园艺学报 ›› 2021, Vol. 48 ›› Issue (1): 96-106.doi: 10.16420/j.issn.0513-353x.2020-0212
付瀚森1,2, 张亚雯3, 赵阳阳1, 罗婷婷1, 邓慧杰1, 孟晨伟1, 王彩云1,2,*()
收稿日期:
2020-09-16
修回日期:
2020-12-03
出版日期:
2021-01-25
发布日期:
2021-01-29
通讯作者:
王彩云
E-mail:248361509@qq.com
基金资助:
FU Hansen1,2, ZHANG Yawen3, ZHAO Yangyang1, LUO Tingting1, DENG Huijie1, MENG Chenwei1, WANG Caiyun1,2,*()
Received:
2020-09-16
Revised:
2020-12-03
Online:
2021-01-25
Published:
2021-01-29
Contact:
WANG Caiyun
E-mail:248361509@qq.com
摘要:
菊花绿色品种相对稀少,育种难度较大,遗传机制报道较少。以舌状花绿色、平瓣的栽培菊(Chrysanthemum × morifolium)‘绿叮当’为母本,与舌状花白色、平瓣的野生种毛华菊(C. vestitum)进行杂交,将其秋季开花的453株后代作为遗传群体,选取舌状花的色相角h值和色相a*值、舌状花瓣数和花序直径这4个性状进行杂种优势分析和主基因 + 多基因混合遗传分析。结果表明:(1)这些性状呈连续变化,有极显著的中亲杂种优势,舌状花的色相角和花序直径有极显著的超亲优势;(2)色相角h值、色相a*值和舌状花瓣数均由两对加性—显性—上位性主基因控制,其主基因遗传率分别为38.74%、90.34%和76.20%;花序直径由一对加性—显性主基因控制,其主基因遗传率为41.20%。此外,杂交后代舌状花大多为平瓣,与双亲一致,少量个体出现管瓣与匙瓣。舌状花的色相角h值、舌状花瓣数及花序直径都分别与舌状花色相a*值显著相关。由此可见,在这个绿色菊花品种与野生近缘种杂交的拟测交F2代群体中,舌状花的色相a*值主基因遗传率高,并与其他花部性状显著相关。
中图分类号:
付瀚森, 张亚雯, 赵阳阳, 罗婷婷, 邓慧杰, 孟晨伟, 王彩云. 菊花‘绿叮当’与毛华菊杂交后代花部性状杂种优势与混合遗传分析[J]. 园艺学报, 2021, 48(1): 96-106.
FU Hansen, ZHANG Yawen, ZHAO Yangyang, LUO Tingting, DENG Huijie, MENG Chenwei, WANG Caiyun. Heterosis and Mixed Inheritance Analysis of Flower Head Traits in the Progeny of the Cross Between Chrysanthemum × morifolium ‘Lüdingdang’and Chrysanthemum vestitum[J]. Acta Horticulturae Sinica, 2021, 48(1): 96-106.
图1 杂交亲本(A)和杂交后代群体(B ~ E)花部性状表型 A:亲本菊花‘绿叮当’(♀)和毛华菊(♂);B:匙瓣和管瓣;C:舌状花瓣数的变化(花型);D:绿色程度的变化;E:花序直径的变化。
Fig. 1 Flower head traits of parents(A)and the progeny population(B-E)of Chrysanthemum × morifolium‘Lüdingdang’and C. vestitum A:C. × morifolium‘Lüdingdang’(♀)and C. vestitum(♂);B:Spoon type and tube type florets;C:Different numbers of florets(flower types);D:Different green levels;E:Different flower head diameter.
