园艺学报 ›› 2021, Vol. 48 ›› Issue (8): 1457-1469.doi: 10.16420/j.issn.0513-353x.2020-0538
收稿日期:
2021-01-17
修回日期:
2021-04-06
出版日期:
2021-08-25
发布日期:
2021-09-06
通讯作者:
石胜友
E-mail:ssy7299@163.com
基金资助:
YANG Weihai1, ZENG Lizhen1, XIAO Qiusheng1, SHI Shengyou2,*()
Received:
2021-01-17
Revised:
2021-04-06
Online:
2021-08-25
Published:
2021-09-06
Contact:
SHI Shengyou
E-mail:ssy7299@163.com
摘要:
为探究“饥饿胁迫”诱导龙眼果实脱落的调控机制,以‘储良’龙眼为试材,在果实发育早期对结果母枝进行环剥去叶处理,模拟中断果穗碳素供应所引发的饥饿胁迫,测定处理后0、1、3、5、6 d果皮及果柄离区的糖与脱落酸(ABA)含量及相关基因的表达水平。结果表明:果实在处理后5 ~ 6 d发生大量脱落;果皮中可溶性总糖及各组分(葡萄糖、果糖、蔗糖)含量显著下降,以3 d内的降幅较大,且蔗糖与葡萄糖的降幅显著大于果糖;离区的可溶性总糖及蔗糖含量也发生类似的变化。处理后ABA含量在果皮中的变化不明显,但在离区中显著增加。饥饿胁迫总体上减弱了果皮与离区中糖与ABA相关基因的表达,在果实脱落早期有关蔗糖代谢基因(NI、SS1、SPS1)、糖信号基因(HXK2、TPS1、SnRK2)及ABA代谢基因(AAO3、NCED、BG)的表达均明显下降,但在大量落果时期NI、SS1、HXK2、SnRK2、AAO3、NCED等基因的表达一度增强,使得蔗糖急剧减少,导致糖信号的产生和ABA的积累。以上结果说明,糖与ABA之间的信息交流可能与碳水化合物饥饿胁迫诱导的龙眼果实脱落有关。
中图分类号:
杨为海, 曾利珍, 肖秋生, 石胜友. 饥饿胁迫下龙眼落果与果皮和离区糖、ABA及相关基因表达的变化[J]. 园艺学报, 2021, 48(8): 1457-1469.
YANG Weihai, ZENG Lizhen, XIAO Qiusheng, SHI Shengyou. Changes of Fruit Abscission and Carbohydrate,ABA and Related Genes Expression in the Pericarp and Fruit Abscission Zone of Longan Under Starvation Stress[J]. Acta Horticulturae Sinica, 2021, 48(8): 1457-1469.
基因Gene | 正向引物(5′-3′)Forward primer sequence | 反向引物(5′-3′)Reverse primer sequence | |
---|---|---|---|
Actin | TGCTATCCTTCGGTTGGACC | CGGACGATTTCCCGTTCAG | |
NI | GATTTTGAGGTTATGTCTCGCTG | GATTGTTCAGTGCTCGGATAAGA | |
SS1 | AATTCCTTAATCGCCACCTCT | AGCATCATATTCTTGCCCTTG | |
SPS1 | ATGGTGACAAAAATGAAGGTGG | TGCCTTTTGTGCTCCTCCA | |
HXK2 | AGTGCTTTTGAAGATGGCTGAA | ATCCAACGGCTTGAAGACCT | |
TPS1 | GGTCTTTGTGGGAGGCGTAT | CGGACTGTGAAGAAAGAACCC | |
SnRK2 | GGCCAAAAGATTGATGAACATG | TTGCCTCGTCCTCGCTAAA | |
BG | AAAGGAACCCCAATGAGACC | ACCGTCATAGGTGAAAATCCC | |
AAO3 | GACTGGGGTGCTTTACACTTTG | CGACTTCCATAGATAGCCTAGACG | |
NCED | ACTTGTGGAATGCTTGGGAG | CCTGATTTCGGACAAAACACTC |
表1 实时荧光定量PCR分析的引物序列
Table 1 The primer sequences used for qRT-PCR analysis
基因Gene | 正向引物(5′-3′)Forward primer sequence | 反向引物(5′-3′)Reverse primer sequence | |
---|---|---|---|
Actin | TGCTATCCTTCGGTTGGACC | CGGACGATTTCCCGTTCAG | |
NI | GATTTTGAGGTTATGTCTCGCTG | GATTGTTCAGTGCTCGGATAAGA | |
SS1 | AATTCCTTAATCGCCACCTCT | AGCATCATATTCTTGCCCTTG | |
SPS1 | ATGGTGACAAAAATGAAGGTGG | TGCCTTTTGTGCTCCTCCA | |
HXK2 | AGTGCTTTTGAAGATGGCTGAA | ATCCAACGGCTTGAAGACCT | |
TPS1 | GGTCTTTGTGGGAGGCGTAT | CGGACTGTGAAGAAAGAACCC | |
SnRK2 | GGCCAAAAGATTGATGAACATG | TTGCCTCGTCCTCGCTAAA | |
BG | AAAGGAACCCCAATGAGACC | ACCGTCATAGGTGAAAATCCC | |
AAO3 | GACTGGGGTGCTTTACACTTTG | CGACTTCCATAGATAGCCTAGACG | |
NCED | ACTTGTGGAATGCTTGGGAG | CCTGATTTCGGACAAAACACTC |
图1 环剥去叶处理对龙眼果实脱落的影响 * 表示处理与对照相比差异显著(α = 0.05)。下同。
Fig. 1 Effect of girdling and defoliation treatment on fruitlet abscission of longan * indicated significant difference compared with the control(α = 0.05). The same below.
图2 环剥去叶处理后龙眼果皮中可溶性总糖与各组分含量的变化
Fig. 2 Changes in the contents of total soluble sugar and its composition in the longan pericarp after girdling and defoliation treatment
图3 环剥去叶处理后龙眼果柄离区中可溶性总糖与各组分含量的变化
Fig. 3 Changes in the contents of total soluble sugar and its composition in the pedicel abscission zone of longan after girdling and defoliation treatment
图4 环剥去叶处理后龙眼果皮(A)及果柄离区(B)中ABA含量的变化
Fig. 4 Changes of ABA content in the pericarp(A)and pedicel abscission zone(B)of longan after girdling and defoliation treatment
图5 环剥去叶处理后龙眼果皮中糖代谢与信号转导以及ABA代谢相关基因的相对表达量变化
Fig. 5 Changes in the expression levels of genes involved in sugar metabolism and signaling and ABA metabolism in the pericarp of longan after girdling and defoliation treatment
图6 环剥去叶处理后龙眼果实离区中糖代谢与信号转导以及ABA代谢相关基因的相对表达量变化
Fig. 6 Changes in the expression levels of genes involved in sugar metabolism and signaling and ABA metabolism in the fruit abscission zone of longan after girdling and defoliation treatment
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