兰科植物MYB转录因子研究进展

doi:10.16420/j.issn.0513-353x.2020-0843

摘要/Abstract

摘要：

介绍了MYB转录因子的结构及分类,并对其在兰科植物中类黄酮合成、花香调控、花器官形态建成和环境胁迫应答等方面的功能进行综述,以期为今后MYB分子机理研究和兰科植物新品种培育提供参考依据。

关键词: 兰科植物, MYB转录因子, 花青苷, 花器官形态, 非生物胁迫

Abstract:

This article systematically reviewed the structure and classification of MYB transcription factors,summarized their functions in flavonoid synthesis,floral aroma regulation,floral organ morphogenesis and environmental stress responses in orchids,as well as provided fundamental insights into future research of MYB molecular mechanism and the new varieties breeding of orchids.

Key words: Orchidaceae, MYB transcription factor, anthocyanin, floral organ morphology, abiotic stress

中图分类号:

S682.31

柯玉洁, 陈明堃, 马山虎, 欧悦, 王艺, 郑清冬, 刘仲健, 艾叶. 兰科植物MYB转录因子研究进展[J]. 园艺学报, 2021, 48(11): 2311-2320.

KE Yujie, CHEN Mingkun, MA Shanhu, OU Yue, WANG Yi, ZHENG Qingdong, LIU Zhongjian, AI Ye. Research Progress of MYB Transcription Factors in Orchidaceae[J]. Acta Horticulturae Sinica, 2021, 48(11): 2311-2320.

图/表 2

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功能 Function	来源植物 Original plant	基因 Gene	性状改变 Changed characteristic	参考文献 Reference
类黄酮调控 Flavonoid regulation	兰属品种 Cymbidium‘Mystique’	CyMYB1	激活CyDFR和CyANS的转录,促进花青苷积累 To activate CyDFR and CyANS transcription and promote anthocyanin accumulation	Nakatsuka et al.,2019
	小兰屿蝴蝶兰 Phalaenopsis equestris	PeMYB2, PeMYB11, PeMYB12	调控花萼和花瓣的色素着色 To regulate the pigmentation of sepals and petals	Hsu et al.,2015
	文心兰杂交种 Oncidium Gower Ramsey	OgMYB1	诱导唇瓣组织花青苷积累 To induce anthocyanin accumulation in lip tissue	Chiou & Yeh,2008
	石斛杂交种 Dendrobium hybrid	DhMYB2	导致白花瓣中花青苷的产生 To produce anthocyanin in white petals	Li et al.,2017
	西蕾丽蝴蝶兰 Phalaenopsis schilleriana	PsMYB	在西蕾丽蝴蝶兰紫色花中表达,在美丽蝴蝶兰白色花中不表达 MYB is expressed in purple flowers of Phalaenopsis schilleriana,but not in white flowers of P. amabilis	Ma et al.,2009
	卡特兰 Cattleya hybrid‘KOVA’	RcPCP1, RcPAP1, RcPAP2	过表达导致白色的花被分别变成淡黄色和紫红色 Overexpression of RcPCP1 or RcPAP1/2 result in white perianths that became slightly yellow or purple-red,respectively	Li et al.,2020
	蝴蝶兰‘大辣椒’ Phalaenopsis‘Big Chili’	MYBx1	抑制花青苷积累 To repress anthocyanin accumulation	Fu et al.,2019
	四川独蒜兰 Pleione limprichtii	PlMYB10	作为花青苷积累的抑制因子 To serve as a repressor of anthocyanin accumulation	Zhang et al.,2020
花香调控 Floral aroma regulation	兰属品种 Cymbidium‘Sael Bit’	CsMYB1	调节苯丙素或苯类的合成 To regulate the synthesis of phenylpropanoids or benzenes	Ramya et al.,2019
花香调控 Floral aroma regulation	蕙兰 Cymbidium faberi	CfMYB	激活CfAOC和CfJMT启动子表达来提高茉莉酸甲酯生物合成 To activate the expression of CfAOC and CfJMT promoters,and enhance methyl jasmonate biosynthesis	Xu et al.,2020
花器官形态调控 Floral organ morphology regulation	小兰屿蝴蝶兰 Phalaenopsis equestris	PeMYBs	能参与相应组织不同发育时期的调控 To participate in the regulation of growth process of corresponding tissues	王雪霁等,2018
	兰属品种 Cymbidium‘Sael Bit’	CsMYB1	MYB在花瓣与合蕊柱中表达量最高,在萼片与唇瓣中表达量最低 The highest expression in petals and column,but the lowest expression in sepals and lips	Ramya et al.,2019
	文心兰‘柠檬绿’ Oncidium hybridum	OnMYB106	在花苞里表达量最高 With the highest expression in flora buds	高玉莹,2018
	兰属品种 Cymbidium‘Mystique’	CyMYB1	不同发育时期花被片中表达量不同 The expression of MYB was different at growth process of tepals	Nakatsuka et al.,2019
	石斛杂交种 Dendrobium Bobby Messina × Dendrobium Chao Phraya	DhMYB1	参与石斛杂交种唇瓣表皮锥形细胞的发育 To participate in the growth of epidermal conical cells in the lip of Dendrobium orchid hybrid	Lau et al.,2015
环境胁迫应答 Environmental stress responses	铁皮石斛 Dendrobium officinale	DoMYB75	在拟南芥中的过表达会增加种子中水溶性多糖含量 Overexpression of MYB in Arabidopsis will increase water-soluble polysaccharide content in seeds	He et al.,2019
	石斛属杂交种 Dendrobium hybrid	DwMYB2	提高植物对铁缺失的敏感度 To enhance the sensitivity of plants to iron deficiency	Chen et al.,2006b
	小兰屿蝴蝶兰 Phalaenopsis equestris	PeMYB03, PeMYB53	可能与氧胁迫有关 Related to oxygen stress probably	王雪霁等,2018
	铁皮石斛 Dendrobium officinale	DoMYBR,DoMYB28, DoMYB29	分别在冷胁迫和盐胁迫时上调 To up-regulate under cold stress and salt stress respectively	He et al.,2019