父母本 Parents | 舌状花 Ray floret | 花序直径/cm Flower head diameter | |||
---|---|---|---|---|---|
a | h/° | 花瓣数 Numbers of ray florets | 瓣型 Type of the ray florets | ||
菊花‘绿叮当’(♀)C. × morifolium‘Lüdingdang’ C. × morifolium‘Lüdingdang’ | -10.71 | 99.51 | 232.33 | 平瓣 Flat type | 4.00 |
毛华菊(♂)C. vestitum | -0.77 | 100.22 | 17.00 | 平瓣 Flat type | 3.47 |
表1 杂交双亲花部性状统计
Table 1 Flower head traits of the parents
父母本 Parents | 舌状花 Ray floret | 花序直径/cm Flower head diameter | |||
---|---|---|---|---|---|
a | h/° | 花瓣数 Numbers of ray florets | 瓣型 Type of the ray florets | ||
菊花‘绿叮当’(♀)C. × morifolium‘Lüdingdang’ C. × morifolium‘Lüdingdang’ | -10.71 | 99.51 | 232.33 | 平瓣 Flat type | 4.00 |
毛华菊(♂)C. vestitum | -0.77 | 100.22 | 17.00 | 平瓣 Flat type | 3.47 |
性状 Trait | 最大值 Max | 最小值 Min | 极值 Range | 平均值 Mean | 标准差 SD | 变异系数/% CV | 偏度 Skewness | 峰度 Kurtosis |
---|---|---|---|---|---|---|---|---|
h/° | 116.19 | 76.83 | 39.36 | 106.19 | 3.94 | 3.71 | -2.38 | 12.41 |
a* | 1.62 | -11.16 | 12.78 | -4.46 | 2.55 | -57.23 | 0.61 | 0.84 |
舌状花瓣数 Number of ray florets | 109.33 | 18.00 | 91.33 | 40.65 | 17.61 | 43.33 | 1.33 | 1.86 |
花序直径/cm Flower head diameter | 7.47 | 2.93 | 4.54 | 4.52 | 0.70 | 15.48 | 0.56 | 0.52 |
表2 菊花‘绿叮当’与毛华菊杂交后代群体花部性状特征值
Table 2 Flower head traits values in the progeny of Chrysanthemum × morifolium‘Lüdingdang’and C. vestitum
性状 Trait | 最大值 Max | 最小值 Min | 极值 Range | 平均值 Mean | 标准差 SD | 变异系数/% CV | 偏度 Skewness | 峰度 Kurtosis |
---|---|---|---|---|---|---|---|---|
h/° | 116.19 | 76.83 | 39.36 | 106.19 | 3.94 | 3.71 | -2.38 | 12.41 |
a* | 1.62 | -11.16 | 12.78 | -4.46 | 2.55 | -57.23 | 0.61 | 0.84 |
舌状花瓣数 Number of ray florets | 109.33 | 18.00 | 91.33 | 40.65 | 17.61 | 43.33 | 1.33 | 1.86 |
花序直径/cm Flower head diameter | 7.47 | 2.93 | 4.54 | 4.52 | 0.70 | 15.48 | 0.56 | 0.52 |
图2 菊花‘绿叮当’与毛华菊杂交后代群体各花部性状的频次及分布 实线为理论分布,虚线为成分分布。
Fig. 2 Frequency distributions for flower head traits in the progeny of Chrysanthemu × morifolium‘Lüdingdang’and C. vestitum Solid lines showed the theoretical distributions,and dotted lines showed the component distributions.
