园艺学报 ›› 2022, Vol. 49 ›› Issue (2): 252-264.doi: 10.16420/j.issn.0513-353x.2020-0724
收稿日期:
2021-05-28
修回日期:
2021-09-30
出版日期:
2022-02-25
发布日期:
2022-02-28
通讯作者:
叶新福
E-mail:yexinfu@126.com
基金资助:
JIANG Cuicui, FANG Zhizhen, ZHOU Danrong, LIN Yanjuan, YE Xinfu()
Received:
2021-05-28
Revised:
2021-09-30
Online:
2022-02-25
Published:
2022-02-28
Contact:
YE Xinfu
E-mail:yexinfu@126.com
摘要:
基于‘芙蓉李’(Prunus salicina)基因组数据,采用生物信息学分析方法鉴定糖转运蛋白家族基因,并对其蛋白质理化特性、亚细胞定位、系统进化树、蛋白质保守结构域以及表达模式进行分析。共鉴定出49个该家族基因,根据保守结构域和系统发育分析将其分为7个亚家族。蛋白质介于313 ~ 948个氨基酸之间,预测定位于质膜或液泡膜;所有的蛋白都含有保守的糖转运体结构域(PF00083),其中大多数含有12个跨膜螺旋。通过比对‘芙蓉李’果实发育过程的RNA-seq数据,对49个基因进行表达谱分析,其中15个在果实不同发育阶段差异表达;qRT-PCR分析表明,15个差异表达基因中有9个基因与果实葡萄糖和蔗糖含量显著正/负相关,其中1个肌醇转运蛋白亚家族基因(PsINT4)、1个糖转运蛋白亚家族基因(PsSTP11)、1个糖促进蛋白亚家族基因(PsSFP5)和1个液泡单糖转运蛋白亚家族基因(PsTMT1)在‘芙蓉李’果实发育过程中具有较高的表达量,可能在‘芙蓉李’果实发育和成熟过程中对糖分积累起重要作用。
中图分类号:
姜翠翠, 方智振, 周丹蓉, 林炎娟, 叶新福. ‘芙蓉李’糖转运蛋白家族基因鉴定及表达分析[J]. 园艺学报, 2022, 49(2): 252-264.
JIANG Cuicui, FANG Zhizhen, ZHOU Danrong, LIN Yanjuan, YE Xinfu. Identification and Expression Analysis of Sugar Transporter Family Genes in‘Furongli’(Prunus salicina)[J]. Acta Horticulturae Sinica, 2022, 49(2): 252-264.
序列编号 Gene ID | 基因名称 Gene name | 正向引物(5′-3′) Forward primer | 反向引物(5′-3′) Reverse primer |
---|---|---|---|
EVM0028193.1 | PsSUC1 | AAGTCGCCATCGCAGAAGG | AGAGCCAGACGAAAGCGGA |
EVM0017205.2 | PsTMT1 | GGTATTGTAATGCTGTGGGCTC | CCTTATTTCAGGTGGTGCTGTC |
EVM0013841.1 | PsPMT6 | CAACCCCAGAAAACCATAGCAGA | CCGAGCCGATGAGGGAGTAAAG |
EVM0025082.1 | PsINT2 | CCAGATTGCGGGTTTTGTGC | AAAGTGCCAAGCCAGTTAGTGC |
EVM0027680.1 | PsINT4 | TTACAGCCCAACAATCGTTCAGT | GCCTTCTCCTCCCATATCTATCCA |
EVM0013528.1 | PsSFP4 | TGGCAACTGGATACGGAATGG | ATGGTCACTGCACAGGGAACG |
EVM0002384.1 | PsSFP5 | ATGGTCACTGCACAGGGAACG | GCTGACATTCTCATTGCCCCTTT |
EVM0021731.1 | PsSTP11 | AAGCAGGGCTCCTTGTCCATA | CCAGAGCAGCAAGATACAGCA |
EVM0014989.1 | PsSTP17 | CTTGCTTGGTGTTGGGGTTGG | GGTGGTCGTTGGTGCTTTGGATA |
表1 实时荧光定量引物序列
Table 1 Primer pairs used in qRT-PCR analysis
序列编号 Gene ID | 基因名称 Gene name | 正向引物(5′-3′) Forward primer | 反向引物(5′-3′) Reverse primer |
---|---|---|---|
EVM0028193.1 | PsSUC1 | AAGTCGCCATCGCAGAAGG | AGAGCCAGACGAAAGCGGA |
