龙眼GDSL酯酶/脂肪酶基因的全基因组鉴定及表达分析

doi:10.16420/j.issn.0513-353x.2020-0942

摘要/Abstract

摘要：

基于第三代龙眼基因组数据库及其转录组数据库,通过生物信息学分析对龙眼GDSL酯酶/脂肪酶家族进行全基因组鉴定。结果显示龙眼GDSL（DlGDSL）家族共有118个成员,分为15个亚家族,亚细胞定位显示主要分布于细胞外基质中。染色体位置及共线性基因分析表明,118个DlGDSL基因中有111个定位在15条龙眼染色体上,包括13对共线性基因。基因结构和蛋白质保守基序分析表明该家族的外显子在2 ~ 12之间,且motif2和motif3是DlGDSL家族中尤为重要的保守基序。启动子顺式作用元件分析表明,启动子序列中包含众多光响应、激素应答、无氧诱导、胁迫响应和昼夜节律等响应元件,且大部分成员都含有茉莉酸甲酯（MeJA）、脱落酸（ABA）、生长素（IAA）和赤霉素（GA）等激素应答作用元件。FPKM值分析发现从胚性愈伤组织到球形胚阶段有26个差异表达的基因,提示DlGDSL家族部分成员可能在龙眼体胚形态建成中发挥重要作用。qRT-PCR分析表明,在外源ABA和MeJA处理下龙眼胚性愈伤组织中,DlGDSL家族部分成员可能与ABA、MeJA等激素信号转导相关。

关键词: 龙眼, GDSL, 全基因组, 鉴定, 表达分析

Abstract:

The evolution characteristics of the longan GDSL（DlGDSL）family and their expression patterns during the early stages of somatic embryogenesis were investigated. The results showed the 118 members of DlGDSL family can be divided into 15 subfamilies,and the subcellular localization was mainly distributed in the extracell,a few distributed in other parts such as cell membrane,chloroplast,mitochondria and endoplasmic reticulum. Analysis of chromosomal locations suggests that 111 out of 118 DlGDSLs are distributed unevenly on the 15 longan chromosomes. Analysis of synteny relationships indicates 13 pairs of DlGDSL genes. The genomic structures of those DlGDSL genes indicates that their exon numbers vary between two and twelve. The protein conserved motifs of those DlGDSL suggest motif2 and motif3 are particularly important. The cis-acting elements of DlGDSL promoters contained light responsive elements,hormone response,anaerobic induction,stress responsive and circadian control elements. Besides,three family members（A,B and C）of DlGDSL contains a large number of methyl jasmonate（MeJA）,abscisic acid（ABA）,auxin（IAA）and gibberellin（GA）action elements. In addition,transcriptome data analysis revealed that 26 differential expression genes from embryogenic callus to globular embryo stage,suggesting members of the DlGDSL family may play important roles in the morphogenesis of longan somatic embryos. The results of qRT-PCR analysis in different hormone treatments suggests some members of the DlGDSL family may be related to hormone signal transduction such as ABA and MeJA in longan embryogenic callus. DlGDSL57/69/105 genes showed obvious up-regulation and DlGDSL23/42/50/52/75/80/104 genes demonstrated visible down-regulation in ABA treatment. DlGDSL100 gene revealed obvious up-regulation and DlGDSL23/27/42/50/52/57/69/80/104/105 showed visible down-regulation in MeJA treatment. In summary,the research will provide a reference for the subsequent functional verification of DlGDSL genes in somatic embryogenesis.

Key words: Dimocarpus longan, GDSL, genome wide, indentification, expression analysis

中图分类号:

S667.2

刘梦雨, 蒋梦琦, 陈燕, 张舒婷, 薛晓东, 肖学宸, 赖钟雄, 林玉玲. 龙眼GDSL酯酶/脂肪酶基因的全基因组鉴定及表达分析[J]. 园艺学报, 2022, 49(3): 597-612.

LIU Mengyu, JIANG Mengqi, CHEN Yan, ZHANG Shuting, XUE Xiaodong, XIAO Xuechen, LAI Zhongxiong, LIN Yuling. Genome-wide Identification and Expression Analysis of GDSL Esterase/Lipase Genes in Dimocarpus longan[J]. Acta Horticulturae Sinica, 2022, 49(3): 597-612.

