樱桃种质SCoT分子标记与叶片表型性状关联分析

doi:10.16420/j.issn.0513-353x.2020-0262

摘要/Abstract

摘要：

利用本研究中建立的樱桃SCoT标记分析体系,进行适宜引物筛选,进而对12个樱桃品种进行SCoT分子标记、叶片表型性状遗传多样性及两者关联分析。结果显示：从102条SCoT标记引物中筛选出46条适宜樱桃遗传分析的标记引物,筛选率达45.1%;参试的18条SCoT引物共获得等位位点数77个,其中多态性位点65个,多态性比率84.4%;12个樱桃品种的表型叶片性状变异系数为11.49% ~ 36.93%,叶片性状与SCoT标记聚类分析结果基本一致;Pearson’s 相关分析表明,共有54个标记位点与12个性状呈显著或极显著相关;多元线性逐步回归分析获得了8个与叶片性状极显著相关的特异标记位点,解释了叶片性状47.8% ~ 100.0%的变化;对8个关联标记进行序列比对分析,有5个标记能在樱桃基因组中找到同源序列,相似性均达75%以上,并能明确其染色体位置,其中4个标记能够在NCBI中比对到具有功能注释的同源序列,另3个标记未比对到同源序列。

关键词: 樱桃, SCoT标记, 叶片性状, 关联分析

Abstract:

Based on the established SCoT analysis system,the suitable primers were screened,and then SCoT molecular markers,genetic diversity of leaf traits and their correlation were analyzed in 12 cherry varieties. The results showed that 46 marker primers being suitable for cherry genetic analysis were selected from 102 SCoT marker primers,with a screening rate of 45.1%. A total of 77 allele points were obtained from 18 SCoT primers,including 65 polymorphic loci,with a polymorphism rate of 84.4%. The variation coefficient of leaf traits of 12 varieties was 11.49%-36.93%,which was basically consistent with the results of SCoT marker clustering analysis. Pearson’s correlation analysis showed that a total of 54 marker sites were significantly or extremely significantly correlated with 12 leaf traits. Through the multiple linear stepwise regression analysis,eight specific marker sites related to leaf traits were obtained, which explained the change of 47.8%-100.0% of leaf traits. Sequence analysis of eight association marker sites showed that five markers could find homologous sequences in cherry genome;the similarity was more than 75%;and their chromosome positions could be identified. Among them,four markers could find homologous sequences with functional annotation in NCBI,and three markers could not find homologous sequences.

Key words: cherry, SCoT markers, leaf traits, association analysis

中图分类号:

S662.5

彭芳芳, 龙治坚, 魏召新, 李勋兰, 罗友进, 韩国辉. 樱桃种质SCoT分子标记与叶片表型性状关联分析[J]. 园艺学报, 2021, 48(2): 325-335.

PENG Fangfang, LONG Zhijian, WEI Zhaoxin, LI Xunlan, LUO Youjin, HAN Guohui. Association Analysis of SCoT Markers and Leaf Phenotypic Traits in Cherry Germplasm[J]. Acta Horticulturae Sinica, 2021, 48(2): 325-335.

图/表 10

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所属种 Genus	品种 Cultivar	来源 Source
欧洲甜樱桃 Prunus avium L.	雷尼 Rainier	宾库 × 先锋 Bing × Van
	艳阳 Sunburst	先锋 × 斯特拉 Van × Stella
	拉宾斯 Lapins	先锋 × 斯特拉 Van × Stella
	早大果 Крупноплодная	乌克兰 Ukraine
	勃兰特 Burlat	法国 France
	龙冠 Longguan	那翁 × 大紫 Royal Ann（Napoleon）× Black Tartarian
	丽特 Lite	美国 USA
	红灯 Hongdeng	那翁 × 黄玉 Royal Ann（Napoleon）× Huangyu
	秦樱1号 Qinying 1	勃兰特自然突变种 Burlat's natural mutant
中国甜樱桃 P. pscudocerasus Lindl.	玛瑙红 Manaohong	中华酸樱桃实生变异 Variety of Chinese acid cherry
	红宝石 Hongbaoshi	重庆 Chongqing
	乌皮樱桃 WupiYingtao	重庆 Chongqing

所属种 Genus	品种 Cultivar	来源 Source
欧洲甜樱桃 Prunus avium L.	雷尼 Rainier	宾库 × 先锋 Bing × Van
	艳阳 Sunburst	先锋 × 斯特拉 Van × Stella
	拉宾斯 Lapins	先锋 × 斯特拉 Van × Stella
	早大果 Крупноплодная	乌克兰 Ukraine
	勃兰特 Burlat	法国 France
	龙冠 Longguan	那翁 × 大紫 Royal Ann（Napoleon）× Black Tartarian
	丽特 Lite	美国 USA
	红灯 Hongdeng	那翁 × 黄玉 Royal Ann（Napoleon）× Huangyu
	秦樱1号 Qinying 1	勃兰特自然突变种 Burlat's natural mutant
中国甜樱桃 P. pscudocerasus Lindl.	玛瑙红 Manaohong	中华酸樱桃实生变异 Variety of Chinese acid cherry
	红宝石 Hongbaoshi	重庆 Chongqing
	乌皮樱桃 WupiYingtao	重庆 Chongqing