EVM0017205.2 | PsTMT1 | GGTATTGTAATGCTGTGGGCTC | CCTTATTTCAGGTGGTGCTGTC |
EVM0013841.1 | PsPMT6 | CAACCCCAGAAAACCATAGCAGA | CCGAGCCGATGAGGGAGTAAAG |
EVM0025082.1 | PsINT2 | CCAGATTGCGGGTTTTGTGC | AAAGTGCCAAGCCAGTTAGTGC |
EVM0027680.1 | PsINT4 | TTACAGCCCAACAATCGTTCAGT | GCCTTCTCCTCCCATATCTATCCA |
EVM0013528.1 | PsSFP4 | TGGCAACTGGATACGGAATGG | ATGGTCACTGCACAGGGAACG |
EVM0002384.1 | PsSFP5 | ATGGTCACTGCACAGGGAACG | GCTGACATTCTCATTGCCCCTTT |
EVM0021731.1 | PsSTP11 | AAGCAGGGCTCCTTGTCCATA | CCAGAGCAGCAAGATACAGCA |
EVM0014989.1 | PsSTP17 | CTTGCTTGGTGTTGGGGTTGG | GGTGGTCGTTGGTGCTTTGGATA |
序列编号 Gene ID | 基因名称 Gene name | CDS/bp | 蛋白质/aa Protein | 分子量/kD Molecular weigh | 等电点 pI | 亚细胞定位 Cell location | E值 E-value |
---|---|---|---|---|---|---|---|
EVM0028115.1 | PsSTP1 | 1 533 | 511 | 55.32 | 9.53 | 液泡膜 Vacuolar membrane | 7.10E-111 |
EVM0005102.1 | PsSTP2 | 1 551 | 517 | 56.19 | 9.61 | 液泡膜 Vacuolar membrane | 4.00E-119 |
EVM0027704.1 | PsSTP3 | 1 596 | 532 | 58.07 | 9.16 | 质膜 Plasma membrane | 5.80E-127 |
EVM0008700.1 | PsSTP4 | 1 542 | 514 | 55.97 | 8.70 | 质膜 Plasma membrane | 8.60E-128 |
EVM0000375.1 | PsSTP5 | 1 584 | 528 | 57.87 | 9.24 | 液泡膜 Vacuolar membrane | 1.50E-122 |
EVM0026713.1 | PsSTP6 | 1 533 | 511 | 55.91 | 9.42 | 液泡膜 Vacuolar membrane | 2.50E-124 |
EVM0020907.1 | PsSTP7 | 1 533 | 511 | 56.06 | 9.55 | 液泡膜 Vacuolar membrane | 1.50E-122 |
EVM0019545.1 | PsSTP8 | 1 404 | 468 | 52.71 | 9.76 | 液泡膜 Vacuolar membrane | 5.70E-87 |
EVM0015455.1 | PsSTP9 | 1 503 | 510 | 56.54 | 8.30 | 质膜 Plasma membrane | 4.60E-125 |
EVM0003755.1 | PsSTP10 | 1 545 | 515 | 56.77 | 10.07 | 质膜 Plasma membrane | 5.90E-118 |
EVM0021731.1 | PsSTP11 | 1 509 | 503 | 55.18 | 9.84 | 质膜 Plasma membrane | 1.00E-117 |
EVM0025512.1 | PsSTP12 | 1 545 | 515 | 55.98 | 9.25 | 质膜 Plasma membrane | 4.30E-119 |
EVM0006054.1 | PsSTP13 | 1 566 | 522 | 57.67 | 9.14 | 质膜 Plasma membrane | 6.40E-136 |
EVM0000188.1 | PsSTP14 | 1 551 | 517 | 55.53 | 9.67 | 质膜 Plasma membrane | 1.20E-124 |
EVM0006545.1 | PsSTP15 | 1 551 | 517 | 55.66 | 9.65 | 质膜 Plasma membrane | 1.70E-125 |
EVM0015287.1 | PsSTP16 | 1 524 | 508 | 55.39 | 9.88 | 质膜 Plasma membrane | 1.30E-118 |
