‘白姜花’×‘金姜花’杂交F1代花色遗传分析及其相关SSR分子标记开发

doi:10.16420/j.issn.0513-353x.2021-0624

摘要/Abstract

摘要：

以‘白姜花’为母本,‘金姜花’为父本杂交获得的114株F₁杂交后代为材料,对唇瓣主色、唇瓣心部主色、花丝主色和花药主色等4个重要花色性状进行观测和分析,同时利用集团分离分析法开发花色相关分子标记。结果显示,114株F₁杂交后代花色分离广泛,唇瓣主色、唇瓣心部主色、花丝主色和花药主色的香农—维纳指数为0.57 ~ 1.58。唇瓣主色和唇瓣心部主色的L^*、a^*、b^*值呈连续分布。聚类分析可将114个F₁杂交后代分为橙色系和白色系两大类。BSA-SSR分析从191对引物中确定了HcgSSR676标记与花色性状紧密相关,该标记与白色系/橙色系,唇瓣主色黄度值（b^*₁）、唇瓣心部主色亮度值（L^*₂）、红度值（a^*₂）、黄度值（b^*₂）极显著相关,回归决定系数R²范围为7.92 ~ 23.19。

关键词: 姜花, 花色, 遗传分析, SSR, BSA

Abstract:

In this study,114 F₁ hybrids derived from the cross between Hedychium coronarium‘COR01’and H.‘Jin’were used as materials. Four important flower color-related traits including the dominant color of labellum,labellum heart,filament,and anther were observed and analysed. Meanwhile,bulked segregant analysis（BSA）was used to develop color-related molecular markers. The results showed that the flower color of 114 F₁ hybrids was widely separated,and the Shannon-Wiener index of the main color of labellum petal,labellum heart,filament,and anther ranged from 0.57 to 1.58. Colorimeter analysis indicated that the L^*,a^* and b^*values of the dominant color of labellum and labellum heart were continuously distributed. Cluster analysis revealed that 114 F₁ hybrids could be divided into white group and orange group. BSA-SSR analysis confirmed that the HcgSSR676 marker screened out from 191 primers pairs was closely related to flower color traits. The marker was significantly associated with the white/orange group,yellowness value of labellum dominant color（b^*₁）,brightness value（L^*₂）,redness value（a^*₂）,and yellowness value（b^*₂）of labellum heart dominant color,whose regression determination coefficient（R²）ranged from 7.92 to 23.19.

Key words: Hedychium, flower color, genetic analysis, SSR, BSA

中图分类号:

S682

周熠玮, 许国宇, 王琴, 严福龙, 玉云祎, 余让才, 范燕萍. ‘白姜花’×‘金姜花’杂交F₁代花色遗传分析及其相关SSR分子标记开发[J]. 园艺学报, 2021, 48(10): 1921-1933.

ZHOU Yiwei, XU Guoyu, WANG Qin, YAN Fulong, YU Yunyi, YU Rangcai, FAN Yanping. Genetic Analysis and Development of Associated SSR Markers of the Flower Color in F₁ Population of Hedychium coronarium‘COR01’× H. ‘Jin’[J]. Acta Horticulturae Sinica, 2021, 48(10): 1921-1933.

图/表 11

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引物编号 Primer number	SSR基序 SSR motif	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
HcgSSR119	（TTGCCA）₇	TTACTGCGACCACAGCAAAC	TTGATTTACTCGCTTCCGCT
HcgSSR649	（AAAATT）₆	GGATTGAAGTTAGAAGTTGATATTGGA	TGTGGTTTGAGATTAGGGGTG
HcgSSR676	（AT）₂₇	CTTGTTGATTCCACTTCCAAAA	ACCACCTCAGCAGGTACACA
HcgSSR926	（TA）₄₁	TCGCACATATAAGGGTGTGC	CCCAATCAATTTGAAAACCAA

引物编号 Primer number	SSR基序 SSR motif	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
HcgSSR119	（TTGCCA）₇	TTACTGCGACCACAGCAAAC	TTGATTTACTCGCTTCCGCT
HcgSSR649	（AAAATT）₆	GGATTGAAGTTAGAAGTTGATATTGGA	TGTGGTTTGAGATTAGGGGTG
HcgSSR676	（AT）₂₇	CTTGTTGATTCCACTTCCAAAA	ACCACCTCAGCAGGTACACA
HcgSSR926	（TA）₄₁	TCGCACATATAAGGGTGTGC	CCCAATCAATTTGAAAACCAA

