番茄节间长度基因IL10和IL11的鉴定和功能验证

doi:10.16420/j.issn.0513-353x.2021-0206

摘要/Abstract

摘要：

通过对266份番茄核心种质资源第3穗果实处节间长度进行测定,进行全基因组关联分析,共鉴定7个与节间长度相关的QTL位点。对10和11号染色体中的显著性SNP进行候选基因分析,进一步挖掘控制番茄节间长度的主效基因IL10（Forkhead-associated domain protein）和IL11（Auxin-regulated protein）。以‘Ailsa Craig’番茄为背景材料,通过农杆菌介导的方法进行遗传转化,获得了11株IL10基因沉默、10株IL11基因沉默的株系,其节间长度比野生型短。综上所述,IL10和IL11为控制番茄节间长度的关键基因,对番茄节间长度起正向调控作用。

关键词: 番茄, 茎秆, 节间长度, 全基因组关联分析, 候选基因

Abstract:

We measured and analyzed internode length at the third panicle of 266 tomato accessions. Seven QTL related to tomato internode length were identified by genome-wide association study. Candidate genes were analyzed based on the significant SNPs in chromosomes 10 and 11. The IL10（Forkhead- associated domain protein）and IL11（Auxin-regulated protein）related to the internode length were further excavated. Tomato‘Ailsa Craig’was used as the background material. 11 IL10-RNAi lines and 10 IL11-RNAi lines were obtained by Agrobacterium-mediated transformation. The internode length of IL10- and IL11-silencing transgenic lines was significantly shorter than that of the wild type. To sum up,IL10 and IL11 are the key genes that positively regulates tomato internode length.

Key words: tomato, stem, internode length, genome-wide association study, candidate gene

中图分类号:

S641.2

刘根忠, 石春美, 于会洋, 王莹, 陈卫芳, 尚乐乐, 张余洋, 叶志彪. 番茄节间长度基因IL10和IL11的鉴定和功能验证[J]. 园艺学报, 2021, 48(7): 1340-1348.

LIU Genzhong, SHI Chunmei, YU Huiyang, WANG Ying, CHEN Weifang, SHANG Lele, ZHANG Yuyang, YE Zhibiao. Identification and Functional Verification of Tomato Stem Internode Length Genes IL10 and IL11[J]. Acta Horticulturae Sinica, 2021, 48(7): 1340-1348.

图/表 8

参考文献 29

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基因 Gene	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
IL11 for RNAi	GGGGACAAGTTTGTACAAAAAAGCAGGCTAAATTGGAAATAAGGGAGAAGGAGAT	GGGGACCACTTTGTACAAGAAAGCTGGGTGGCTAGTGCTTCCACCCCAAC
IL10 for RNAi	GGGGACAAGTTTGTACAAAAAAGCAGGCTGAGATATTGAAGTAGAACTTGTCACCTTTT	GGGGACCACTTTGTACAAGAAAGCTGGGTAGGACTTCACTAAGATTCTAAGCACTCTCT
IL11 for qRT-PCR	ATGGCGACATTTGTTAGAGCAAA	GTTCCGATTTGAGCTTATGATTTTG
IL10 for qRT-PCR	GAACAACAGGAGAATATCACAGCC	GGATACTGGAGATATGATTGCTTGA

基因 Gene	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
IL11 for RNAi	GGGGACAAGTTTGTACAAAAAAGCAGGCTAAATTGGAAATAAGGGAGAAGGAGAT	GGGGACCACTTTGTACAAGAAAGCTGGGTGGCTAGTGCTTCCACCCCAAC
IL10 for RNAi	GGGGACAAGTTTGTACAAAAAAGCAGGCTGAGATATTGAAGTAGAACTTGTCACCTTTT	GGGGACCACTTTGTACAAGAAAGCTGGGTAGGACTTCACTAAGATTCTAAGCACTCTCT
IL11 for qRT-PCR	ATGGCGACATTTGTTAGAGCAAA	GTTCCGATTTGAGCTTATGATTTTG
IL10 for qRT-PCR	GAACAACAGGAGAATATCACAGCC	GGATACTGGAGATATGATTGCTTGA