EVM0014989.1 | PsSTP17 | 1 317 | 439 | 47.73 | 10.04 | 质膜 Plasma membrane | 2.80E-77 |
EVM0006896.1 | PsSTP18 | 1 521 | 507 | 55.23 | 8.93 | 液泡膜 Vacuolar membrane | 9.80E-114 |
EVM0025706.1 | PsSFP1 | 1 467 | 489 | 52.65 | 8.38 | 质膜 Plasma membrane | 7.60E-84 |
EVM0002206.1 | PsSFP2 | 1 458 | 486 | 52.44 | 9.00 | 质膜 Plasma membrane | 1.30E-98 |
EVM0026716.1 | PsSFP3 | 939 | 313 | 34.10 | 8.57 | 质膜 Plasma membrane | 2.30E-46 |
EVM0013528.1 | PsSFP4 | 2 844 | 948 | 102.12 | 8.59 | 质膜 Plasma membrane | 6.30E-169 |
EVM0002384.1 | PsSFP5 | 1 482 | 494 | 53.81 | 6.65 | 质膜 Plasma membrane | 1.70E-70 |
EVM0019887.1 | PspGlcT1 | 1 611 | 537 | 58.56 | 8.20 | 质膜 Plasma membrane | 1.00E-99 |
EVM0017959.1 | PspGlcT2 | 1 500 | 500 | 54.02 | 8.54 | 质膜 Plasma membrane | 1.30E-94 |
EVM0012278.1 | PspGlcT3 | 1 368 | 456 | 48.44 | 7.68 | 液泡膜 Vacuolar membrane | 3.00E-58 |
EVM0010156.1 | PspGlcT4 | 1 647 | 549 | 57.35 | 9.62 | 质膜 Plasma membrane | 6.10E-106 |
EVM0002496.1 | PsINT1 | 1 494 | 498 | 53.86 | 4.79 | 质膜 Plasma membrane | 5.10E-123 |
EVM0025082.1 | PsINT2 | 1 731 | 577 | 62.94 | 8.80 | 质膜 Plasma membrane | 4.10E-123 |
EVM0018913.1 | PsINT3 | 1 734 | 578 | 62.65 | 9.00 | 质膜 Plasma membrane | 1.30E-119 |
EVM0027680.1 | PsINT4 | 1 728 | 576 | 62.02 | 8.62 | 质膜 Plasma membrane | 2.40E-116 |
EVM0024467.1 | PsPMT1 | 1 584 | 528 | 57.42 | 8.76 | 质膜 Plasma membrane | 6.10E-106 |
EVM0006809.1 | PsPMT2 | 1 575 | 525 | 56.92 | 9.70 | 质膜 Plasma membrane | 4.80E-106 |
EVM0013455.1 | PsPMT3 | 1 524 | 508 | 54.47 | 5.98 | 质膜 Plasma membrane | 6.70E-104 |
EVM0010693.1 | PsPMT4 | 1 587 | 529 | 56.73 | 5.07 | 液泡膜 Vacuolar membrane | 7.10E-110 |
EVM0014524.1 | PsPMT5 | 1 473 | 491 | 53.14 | 9.63 | 质膜 Plasma membrane | 9.80E-93 |
EVM0013841.1 | PsPMT6 | 1 614 | 538 | 58.37 | 9.44 | 质膜 Plasma membrane | 2.80E-108 |
EVM0016266.1 | PsPMT7 | 1 593 | 531 | 57.74 | 8.28 | 质膜 Plasma membrane | 4.50E-104 |
EVM0014991.1 | PsPMT8 | 1 533 | 511 | 55.69 | 8.96 | 质膜 Plasma membrane | 5.40E-105 |
EVM0010139.1 | PsPMT9 | 1 527 | 509 | 55.44 | 8.34 | 质膜 Plasma membrane | 9.40E-104 |
EVM0020369.1 | PsPMT10 | 1 641 | 547 | 58.86 | 7.28 | 质膜 Plasma membrane | 6.10E-107 |
