	Gao Yuan, Wang Kun, Wang Da-jiang, Gong Xin, Liu Li-jun, Liu Feng-zhi. 2016. Molecular ID establishment of apple cultivars by TP-M13-SSR. Acta Horticulturae Sinica, 43(1):25-37. (in Chinese)
	高源, 王昆, 王大江, 龚欣, 刘立军, 刘凤之. 2016. 利用TP-M13-SSR标记构建苹果栽培品种的分子身份证. 园艺学报, 43(1):25-37.
[10]	Li Chao, Sun Yu-ping, Yang Ying, Xu Chang, Zhang Rui, Liao Xin-fu. 2017. Application of SSR markers in purity and authenticity identification for melon new hybrid cultivars. Molecular Plant Breeding, 15(8):3088-3096. (in Chinese)
	李超, 孙玉萍, 杨英, 徐畅, 张瑞, 廖新福. 2017. 应用SSR标记鉴定甜瓜新品种纯度与真实性研究. 分子植物育种, 15(8):3088-3096.
[11]	Li Chao, Sun Yu-ping, Yang Ying, Zheng He-yun, Chen Wei, Liao Xin-fu. 2019. Screening of SSR primers for seed purity identification on Cucumis melon ssp. melo breed combination. Molecular Plant Breeding, 17(16):5347-5356. (in Chinese)
	李超, 孙玉萍, 杨英, 郑贺云, 陈伟, 廖新福. 2019. 厚皮甜瓜品种组合种子纯度鉴定SSR引物的筛选. 分子植物育种, 17(16):5347-5356.
[12]	Li Rui-feng. 2014. Construction of DNA fingerprint and analysis of genetic diversity for varieties of melon(Cucumis melo L.)[M. D. Dissertation]. Harbin: Northeast Agricultural University. (in Chinese)
	李瑞峰. 2014. 甜瓜主栽品种指纹图谱库的构建及遗传多样性分析[硕士论文]. 哈尔滨: 东北农业大学.
[13]	Li Zhi-yuan. 2018. DNA fingerprinting and heterotic group classification by using KASP marker in cabbage(Brassica oleracea L. var. capitata)[M. D. Dissertation]. Beijing: Chinese Academy of Agricultural Sciences. (in Chinese)
	李志远. 2018. KASP标记用于甘蓝指纹图谱构建及杂种优势群划分[硕士论文]. 北京: 中国农业科学院.
[14]	Lu Fang, Xu Yong, Zhao Yue, Cao Di, Feng Jian-ming, Guo Shao-gui, Gong Guo-yi, Yi Hong-ping, Wu Ming-zhu, Zhang Hai-ying. 2009. Construction of permanent genetic map and comparative analysis of Xinjiang Hami melon [Cucumis melo L. ssp. melo convar. ameri(Pang.)Greb.]. Acta Horticulturae Sinica, 36(12):1767-1774. (in Chinese)
	陆芳, 许勇, 赵越, 曹迪, 冯建明, 郭绍贵, 宫国义, 伊鸿平, 吴明珠, 张海英. 2009. 新疆哈密瓜永久遗传图谱构建及比较分析. 园艺学报, 36(12):1767-1774.
[15]	Ning Qia, Liu Wen-guo, Yang Wei-guang, Lu Ming. 2017. Progress and application of SNP markers in maize. Journal of Maize Sciences, 25(1):57-61. (in Chinese)
	宁洽, 刘文国, 杨伟光, 路明. 2017. SNP标记在玉米研究上的应用进展. 玉米科学, 25(1):57-61.
[16]	Ouni R, Zborowska A, Sehic J, Choulak S, Hormaza J I, Garkava-Gustavsson L, Mars M. 2020. Genetic diversity and structure of Tunisian local pear germplasm as revealed by SSR markers. Horticultural Plant Journal, 6(2):61-70. doi: 10.1016/j.hpj.2020.03.003 URL
[17]	Shen Jia, Shou Wei-song, Zhang Yue-jian. 2015. The QR code construction and seed purity detection in molecular technology of melon Cuixue No.5. Acta Agriculturae Zhejiangensis, 56(12):1946-1949. (in Chinese)
	沈佳, 寿伟松, 张跃建. 2015. 甜瓜翠雪5号的二维码构建及种子纯度分子检测技术. 浙江农业学报, 56(12):1946-1949.