引物名称 Primer name	引物序列（5′-3′） Primer sequence	引物名称 Primer name	引物序列（5′-3′） Primer sequence
SCoT02	CAACAATGGCTACCACCC	SCoT52	CAACATGGCTACCACTCG
SCoT03	CAACAATGGCTACCACCG	SCoT53	CAACATGGCTACCACTGC
SCoT04	CAACAATGGCTACCACCT	SCoT54	CAACATGGCTACCACTGA
SCoT05	CAACAATGGCTACCACGA	SCoT55	CAACATGGCTACCACAGC
SCoT14	ACGACATGGCGACCACGC	SCoT56	CCATGGCTACCACTGGCA
SCoT16	ACCATGGCTACCACCGAC	SCoT61	ACAATGGCTACCACCGCG
SCoT17	ACCATGGCTACCACCGAG	SCoT62	ACAATGGCTACCACTCAG
SCoT19	ACCATGGCTACCACCGGC	SCoT65	ACAATGGCTACCACTGGC
SCoT21	ACGACATGGCGACCCACA	SCoT66	CAATGGCTACCACTACAG
SCoT25	ACCATGGCTACCACCGGG	SCoT68	CAATGGCTACCACTAGCG
SCoT26	ACCATGGCTACCACCGTC	SCoT72	CAATGGCTACCACTAACG
SCoT31	CCATGGCTACCACCGCCT	SCoT75	CAATGGCTACCACTGACA
SCoT34	ACCATGGCTACCACCGCA	SCoT76	CAATGGCTACCATTAGCG
SCoT35	CATGGCTACCACCGGCCC	SCoT81	ACAATGGCTACCACTGCC
SCoT36	GCAACAATGGCTACCACC	SCoT83	ACAATGGCTACCACTGCG
SCoT41	ACCATGGCTACCACCGCA	SCoT86	ACAATGGCTACCACTGCA
SCoT42	ACCATGGCTACCACCAGC	SCoT88	ACAATGGCTACCACCAGC
SCoT46	ACCATGGCTACCACGGTC	SCoT89	ACAATGGCTACCACTACC
SCoT47	ACCATGGCTACCACGGCA	SCoT92	ACAATGGCTACCACTAGG
SCoT48	ACCATGGCTACCACGGAC	SCoT94	ACAATGGCTACCACCACA
SCoT49	ACCATGGCTACCAGCGAG	SCoT96	ACCATGGCTACCACGGAG
SCoT50	ACCATGGCTACCAGCGCA	SCoT97	ACCATGGCTACCACGGGC
SCoT51	CAACATGGCTACCACCAC	SCoT101	ACCATGGCTACCAGCGGC

引物名称 Primer name	引物序列（5′-3′） Primer sequence	引物名称 Primer name	引物序列（5′-3′） Primer sequence
SCoT02	CAACAATGGCTACCACCC	SCoT52	CAACATGGCTACCACTCG
SCoT03	CAACAATGGCTACCACCG	SCoT53	CAACATGGCTACCACTGC
SCoT04	CAACAATGGCTACCACCT	SCoT54	CAACATGGCTACCACTGA
SCoT05	CAACAATGGCTACCACGA	SCoT55	CAACATGGCTACCACAGC
SCoT14	ACGACATGGCGACCACGC	SCoT56	CCATGGCTACCACTGGCA
SCoT16	ACCATGGCTACCACCGAC	SCoT61	ACAATGGCTACCACCGCG
SCoT17	ACCATGGCTACCACCGAG	SCoT62	ACAATGGCTACCACTCAG
SCoT19	ACCATGGCTACCACCGGC	SCoT65	ACAATGGCTACCACTGGC
SCoT21	ACGACATGGCGACCCACA	SCoT66	CAATGGCTACCACTACAG
SCoT25	ACCATGGCTACCACCGGG	SCoT68	CAATGGCTACCACTAGCG
SCoT26	ACCATGGCTACCACCGTC	SCoT72	CAATGGCTACCACTAACG
SCoT31	CCATGGCTACCACCGCCT	SCoT75	CAATGGCTACCACTGACA
SCoT34	ACCATGGCTACCACCGCA	SCoT76	CAATGGCTACCATTAGCG
SCoT35	CATGGCTACCACCGGCCC	SCoT81	ACAATGGCTACCACTGCC
SCoT36	GCAACAATGGCTACCACC	SCoT83	ACAATGGCTACCACTGCG
SCoT41	ACCATGGCTACCACCGCA	SCoT86	ACAATGGCTACCACTGCA
SCoT42	ACCATGGCTACCACCAGC	SCoT88	ACAATGGCTACCACCAGC
SCoT46	ACCATGGCTACCACGGTC	SCoT89	ACAATGGCTACCACTACC
SCoT47	ACCATGGCTACCACGGCA	SCoT92	ACAATGGCTACCACTAGG
SCoT48	ACCATGGCTACCACGGAC	SCoT94	ACAATGGCTACCACCACA
SCoT49	ACCATGGCTACCAGCGAG	SCoT96	ACCATGGCTACCACGGAG
SCoT50	ACCATGGCTACCAGCGCA	SCoT97	ACCATGGCTACCACGGGC
SCoT51	CAACATGGCTACCACCAC	SCoT101	ACCATGGCTACCAGCGGC

引物名称 Primer name	等位位点数 Number of alleles	多态性位点数 Number of polymorphic alleles	多态性比率/% Ratio of olymorphic alleles	引物名称 Primer name	等位位点数 Number of alleles	多态性位点数 Number of polymorphic alleles	多态性比率/% Ratio of polymorphic alleles
SCoT2	3	2	66.7	SCoT31	3	2	66.7
SCoT3	4	4	100	SCoT35	4	2	50.0.
SCoT4	4	4	100	SCoT36	4	3	75
SCoT5	4	4	100	SCoT42	5	3	60
SCoT16	4	2	50	SCoT47	4	4	100
SCoT17	9	9	100	SCoT49	6	6	100
SCoT19	2	1	50	SCoT50	8	7	87.5
SCoT21	2	2	100	SCoT54	4	4	100
SCoT25	4	3	75	总计Total	77	65	84.4
SCoT26	3	3	100