图/表 5

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基因ID Gene ID	基因名称 Gene name	开放阅读框/bp ORF	氨基酸数/aa Size	分子量/kD Molecular weight	等电点 pI	不稳定系数 The instabi- lity index	亲水性 GRAVY	信号肽 Signal peptide	亚细胞定位 Subcellular localization
Dlo000316	DLGDSL1	1 167	388	42.94	5.42	28.17	-0.12	有 Yes	叶绿体Chloroplast
Dlo001274	DLGDSL2	1 101	366	40.98	7.52	33.37	-0.16	有 Yes	内质网Endoplasmic reticulum
Dlo001275	DLGDSL3	1 101	366	40.82	6.93	32.28	-0.15	有 Yes	质膜Plasma membrane
Dlo003341	DLGDSL4	1 023	340	37.70	6.33	34.55	-0.13	无 No	细胞骨架Cytoskleton
Dlo003368	DLGDSL5	867	288	31.79	7.59	32.64	-0.12	无 No	细胞外基质Extracell
Dlo003369	DLGDSL6	1 083	360	38.37	8.00	28.46	0.18	有 Yes	细胞外基质Extracell
Dlo003726	DLGDSL7	1 092	363	40.07	9.20	27.33	-0.11	有 Yes	叶绿体/细胞外基质 Chloroplast/Extracell
Dlo003857	DLGDSL8	1 446	481	53.08	5.23	34.14	0.06	有 Yes	质膜Plasma membrane
Dlo004146	DLGDSL9	1 101	366	40.31	5.55	39.39	0.04	有 Yes	过氧物酶体Peroxisome
Dlo004148	DLGDSL10	1 104	367	40.39	8.43	29.70	-0.05	有 Yes	过氧物酶体Peroxisome
Dlo004149	DLGDSL11	1 113	370	40.58	8.17	35.43	-0.04	有 Yes	细胞外基质Extracell
Dlo004150	DLGDSL12	1 107	368	40.63	8.99	35.60	-0.01	有 Yes	细胞外基质Extracell
Dlo004345	DLGDSL13	1 116	371	40.72	5.62	37.99	-0.08	有 Yes	细胞外基质Extracell
Dlo004346	DLGDSL14	1 101	366	40.19	9.25	39.46	-0.04	有 Yes	细胞外基质Extracell
Dlo004347	DLGDSL15	1 110	369	40.53	8.78	30.98	-0.01	有 Yes	细胞外基质Extracell
Dlo004495	DLGDSL16	1 116	371	40.69	6.10	37.58	-0.07	有 Yes	细胞外基质Extracell
Dlo004496	DLGDSL17	999	332	36.12	9.05	36.33	0	有 Yes	细胞外基质Extracell
Dlo004497	DLGDSL18	999	332	36.12	9.05	36.33	0	有 Yes	细胞外基质Extracell
Dlo004498	DLGDSL19	1 074	357	39.06	8.45	31.23	-0.04	有 Yes	叶绿体Chloroplast
Dlo005884	DLGDSL20	1 164	387	42.84	8.56	32.73	0.01	有 Yes	细胞核Nucleus
Dlo006046	DLGDSL21	996	331	37.43	9.52	28.81	-0.27	无 No	叶绿体Chloroplast
Dlo006047	DLGDSL22	624	207	23.36	5.35	47.05	-0.58	无 No	细胞质Cytoplasm
Dlo006048	DLGDSL23	1 131	376	41.79	6.20	22.52	-0.14	有 Yes	叶绿体Chloroplast
Dlo006049	DLGDSL24	1 119	372	41.79	9.21	39.46	-0.35	无 No	细胞外基质Extracell
Dlo006050	DLGDSL25	1 686	561	62.33	9.24	31.14	-0.27	无 No	线粒体Mitochondrion
Dlo006051	DLGDSL26	1 098	365	40.35	9.34	25.33	-0.48	无 No	细胞质Cytoplasm
Dlo006445	DLGDSL27	1 146	381	41.12	8.94	27.42	0.02	有 Yes	细胞外基质Extracell
Dlo006446	DLGDSL28	1 002	333	35.79	9.15	21.43	0.06	有 Yes	细胞质Cytoplasm
Dlo007129	DLGDSL29	1 167	388	43.16	5.02	33.49	-0.08	有 Yes	细胞外基质Extracell