EVM0007278.1 | PsPMT11 | 1 497 | 499 | 54.02 | 9.59 | 液泡膜 Vacuolar membrane | 9.70E-109 |
EVM0017205.2 | PsTMT1 | 2 208 | 736 | 78.40 | 5.24 | 质膜 Plasma membrane | 1.80E-92 |
EVM0000294.1 | PsTMT2 | 2 226 | 742 | 79.99 | 5.09 | 质膜 Plasma membrane | 2.70E-96 |
EVM0025033.1 | PsTMT3 | 2 178 | 726 | 77.92 | 4.97 | 质膜 Plasma membrane | 4.80E-84 |
EVM0024253.1 | PsTMT4 | 2 217 | 739 | 79.07 | 4.56 | 质膜 Plasma membrane | 1.10E-90 |
EVM0028193.1 | PsSUC1 | 1 533 | 511 | 53.90 | 9.07 | 质膜 Plasma membrane | 3.10E-80 |
EVM0013799.1 | PsSUC2 | 1 827 | 609 | 65.44 | 7.03 | 质膜 Plasma membrane | 4.20E-94 |
EVM0001814.2 | PsSUC3 | 1 497 | 499 | 53.68 | 9.21 | 质膜 Plasma membrane | 5.10E-92 |
表2 ‘芙蓉李’糖转运蛋白家族基因的结构及生物学信息
Table 2 Structural and biochemical information of sugar transporter family members in‘Furongli’plum
序列编号 Gene ID | 基因名称 Gene name | CDS/bp | 蛋白质/aa Protein | 分子量/kD Molecular weigh | 等电点 pI | 亚细胞定位 Cell location | E值 E-value |
---|---|---|---|---|---|---|---|
EVM0028115.1 | PsSTP1 | 1 533 | 511 | 55.32 | 9.53 | 液泡膜 Vacuolar membrane | 7.10E-111 |
EVM0005102.1 | PsSTP2 | 1 551 | 517 | 56.19 | 9.61 | 液泡膜 Vacuolar membrane | 4.00E-119 |
EVM0027704.1 | PsSTP3 | 1 596 | 532 | 58.07 | 9.16 | 质膜 Plasma membrane | 5.80E-127 |
EVM0008700.1 | PsSTP4 | 1 542 | 514 | 55.97 | 8.70 | 质膜 Plasma membrane | 8.60E-128 |
EVM0000375.1 | PsSTP5 | 1 584 | 528 | 57.87 | 9.24 | 液泡膜 Vacuolar membrane | 1.50E-122 |
EVM0026713.1 | PsSTP6 | 1 533 | 511 | 55.91 | 9.42 | 液泡膜 Vacuolar membrane | 2.50E-124 |
EVM0020907.1 | PsSTP7 | 1 533 | 511 | 56.06 | 9.55 | 液泡膜 Vacuolar membrane | 1.50E-122 |
EVM0019545.1 | PsSTP8 | 1 404 | 468 | 52.71 | 9.76 | 液泡膜 Vacuolar membrane | 5.70E-87 |
EVM0015455.1 | PsSTP9 | 1 503 | 510 | 56.54 | 8.30 | 质膜 Plasma membrane | 4.60E-125 |
EVM0003755.1 | PsSTP10 | 1 545 | 515 | 56.77 | 10.07 | 质膜 Plasma membrane | 5.90E-118 |
EVM0021731.1 | PsSTP11 | 1 509 | 503 | 55.18 | 9.84 | 质膜 Plasma membrane | 1.00E-117 |
EVM0025512.1 | PsSTP12 | 1 545 | 515 | 55.98 | 9.25 | 质膜 Plasma membrane | 4.30E-119 |
EVM0006054.1 | PsSTP13 | 1 566 | 522 | 57.67 | 9.14 | 质膜 Plasma membrane | 6.40E-136 |
EVM0000188.1 | PsSTP14 | 1 551 | 517 | 55.53 | 9.67 | 质膜 Plasma membrane | 1.20E-124 |