性状 Trait | ♀ 菊花‘绿叮当’ C. × morifolium ‘Lüdingdang’ | ♂ 毛华菊 C. vestitum | 中亲值 MPV | 杂交后代 The progeny population | ||||
---|---|---|---|---|---|---|---|---|
群体平均 数Fm | 中亲优势 Hm | 中亲优势率/% RHm | 超亲优势 H | 超亲优势率/% RH | ||||
h /° | 99.51 | 100.22 | 99.87 | 106.19 | 6.32** | 6.33 | 5.97** | 5.96 |
a* | -10.71 | -0.77 | -5.74 | -4.46 | 1.28** | -22.32 | — | — |
舌状花瓣数 Number of ray florets | 232.33 | 17.00 | 124.67 | 40.65 | -84.02** | -67.39 | — | — |
花序直径/cm Flower head diameter | 4.00 | 3.47 | 3.74 | 4.52 | 0.78** | 20.81 | 0.52** | 14.94 |
表3 菊花‘绿叮当’与毛华菊杂交后代群体花部性状的杂种优势表现
Table 3 Heterosis of flower head traits in the progeny of Chrysanthemu × morifolium‘Lüdingdang’and C. vestitum
性状 Trait | ♀ 菊花‘绿叮当’ C. × morifolium ‘Lüdingdang’ | ♂ 毛华菊 C. vestitum | 中亲值 MPV | 杂交后代 The progeny population | ||||
---|---|---|---|---|---|---|---|---|
群体平均 数Fm | 中亲优势 Hm | 中亲优势率/% RHm | 超亲优势 H | 超亲优势率/% RH | ||||
h /° | 99.51 | 100.22 | 99.87 | 106.19 | 6.32** | 6.33 | 5.97** | 5.96 |
a* | -10.71 | -0.77 | -5.74 | -4.46 | 1.28** | -22.32 | — | — |
舌状花瓣数 Number of ray florets | 232.33 | 17.00 | 124.67 | 40.65 | -84.02** | -67.39 | — | — |
花序直径/cm Flower head diameter | 4.00 | 3.47 | 3.74 | 4.52 | 0.78** | 20.81 | 0.52** | 14.94 |
性状 Trait | h | a* | 舌状花瓣数 Number of ray florets | 花序直径 Flower head diameter |
---|---|---|---|---|
h | 1 | — | — | — |
a* | 0.266** | 1 | — | — |
舌状花瓣数 Number of ray florets | -0.048 | -0.103* | 1 | — |
花序直径 Flower head diameter | 0.133** | 0.483** | -0.059 | 1 |
表4 菊花‘绿叮当’与毛华菊杂交后代4个花部性状的Pearson相关系数
Table 4 Pearson’s correlation coefficients among the four traits in the progeny of Chrysanthemu × morifolium‘Lüdingdang’and C. vestitum
性状 Trait | h | a* | 舌状花瓣数 Number of ray florets | 花序直径 Flower head diameter |
---|---|---|---|---|
h | 1 | — | — | — |
a* | 0.266** | 1 | — | — |
舌状花瓣数 Number of ray florets | -0.048 | -0.103* | 1 | — |
花序直径 Flower head diameter | 0.133** | 0.483** | -0.059 | 1 |
模型编码 Model code | h | a* | 舌状花瓣数 Number of ray florets | 花序直径/cm Flower head diameter |
---|---|---|---|---|
0MG | 2 486.340 | 2 100.562 | 3 871.439 | 955.303 |
1MG-AD | 2 484.530 | 1 885.088 | 3 798.843 | 940.949 |
1MG-A | 2 488.346 | 2 102.552 | 3 873.450 | 957.307 |
1MG-EAD | 2 484.530 | 1 885.088 | 3 875.441 | 959.307 |
1MG-NCD | 2 484.530 | 1 885.088 | 3 875.441 | 959.307 |
2MG-ADI | 2 369.833 | 1 833.238 | 3 724.999 | 943.029 |
2MG-AD | 2 484.840 | 1 889.086 | 3 802.812 | 944.950 |
2MG-A | 2 490.346 | 2 104.543 | 3 875.450 | 959.313 |
2MG-EA | 2 488.347 | 2 102.555 | 3 873.449 | 957.305 |
2MG-CD | 2 480.840 | 1 834.670 | 3 875.442 | 959.308 |
2MG-EAD | 2 478.840 | 1 880.260 | 3 873.442 | 957.308 |
表5 菊花‘绿叮当’与毛华菊杂交后代群体花部性状对应的各遗传模型的 AIC 值
Table 5 Akaike’s information criterion(AIC)values of various models for the traits in the progeny of Chrysanthemu × morifolium‘Lüdingdang’and C. vestitum
模型编码 Model code | h | a* | 舌状花瓣数 Number of ray florets | 花序直径/cm Flower head diameter |