Dlo007130	DLGDSL30	1 167	388	43.32	4.69	33.41	-0.16	有 Yes	细胞外基质Extracell
Dlo007131	DLGDSL31	561	186	20.53	4.56	33.37	-0.07	有 Yes	叶绿体Chloroplast
Dlo007132	DLGDSL32	1 092	363	40.21	4.60	30.37	-0.11	有 Yes	细胞外基质Extracell
Dlo008436	DLGDSL33	1 050	349	39.54	4.82	27.58	-0.14	有 Yes	细胞质/细胞外基质 Cytoplasm/Extracell
Dlo008488	DLGDSL34	918	305	34.06	8.91	30.33	-0.12	有 Yes	叶绿体Chloroplast
Dlo008489	DLGDSL35	1 107	368	40.66	7.03	29.22	-0.11	有 Yes	细胞核/液泡 Nucleus/Vacuole
Dlo008490	DLGDSL36	1 005	334	37.49	7.55	27.31	-0.13	无 No	细胞质Cytoplasm
Dlo008545	DLGDSL37	1 101	366	40.92	8.56	34.17	-0.08	有 Yes	液泡Vacuole
Dlo008546	DLGDSL38	222	73	8.09	5.60	14.08	-0.46	无 No	细胞核Nucleus
Dlo011392	DLGDSL39	894	297	32.80	7.65	35.26	0.06	有 Yes	细胞外基质Extracell
Dlo011415	DLGDSL40	894	297	32.75	7.65	33.93	0.09	有 Yes	细胞外基质Extracell
Dlo011478	DLGDSL41	1 167	388	43.15	7.11	34.06	-0.17	有 Yes	细胞外基质Extracell
Dlo011955	DLGDSL42	1 311	436	48.64	9.01	37.95	-0.24	有 Yes	叶绿体/液泡 Chloroplast/Vacuole
基因ID Gene ID	基因名称 Gene name	开放阅读框/bp ORF	氨基酸数/aa Size	分子量/kD Molecular weight	等电点 pI	不稳定系数 The instabi- lity index	亲水性 GRAVY	信号肽 Signal peptide	亚细胞定位 Subcellular localization
Dlo011956	DLGDSL43	1 104	367	40.77	6.44	30.29	-0.09	有 Yes	细胞外基质Extracell
Dlo012251	DLGDSL44	1 353	450	50.16	5.44	36.36	-0.13	有 Yes	液泡Vacuole
Dlo012654	DLGDSL45	1 131	376	40.78	5.38	36.22	0.05	有 Yes	线粒体/液泡 Mitochondrion/Vacuole
Dlo013347	DLGDSL46	1 581	526	57.80	7.19	32.54	-0.06	有 Yes	内质网Endoplasmic reticulum
Dlo013348	DLGDSL47	759	252	27.24	5.80	26.12	0.10	有 Yes	叶绿体Chloroplast
Dlo013350	DLGDSL48	414	137	14.69	4.53	27.80	0.29	有 Yes	细胞质Cytoplasm
Dlo013351	DLGDSL49	633	210	23.36	6.74	35.80	-0.09	无 No	叶绿体Chloroplast
Dlo013352	DLGDSL50	462	153	16.18	8.61	36.41	0.26	有 Yes	细胞外基质Extracell
Dlo014916	DLGDSL51	1 116	371	40.33	8.89	24.93	-0.04	有 Yes	过氧物酶体Peroxisome
Dlo015050	DLGDSL52	1 092	363	39.38	5.09	22.52	-0.03	有 Yes	细胞质Cytoplasm
Dlo015051	DLGDSL53	1 047	348	38.79	9.18	30.01	-0.34	无 No	细胞外基质Extracell
Dlo015279	DLGDSL54	1 113	370	40.56	9.09	41.62	0.17	有 Yes	液泡Vacuole
Dlo015440	DLGDSL55	414	137	15.35	10.03	55.39	0.14	无 No	叶绿体Chloroplast
Dlo015441	DLGDSL56	897	298	33.14	5.96	46.73	0.08	有 Yes	液泡Vacuole
Dlo015778	DLGDSL57	1 119	372	40.88	8.78	22.47	-0.08	有 Yes	叶绿体/细胞核 Chloroplast/Nucleus
Dlo016597	DLGDSL58	1 044	347	37.54	5.06	34.60	-0.08	有 Yes	细胞外基质Extracell