功能 Function	来源植物 Original plant	基因 Gene	性状改变 Changed characteristic	参考文献 Reference
类黄酮调控 Flavonoid regulation	兰属品种 Cymbidium‘Mystique’	CyMYB1	激活CyDFR和CyANS的转录,促进花青苷积累 To activate CyDFR and CyANS transcription and promote anthocyanin accumulation	Nakatsuka et al.,2019
	小兰屿蝴蝶兰 Phalaenopsis equestris	PeMYB2, PeMYB11, PeMYB12	调控花萼和花瓣的色素着色 To regulate the pigmentation of sepals and petals	Hsu et al.,2015
	文心兰杂交种 Oncidium Gower Ramsey	OgMYB1	诱导唇瓣组织花青苷积累 To induce anthocyanin accumulation in lip tissue	Chiou & Yeh,2008
	石斛杂交种 Dendrobium hybrid	DhMYB2	导致白花瓣中花青苷的产生 To produce anthocyanin in white petals	Li et al.,2017
	西蕾丽蝴蝶兰 Phalaenopsis schilleriana	PsMYB	在西蕾丽蝴蝶兰紫色花中表达,在美丽蝴蝶兰白色花中不表达 MYB is expressed in purple flowers of Phalaenopsis schilleriana,but not in white flowers of P. amabilis	Ma et al.,2009
	卡特兰 Cattleya hybrid‘KOVA’	RcPCP1, RcPAP1, RcPAP2	过表达导致白色的花被分别变成淡黄色和紫红色 Overexpression of RcPCP1 or RcPAP1/2 result in white perianths that became slightly yellow or purple-red,respectively	Li et al.,2020
	蝴蝶兰‘大辣椒’ Phalaenopsis‘Big Chili’	MYBx1	抑制花青苷积累 To repress anthocyanin accumulation	Fu et al.,2019
	四川独蒜兰 Pleione limprichtii	PlMYB10	作为花青苷积累的抑制因子 To serve as a repressor of anthocyanin accumulation	Zhang et al.,2020
花香调控 Floral aroma regulation	兰属品种 Cymbidium‘Sael Bit’	CsMYB1	调节苯丙素或苯类的合成 To regulate the synthesis of phenylpropanoids or benzenes	Ramya et al.,2019
花香调控 Floral aroma regulation	蕙兰 Cymbidium faberi	CfMYB	激活CfAOC和CfJMT启动子表达来提高茉莉酸甲酯生物合成 To activate the expression of CfAOC and CfJMT promoters,and enhance methyl jasmonate biosynthesis	Xu et al.,2020
花器官形态调控 Floral organ morphology regulation	小兰屿蝴蝶兰 Phalaenopsis equestris	PeMYBs	能参与相应组织不同发育时期的调控 To participate in the regulation of growth process of corresponding tissues	王雪霁等,2018
	兰属品种 Cymbidium‘Sael Bit’	CsMYB1	MYB在花瓣与合蕊柱中表达量最高,在萼片与唇瓣中表达量最低 The highest expression in petals and column,but the lowest expression in sepals and lips	Ramya et al.,2019
	文心兰‘柠檬绿’ Oncidium hybridum	OnMYB106	在花苞里表达量最高 With the highest expression in flora buds	高玉莹,2018
	兰属品种 Cymbidium‘Mystique’	CyMYB1	不同发育时期花被片中表达量不同 The expression of MYB was different at growth process of tepals	Nakatsuka et al.,2019
	石斛杂交种 Dendrobium Bobby Messina × Dendrobium Chao Phraya	DhMYB1	参与石斛杂交种唇瓣表皮锥形细胞的发育 To participate in the growth of epidermal conical cells in the lip of Dendrobium orchid hybrid	Lau et al.,2015
环境胁迫应答 Environmental stress responses	铁皮石斛 Dendrobium officinale	DoMYB75	在拟南芥中的过表达会增加种子中水溶性多糖含量 Overexpression of MYB in Arabidopsis will increase water-soluble polysaccharide content in seeds	He et al.,2019
	石斛属杂交种 Dendrobium hybrid	DwMYB2	提高植物对铁缺失的敏感度 To enhance the sensitivity of plants to iron deficiency	Chen et al.,2006b
	小兰屿蝴蝶兰 Phalaenopsis equestris	PeMYB03, PeMYB53	可能与氧胁迫有关 Related to oxygen stress probably	王雪霁等,2018
	铁皮石斛 Dendrobium officinale	DoMYBR,DoMYB28, DoMYB29	分别在冷胁迫和盐胁迫时上调 To up-regulate under cold stress and salt stress respectively	He et al.,2019