[18]	Song Hai-bin, Cui Xi-bo, Ma Hong-yan, Zhu Zi-cheng, Luan Fei-shi. 2012. Construction of DNA fingerprint database based on SSR marker for varieties(lines)of Cucumis melo L. Scientia Agricultura Sinica, 45(13):2676-2689. (in Chinese)
	宋海斌, 崔喜波, 马鸿艳, 朱子成, 栾非时. 2012. 基于SSR标记的甜瓜品种(系)DNA指纹图谱库的构建. 中国农业科学, 45(13):2676-2689.
[19]	Wang Fu-qiang, Fan Xiu-cai, Zhang Ying, Liu Chong-huai, Jiang Jian-fu. 2020. Application and prospect of SNP molecular markers in crop variety identification. Journal of Plant Genetic Resources, 21(5):1308-1320. (in Chinese)
	王富强, 樊秀彩, 张颖, 刘崇怀, 姜建福. 2020. SNP分子标记在作物品种鉴定中的应用和展望. 植物遗传资源学报, 21(5):1308-1320.
[20]	Wang Mei-rong, Xu Yong, Zhan Yong-le, Guo Shao-gui, Ren Yi, Gong Guo-yi, Zhang Hai-ying. 2010a. Construction of fingerprinting with SSR for varieties of Cucumis melo ssp. melo. Chinese Agricultural Science Bulletin, 26(20):47-51. (in Chinese)
	王美荣, 许勇, 詹永乐, 郭绍贵, 任毅, 宫国义, 张海英. 2010a. 厚皮甜瓜品种组合SSR指纹图谱构建. 中国农学通报, 26(20):47-51.
[21]	Wang Mei-rong, Xu Yong, Zhan Yong-le, Guo Shao-gui, Ren Yi, Gong Guo-yi, Zhang Hai-ying. 2010b. Analysis of genetic diversity and population structure in melon(Cucumis melo L.)breeding materials by SSR markers. Acta Agricultural Boreali-Sinica, 25(Supplement):41-46. (in Chinese)
	王美荣, 许勇, 詹永乐, 郭绍贵, 任毅, 宫国义, 张海英. 2010b. 甜瓜育种亲本材料遗传多样性及群体结构的SSR标记分析. 华北农学报, 25(S):41-46.
[22]	Wang Jin, You Jia, Wang Xi-qiao, Wen Zhao-hui, Wang Jun-ping. 2015. Establishment of SSR fingerprint on melon hybrids and their parents. Acta Agriculturae Boreali-Sinica, 30(Supplement):66-68. (in Chinese)
	王晋, 尤佳, 王溪桥, 文朝慧, 王军平. 2015. 甜瓜品种SSR指纹图谱的构建. 华北农学报, 30(S):66-68.
[23]	Wang Rui, Shi Long-Jian, Tian Hong-li, Yi Hong-mei, Yang Yang, Ge Jian-rong, Fan Ya-ming, Ren Jie, Wang Lu, Lu Da-lei, Zhao Jiu-ran, Wang Feng-ge. 2021. Acta Agronomica Sinica, 47(4):770-779. (in Chinese) doi: 10.3724/SP.J.1006.2021.03031 URL
	王蕊, 施龙建, 田红丽, 易红梅, 杨扬, 葛建镕, 范亚明, 任洁, 王璐, 陆大雷, 赵久然, 王凤格. 2021. 玉米杂交种纯度鉴定SNP核心引物的确定及高通量检测法案的建立. 作物学报, 47(4):770-779.
[24]	Wang Xian-lei, Gao Xing-wang, Li Guan, Wang Hui-lin, Geng Shou-dong, Kang Feng, Nie Xiang-xiang. 2011. Construction of a melon genetic map with fruit and seed QTLs. Hereditas, 33(12):1398-1408. (in Chinese)
	王贤磊, 高兴旺, 李冠, 王慧林, 耿守东, 康锋, 聂祥祥. 2011. 甜瓜遗传图谱的构建及果实与种子QTL分析. 遗传, 33(12):1398-1408.
[25]	Wu Sheng-nan, Li Ying-zhuang, Wang Na, Liu Lu-xiang, Xie Yan-zhou, Wang Cheng-she. 2021. Dissection of genetic components in the new high-yielding wheat cultivar Shaannong 33. Journal of Triticeae Crops, 41(2):1-6. (in Chinese)
	吴胜男, 李英壮, 王娜, 刘录祥, 谢彦周, 王成社. 2021. 小麦品种陕农33的遗传构成分析. 麦类作物学报, 41(2):1-6.