Dlo018044	DLGDSL59	489	162	17.84	9.51	58.45	-0.19	无 No	叶绿体Chloroplast
Dlo018809	DLGDSL60	1 266	421	46.81	6.21	38.27	-0.28	有 Yes	线粒体Mitochondrion
Dlo018810	DLGDSL61	2 301	766	85.50	5.89	37.09	-0.40	无 No	线粒体Mitochondrion
Dlo018811	DLGDSL62	1 095	364	40.33	5.43	36.78	-0.07	有 Yes	叶绿体Chloroplast
Dlo018812	DLGDSL63	1 155	384	41.98	8.63	39.42	-0.12	有 Yes	细胞外基质Extracell
Dlo018813	DLGDSL64	1 002	333	37.13	9.22	31.41	-0.09	无 No	细胞质Cytoplasm
Dlo018821	DLGDSL65	1 155	384	42.61	8.78	34.10	-0.08	有 Yes	内质网Endoplasmic reticulum
Dlo018822	DLGDSL66	1 167	388	43.14	8.93	34.50	-0.05	有 Yes	叶绿体/细胞外基质 Chloroplast/Extracell
Dlo018823	DLGDSL67	1 155	384	41.99	8.63	39.92	-0.13	有 Yes	细胞外基质Extracell
Dlo018824	DLGDSL68	1 200	399	44.02	5.70	35.37	-0.09	有 Yes	细胞外基质Extracell
Dlo018825	DLGDSL69	1 236	411	45.68	6.07	41.05	-0.29	无 No	叶绿体/线粒体 Chloroplast/Mitochondrion
Dlo018826	DLGDSL70	1 182	393	43.94	7.60	35.32	-0.31	有 Yes	叶绿体Chloroplast
Dlo018827	DLGDSL71	711	236	26.64	7.03	37.54	-0.31	无 No	细胞外基质Extracell
Dlo018828	DLGDSL72	591	196	21.55	8.62	38.79	-0.23	有 Yes	细胞核/质膜 Nucleus/Plasma membrane
Dlo018829	DLGDSL73	1 185	394	43.98	5.42	34.99	-0.18	有 Yes	细胞外基质Extracell
Dlo018866	DLGDSL74	2 490	829	87.76	8.83	45.02	-0.72	有 Yes	细胞核Nucleus
Dlo018888	DLGDSL75	1 413	470	50.96	8.88	41.37	-0.26	有 Yes	细胞外基质Extracell
Dlo020895	DLGDSL76	1 071	356	39.13	7.53	28.29	-0.09	无 No	叶绿体/细胞外基质 Chloroplast/Extracell
Dlo020898	DLGDSL77	858	285	31.37	5.33	32.75	-0.19	无 No	叶绿体Chloroplast
Dlo021059	DLGDSL78	858	285	31.36	5.33	32.18	-0.19	无 No	叶绿体Chloroplast
Dlo021062	DLGDSL79	1 071	356	39.17	6.88	29.62	-0.11	有 Yes	细胞外基质Extracell
Dlo021694	DLGDSL80	1 101	366	40.98	5.69	22.79	-0.03	有 Yes	液泡Vacuole
Dlo021696	DLGDSL81	1 095	364	40.80	9.08	31.63	0.05	有 Yes	细胞核Nucleus
Dlo021719	DLGDSL82	1 140	379	41.98	8.65	42.73	-0.13	无 No	细胞质Cytoplasm
Dlo021829	DLGDSL83	2 457	818	91.37	8.14	46.45	-0.34	有 Yes	细胞外基质Extracell
基因ID Gene ID	基因名称 Gene name	开放阅读框/bp ORF	氨基酸数/aa Size	分子量/kD Molecular weight	等电点 pI	不稳定系数 The instabi- lity index	亲水性 GRAVY	信号肽 Signal peptide	亚细胞定位 Subcellular localization
Dlo021830	DLGDSL84	1 092	363	40.06	5.54	30.40	-0.27	有 Yes	细胞外基质/高尔基体 Extracell/Golgi apparatus
Dlo021839	DLGDSL85	1 101	366	40.78	9.30	32.43	-0.25	有 Yes	细胞外基质Extracell
Dlo022859	DLGDSL86	1 113	370	40.22	5.64	34.26	0.12	有 Yes	叶绿体/液泡 Chloroplast/vacu