---|---|---|---|---|
0MG | 2 486.340 | 2 100.562 | 3 871.439 | 955.303 |
1MG-AD | 2 484.530 | 1 885.088 | 3 798.843 | 940.949 |
1MG-A | 2 488.346 | 2 102.552 | 3 873.450 | 957.307 |
1MG-EAD | 2 484.530 | 1 885.088 | 3 875.441 | 959.307 |
1MG-NCD | 2 484.530 | 1 885.088 | 3 875.441 | 959.307 |
2MG-ADI | 2 369.833 | 1 833.238 | 3 724.999 | 943.029 |
2MG-AD | 2 484.840 | 1 889.086 | 3 802.812 | 944.950 |
2MG-A | 2 490.346 | 2 104.543 | 3 875.450 | 959.313 |
2MG-EA | 2 488.347 | 2 102.555 | 3 873.449 | 957.305 |
2MG-CD | 2 480.840 | 1 834.670 | 3 875.442 | 959.308 |
2MG-EAD | 2 478.840 | 1 880.260 | 3 873.442 | 957.308 |
性状Trait | 模型Model | U12(P) | U22(P) | U32(P) | nW2(P) | Dn(P) |
---|---|---|---|---|---|---|
h | 2MG-ADI | 0.453(0.501) | 0.289(0.591) | 0.210(0.647) | 0.246(0.199) | 0.058(0.096) |
a* | 2MG-ADI | 0.000(0.998) | 0.108(0.743) | 1.746(0.186) | 0.215(0.243) | 0.056(0.123) |
舌状花瓣数 Number of ray florets | 2MG-ADI | 0.710(0.399) | 0.281(0.596) | 1.307(0.253) | 0.495(0.042) | 0.068(0.031) |
花序直径 Flower head diameter | 1MG-AD | 0.017(0.897) | 0.213(0.644) | 1.809(0.179) | 0.154(0.380) | 0.042(0.387) |
表6 入选模型的适合性检验
Table 6 Test for goodness-of-fit of selected genetic model
性状Trait | 模型Model | U12(P) | U22(P) | U32(P) | nW2(P) | Dn(P) |
---|---|---|---|---|---|---|
h | 2MG-ADI | 0.453(0.501) | 0.289(0.591) | 0.210(0.647) | 0.246(0.199) | 0.058(0.096) |
a* | 2MG-ADI | 0.000(0.998) | 0.108(0.743) | 1.746(0.186) | 0.215(0.243) | 0.056(0.123) |
舌状花瓣数 Number of ray florets | 2MG-ADI | 0.710(0.399) | 0.281(0.596) | 1.307(0.253) | 0.495(0.042) | 0.068(0.031) |
花序直径 Flower head diameter | 1MG-AD | 0.017(0.897) | 0.213(0.644) | 1.809(0.179) | 0.154(0.380) | 0.042(0.387) |
遗传参数 Genetic parameter | h | a* | 舌状花瓣数 Number of ray florets | 花序直径 Flower head diameter |
---|---|---|---|---|
m | 102.30 | -5.05 | 47.03 | 4.81 |
da | 4.27 | 2.46 | 14.77 | 0.54 |
db | 4.25 | 0.77 | 14.18 | |
ha | 2.12 | 1.23 | -22.06 | -0.54 |
hb | 4.23 | 0.19 | -1.37 | |
i | -4.25 | -0.77 | 14.17 | |
jab | -4.23 | -0.19 | -1.36 | |
jba | -2.11 | 0.46 | -6.73 | |
l | -2.09 | 1.05 | 8.72 | |
主基因方差 Major-gene variance | 6.02 | 5.90 | 236.95 | 0.20 |
主基因遗传率/% Heritability | 38.74 | 90.34 | 76.20 | 41.20 |
表7 最优模型下不同性状遗传参数估计值
Table 7 Estimation of genetic parameters for different traits under the optimal model
遗传参数 Genetic parameter | h | a* | 舌状花瓣数 Number of ray florets | 花序直径 Flower head diameter |
---|---|---|---|---|
m | 102.30 | -5.05 | 47.03 | 4.81 |
da | 4.27 | 2.46 | 14.77 | 0.54 |
db | 4.25 | 0.77 | 14.18 | |
ha | 2.12 | 1.23 | -22.06 | -0.54 |
hb | 4.23 | 0.19 | -1.37 | |
i | -4.25 | -0.77 | 14.17 | |
jab | -4.23 | -0.19 | -1.36 | |
jba | -2.11 | 0.46 | -6.73 | |
l | -2.09 | 1.05 | 8.72 | |
主基因方差 Major-gene variance | 6.02 | 5.90 | 236.95 | 0.20 |
主基因遗传率/% Heritability | 38.74 | 90.34 | 76.20 | 41.20 |
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