[26]	Xu Yun-bi, Yang Quan-nü, Zheng Hong-Jian, Xu Yan-fen, Sang Zhi-qin, Guo Zi-feng, Peng Hai, Zhang Cong, Lan Hao-fa, Wang Yun-bo, Wu Kun-sheng, Tao Jia-jun, Zhang Jia-nan. 2020. Genotyping by target sequencing(GBTS)and its applications. Scientia Agricultura Sinica, 53(15):2983-3004. (in Chinese)
	徐云碧, 杨泉女, 郑洪建, 许彦芬, 桑志勤, 郭子峰, 彭海, 张丛, 蓝昊发, 王蕴波, 吴坤生, 陶家军, 张嘉楠. 2020. 靶向测序基因型检测(GBTS)技术及其应用. 中国农业科学, 53(15):2983-3004.
[27]	Xue Y Q, Liu R, Xue J Q, Wang S L, Zhang X X. 2021. Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers. Horticultural Plant Journal, 7(6):579-588. doi: 10.1016/j.hpj.2021.05.004 URL
[28]	Yang Mi. 2014. The effects of weak light environment on plants morpholgical and biochemical character of Xinjiang muskmelon[M. D. Dissertation]. Xinjiang: Xinjiang Agricultural University. (in Chinese)
	杨咪. 2014. 弱光环境对新疆甜瓜植株形态和生理特性的影响[硕士论文]. 新疆: 新疆农业大学.
[29]	Yi Li-cong, Wang Yun-qiang, Jiao Chun-hai, Yao Ming-hua, Gong Yu, Wang Shu-jing, Dai Zhao-yi. 2020. Genetic diversity analysis of 64 watermelon germplasms by SNP markers. China Cucurbits and Vegetables, 33(12):8-13. (in Chinese)
	易丽聪, 王运强, 焦春海, 姚明华, 龚钰, 王舒景, 戴照义. 2020. 基于SNP标记的西瓜种质资源遗传多样性分析. 中国瓜菜, 33(12):8-13.
[30]	Zhang Di, Ren Li-fei, Zhang Lu-lu, Zhang Wen-hao, Wang Tian-zuo. 2020. Construction of SSR fingerprint of Alfalfa based on fluorescent capillary electrophoresis. Chinese Journal of Grassland, 11:10-14. (in Chinese)
	张迪, 任立飞, 张璐璐, 张文浩, 王天佐. 2020. 基于荧光毛细管电泳技术的苜蓿SSR指纹图谱构建. 中国草地学报, 11:10-14.
[31]	Zhang Kai-ge, Hu Qian-mei, Jin Zhi-heng, Yang Hui-hui, Yang Lu-ming, Hu Jian-bin, Zhu Hua-yu. 2020. Genetic diversity analysis of 219 melon germplasms. Journal of Henan Agricultural University, 54(2):216-230. (in Chinese)
	张凯歌, 胡倩梅, 靳志恒, 杨会会, 杨路明, 胡建斌, 朱华玉. 2020. 219份甜瓜种质资源的遗传多样性分析. 河南农业大学学报, 54(2):216-230.
[32]	Zhang Rui, Liao Xin-fu, Yang Jun, Sun Yu-Ping, Yang Ying. 2012. Breeding of a new Hami melon variety‘Xizhoumi No. 25’. China Cucurbits and Vegetables, 25(3):20-23. (in Chinese)
	张瑞, 廖新福, 杨军, 孙玉萍, 杨英. 2012. 优质哈密瓜新品种西州密25号的选育, 中国瓜菜, 25(3):20-23.
[33]	Zhang Rui, Sun Yu-Ping, Yang Jun, Yang Ying, Hu Jin-ge, Liao Xin-fu. 2013. Breeding of a high quality melon variety‘Xizhoumi No. 17’. China Cucurbits and Vegetables, 26(1):29-31. (in Chinese)
	张瑞, 孙玉萍, 杨军, 杨英, 户金鸽, 廖新福. 2013. 优质甜瓜新品种西州密17号的选育. 中国瓜菜, 26(1):29-31.
[34]	Zhou Xian-da, Zhou Gui-lin, Fan Jia-meng, Sui Xiang-kun, Lin Mao, Wang Hao-bo. 2020. Study on the diversity and genetic relationship of sixty-nine accessions of Cucumis melo sp. conomon. China Cucurbits and Vegetables, 33(1):7-12. (in Chinese)
	周贤达, 周桂林, 范家萌, 睢祥坤, 林茂, 王浩波. 2020. 69份薄皮甜瓜材料的多样性及亲缘关系. 中国瓜菜, 33(1):7-12.