Dlo023097	DLGDSL87	1 131	376	40.93	9.29	31.76	0.07	有 Yes	液泡Vacuole
Dlo024293	DLGDSL88	444	147	16.44	8.81	31.55	-0.09	无 No	细胞质Cytoplasm
Dlo024294	DLGDSL89	567	188	21.66	6.73	24.42	-0.33	无 No	细胞骨架Cytoskeleton
Dlo024299	DLGDSL90	465	154	17.31	7.69	20.21	-0.05	无 No	细胞质Cytoplasm
Dlo024338	DLGDSL91	1 284	427	47.21	8.25	38.18	-0.26	无 No	过氧物酶体Peroxisome
Dlo024344	DLGDSL92	972	323	36.11	6.55	31.35	-0.17	无 No	细胞质Cytoplasm
Dlo024345	DLGDSL93	972	323	36.11	6.55	31.35	-0.17	无 No	细胞质Cytoplasm
Dlo024482	DLGDSL94	1 023	340	37.62	8.80	37.81	-0.19	无 No	细胞外基质Extracell
Dlo025454	DLGDSL95	1 110	369	41.35	5.63	36.29	0.06	有 Yes	细胞质Cytoplasm
Dlo026075	DLGDSL96	1 062	353	38.56	8.47	33.85	0.06	有 Yes	细胞外基质Extracell
Dlo027030	DLGDSL97	1 092	363	40.93	8.65	33.01	-0.21	有 Yes	叶绿体/细胞质 Chloroplast/Cytoplasm
Dlo027031	DLGDSL98	1 068	355	39.56	8.01	31.74	-0.02	有 Yes	细胞外基质Extracell
Dlo027690	DLGDSL99	1 176	391	44.02	6.60	38.14	-0.33	有 Yes	细胞核/细胞质 Nucleus/Cytoplasm
Dlo027692	DLGDSL100	810	269	30.17	6.44	40.11	-0.19	有 Yes	细胞质Cytoplasm
Dlo027693	DLGDSL101	1 140	379	42.41	6.07	36.44	-0.27	有 Yes	内质网Endoplasmic reticulum
Dlo028167	DLGDSL102	1 041	346	38.63	5.39	27.19	-0.04	有 Yes	细胞质Cytoplasm
Dlo028168	DLGDSL103	960	319	35.64	5.22	34.19	-0.03	有 Yes	细胞外基质Extracell
Dlo029634	DLGDSL104	1 077	358	39.45	9.08	32.22	-0.11	有 Yes	叶绿体Chloroplast
Dlo030986	DLGDSL105	1 104	367	40.23	5.37	31.80	-0.14	有 Yes	细胞外基质Extracell
Dlo031180	DLGDSL106	810	269	28.88	5.12	27.46	0.14	有 Yes	细胞外基质Extracell
Dlo031921	DLGDSL107	1 170	389	43.18	8.78	36.17	-0.11	有 Yes	叶绿体Chloroplast
Dlo032044	DLGDSL108	804	267	29.89	7.09	50.80	-0.26	无 No	叶绿体Chloroplast
Dlo032046	DLGDSL109	798	265	29.70	5.63	47.67	-0.03	无 No	质膜Plasma membrane
Dlo032495	DLGDSL110	1 263	420	47.18	4.82	49.24	-0.06	有 Yes	液泡Vacuole
Dlo032524	DLGDSL111	1 149	382	42.75	6.01	33.02	-0.02	无 No	质膜Plasma membrane
Dlo033028	DLGDSL112	2 433	810	92.63	7.25	43.64	-0.36	无 No	叶绿体Chloroplast
Dlo033030	DLGDSL113	369	122	13.62	8.16	27.10	0.06	无 No	细胞质Cytoplasm
Dlo033228	DLGDSL114	1 146	381	41.12	8.94	27.42	0.02	有 Yes	细胞外基质Extracell
Dlo033229	DLGDSL115	1 092	363	38.87	8.97	20.51	-0.02	有 Yes	线粒体Mitochondrion
Dlo033340	DLGDSL116	1 113	370	40.19	5.41	34.06	0.12	有 Yes	液泡Vacuole
Dlo034402	DLGDSL117	756	251	27.63	9.24	34.38	-0.24	无 No	细胞核Nucleus
Dlo034801	DLGDSL118	1 122	373	41.26	7.45	31.18	-0.02	有 Yes	线粒体Mitochondrion