观测部位 Observation position	姜花 Hedychium	比色卡 RHS	颜色 Color	数量（占比%） Count	H’ 香农—维纳指数 Shannon-Wiener index
唇瓣Labellum	♀	155D	白White
	♂	19C	橙黄Orange-yellow
	F₁	155B ~ NN155D	白White	95（83.33）	0.57
		158C ~ 158D	黄白Yellow-white	2（1.75）
		159A ~ 159D	橙白Orange-white	15（13.16）
		11D	黄Yellow	1（0.88）
		27A	橙Orange	1（0.88）
唇瓣心部	♀	2D	黄Yellow
Labellum heart	♂	28A	橙Orange
	F₁	145D ~ 150D	黄绿Yellow-green	42（39.47）	1.52
		1D ~ 13A	黄Yellow	14（12.28）
		15A ~ 23C	橙黄Orange-yellow	23（20.18）
		24A ~ 28B	橙Orange	20（17.54）
		30D ~ 35B	橙红Orange-red	15（13.16）
花丝Filament	♀	155B	白White
	♂	22B	橙黄Orange-yellow
	F₁	155A ~ NN155D	白White	27（23.68）	1.58
		4D ~ 14D	黄Yellow	14（12.28）
		14D ~ 23C	橙黄Orange-yellow	28（24.56）
		24A ~ 28D	橙Orange	21（18.42）
		30B ~ 35B	橙红Orange-red	24（21.05）
花药 Anther	♀	4D	白White
	♂	22B	橙黄Orange-yellow
	F₁	4D ~ 11D	黄Yellow	24（21.05）	1.34
		14D ~ 24C	橙黄Orange-yellow	35（30.70）
		24A ~ 28B	橙Orange	38（33.33）
		30A ~ 33D	橙红Orange-red	17（14.91）

观测部位 Observation position	姜花 Hedychium	比色卡 RHS	颜色 Color	数量（占比%） Count	H’ 香农—维纳指数 Shannon-Wiener index
唇瓣Labellum	♀	155D	白White
	♂	19C	橙黄Orange-yellow
	F₁	155B ~ NN155D	白White	95（83.33）	0.57
		158C ~ 158D	黄白Yellow-white	2（1.75）
		159A ~ 159D	橙白Orange-white	15（13.16）
		11D	黄Yellow	1（0.88）
		27A	橙Orange	1（0.88）
唇瓣心部	♀	2D	黄Yellow
Labellum heart	♂	28A	橙Orange
	F₁	145D ~ 150D	黄绿Yellow-green	42（39.47）	1.52
		1D ~ 13A	黄Yellow	14（12.28）
		15A ~ 23C	橙黄Orange-yellow	23（20.18）
		24A ~ 28B	橙Orange	20（17.54）
		30D ~ 35B	橙红Orange-red	15（13.16）
花丝Filament	♀	155B	白White
	♂	22B	橙黄Orange-yellow
	F₁	155A ~ NN155D	白White	27（23.68）	1.58
		4D ~ 14D	黄Yellow	14（12.28）
		14D ~ 23C	橙黄Orange-yellow	28（24.56）
		24A ~ 28D	橙Orange	21（18.42）
		30B ~ 35B	橙红Orange-red	24（21.05）
花药 Anther	♀	4D	白White
	♂	22B	橙黄Orange-yellow
	F₁	4D ~ 11D	黄Yellow	24（21.05）	1.34
		14D ~ 24C	橙黄Orange-yellow	35（30.70）
		24A ~ 28B	橙Orange	38（33.33）
		30A ~ 33D	橙红Orange-red	17（14.91）

观测部位 Observation site	指标 Colorimeter	白色系White group		橙色系Orange group		t统计值 t-statistic	P值 P-value
观测部位 Observation site	指标 Colorimeter	范围Range	均值Mean	范围Range	均值Mean	t统计值 t-statistic	P值 P-value
唇瓣Labellum	L^*₁	86.38 ~ 96.56	91.34	78.25 ~ 93.97	89.07	-4.54	1.42E-05^**
	a^*₁	-0.05 ~ 2.98	0.96	-0.50 ~ 12.30	1.70	2.11	0.037^*
	b^*₁	-2.88 ~ 9.99	1.44	-0.58 ~ 31.31	11.67	9.66	1.97E-16^**
唇瓣心部 Labellum heart	L^*₂	82.09 ~ 90.49	86.75	55.16 ~ 83.71	73.11	-14.10	1.43E-26^**
	a^*₂	-2.03 ~ 2.97	-0.22	0.79 ~ 47.55	16.47	10.72	7.11E-19^**
	b^*₂	2.96 ~ 22.79	10.85	19.56 ~ 62.43	41.60	20.05	7.48E-39^**

‘白姜花’×‘金姜花’杂交F₁代花色遗传分析及其相关SSR分子标记开发

Genetic Analysis and Development of Associated SSR Markers of the Flower Color in F₁ Population of Hedychium coronarium‘COR01’× H. ‘Jin’

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性状Trait		相关系数Correlation coefficient	R²
白色系/橙色系 White group/orange group		-0.47^**	21.56
唇瓣Labellum	L^*₁	0.22^*	3.81
	a^*₁	-0.11	3.62
	b^*₁	-0.33^**	10.09
唇瓣心部 Labellum heart	L^*₂	0.34^**	10.69
	a^*₂	-0.30^**	7.92
	b^*₂	-0.49^**	23.19

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