EVM0006545.1 | PsSTP15 | 1 551 | 517 | 55.66 | 9.65 | 质膜 Plasma membrane | 1.70E-125 |
EVM0015287.1 | PsSTP16 | 1 524 | 508 | 55.39 | 9.88 | 质膜 Plasma membrane | 1.30E-118 |
EVM0014989.1 | PsSTP17 | 1 317 | 439 | 47.73 | 10.04 | 质膜 Plasma membrane | 2.80E-77 |
EVM0006896.1 | PsSTP18 | 1 521 | 507 | 55.23 | 8.93 | 液泡膜 Vacuolar membrane | 9.80E-114 |
EVM0025706.1 | PsSFP1 | 1 467 | 489 | 52.65 | 8.38 | 质膜 Plasma membrane | 7.60E-84 |
EVM0002206.1 | PsSFP2 | 1 458 | 486 | 52.44 | 9.00 | 质膜 Plasma membrane | 1.30E-98 |
EVM0026716.1 | PsSFP3 | 939 | 313 | 34.10 | 8.57 | 质膜 Plasma membrane | 2.30E-46 |
EVM0013528.1 | PsSFP4 | 2 844 | 948 | 102.12 | 8.59 | 质膜 Plasma membrane | 6.30E-169 |
EVM0002384.1 | PsSFP5 | 1 482 | 494 | 53.81 | 6.65 | 质膜 Plasma membrane | 1.70E-70 |
EVM0019887.1 | PspGlcT1 | 1 611 | 537 | 58.56 | 8.20 | 质膜 Plasma membrane | 1.00E-99 |
EVM0017959.1 | PspGlcT2 | 1 500 | 500 | 54.02 | 8.54 | 质膜 Plasma membrane | 1.30E-94 |
EVM0012278.1 | PspGlcT3 | 1 368 | 456 | 48.44 | 7.68 | 液泡膜 Vacuolar membrane | 3.00E-58 |
EVM0010156.1 | PspGlcT4 | 1 647 | 549 | 57.35 | 9.62 | 质膜 Plasma membrane | 6.10E-106 |
EVM0002496.1 | PsINT1 | 1 494 | 498 | 53.86 | 4.79 | 质膜 Plasma membrane | 5.10E-123 |
EVM0025082.1 | PsINT2 | 1 731 | 577 | 62.94 | 8.80 | 质膜 Plasma membrane | 4.10E-123 |
EVM0018913.1 | PsINT3 | 1 734 | 578 | 62.65 | 9.00 | 质膜 Plasma membrane | 1.30E-119 |
EVM0027680.1 | PsINT4 | 1 728 | 576 | 62.02 | 8.62 | 质膜 Plasma membrane | 2.40E-116 |
EVM0024467.1 | PsPMT1 | 1 584 | 528 | 57.42 | 8.76 | 质膜 Plasma membrane | 6.10E-106 |
EVM0006809.1 | PsPMT2 | 1 575 | 525 | 56.92 | 9.70 | 质膜 Plasma membrane | 4.80E-106 |
EVM0013455.1 | PsPMT3 | 1 524 | 508 | 54.47 | 5.98 | 质膜 Plasma membrane | 6.70E-104 |
EVM0010693.1 | PsPMT4 | 1 587 | 529 | 56.73 | 5.07 | 液泡膜 Vacuolar membrane | 7.10E-110 |
EVM0014524.1 | PsPMT5 | 1 473 | 491 | 53.14 | 9.63 | 质膜 Plasma membrane | 9.80E-93 |
EVM0013841.1 | PsPMT6 | 1 614 | 538 | 58.37 | 9.44 | 质膜 Plasma membrane | 2.80E-108 |
EVM0016266.1 | PsPMT7 | 1 593 | 531 | 57.74 | 8.28 | 质膜 Plasma membrane | 4.50E-104 |
EVM0014991.1 | PsPMT8 | 1 533 | 511 | 55.69 | 8.96 | 质膜 Plasma membrane | 5.40E-105 |