基因ID Gene ID	基因名称 Gene name	开放阅读框/bp ORF	氨基酸数/aa Size	分子量/kD Molecular weight	等电点 pI	不稳定系数 The instabi- lity index	亲水性 GRAVY	信号肽 Signal peptide	亚细胞定位 Subcellular localization
Dlo000316	DLGDSL1	1 167	388	42.94	5.42	28.17	-0.12	有 Yes	叶绿体Chloroplast
Dlo001274	DLGDSL2	1 101	366	40.98	7.52	33.37	-0.16	有 Yes	内质网Endoplasmic reticulum
Dlo001275	DLGDSL3	1 101	366	40.82	6.93	32.28	-0.15	有 Yes	质膜Plasma membrane
Dlo003341	DLGDSL4	1 023	340	37.70	6.33	34.55	-0.13	无 No	细胞骨架Cytoskleton
Dlo003368	DLGDSL5	867	288	31.79	7.59	32.64	-0.12	无 No	细胞外基质Extracell
Dlo003369	DLGDSL6	1 083	360	38.37	8.00	28.46	0.18	有 Yes	细胞外基质Extracell
Dlo003726	DLGDSL7	1 092	363	40.07	9.20	27.33	-0.11	有 Yes	叶绿体/细胞外基质 Chloroplast/Extracell
Dlo003857	DLGDSL8	1 446	481	53.08	5.23	34.14	0.06	有 Yes	质膜Plasma membrane
Dlo004146	DLGDSL9	1 101	366	40.31	5.55	39.39	0.04	有 Yes	过氧物酶体Peroxisome
Dlo004148	DLGDSL10	1 104	367	40.39	8.43	29.70	-0.05	有 Yes	过氧物酶体Peroxisome
Dlo004149	DLGDSL11	1 113	370	40.58	8.17	35.43	-0.04	有 Yes	细胞外基质Extracell
Dlo004150	DLGDSL12	1 107	368	40.63	8.99	35.60	-0.01	有 Yes	细胞外基质Extracell
Dlo004345	DLGDSL13	1 116	371	40.72	5.62	37.99	-0.08	有 Yes	细胞外基质Extracell
Dlo004346	DLGDSL14	1 101	366	40.19	9.25	39.46	-0.04	有 Yes	细胞外基质Extracell
Dlo004347	DLGDSL15	1 110	369	40.53	8.78	30.98	-0.01	有 Yes	细胞外基质Extracell
Dlo004495	DLGDSL16	1 116	371	40.69	6.10	37.58	-0.07	有 Yes	细胞外基质Extracell
Dlo004496	DLGDSL17	999	332	36.12	9.05	36.33	0	有 Yes	细胞外基质Extracell
Dlo004497	DLGDSL18	999	332	36.12	9.05	36.33	0	有 Yes	细胞外基质Extracell
Dlo004498	DLGDSL19	1 074	357	39.06	8.45	31.23	-0.04	有 Yes	叶绿体Chloroplast
Dlo005884	DLGDSL20	1 164	387	42.84	8.56	32.73	0.01	有 Yes	细胞核Nucleus
Dlo006046	DLGDSL21	996	331	37.43	9.52	28.81	-0.27	无 No	叶绿体Chloroplast
Dlo006047	DLGDSL22	624	207	23.36	5.35	47.05	-0.58	无 No	细胞质Cytoplasm
Dlo006048	DLGDSL23	1 131	376	41.79	6.20	22.52	-0.14	有 Yes	叶绿体Chloroplast
Dlo006049	DLGDSL24	1 119	372	41.79	9.21	39.46	-0.35	无 No	细胞外基质Extracell
Dlo006050	DLGDSL25	1 686	561	62.33	9.24	31.14	-0.27	无 No	线粒体Mitochondrion
Dlo006051	DLGDSL26	1 098	365	40.35	9.34	25.33	-0.48	无 No	细胞质Cytoplasm
Dlo006445	DLGDSL27	1 146	381	41.12	8.94	27.42	0.02	有 Yes	细胞外基质Extracell
Dlo006446	DLGDSL28	1 002	333	35.79	9.15	21.43	0.06	有 Yes	细胞质Cytoplasm
Dlo007129	DLGDSL29	1 167	388	43.16	5.02	33.49	-0.08	有 Yes	细胞外基质Extracell
Dlo007130	DLGDSL30	1 167	388	43.32	4.69	33.41	-0.16	有 Yes	细胞外基质Extracell
Dlo007131	DLGDSL31	561	186	20.53	4.56	33.37	-0.07	有 Yes	叶绿体Chloroplast
Dlo007132	DLGDSL32	1 092	363	40.21	4.60	30.37	-0.11	有 Yes	细胞外基质Extracell
Dlo008436	DLGDSL33	1 050	349	39.54	4.82	27.58	-0.14	有 Yes	细胞质/细胞外基质 Cytoplasm/Extracell
Dlo008488	DLGDSL34	918	305	34.06	8.91	30.33	-0.12	有 Yes	叶绿体Chloroplast
Dlo008489	DLGDSL35	1 107	368	40.66	7.03	29.22	-0.11	有 Yes	细胞核/液泡 Nucleus/Vacuole
Dlo008490	DLGDSL36	1 005	334	37.49	7.55	27.31	-0.13	无 No	细胞质Cytoplasm
Dlo008545	DLGDSL37	1 101	366	40.92	8.56	34.17	-0.08	有 Yes	液泡Vacuole
Dlo008546	DLGDSL38	222	73	8.09	5.60	14.08	-0.46	无 No	细胞核Nucleus
Dlo011392	DLGDSL39	894	297	32.80	7.65	35.26	0.06	有 Yes	细胞外基质Extracell
Dlo011415	DLGDSL40	894	297	32.75	7.65	33.93	0.09	有 Yes	细胞外基质Extracell