编号 Accession No.	材料名称 Material name	主要性状 Primary characteristics
编号 Accession No.	材料名称 Material name	果形 Fruit shape	熟性 Mature feature	中心糖/% Central sugar content
1	02-15（西州密1号母本 Female parent of Xizhoumi 1）	圆形 Round	早熟 Precocity	16.5
2	02-17（西州密1号父本 Male parent of Xizhoumi 1）	椭圆形 Oval	中熟 Mid-maturation	16.0
3	西州密1号 Xizhoumi 1	椭圆形 Oval	中早熟 Middle-early maturation	15.6 ~ 17.0
4	西州密3号母本 Female parent of Xizhoumi 3	短椭圆形 Short ellipse	中熟 Mid-maturation	16.8
5	西州密3号父本 Male parent of Xizhoumi 3	卵形 Ovoid	中熟 Mid-maturation	16.5
6	西州密3号 Xizhoumi 3	卵形 Ovoid	中早熟 Middle-early maturation	16.0 ~ 17.0
7	05-89（西州密25号母本 Female parent of Xizhoumi 25）	短椭圆形 Short ellipse	早中熟 Early-middle maturation	17.0
8	02-17（西州密25号父本 Male parent of Xizhoumi 25）	椭圆形 Oval	中熟 Mid-maturation	16.0
9	西州密25号 Xizhoumi 25	椭圆形 Oval	早中熟 Early-middle maturation	15.6 ~ 18.0
10	05-91（西州密17号母本 Female parent of Xizhoumi 17）	短椭圆形 Short ellipse	早中熟 Early-middle maturation	17.0
11	06-67（西州密17号父本 Male parent of Xizhoumi 17）	椭圆形 Oval	中熟 Mid-maturation	15.0
12	西州密17号 Xizhoumi 17	椭圆形 Oval	中晚熟 Middle-late maturation	15.2 ~ 17.0
13	08-20（西州密21号母本 Female parent of Xizhoumi 21）	椭圆形 Oval	中早熟 Middle-early maturation	18.0 ~ 20.0
14	08-26（西州密21号父本 Male parent of Xizhoumi 21）	椭圆形 Oval	中早熟 Middle-early maturation	17.0 ~ 19.0
15	西州密21号 Xizhoumi 21	椭圆形 Oval	中早熟 Middle-early maturation	18.0 ~ 20.0
16	西州密29号母本Female parent of Xizhoumi 29	椭圆形 Oval	早中熟 Early-middle maturation	17.0
17	西州密29号父本 Male parent of Xizhoumi 29	椭圆形 Oval	中熟 Mid-maturation	15.0 ~ 17.0
18	西州密29号 Xizhoumi 29	椭圆形 Oval	早中熟 Early-middle maturation	16.3 ~ 18.5

编号 Accession No.	材料名称 Material name	主要性状 Primary characteristics
编号 Accession No.	材料名称 Material name	果形 Fruit shape	熟性 Mature feature	中心糖/% Central sugar content
1	02-15（西州密1号母本 Female parent of Xizhoumi 1）	圆形 Round	早熟 Precocity	16.5
2	02-17（西州密1号父本 Male parent of Xizhoumi 1）	椭圆形 Oval	中熟 Mid-maturation	16.0
3	西州密1号 Xizhoumi 1	椭圆形 Oval	中早熟 Middle-early maturation	15.6 ~ 17.0
4	西州密3号母本 Female parent of Xizhoumi 3	短椭圆形 Short ellipse	中熟 Mid-maturation	16.8
5	西州密3号父本 Male parent of Xizhoumi 3	卵形 Ovoid	中熟 Mid-maturation	16.5
6	西州密3号 Xizhoumi 3	卵形 Ovoid	中早熟 Middle-early maturation	16.0 ~ 17.0
7	05-89（西州密25号母本 Female parent of Xizhoumi 25）	短椭圆形 Short ellipse	早中熟 Early-middle maturation	17.0
8	02-17（西州密25号父本 Male parent of Xizhoumi 25）	椭圆形 Oval	中熟 Mid-maturation	16.0
9	西州密25号 Xizhoumi 25	椭圆形 Oval	早中熟 Early-middle maturation	15.6 ~ 18.0