EVM0010139.1 | PsPMT9 | 1 527 | 509 | 55.44 | 8.34 | 质膜 Plasma membrane | 9.40E-104 |
EVM0020369.1 | PsPMT10 | 1 641 | 547 | 58.86 | 7.28 | 质膜 Plasma membrane | 6.10E-107 |
EVM0007278.1 | PsPMT11 | 1 497 | 499 | 54.02 | 9.59 | 液泡膜 Vacuolar membrane | 9.70E-109 |
EVM0017205.2 | PsTMT1 | 2 208 | 736 | 78.40 | 5.24 | 质膜 Plasma membrane | 1.80E-92 |
EVM0000294.1 | PsTMT2 | 2 226 | 742 | 79.99 | 5.09 | 质膜 Plasma membrane | 2.70E-96 |
EVM0025033.1 | PsTMT3 | 2 178 | 726 | 77.92 | 4.97 | 质膜 Plasma membrane | 4.80E-84 |
EVM0024253.1 | PsTMT4 | 2 217 | 739 | 79.07 | 4.56 | 质膜 Plasma membrane | 1.10E-90 |
EVM0028193.1 | PsSUC1 | 1 533 | 511 | 53.90 | 9.07 | 质膜 Plasma membrane | 3.10E-80 |
EVM0013799.1 | PsSUC2 | 1 827 | 609 | 65.44 | 7.03 | 质膜 Plasma membrane | 4.20E-94 |
EVM0001814.2 | PsSUC3 | 1 497 | 499 | 53.68 | 9.21 | 质膜 Plasma membrane | 5.10E-92 |
糖 Sugar | PsSUC1 | PsTMT1 | PsTMT4 | PsPMT2 | PsPMT4 | PsPMT6 | PsINT2 | PsINT4 |
---|---|---|---|---|---|---|---|---|
葡萄糖 Glucose | -0.93 | 0.74 | 0.66 | 0.11 | 0.51 | -0.95 | -0.87 | -0.87 |
果糖 Fructose | 0.61 | -0.21 | -0.23 | -0.01 | -0.31 | 0.65 | 0.24 | 0.52 |
蔗糖 Sucrose | 0.83 | -0.81 | -0.61 | -0.13 | -0.63 | 0.93 | 0.82 | 0.94 |
糖 Sugar | PsSFP4 | PsSFP5 | PsSTP1 | PsSTP11 | PsSTP12 | PsSTP16 | PsSTP17 | |
葡萄糖 Glucose | 0.81 | 0.80 | 0.18 | 0.84 | 0.52 | -0.31 | -0.81 | |
果糖 Fructose | -0.57 | -0.56 | 0.12 | -0.73 | -0.58 | 0.49 | 0.64 | |
蔗糖 Sucrose | -0.85 | -0.87 | -0.25 | -0.83 | -0.52 | 0.26 | 0.84 |
表3 ‘芙蓉李’果实不同发育时期糖含量与PsST家族基因表达量相关性
Table 3 Correlation evaluation between sugars in‘Furongli’fruit during development and PsSTs expression
糖 Sugar | PsSUC1 | PsTMT1 | PsTMT4 | PsPMT2 | PsPMT4 | PsPMT6 | PsINT2 | PsINT4 |
---|---|---|---|---|---|---|---|---|
葡萄糖 Glucose | -0.93 | 0.74 | 0.66 | 0.11 | 0.51 | -0.95 | -0.87 | -0.87 |
果糖 Fructose | 0.61 | -0.21 | -0.23 | -0.01 | -0.31 | 0.65 | 0.24 | 0.52 |
蔗糖 Sucrose | 0.83 | -0.81 | -0.61 | -0.13 | -0.63 | 0.93 | 0.82 | 0.94 |
糖 Sugar | PsSFP4 | PsSFP5 | PsSTP1 | PsSTP11 | PsSTP12 | PsSTP16 | PsSTP17 | |
葡萄糖 Glucose | 0.81 | 0.80 | 0.18 | 0.84 | 0.52 | -0.31 | -0.81 | |