Dlo011478	DLGDSL41	1 167	388	43.15	7.11	34.06	-0.17	有 Yes	细胞外基质Extracell
Dlo011955	DLGDSL42	1 311	436	48.64	9.01	37.95	-0.24	有 Yes	叶绿体/液泡 Chloroplast/Vacuole
基因ID Gene ID	基因名称 Gene name	开放阅读框/bp ORF	氨基酸数/aa Size	分子量/kD Molecular weight	等电点 pI	不稳定系数 The instabi- lity index	亲水性 GRAVY	信号肽 Signal peptide	亚细胞定位 Subcellular localization
Dlo011956	DLGDSL43	1 104	367	40.77	6.44	30.29	-0.09	有 Yes	细胞外基质Extracell
Dlo012251	DLGDSL44	1 353	450	50.16	5.44	36.36	-0.13	有 Yes	液泡Vacuole
Dlo012654	DLGDSL45	1 131	376	40.78	5.38	36.22	0.05	有 Yes	线粒体/液泡 Mitochondrion/Vacuole
Dlo013347	DLGDSL46	1 581	526	57.80	7.19	32.54	-0.06	有 Yes	内质网Endoplasmic reticulum
Dlo013348	DLGDSL47	759	252	27.24	5.80	26.12	0.10	有 Yes	叶绿体Chloroplast
Dlo013350	DLGDSL48	414	137	14.69	4.53	27.80	0.29	有 Yes	细胞质Cytoplasm
Dlo013351	DLGDSL49	633	210	23.36	6.74	35.80	-0.09	无 No	叶绿体Chloroplast
Dlo013352	DLGDSL50	462	153	16.18	8.61	36.41	0.26	有 Yes	细胞外基质Extracell
Dlo014916	DLGDSL51	1 116	371	40.33	8.89	24.93	-0.04	有 Yes	过氧物酶体Peroxisome
Dlo015050	DLGDSL52	1 092	363	39.38	5.09	22.52	-0.03	有 Yes	细胞质Cytoplasm
Dlo015051	DLGDSL53	1 047	348	38.79	9.18	30.01	-0.34	无 No	细胞外基质Extracell
Dlo015279	DLGDSL54	1 113	370	40.56	9.09	41.62	0.17	有 Yes	液泡Vacuole
Dlo015440	DLGDSL55	414	137	15.35	10.03	55.39	0.14	无 No	叶绿体Chloroplast
Dlo015441	DLGDSL56	897	298	33.14	5.96	46.73	0.08	有 Yes	液泡Vacuole
Dlo015778	DLGDSL57	1 119	372	40.88	8.78	22.47	-0.08	有 Yes	叶绿体/细胞核 Chloroplast/Nucleus
Dlo016597	DLGDSL58	1 044	347	37.54	5.06	34.60	-0.08	有 Yes	细胞外基质Extracell
Dlo018044	DLGDSL59	489	162	17.84	9.51	58.45	-0.19	无 No	叶绿体Chloroplast
Dlo018809	DLGDSL60	1 266	421	46.81	6.21	38.27	-0.28	有 Yes	线粒体Mitochondrion
Dlo018810	DLGDSL61	2 301	766	85.50	5.89	37.09	-0.40	无 No	线粒体Mitochondrion
Dlo018811	DLGDSL62	1 095	364	40.33	5.43	36.78	-0.07	有 Yes	叶绿体Chloroplast
Dlo018812	DLGDSL63	1 155	384	41.98	8.63	39.42	-0.12	有 Yes	细胞外基质Extracell
Dlo018813	DLGDSL64	1 002	333	37.13	9.22	31.41	-0.09	无 No	细胞质Cytoplasm
Dlo018821	DLGDSL65	1 155	384	42.61	8.78	34.10	-0.08	有 Yes	内质网Endoplasmic reticulum
Dlo018822	DLGDSL66	1 167	388	43.14	8.93	34.50	-0.05	有 Yes	叶绿体/细胞外基质 Chloroplast/Extracell
Dlo018823	DLGDSL67	1 155	384	41.99	8.63	39.92	-0.13	有 Yes	细胞外基质Extracell
Dlo018824	DLGDSL68	1 200	399	44.02	5.70	35.37	-0.09	有 Yes	细胞外基质Extracell
Dlo018825	DLGDSL69	1 236	411	45.68	6.07	41.05	-0.29	无 No	叶绿体/线粒体 Chloroplast/Mitochondrion
Dlo018826	DLGDSL70	1 182	393	43.94	7.60	35.32	-0.31	有 Yes	叶绿体Chloroplast
Dlo018827	DLGDSL71	711	236	26.64	7.03	37.54	-0.31	无 No	细胞外基质Extracell
Dlo018828	DLGDSL72	591	196	21.55	8.62	38.79	-0.23	有 Yes	细胞核/质膜 Nucleus/Plasma membrane
Dlo018829	DLGDSL73	1 185	394	43.98	5.42	34.99	-0.18	有 Yes	细胞外基质Extracell
Dlo018866	DLGDSL74	2 490	829	87.76	8.83	45.02	-0.72	有 Yes	细胞核Nucleus
Dlo018888	DLGDSL75	1 413	470	50.96	8.88	41.37	-0.26	有 Yes	细胞外基质Extracell
Dlo020895	DLGDSL76	1 071	356	39.13	7.53	28.29	-0.09	无 No	叶绿体/细胞外基质 Chloroplast/Extracell
Dlo020898	DLGDSL77	858	285	31.37	5.33	32.75	-0.19	无 No	叶绿体Chloroplast
Dlo021059	DLGDSL78	858	285	31.36	5.33	32.18	-0.19	无 No	叶绿体Chloroplast