10	05-91（西州密17号母本 Female parent of Xizhoumi 17）	短椭圆形 Short ellipse	早中熟 Early-middle maturation	17.0
11	06-67（西州密17号父本 Male parent of Xizhoumi 17）	椭圆形 Oval	中熟 Mid-maturation	15.0
12	西州密17号 Xizhoumi 17	椭圆形 Oval	中晚熟 Middle-late maturation	15.2 ~ 17.0
13	08-20（西州密21号母本 Female parent of Xizhoumi 21）	椭圆形 Oval	中早熟 Middle-early maturation	18.0 ~ 20.0
14	08-26（西州密21号父本 Male parent of Xizhoumi 21）	椭圆形 Oval	中早熟 Middle-early maturation	17.0 ~ 19.0
15	西州密21号 Xizhoumi 21	椭圆形 Oval	中早熟 Middle-early maturation	18.0 ~ 20.0
16	西州密29号母本Female parent of Xizhoumi 29	椭圆形 Oval	早中熟 Early-middle maturation	17.0
17	西州密29号父本 Male parent of Xizhoumi 29	椭圆形 Oval	中熟 Mid-maturation	15.0 ~ 17.0
18	西州密29号 Xizhoumi 29	椭圆形 Oval	早中熟 Early-middle maturation	16.3 ~ 18.5

序号 Number	名称 Name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse Primer	等位变异数 Number of allelic variation	多样性指数 Diversity index
1	SSR12083	GAATTGGCCCATCCTTCATT	GCCATTCCAAAAACTTTTCAAC	5	0.38
2	MU5554-1	CCTTCATGATCCTCTACTAAACCC	TCTTCCATGCTTTTCTCGCT	6	0.71
3	TJ10	ACGAGGAAAACGCAAAATCA	TGAACGTGGACGACATTTTT	3	0.29
4	PM3	TCAACTGTCCATTTCTCGCTG	CCGTA AAGACGAAAACCCTTC	3	0.20
5	MU5499	AAGAAGAAAGTTCCCCTCTCG	CGACTGTGTCTTGTGGAGGA	6	0.78
6	SSR04219	GAGACATTGTGGGCATTTGA	CTCATTTTCATCCAAAGGGC	5	0.38
7	SSR04632	ACTCCGTTTGGTGGTTTTTG	TCCCAATTCCATAGCAGAGG	3	0.37
8	SSR22874	CAACCCCCAAGCTCTGATAA	ATTGGAGAGTGCTATTGGGG	3	0.20
9	C30	CTGTCTGCTATTCTCCACTTGG	TGTATGCCACGTAGCGAAAC	5	0.70

序号 Number	名称 Name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse Primer	等位变异数 Number of allelic variation	多样性指数 Diversity index
1	SSR12083	GAATTGGCCCATCCTTCATT	GCCATTCCAAAAACTTTTCAAC	5	0.38
2	MU5554-1	CCTTCATGATCCTCTACTAAACCC	TCTTCCATGCTTTTCTCGCT	6	0.71
3	TJ10	ACGAGGAAAACGCAAAATCA	TGAACGTGGACGACATTTTT	3	0.29
4	PM3	TCAACTGTCCATTTCTCGCTG	CCGTA AAGACGAAAACCCTTC	3	0.20
5	MU5499	AAGAAGAAAGTTCCCCTCTCG	CGACTGTGTCTTGTGGAGGA	6	0.78
6	SSR04219	GAGACATTGTGGGCATTTGA	CTCATTTTCATCCAAAGGGC	5	0.38
7	SSR04632	ACTCCGTTTGGTGGTTTTTG	TCCCAATTCCATAGCAGAGG	3	0.37
8	SSR22874	CAACCCCCAAGCTCTGATAA	ATTGGAGAGTGCTATTGGGG	3	0.20
9	C30	CTGTCTGCTATTCTCCACTTGG	TGTATGCCACGTAGCGAAAC	5	0.70

引物组合 Primer pairs	区分材料编号 Number of distinguished materials	区分开的材料小计 Subtotal of distinguished materials	区分开的材料累计 Total of distinguished materials
C30	11 + 12	2	2
C30 + Mu5499	14 + 15 + 18	3	5
C30 + Mu5499 + Mu5554-1	1 + 3 + 4 + 6 + 9 + 16 + 17	7	12
C30 + Mu5499 + Mu5554-1 + SSR04219	2 + 8	2	14
C30 + Mu5499 + Mu5554-1 + SSR04219 + SSR12083	5 + 13	2	16
C30 + Mu5499 + Mu5554-1 + SSR04219 + SSR12083 + …	7 + 10 ?	2	18