果糖 Fructose | -0.57 | -0.56 | 0.12 | -0.73 | -0.58 | 0.49 | 0.64 | |
蔗糖 Sucrose | -0.85 | -0.87 | -0.25 | -0.83 | -0.52 | 0.26 | 0.84 |
品种 Cultivar | 葡萄糖 Glucose | 果糖 Fructose | 蔗糖 Sucrose | 总糖 Total soluble carbohydrates |
---|---|---|---|---|
芙蓉李 Furongli | 50.95 ± 3.07 a | 37.89 ± 3.68 a | 22.98 ± 3.95 ab | 111.82 ±10.7 a |
秋姬李 Qiujili | 48.08 ± 3.62 a | 38.34 ± 2.26 a | 15.11 ± 2.09 ab | 101.53 ±7.97 ab |
红李 Hongli | 39.65 ± 3.12 ab | 33.25 ± 3.84 ab | 4.02 ± 2.25 b | 76.92 ±9.21 b |
胭脂李 Yanzhili | 26.71 ± 2.62 b | 26.76 ± 3.20 ab | 25.92 ± 2.73 ab | 79.39 ±8.55 b |
华蜜大蜜李 Huami Damili | 25.57 ± 3.27 b | 25.73 ± 3.29 ab | 29.31 ± 4.17 a | 80.61 ±10.73 b |
白脆鸡麻李 Baicui Jimali | 23.31 ± 3.07 b | 16.83 ± 2.97 b | 22.99 ± 3.07 ab | 63.13 ±9.11 b |
红线李 Hongxianli | 20.90 ± 2.81 b | 24.79 ± 3.05 ab | 15.53 ± 1.15 ab | 61.22 ±7.01 b |
黑琥珀 Heihupo | 21.84 ± 2.15 b | 22.97 ± 3.15 b | 5.84 ± 0.51 b | 50.65 ±5.81 b |
表4 不同品种李果实可溶性糖含量分析
Table 4 Analysis of soluble carbohydrates contents in different plum varieties mg · g-1 FW
品种 Cultivar | 葡萄糖 Glucose | 果糖 Fructose | 蔗糖 Sucrose | 总糖 Total soluble carbohydrates |
---|---|---|---|---|
芙蓉李 Furongli | 50.95 ± 3.07 a | 37.89 ± 3.68 a | 22.98 ± 3.95 ab | 111.82 ±10.7 a |
秋姬李 Qiujili | 48.08 ± 3.62 a | 38.34 ± 2.26 a | 15.11 ± 2.09 ab | 101.53 ±7.97 ab |
红李 Hongli | 39.65 ± 3.12 ab | 33.25 ± 3.84 ab | 4.02 ± 2.25 b | 76.92 ±9.21 b |
胭脂李 Yanzhili | 26.71 ± 2.62 b | 26.76 ± 3.20 ab | 25.92 ± 2.73 ab | 79.39 ±8.55 b |
华蜜大蜜李 Huami Damili | 25.57 ± 3.27 b | 25.73 ± 3.29 ab | 29.31 ± 4.17 a | 80.61 ±10.73 b |
白脆鸡麻李 Baicui Jimali | 23.31 ± 3.07 b | 16.83 ± 2.97 b | 22.99 ± 3.07 ab | 63.13 ±9.11 b |
红线李 Hongxianli | 20.90 ± 2.81 b | 24.79 ± 3.05 ab | 15.53 ± 1.15 ab | 61.22 ±7.01 b |
黑琥珀 Heihupo | 21.84 ± 2.15 b | 22.97 ± 3.15 b | 5.84 ± 0.51 b | 50.65 ±5.81 b |
图6 荧光定量PCR分析4个糖转运蛋白基因在不同品种李果实中的表达量 FRL:芙蓉李;QJL:秋姬李;HL:红李;YZL:胭脂李;HMDML:华蜜大蜜李;BCJML:白脆鸡麻李;HXL:红线李;HHP:黑琥珀。
Fig. 6 Quantitative RT-PCR analysis of four sugar transporter genes in different plum cultivar FRL:Furongli;QJL:Qiujili;HL:Hongli;YZL:Yanzhili;HMDML:Huami Damili;BCJML:Baicui Jimali;HXL:Hongxianli;HHP:Heihupo.
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