Dlo021062	DLGDSL79	1 071	356	39.17	6.88	29.62	-0.11	有 Yes	细胞外基质Extracell
Dlo021694	DLGDSL80	1 101	366	40.98	5.69	22.79	-0.03	有 Yes	液泡Vacuole
Dlo021696	DLGDSL81	1 095	364	40.80	9.08	31.63	0.05	有 Yes	细胞核Nucleus
Dlo021719	DLGDSL82	1 140	379	41.98	8.65	42.73	-0.13	无 No	细胞质Cytoplasm
Dlo021829	DLGDSL83	2 457	818	91.37	8.14	46.45	-0.34	有 Yes	细胞外基质Extracell
基因ID Gene ID	基因名称 Gene name	开放阅读框/bp ORF	氨基酸数/aa Size	分子量/kD Molecular weight	等电点 pI	不稳定系数 The instabi- lity index	亲水性 GRAVY	信号肽 Signal peptide	亚细胞定位 Subcellular localization
Dlo021830	DLGDSL84	1 092	363	40.06	5.54	30.40	-0.27	有 Yes	细胞外基质/高尔基体 Extracell/Golgi apparatus
Dlo021839	DLGDSL85	1 101	366	40.78	9.30	32.43	-0.25	有 Yes	细胞外基质Extracell
Dlo022859	DLGDSL86	1 113	370	40.22	5.64	34.26	0.12	有 Yes	叶绿体/液泡 Chloroplast/vacu
Dlo023097	DLGDSL87	1 131	376	40.93	9.29	31.76	0.07	有 Yes	液泡Vacuole
Dlo024293	DLGDSL88	444	147	16.44	8.81	31.55	-0.09	无 No	细胞质Cytoplasm
Dlo024294	DLGDSL89	567	188	21.66	6.73	24.42	-0.33	无 No	细胞骨架Cytoskeleton
Dlo024299	DLGDSL90	465	154	17.31	7.69	20.21	-0.05	无 No	细胞质Cytoplasm
Dlo024338	DLGDSL91	1 284	427	47.21	8.25	38.18	-0.26	无 No	过氧物酶体Peroxisome
Dlo024344	DLGDSL92	972	323	36.11	6.55	31.35	-0.17	无 No	细胞质Cytoplasm
Dlo024345	DLGDSL93	972	323	36.11	6.55	31.35	-0.17	无 No	细胞质Cytoplasm
Dlo024482	DLGDSL94	1 023	340	37.62	8.80	37.81	-0.19	无 No	细胞外基质Extracell
Dlo025454	DLGDSL95	1 110	369	41.35	5.63	36.29	0.06	有 Yes	细胞质Cytoplasm
Dlo026075	DLGDSL96	1 062	353	38.56	8.47	33.85	0.06	有 Yes	细胞外基质Extracell
Dlo027030	DLGDSL97	1 092	363	40.93	8.65	33.01	-0.21	有 Yes	叶绿体/细胞质 Chloroplast/Cytoplasm
Dlo027031	DLGDSL98	1 068	355	39.56	8.01	31.74	-0.02	有 Yes	细胞外基质Extracell
Dlo027690	DLGDSL99	1 176	391	44.02	6.60	38.14	-0.33	有 Yes	细胞核/细胞质 Nucleus/Cytoplasm
Dlo027692	DLGDSL100	810	269	30.17	6.44	40.11	-0.19	有 Yes	细胞质Cytoplasm
Dlo027693	DLGDSL101	1 140	379	42.41	6.07	36.44	-0.27	有 Yes	内质网Endoplasmic reticulum
Dlo028167	DLGDSL102	1 041	346	38.63	5.39	27.19	-0.04	有 Yes	细胞质Cytoplasm
Dlo028168	DLGDSL103	960	319	35.64	5.22	34.19	-0.03	有 Yes	细胞外基质Extracell
Dlo029634	DLGDSL104	1 077	358	39.45	9.08	32.22	-0.11	有 Yes	叶绿体Chloroplast
Dlo030986	DLGDSL105	1 104	367	40.23	5.37	31.80	-0.14	有 Yes	细胞外基质Extracell
Dlo031180	DLGDSL106	810	269	28.88	5.12	27.46	0.14	有 Yes	细胞外基质Extracell
Dlo031921	DLGDSL107	1 170	389	43.18	8.78	36.17	-0.11	有 Yes	叶绿体Chloroplast
Dlo032044	DLGDSL108	804	267	29.89	7.09	50.80	-0.26	无 No	叶绿体Chloroplast
Dlo032046	DLGDSL109	798	265	29.70	5.63	47.67	-0.03	无 No	质膜Plasma membrane
Dlo032495	DLGDSL110	1 263	420	47.18	4.82	49.24	-0.06	有 Yes	液泡Vacuole
Dlo032524	DLGDSL111	1 149	382	42.75	6.01	33.02	-0.02	无 No	质膜Plasma membrane
Dlo033028	DLGDSL112	2 433	810	92.63	7.25	43.64	-0.36	无 No	叶绿体Chloroplast
Dlo033030	DLGDSL113	369	122	13.62	8.16	27.10	0.06	无 No	细胞质Cytoplasm
Dlo033228	DLGDSL114	1 146	381	41.12	8.94	27.42	0.02	有 Yes	细胞外基质Extracell
Dlo033229	DLGDSL115	1 092	363	38.87	8.97	20.51	-0.02	有 Yes	线粒体Mitochondrion
Dlo033340	DLGDSL116	1 113	370	40.19	5.41	34.06	0.12	有 Yes	液泡Vacuole
Dlo034402	DLGDSL117	756	251	27.63	9.24	34.38	-0.24	无 No	细胞核Nucleus
Dlo034801	DLGDSL118	1 122	373	41.26	7.45	31.18	-0.02	有 Yes	线粒体Mitochondrion